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Protein

Exosome RNA helicase MTR4

Gene

Mtrex

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of exosome targeting complexes. Subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. Subunit of the trimeric poly(A) tail exosome targeting (PAXT) complex, a complex that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. Associated with the RNA exosome complex and involved in the 3'-processing of the 7S pre-RNA to the mature 5.8S rRNA. May be involved in pre-mRNA splicing.By similarity

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi159 – 166ATPPROSITE-ProRule annotation8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ATP-dependent RNA helicase activity Source: GO_Central
  • RNA binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionHelicase, Hydrolase
Biological processmRNA processing, mRNA splicing, rRNA processing
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Names & Taxonomyi

Protein namesi
Recommended name:
Exosome RNA helicase MTR4Curated (EC:3.6.4.13)
Alternative name(s):
ATP-dependent helicase SKIV2L2
Superkiller viralicidic activity 2-like 2
TRAMP-like complex helicase
Gene namesi
Name:Mtrex
Synonyms:Skiv2l2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1919448 Mtrex

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001020952 – 1040Exosome RNA helicase MTR4Add BLAST1039

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Modified residuei38PhosphoserineBy similarity1
Modified residuei49N6-acetyllysineBy similarity1
Modified residuei76N6-acetyllysineCombined sources1
Cross-linki356Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki682Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki721Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9CZU3
MaxQBiQ9CZU3
PaxDbiQ9CZU3
PeptideAtlasiQ9CZU3
PRIDEiQ9CZU3

PTM databases

iPTMnetiQ9CZU3
PhosphoSitePlusiQ9CZU3
SwissPalmiQ9CZU3

Expressioni

Gene expression databases

BgeeiENSMUSG00000016018 Expressed in 266 organ(s), highest expression level in indifferent gonad
GenevisibleiQ9CZU3 MM

Interactioni

Subunit structurei

Component of a TRAMP-like complex, an ATP-dependent exosome regulatory complex consisting of a helicase (MTREX), an oligadenylate polymerase (TENT4B or TENT4A), and a substrate specific RNA-binding factor (ZCCHC7 or ZCCHC8). Several TRAMP-like complexes exist with specific compositions and are associated with nuclear, or nucleolar RNA exosomes. Identified in the spliceosome C complex. Component of the poly(A) tail exosome targeting (PAXT) complex made of PABPN1, ZFC3H1 and MTREX that directs a subset of long and polyadenylated poly(A) RNAs for exosomal degradation. Component of the nuclear exosome targeting (NEXT) complex composed of MTREX, ZCCHC8, and RBM7 that directs a subset of non-coding short-lived RNAs for exosomal degradation. Interacts with ZCCHC8. Binds to ZFC3H1 and RBM7 in a RNase-insensitive manner. Interacts with EXOSC10; the interaction mediates the association of MTREX with nuclear RNA exosomes. Interacts with isoform 1 of NVL in an ATP-dependent manner; the interaction is required to associate NVL with nuclear RNA exosome. Interacts with WDR74; the interaction dissociation in a late stage of rRNA synthesis is required for appropriate maturation of pre-60S particles and depends on the ATPase activity of NVL. Interacts with MPHOSPH6.By similarity

Protein-protein interaction databases

BioGridi215213, 6 interactors
ELMiQ9CZU3
IntActiQ9CZU3, 3 interactors
MINTiQ9CZU3
STRINGi10090.ENSMUSP00000022281

Structurei

3D structure databases

ProteinModelPortaliQ9CZU3
SMRiQ9CZU3
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini146 – 302Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST157
Domaini403 – 575Helicase C-terminalPROSITE-ProRule annotationAdd BLAST173

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi250 – 253DEIH box4

Sequence similaritiesi

Belongs to the helicase family. SKI2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG0948 Eukaryota
COG4581 LUCA
GeneTreeiENSGT00820000127042
HOGENOMiHOG000163047
HOVERGENiHBG104255
InParanoidiQ9CZU3
KOiK12598
OMAiNMLYRGS
OrthoDBiEOG091G01TA
PhylomeDBiQ9CZU3
TreeFamiTF300597

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR025696 rRNA_proc-arch_dom
IPR016438 Ski2-like
IPR012961 Ski2_C
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF08148 DSHCT, 1 hit
PF00271 Helicase_C, 1 hit
PF13234 rRNA_proc-arch, 1 hit
PIRSFiPIRSF005198 Antiviral_helicase_SKI2, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM01142 DSHCT, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CZU3-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADAFGDELF SVFEDDSTSA AGAKKDKEKE KWKGPPGSAD KAGKRLDTKL
60 70 80 90 100
QSESASGGKN KRDLDVEGTD EPIFGKKPRI EDSINEDLSL ADLMPRVKVQ
110 120 130 140 150
SVETVEGCTH EVALPADEDY IPLKPRVGKA AKEYPFILDA FQREAIQCVD
160 170 180 190 200
NNQSVLVSAH TSAGKTVCAE YAIALALREK QRVIFTSPIK ALSNQKYREM
210 220 230 240 250
YEEFQDVGLM TGDVTINPTA SCLVMTTEIL RSMLYRGSEV MREVAWVIFD
260 270 280 290 300
EIHYMRDSER GVVWEETIIL LPDNVHYVFL SATIPNARQF AEWICHLHKQ
310 320 330 340 350
PCHVIYTDYR PTPLQHYIFP AGGDGLHLVV DENGDFREDN FNTAMQVLRD
360 370 380 390 400
AGDLAKGDQK GRKGGTKGPS NVFKIVKMIM ERNFQPVIIF SFSKKDCEAY
410 420 430 440 450
ALQMTKLDFN TDEEKKMVEE VFNNAIDCLS DEDKKLPQVE HVLPLLKRGI
460 470 480 490 500
GIHHGGLLPI LKETIEILFS EGLIKALFAT ETFAMGINMP ARTVLFTNAR
510 520 530 540 550
KYDGKDFRWI SSGEYIQMSG RAGRRGMDDR GIVILMVDEK MSPTIGKQLL
560 570 580 590 600
KGSADPLNSA FHLTYNMVLN LLRVEEINPE YMLEKSFYQF QHYRAIPGVV
610 620 630 640 650
EKVKNSEEQY NKIVIPNEEN VVIYYKIRQQ LAKLGKEIEE YIHKPKYCLP
660 670 680 690 700
FLQPGRLVKV KNEGDDFGWG VVVNFSKKSN VKPNSGELDP LYVVEVLLRC
710 720 730 740 750
SKESLKNSAT EAAKPAKPDE KGEMQVVPVL VHLLSAISTV RLYIPKDLRP
760 770 780 790 800
VDNRQSVLKS IQEVQRRFPD GVPLLDPIDD MGIQDQGLKK VIQKVEAFEH
810 820 830 840 850
RMYSHPLHND PNLETVYTLC ERKAQIALDI KSAKRELKKA RTVLQMDELK
860 870 880 890 900
CRKRVLRRLG FATSSDVIEM KGRVACEISS ADELLLTEMM FNGLFNDLSS
910 920 930 940 950
EQATALLSCF VFQENSSEMP KLTEQLAGPL RQMQECAKRI AKVSAEAKLE
960 970 980 990 1000
IDEETYLSSF KPHLMDVVYT WATGATFAHI CKMTDVFEGS IIRCMRRLEE
1010 1020 1030 1040
LLRQMCQAAK AIGNTELENK FAEGITKIKR DIVFAASLYL
Length:1,040
Mass (Da):117,636
Last modified:June 1, 2001 - v1
Checksum:i76C1243DB5C27F65
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012158 mRNA Translation: BAB28066.1
BC029230 mRNA Translation: AAH29230.1
CCDSiCCDS26778.1
RefSeqiNP_082427.1, NM_028151.2
UniGeneiMm.291029

Genome annotation databases

EnsembliENSMUST00000022281; ENSMUSP00000022281; ENSMUSG00000016018
GeneIDi72198
KEGGimmu:72198
UCSCiuc007rws.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK012158 mRNA Translation: BAB28066.1
BC029230 mRNA Translation: AAH29230.1
CCDSiCCDS26778.1
RefSeqiNP_082427.1, NM_028151.2
UniGeneiMm.291029

3D structure databases

ProteinModelPortaliQ9CZU3
SMRiQ9CZU3
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi215213, 6 interactors
ELMiQ9CZU3
IntActiQ9CZU3, 3 interactors
MINTiQ9CZU3
STRINGi10090.ENSMUSP00000022281

PTM databases

iPTMnetiQ9CZU3
PhosphoSitePlusiQ9CZU3
SwissPalmiQ9CZU3

Proteomic databases

EPDiQ9CZU3
MaxQBiQ9CZU3
PaxDbiQ9CZU3
PeptideAtlasiQ9CZU3
PRIDEiQ9CZU3

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022281; ENSMUSP00000022281; ENSMUSG00000016018
GeneIDi72198
KEGGimmu:72198
UCSCiuc007rws.1 mouse

Organism-specific databases

CTDi72198
MGIiMGI:1919448 Mtrex

Phylogenomic databases

eggNOGiKOG0948 Eukaryota
COG4581 LUCA
GeneTreeiENSGT00820000127042
HOGENOMiHOG000163047
HOVERGENiHBG104255
InParanoidiQ9CZU3
KOiK12598
OMAiNMLYRGS
OrthoDBiEOG091G01TA
PhylomeDBiQ9CZU3
TreeFamiTF300597

Enzyme and pathway databases

ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

ChiTaRSiSkiv2l2 mouse
PROiPR:Q9CZU3
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000016018 Expressed in 266 organ(s), highest expression level in indifferent gonad
GenevisibleiQ9CZU3 MM

Family and domain databases

CDDicd00079 HELICc, 1 hit
InterProiView protein in InterPro
IPR011545 DEAD/DEAH_box_helicase_dom
IPR014001 Helicase_ATP-bd
IPR001650 Helicase_C
IPR027417 P-loop_NTPase
IPR025696 rRNA_proc-arch_dom
IPR016438 Ski2-like
IPR012961 Ski2_C
PfamiView protein in Pfam
PF00270 DEAD, 1 hit
PF08148 DSHCT, 1 hit
PF00271 Helicase_C, 1 hit
PF13234 rRNA_proc-arch, 1 hit
PIRSFiPIRSF005198 Antiviral_helicase_SKI2, 1 hit
SMARTiView protein in SMART
SM00487 DEXDc, 1 hit
SM01142 DSHCT, 1 hit
SM00490 HELICc, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
PROSITEiView protein in PROSITE
PS51192 HELICASE_ATP_BIND_1, 1 hit
PS51194 HELICASE_CTER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMTREX_MOUSE
AccessioniPrimary (citable) accession number: Q9CZU3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 19, 2005
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 153 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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