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Protein

Nuclear distribution protein nudE homolog 1

Gene

Nde1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.1 Publication

Miscellaneous

Homozygous loss of this protein results in microcephaly that preferentially affects the cerebral cortex. Affected animals have a smaller cortex with reduced superficial cortical layers, although cortical lamination is mostly preserved. The smaller cortex size seems to reflect both reduced progenitor cell division and altered specification of cell fates following progenitor cell division.

GO - Molecular functioni

  • identical protein binding Source: MGI
  • microtubule binding Source: UniProtKB
  • protein domain specific binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein
Biological processCell cycle, Cell division, Differentiation, Mitosis, Neurogenesis

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase
R-MMU-8854518 AURKA Activation by TPX2

Names & Taxonomyi

Protein namesi
Recommended name:
Nuclear distribution protein nudE homolog 1
Short name:
NudE
Short name:
mNudE
Gene namesi
Name:Nde1
Synonyms:Nude
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:1914453 Nde1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Cytoskeleton, Kinetochore, Microtubule

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002402031 – 344Nuclear distribution protein nudE homolog 1Add BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei211PhosphoserineBy similarity1
Modified residuei215PhosphothreonineBy similarity1
Modified residuei228PhosphothreonineBy similarity1
Modified residuei239PhosphoserineBy similarity1
Modified residuei243PhosphothreonineBy similarity1
Modified residuei246Phosphothreonine1 Publication1
Lipidationi274S-palmitoyl cysteine; by ZDHHC2, ZDHHC3 and ZDHHC7By similarity1
Modified residuei282PhosphoserineBy similarity1
Modified residuei309PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylated in mitosis (By similarity). Phosphorylation at Thr-246 is essential for the G2/M transition.By similarity1 Publication

Keywords - PTMi

Lipoprotein, Palmitate, Phosphoprotein

Proteomic databases

PaxDbiQ9CZA6
PeptideAtlasiQ9CZA6
PRIDEiQ9CZA6

PTM databases

iPTMnetiQ9CZA6
PhosphoSitePlusiQ9CZA6
SwissPalmiQ9CZA6

Expressioni

Tissue specificityi

Highly expressed in ovary. Also expressed in brain, heart, kidney, large intestine, liver, lung, small intestine and testis.2 Publications

Developmental stagei

Widely expressed in the developing brain, particularly in the neural progenitor cells of ventricular zone and the intermediate zone of the cerebral cortex. Expression peaks at E11 and declines at E15 and E17. After completion of neuronal migration expression is reduced in the cortex.2 Publications

Gene expression databases

BgeeiENSMUSG00000022678 Expressed in 292 organ(s), highest expression level in cochlea
ExpressionAtlasiQ9CZA6 baseline and differential
GenevisibleiQ9CZA6 MM

Interactioni

Subunit structurei

Interacts with dynactin and PCM1 (By similarity). Self-associates. Interacts with CENPF, LIS1, CNTRL, dynein, tubulin gamma, PAFAH1B1, PCNT, SLMAP and TCP1. Interacts with ZNF365 (By similarity).By similarity

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212013, 17 interactors
DIPiDIP-54638N
IntActiQ9CZA6, 19 interactors
MINTiQ9CZA6
STRINGi10090.ENSMUSP00000023359

Structurei

3D structure databases

ProteinModelPortaliQ9CZA6
SMRiQ9CZA6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 93Self-associationAdd BLAST93
Regioni88 – 156Interaction with PAFAH1B1Add BLAST69
Regioni167 – 290Interaction with CENPFAdd BLAST124

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili18 – 188Sequence analysisAdd BLAST171

Sequence similaritiesi

Belongs to the nudE family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG1853 Eukaryota
ENOG410XPMP LUCA
GeneTreeiENSGT00390000000111
HOGENOMiHOG000280681
HOVERGENiHBG082010
InParanoidiQ9CZA6
KOiK16738
OMAiFRCICER
OrthoDBiEOG091G0GBV
PhylomeDBiQ9CZA6
TreeFamiTF325693

Family and domain databases

InterProiView protein in InterPro
IPR033494 NUDE
IPR006964 NUDE_dom
PANTHERiPTHR10921 PTHR10921, 1 hit
PfamiView protein in Pfam
PF04880 NUDE_C, 1 hit

Sequences (4+)i

Sequence statusi: Complete.

This entry describes 4 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9CZA6-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MEDSGKTFES EEEETNYWRD LAMTYKQRAE NTQEELREFQ EGSREYEAEL
60 70 80 90 100
EAQLQQIETR NRDLLSENNR LRMELESVKE KFEMQHSEGY RQISALEDDL
110 120 130 140 150
AQTKAIKDQL QKYIRELEQA NDDLERAKRA TIMSLEDFEQ RLNQAIERNA
160 170 180 190 200
FLESELDEKE NLLESVQRLK DEARDLRQEL AVQQKQDKPR TPMPGSGQAK
210 220 230 240 250
RTDMAVQATG SVPSTPVAHR GPSSGLNTPG MFRRGLDSST SGTPLTPAAR
260 270 280 290 300
ISALNIVGDL LRKVGALESK LASCRNFMYD QSPSRTSGPA SGRGTKNRDG
310 320 330 340
VDRRPGSTSV GDKGSGKRLE FGKPASEPAS PALPSAQGVV KLLL
Length:344
Mass (Da):38,523
Last modified:June 1, 2001 - v1
Checksum:iC0A339C4F51EF8D9
GO
Isoform 2 (identifier: Q9CZA6-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     315-317: SGK → REN
     318-344: Missing.

Note: No experimental confirmation available.
Show »
Length:317
Mass (Da):35,892
Checksum:i714BD7DD448D4EB3
GO
Isoform 3 (identifier: Q9CZA6-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     316-317: GK → LP
     318-344: Missing.

Note: No experimental confirmation available.
Show »
Length:317
Mass (Da):35,790
Checksum:i6267E66D448D4EB3
GO
Isoform 4 (identifier: Q9CZA6-4) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-132: Missing.

Show »
Length:212
Mass (Da):22,682
Checksum:iD9D5D1046E384F2E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Q325F6Q325_MOUSE
Nuclear distribution protein nudE h...
Nde1
175Annotation score:
D6RJ36D6RJ36_MOUSE
Nuclear distribution protein nudE h...
Nde1
88Annotation score:

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0193061 – 132Missing in isoform 4. 1 PublicationAdd BLAST132
Alternative sequenceiVSP_019307315 – 317SGK → REN in isoform 2. 1 Publication3
Alternative sequenceiVSP_019308316 – 317GK → LP in isoform 3. 1 Publication2
Alternative sequenceiVSP_019309318 – 344Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST27

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322073 mRNA Translation: AAK08067.1
AF290473 mRNA Translation: AAG10062.1
AK012830 mRNA Translation: BAB28499.1
AK037289 mRNA Translation: BAE20509.1
AK146982 mRNA Translation: BAE27585.1
AK150826 mRNA Translation: BAE29888.1
AK169743 mRNA Translation: BAE41342.1
BC023267 mRNA Translation: AAH23267.1
CCDSiCCDS37263.2 [Q9CZA6-1]
CCDS49770.1 [Q9CZA6-3]
CCDS70688.1 [Q9CZA6-2]
RefSeqiNP_001107557.1, NM_001114085.1 [Q9CZA6-3]
NP_001272432.1, NM_001285503.1 [Q9CZA6-2]
NP_001272433.1, NM_001285504.1 [Q9CZA6-4]
NP_075806.2, NM_023317.2 [Q9CZA6-1]
XP_011244302.1, XM_011246000.1 [Q9CZA6-4]
UniGeneiMm.24105

Genome annotation databases

EnsembliENSMUST00000023359; ENSMUSP00000023359; ENSMUSG00000022678 [Q9CZA6-1]
ENSMUST00000115795; ENSMUSP00000111461; ENSMUSG00000022678 [Q9CZA6-2]
ENSMUST00000117958; ENSMUSP00000112817; ENSMUSG00000022678 [Q9CZA6-3]
GeneIDi67203
KEGGimmu:67203
UCSCiuc007ygy.2 mouse [Q9CZA6-1]
uc007yha.2 mouse [Q9CZA6-3]
uc007yhb.3 mouse [Q9CZA6-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322073 mRNA Translation: AAK08067.1
AF290473 mRNA Translation: AAG10062.1
AK012830 mRNA Translation: BAB28499.1
AK037289 mRNA Translation: BAE20509.1
AK146982 mRNA Translation: BAE27585.1
AK150826 mRNA Translation: BAE29888.1
AK169743 mRNA Translation: BAE41342.1
BC023267 mRNA Translation: AAH23267.1
CCDSiCCDS37263.2 [Q9CZA6-1]
CCDS49770.1 [Q9CZA6-3]
CCDS70688.1 [Q9CZA6-2]
RefSeqiNP_001107557.1, NM_001114085.1 [Q9CZA6-3]
NP_001272432.1, NM_001285503.1 [Q9CZA6-2]
NP_001272433.1, NM_001285504.1 [Q9CZA6-4]
NP_075806.2, NM_023317.2 [Q9CZA6-1]
XP_011244302.1, XM_011246000.1 [Q9CZA6-4]
UniGeneiMm.24105

3D structure databases

ProteinModelPortaliQ9CZA6
SMRiQ9CZA6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212013, 17 interactors
DIPiDIP-54638N
IntActiQ9CZA6, 19 interactors
MINTiQ9CZA6
STRINGi10090.ENSMUSP00000023359

PTM databases

iPTMnetiQ9CZA6
PhosphoSitePlusiQ9CZA6
SwissPalmiQ9CZA6

Proteomic databases

PaxDbiQ9CZA6
PeptideAtlasiQ9CZA6
PRIDEiQ9CZA6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000023359; ENSMUSP00000023359; ENSMUSG00000022678 [Q9CZA6-1]
ENSMUST00000115795; ENSMUSP00000111461; ENSMUSG00000022678 [Q9CZA6-2]
ENSMUST00000117958; ENSMUSP00000112817; ENSMUSG00000022678 [Q9CZA6-3]
GeneIDi67203
KEGGimmu:67203
UCSCiuc007ygy.2 mouse [Q9CZA6-1]
uc007yha.2 mouse [Q9CZA6-3]
uc007yhb.3 mouse [Q9CZA6-2]

Organism-specific databases

CTDi54820
MGIiMGI:1914453 Nde1

Phylogenomic databases

eggNOGiKOG1853 Eukaryota
ENOG410XPMP LUCA
GeneTreeiENSGT00390000000111
HOGENOMiHOG000280681
HOVERGENiHBG082010
InParanoidiQ9CZA6
KOiK16738
OMAiFRCICER
OrthoDBiEOG091G0GBV
PhylomeDBiQ9CZA6
TreeFamiTF325693

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase
R-MMU-8854518 AURKA Activation by TPX2

Miscellaneous databases

PROiPR:Q9CZA6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022678 Expressed in 292 organ(s), highest expression level in cochlea
ExpressionAtlasiQ9CZA6 baseline and differential
GenevisibleiQ9CZA6 MM

Family and domain databases

InterProiView protein in InterPro
IPR033494 NUDE
IPR006964 NUDE_dom
PANTHERiPTHR10921 PTHR10921, 1 hit
PfamiView protein in Pfam
PF04880 NUDE_C, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDE1_MOUSE
AccessioniPrimary (citable) accession number: Q9CZA6
Secondary accession number(s): Q3UBS6, Q3UIC1, Q9ERR0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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