UniProtKB - Q9CYR0 (SSBP_MOUSE)
Protein
Single-stranded DNA-binding protein, mitochondrial
Gene
Ssbp1
Organism
Mus musculus (Mouse)
Status
Functioni
Binds preferentially and cooperatively to pyrimidine rich single-stranded DNA (ss-DNA). In vitro, required to maintain the copy number of mitochondrial DNA (mtDNA) and plays crucial roles during mtDNA replication that stimulate activity of the replisome components POLG and TWNK at the replication fork. Promotes the activity of the gamma complex polymerase POLG, largely by organizing the template DNA and eliminating secondary structures to favor ss-DNA conformations that facilitate POLG activity. In addition it is able to promote the 5'-3' unwinding activity of the mtDNA helicase TWNK. May also function in mtDNA repair.By similarity
GO - Molecular functioni
- chromatin binding Source: UniProtKB
- identical protein binding Source: Ensembl
- single-stranded DNA binding Source: MGI
GO - Biological processi
- DNA unwinding involved in DNA replication Source: MGI
- mitochondrial DNA replication Source: GO_Central
- mitochondrion morphogenesis Source: MGI
- positive regulation of DNA helicase activity Source: MGI
- positive regulation of helicase activity Source: MGI
Keywordsi
Molecular function | DNA-binding |
Biological process | DNA replication |
Names & Taxonomyi
Protein namesi | Recommended name: Single-stranded DNA-binding protein, mitochondrialShort name: Mt-SSB Short name: MtSSB |
Gene namesi | Name:Ssbp1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920040, Ssbp1 |
Subcellular locationi
Mitochondrion
- Mitochondrion By similarity
- mitochondrion nucleoid By similarity
Mitochondrion
- mitochondrial nucleoid Source: UniProtKB
- mitochondrion Source: MGI
Other locations
- nucleoid Source: GO_Central
Keywords - Cellular componenti
Mitochondrion, Mitochondrion nucleoidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 16 | MitochondrionBy similarityAdd BLAST | 16 | |
ChainiPRO_0000033264 | 17 – 152 | Single-stranded DNA-binding protein, mitochondrialAdd BLAST | 136 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 67 | PhosphoserineCombined sources | 1 | |
Modified residuei | 79 | PhosphoserineBy similarity | 1 | |
Modified residuei | 113 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 122 | N6-succinyllysineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9CYR0 |
MaxQBi | Q9CYR0 |
PaxDbi | Q9CYR0 |
PeptideAtlasi | Q9CYR0 |
PRIDEi | Q9CYR0 |
ProteomicsDBi | 263350 |
PTM databases
iPTMneti | Q9CYR0 |
PhosphoSitePlusi | Q9CYR0 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029911, Expressed in epiblast (generic) and 175 other tissues |
ExpressionAtlasi | Q9CYR0, baseline and differential |
Genevisiblei | Q9CYR0, MM |
Interactioni
Subunit structurei
Homotetramer.
Interacts with MPG/AAG, through inhibition of its glycosylase activity it potentially prevents formation of DNA breaks in ssDNA, ensuring that base removal primarily occurs in dsDNA.
Interacts with POLDIP2.
Interacts with PRIMPOL.
By similarityGO - Molecular functioni
- identical protein binding Source: Ensembl
Protein-protein interaction databases
BioGRIDi | 238075, 7 interactors |
IntActi | Q9CYR0, 2 interactors |
STRINGi | 10090.ENSMUSP00000031971 |
Miscellaneous databases
RNActi | Q9CYR0, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 30 – 141 | SSBAdd BLAST | 112 |
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1653, Eukaryota |
GeneTreei | ENSGT00390000002796 |
InParanoidi | Q9CYR0 |
OrthoDBi | 1304218at2759 |
PhylomeDBi | Q9CYR0 |
TreeFami | TF314629 |
Family and domain databases
CDDi | cd04496, SSB_OBF, 1 hit |
HAMAPi | MF_00984, SSB, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR000424, Primosome_PriB/ssb IPR011344, ssDNA-bd |
PANTHERi | PTHR10302, PTHR10302, 1 hit |
Pfami | View protein in Pfam PF00436, SSB, 1 hit |
PIRSFi | PIRSF002070, SSB, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00621, ssb, 1 hit |
PROSITEi | View protein in PROSITE PS50935, SSB, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9CYR0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MFRRPVLQVF RQFVRHESEV ASSLVLERSL NRVQLLGRVG QDPVMRQVEG
60 70 80 90 100
KNPVTIFSLA TNEMWRSGDS EVYQMGDVSQ KTTWHRISVF RPGLRDVAYQ
110 120 130 140 150
YVKKGARIFV EGKVDYGEYM DKNNVRRQAT TIIAGKKLVV HSVSGCSLEG
LA
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ8R2K3 | Q8R2K3_MOUSE | Single-stranded DNA-binding protein... | Ssbp1 | 148 | Annotation score: | ||
D3Z3Y3 | D3Z3Y3_MOUSE | Single-stranded DNA-binding protein... | Ssbp1 | 76 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013425 mRNA Translation: BAB28850.1 |
CCDSi | CCDS20030.1 |
RefSeqi | NP_082634.1, NM_028358.2 NP_997633.1, NM_212468.3 XP_006506392.1, XM_006506329.2 XP_017177153.1, XM_017321664.1 |
Genome annotation databases
Ensembli | ENSMUST00000031971; ENSMUSP00000031971; ENSMUSG00000029911 |
GeneIDi | 381760 |
KEGGi | mmu:381760 |
UCSCi | uc009bmr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK013425 mRNA Translation: BAB28850.1 |
CCDSi | CCDS20030.1 |
RefSeqi | NP_082634.1, NM_028358.2 NP_997633.1, NM_212468.3 XP_006506392.1, XM_006506329.2 XP_017177153.1, XM_017321664.1 |
3D structure databases
SMRi | Q9CYR0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 238075, 7 interactors |
IntActi | Q9CYR0, 2 interactors |
STRINGi | 10090.ENSMUSP00000031971 |
PTM databases
iPTMneti | Q9CYR0 |
PhosphoSitePlusi | Q9CYR0 |
Proteomic databases
EPDi | Q9CYR0 |
MaxQBi | Q9CYR0 |
PaxDbi | Q9CYR0 |
PeptideAtlasi | Q9CYR0 |
PRIDEi | Q9CYR0 |
ProteomicsDBi | 263350 |
Protocols and materials databases
Antibodypediai | 1260, 173 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000031971; ENSMUSP00000031971; ENSMUSG00000029911 |
GeneIDi | 381760 |
KEGGi | mmu:381760 |
UCSCi | uc009bmr.1, mouse |
Organism-specific databases
CTDi | 6742 |
MGIi | MGI:1920040, Ssbp1 |
Phylogenomic databases
eggNOGi | KOG1653, Eukaryota |
GeneTreei | ENSGT00390000002796 |
InParanoidi | Q9CYR0 |
OrthoDBi | 1304218at2759 |
PhylomeDBi | Q9CYR0 |
TreeFami | TF314629 |
Miscellaneous databases
BioGRID-ORCSi | 381760, 20 hits in 51 CRISPR screens |
ChiTaRSi | Ssbp1, mouse |
PROi | PR:Q9CYR0 |
RNActi | Q9CYR0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029911, Expressed in epiblast (generic) and 175 other tissues |
ExpressionAtlasi | Q9CYR0, baseline and differential |
Genevisiblei | Q9CYR0, MM |
Family and domain databases
CDDi | cd04496, SSB_OBF, 1 hit |
HAMAPi | MF_00984, SSB, 1 hit |
InterProi | View protein in InterPro IPR012340, NA-bd_OB-fold IPR000424, Primosome_PriB/ssb IPR011344, ssDNA-bd |
PANTHERi | PTHR10302, PTHR10302, 1 hit |
Pfami | View protein in Pfam PF00436, SSB, 1 hit |
PIRSFi | PIRSF002070, SSB, 1 hit |
SUPFAMi | SSF50249, SSF50249, 1 hit |
TIGRFAMsi | TIGR00621, ssb, 1 hit |
PROSITEi | View protein in PROSITE PS50935, SSB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SSBP_MOUSE | |
Accessioni | Q9CYR0Primary (citable) accession number: Q9CYR0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 10, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot