Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Sestrin-3

Gene

Sesn3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May function as an intracellular leucine sensor that negatively regulates the TORC1 signaling pathway (PubMed:25259925). May also regulate the insulin-receptor signaling pathway through activation of TORC2 (PubMed:25377878). This metabolic regulator may also play a role in protection against oxidative and genotoxic stresses (By similarity).By similarity2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei398Leucine; via carbonyl oxygenBy similarity1
Binding sitei463LeucineBy similarity1

GO - Molecular functioni

  • leucine binding Source: GO_Central
  • oxidoreductase activity, acting on peroxide as acceptor Source: GO_Central

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase

Enzyme and pathway databases

ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes

Names & Taxonomyi

Protein namesi
Recommended name:
Sestrin-3Curated
Gene namesi
Name:Sesn3Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1922997 Sesn3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Liver-specific Sesn3 knockout mice display insulin resistance and glucose intolerance (PubMed:25377878). Sesn2 and Sesn3 double knockout mice display insulin resistance and glucose intolerance (PubMed:22958918). Triple knockout mice lacking Sesn1, Sesn2 and Sesn3 do not display an embryonic lethal phenotype since they are born at an expected Mendelian ratio. Moreover, they are not distinguishable from their wild-type littermate. However, their survival at 10 days is dramatically affected. This is associated with a constitutive activation of TORC1 signaling in the liver, heart and skeletal muscle during postnatal fasting, that occurs between birth and suckling (PubMed:25259925).3 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002211841 – 492Sestrin-3Add BLAST492

Proteomic databases

MaxQBiQ9CYP7
PaxDbiQ9CYP7
PRIDEiQ9CYP7

PTM databases

iPTMnetiQ9CYP7
PhosphoSitePlusiQ9CYP7

Expressioni

Tissue specificityi

Detected in liver and skeletal muscles.1 Publication

Gene expression databases

BgeeiENSMUSG00000032009 Expressed in 258 organ(s), highest expression level in stria vascularis of cochlear duct
CleanExiMM_SESN3
ExpressionAtlasiQ9CYP7 baseline and differential
GenevisibleiQ9CYP7 MM

Interactioni

Subunit structurei

Interacts with the GATOR2 complex which is composed of MIOS, SEC13, SEH1L, WDR24 and WDR59; the interaction is not regulated by leucine (By similarity). Interacts with RRAGA, RRAGB, RRAGC and RRAGD; may function as a guanine nucleotide dissociation inhibitor for RRAGs and regulate them (PubMed:25259925). Interacts with the TORC2 complex; through RICTOR (PubMed:25377878).By similarity2 Publications

Protein-protein interaction databases

IntActiQ9CYP7, 1 interactor
STRINGi10090.ENSMUSP00000034507

Structurei

3D structure databases

ProteinModelPortaliQ9CYP7
SMRiQ9CYP7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni310 – 492C-terminal domain; mediates TORC1 regulationBy similarityAdd BLAST183
Regioni386 – 389Leucine-bindingBy similarity4

Domaini

The C-terminal domain may mediate interaction with GATOR2 and regulation of TORC1 signaling.By similarity

Sequence similaritiesi

Belongs to the sestrin family.Curated

Phylogenomic databases

eggNOGiKOG3746 Eukaryota
ENOG410XP7Z LUCA
GeneTreeiENSGT00440000040103
HOGENOMiHOG000232949
HOVERGENiHBG054648
InParanoidiQ9CYP7
KOiK10141
OrthoDBiEOG091G0IVA
PhylomeDBiQ9CYP7
TreeFamiTF314230

Family and domain databases

Gene3Di1.20.1290.10, 1 hit
InterProiView protein in InterPro
IPR029032 AhpD-like
IPR006730 Sestrin
PANTHERiPTHR12474 PTHR12474, 1 hit
PfamiView protein in Pfam
PF04636 PA26, 1 hit
SUPFAMiSSF69118 SSF69118, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9CYP7-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNRGGSSASA SANYLLCTNC RKVLRKDKRI RVSQPLTRGP SAFIPEKEVV
60 70 80 90 100
QANTADERTN FLVEEYSTSG RLDNITQVMS LHTQYLESFL RSQFYMLRMD
110 120 130 140 150
GPLPLPDRHY IAIMAAARHQ CSYLINMHVD EFLKTGGIAE WLNGLEYVPQ
160 170 180 190 200
RLRNLNEINK LLAHRPWLIT KEHIQKLVKT GENNWSLPEL VHAVVLLAHY
210 220 230 240 250
HALASFVFGS GINPERDPGI ANGFRLISVS SFCVCDLAND NSIENTSLAG
260 270 280 290 300
SNFGIVDSLG ELEALMERMK RLQEDREDDE TTREEMTTRF EKEKKESLFV
310 320 330 340 350
VPGETLHAFP HSDFEDDVIV TADVSRYIED PSFGYEDFAR RGEEHLPTFR
360 370 380 390 400
AQDYTWENHG FSLVNRLYSD IGHLLDEKFR MVYNLTYNTM ATHEDVDTTT
410 420 430 440 450
LRRALFNYVH CMFGIRYDDY DYGEVNQLLE RSLKVYIKTV TCYPERTTKR
460 470 480 490
MYDSYWRQFT HSEKVHVNLL LMEARMQAEL LYALRAITRH LT
Length:492
Mass (Da):57,021
Last modified:April 27, 2001 - v1
Checksum:i8E8AD9CB45656827
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YMZ5A0A0J9YMZ5_MOUSE
Sestrin-3
Sesn3
414Annotation score:
A0A140LIE1A0A140LIE1_MOUSE
Sestrin-3
Sesn3
353Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017464 mRNA Translation: BAB30755.1
AK155398 mRNA Translation: BAE33242.1
CCDSiCCDS22820.1
RefSeqiNP_084537.2, NM_030261.4
UniGeneiMm.325126
Mm.439776

Genome annotation databases

EnsembliENSMUST00000208222; ENSMUSP00000146362; ENSMUSG00000032009
GeneIDi75747
KEGGimmu:75747
UCSCiuc009oej.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017464 mRNA Translation: BAB30755.1
AK155398 mRNA Translation: BAE33242.1
CCDSiCCDS22820.1
RefSeqiNP_084537.2, NM_030261.4
UniGeneiMm.325126
Mm.439776

3D structure databases

ProteinModelPortaliQ9CYP7
SMRiQ9CYP7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CYP7, 1 interactor
STRINGi10090.ENSMUSP00000034507

PTM databases

iPTMnetiQ9CYP7
PhosphoSitePlusiQ9CYP7

Proteomic databases

MaxQBiQ9CYP7
PaxDbiQ9CYP7
PRIDEiQ9CYP7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000208222; ENSMUSP00000146362; ENSMUSG00000032009
GeneIDi75747
KEGGimmu:75747
UCSCiuc009oej.1 mouse

Organism-specific databases

CTDi143686
MGIiMGI:1922997 Sesn3

Phylogenomic databases

eggNOGiKOG3746 Eukaryota
ENOG410XP7Z LUCA
GeneTreeiENSGT00440000040103
HOGENOMiHOG000232949
HOVERGENiHBG054648
InParanoidiQ9CYP7
KOiK10141
OrthoDBiEOG091G0IVA
PhylomeDBiQ9CYP7
TreeFamiTF314230

Enzyme and pathway databases

ReactomeiR-MMU-5628897 TP53 Regulates Metabolic Genes

Miscellaneous databases

ChiTaRSiSesn3 mouse
PROiPR:Q9CYP7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032009 Expressed in 258 organ(s), highest expression level in stria vascularis of cochlear duct
CleanExiMM_SESN3
ExpressionAtlasiQ9CYP7 baseline and differential
GenevisibleiQ9CYP7 MM

Family and domain databases

Gene3Di1.20.1290.10, 1 hit
InterProiView protein in InterPro
IPR029032 AhpD-like
IPR006730 Sestrin
PANTHERiPTHR12474 PTHR12474, 1 hit
PfamiView protein in Pfam
PF04636 PA26, 1 hit
SUPFAMiSSF69118 SSF69118, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSESN3_MOUSE
AccessioniPrimary (citable) accession number: Q9CYP7
Secondary accession number(s): Q3U2A0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 7, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again