UniProtKB - Q9CYK2 (QPCT_MOUSE)
Glutaminyl-peptide cyclotransferase
Qpct
Functioni
Responsible for the biosynthesis of pyroglutamyl peptides. Has a bias against acidic and tryptophan residues adjacent to the N-terminal glutaminyl residue and a lack of importance of chain length after the second residue (By similarity).
By similarityCaution
Catalytic activityi
- EC:2.3.2.5
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 160 | ZincCombined sources1 Publication | 1 | |
Active sitei | 202 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 203 | ZincCombined sources1 Publication | 1 | |
Active sitei | 249 | Proton acceptorBy similarity | 1 | |
Metal bindingi | 331 | ZincCombined sources1 Publication | 1 |
GO - Molecular functioni
- glutaminyl-peptide cyclotransferase activity Source: UniProtKB
- zinc ion binding Source: UniProtKB
GO - Biological processi
- peptidyl-pyroglutamic acid biosynthetic process, using glutaminyl-peptide cyclotransferase Source: UniProtKB
Keywordsi
Molecular function | Acyltransferase, Transferase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 2.3.2.5, 3474 |
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Protein family/group databases
MEROPSi | M28.979 |
Names & Taxonomyi
Protein namesi | Recommended name: Glutaminyl-peptide cyclotransferase (EC:2.3.2.5)Alternative name(s): Glutaminyl cyclase Short name: QC Glutaminyl-tRNA cyclotransferase |
Gene namesi | Name:Qpct |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1917786, Qpct |
Subcellular locationi
Extracellular region or secreted
- Secreted By similarity
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Keywords - Cellular componenti
SecretedPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 35 | Sequence analysisAdd BLAST | 35 | |
ChainiPRO_0000022196 | 36 – 362 | Glutaminyl-peptide cyclotransferaseAdd BLAST | 327 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 29 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 50 | N-linked (GlcNAc...) asparagineCombined sources1 Publication | 1 | |
Disulfide bondi | 140 ↔ 165 | Combined sources1 Publication |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
MaxQBi | Q9CYK2 |
PaxDbi | Q9CYK2 |
PeptideAtlasi | Q9CYK2 |
PRIDEi | Q9CYK2 |
ProteomicsDBi | 300333 [Q9CYK2-1] 300334 [Q9CYK2-2] |
PTM databases
GlyGeni | Q9CYK2, 2 sites |
iPTMneti | Q9CYK2 |
PhosphoSitePlusi | Q9CYK2 |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038732 |
Chemistry databases
BindingDBi | Q9CYK2 |
Miscellaneous databases
RNActi | Q9CYK2, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9CYK2 |
SMRi | Q9CYK2 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG3946, Eukaryota |
InParanoidi | Q9CYK2 |
OrthoDBi | 1257754at2759 |
PhylomeDBi | Q9CYK2 |
TreeFami | TF315071 |
Family and domain databases
CDDi | cd03880, M28_QC_like, 1 hit |
InterProi | View protein in InterPro IPR037457, M28_QC IPR007484, Peptidase_M28 IPR040234, QC/QCL |
PANTHERi | PTHR12283, PTHR12283, 1 hit |
Pfami | View protein in Pfam PF04389, Peptidase_M28, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAGSEDKRVV GTLHLLLLQA TVLSLTAGNL SLVSAAWTQE KNHHQPAHLN
60 70 80 90 100
SSSLQQVAEG TSISEMWQND LRPLLIERYP GSPGSYSARQ HIMQRIQRLQ
110 120 130 140 150
AEWVVEVDTF LSRTPYGYRS FSNIISTLNP EAKRHLVLAC HYDSKYFPRW
160 170 180 190 200
DSRVFVGATD SAVPCAMMLE LARALDKKLH SLKDVSGSKP DLSLRLIFFD
210 220 230 240 250
GEEAFHHWSP QDSLYGSRHL AQKMASSPHP PGSRGTNQLD GMDLLVLLDL
260 270 280 290 300
IGAANPTFPN FFPKTTRWFN RLQAIEKELY ELGLLKDHSL ERKYFQNFGY
310 320 330 340 350
GNIIQDDHIP FLRKGVPVLH LIASPFPEVW HTMDDNEENL HASTIDNLNK
360
IIQVFVLEYL HL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0R4J0G6 | A0A0R4J0G6_MOUSE | Glutaminyl-peptide cyclotransferase | Qpct | 362 | Annotation score: | ||
A0A3B2W7G1 | A0A3B2W7G1_MOUSE | Glutaminyl-peptide cyclotransferase | Qpct | 313 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 8 | R → L in BAC29748 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 8 | R → L in BAC32556 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 8 | R → L in BAB30831 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 31 | S → N in AAH20023 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 35 | A → G in AAH20023 (PubMed:15489334).Curated | 1 | |
Sequence conflicti | 213 | S → T in BAB30831 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 326 | F → S in BAB30831 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_013911 | 42 – 90 | Missing in isoform 2. 1 PublicationAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK037197 mRNA Translation: BAC29748.1 AK045974 mRNA Translation: BAC32556.1 AK017598 mRNA Translation: BAB30831.1 BC020023 mRNA Translation: AAH20023.1 |
CCDSi | CCDS28983.1 [Q9CYK2-1] CCDS89158.1 [Q9CYK2-2] |
RefSeqi | NP_001303658.1, NM_001316729.1 NP_081731.1, NM_027455.3 |
Genome annotation databases
GeneIDi | 70536 |
KEGGi | mmu:70536 |
UCSCi | uc008dpv.1, mouse [Q9CYK2-1] uc008dpw.1, mouse [Q9CYK2-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK037197 mRNA Translation: BAC29748.1 AK045974 mRNA Translation: BAC32556.1 AK017598 mRNA Translation: BAB30831.1 BC020023 mRNA Translation: AAH20023.1 |
CCDSi | CCDS28983.1 [Q9CYK2-1] CCDS89158.1 [Q9CYK2-2] |
RefSeqi | NP_001303658.1, NM_001316729.1 NP_081731.1, NM_027455.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3SI1 | X-ray | 2.90 | A | 36-362 | [»] | |
3SI2 | X-ray | 1.80 | A | 36-362 | [»] | |
AlphaFoldDBi | Q9CYK2 | |||||
SMRi | Q9CYK2 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000038732 |
Chemistry databases
BindingDBi | Q9CYK2 |
ChEMBLi | CHEMBL4105900 |
Protein family/group databases
MEROPSi | M28.979 |
PTM databases
GlyGeni | Q9CYK2, 2 sites |
iPTMneti | Q9CYK2 |
PhosphoSitePlusi | Q9CYK2 |
Proteomic databases
MaxQBi | Q9CYK2 |
PaxDbi | Q9CYK2 |
PeptideAtlasi | Q9CYK2 |
PRIDEi | Q9CYK2 |
ProteomicsDBi | 300333 [Q9CYK2-1] 300334 [Q9CYK2-2] |
Protocols and materials databases
DNASUi | 70536 |
Genome annotation databases
GeneIDi | 70536 |
KEGGi | mmu:70536 |
UCSCi | uc008dpv.1, mouse [Q9CYK2-1] uc008dpw.1, mouse [Q9CYK2-2] |
Organism-specific databases
CTDi | 25797 |
MGIi | MGI:1917786, Qpct |
Phylogenomic databases
eggNOGi | KOG3946, Eukaryota |
InParanoidi | Q9CYK2 |
OrthoDBi | 1257754at2759 |
PhylomeDBi | Q9CYK2 |
TreeFami | TF315071 |
Enzyme and pathway databases
BRENDAi | 2.3.2.5, 3474 |
Reactomei | R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 70536, 2 hits in 75 CRISPR screens |
ChiTaRSi | Qpct, mouse |
PROi | PR:Q9CYK2 |
RNActi | Q9CYK2, protein |
SOURCEi | Search... |
Family and domain databases
CDDi | cd03880, M28_QC_like, 1 hit |
InterProi | View protein in InterPro IPR037457, M28_QC IPR007484, Peptidase_M28 IPR040234, QC/QCL |
PANTHERi | PTHR12283, PTHR12283, 1 hit |
Pfami | View protein in Pfam PF04389, Peptidase_M28, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | QPCT_MOUSE | |
Accessioni | Q9CYK2Primary (citable) accession number: Q9CYK2 Secondary accession number(s): Q8BH20, Q8VE07 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 7, 2005 |
Last sequence update: | June 7, 2005 | |
Last modified: | May 25, 2022 | |
This is version 127 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families