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Protein

Mitochondrial import receptor subunit TOM34

Gene

Tomm34

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in the import of cytosolically synthesized preproteins into mitochondria. Binds the mature portion of precursor proteins. Interacts with cellular components, and possesses weak ATPase activity. May be a chaperone-like protein that helps to keep newly synthesized precursors in an unfolded import compatible state (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChaperone

Names & Taxonomyi

Protein namesi
Recommended name:
Mitochondrial import receptor subunit TOM34
Alternative name(s):
Translocase of outer membrane 34 kDa subunit
Gene namesi
Name:Tomm34
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1914395 Tomm34

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane, Mitochondrion, Mitochondrion outer membrane

Pathology & Biotechi

Disruption phenotypei

Mice are viable, grow normally and both males and females are fertile. There is no effect on spermatogenesis or protein import into mitochondria.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001063351 – 309Mitochondrial import receptor subunit TOM34Add BLAST309

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei8PhosphoserineCombined sources1
Modified residuei160PhosphoserineBy similarity1
Modified residuei186PhosphoserineCombined sources1
Cross-linki197Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Isoform 2 (identifier: Q9CYG7-2)
Modified residuei8PhosphoserineCombined sources1

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9CYG7
PaxDbiQ9CYG7
PeptideAtlasiQ9CYG7
PRIDEiQ9CYG7

PTM databases

iPTMnetiQ9CYG7
PhosphoSitePlusiQ9CYG7

Expressioni

Tissue specificityi

Isoform 1 is ubiquitously expressed while isoform 2 is expressed only in mature testicular germ cells. Isoform 1 is expressed in all testicular cells. Isoform 2 is highly expressed in early to late pachytene cells but expression is significantly decreased in round spermatid cells.1 Publication

Developmental stagei

Isoform 2 is hardly detectable in testis at 1 week post partum but expression gradually increases for 2 to 4 weeks post partum. Isoform 1 is detected during this period but its expression does not change.1 Publication

Gene expression databases

BgeeiENSMUSG00000018322 Expressed in 283 organ(s), highest expression level in blood
CleanExiMM_TOMM34
GenevisibleiQ9CYG7 MM

Interactioni

Subunit structurei

Interacts with HSP90A, VCP, ATP6M, KIAA0665, AMPK, and DMAP1 through its TPR repeat.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211975, 1 interactor
IntActiQ9CYG7, 1 interactor
STRINGi10090.ENSMUSP00000018466

Structurei

3D structure databases

ProteinModelPortaliQ9CYG7
SMRiQ9CYG7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati9 – 42TPR 1Add BLAST34
Repeati51 – 84TPR 2Add BLAST34
Repeati85 – 118TPR 3Add BLAST34
Repeati193 – 226TPR 4Add BLAST34
Repeati227 – 260TPR 5Add BLAST34
Repeati261 – 294TPR 6Add BLAST34

Sequence similaritiesi

Belongs to the Tom34 family.Curated

Keywords - Domaini

Repeat, TPR repeat

Phylogenomic databases

eggNOGiKOG1124 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00900000140844
HOVERGENiHBG105545
InParanoidiQ9CYG7
KOiK17766
OMAiQSFRNGQ
OrthoDBiEOG091G0FFR
PhylomeDBiQ9CYG7
TreeFamiTF106202

Family and domain databases

Gene3Di1.25.40.10, 2 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 2 hits

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Note: Isoforms 1 and 2 are produced by 2 alternative initial coding exons with very similar sequences which differ at only 4 positions.
Isoform 1 (identifier: Q9CYG7-1) [UniParc]FASTAAdd to basket
Also known as: Tomm34a

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAPKVSDSVE QLRAAGNQNF RNGQYGEASA LYERALRLLQ ARGSADPEEE
60 70 80 90 100
SVLYSNRAAC YLKDGNCTDC IKDCTSALAL VPFSIKPLLR RASAYEALEK
110 120 130 140 150
YALAYVDYKT VLQIDNSVAS ALEGINRITR ALMDSLGPEW RLKLPPIPVV
160 170 180 190 200
PVSAQKRWNS LPSDNHKETA KTKSKEATAT KSRVPSAGDV ERAKALKEEG
210 220 230 240 250
NDLVKKGNHK KAIEKYSESL LCSSLESATY SNRALCHLVL KQYKEAVKDC
260 270 280 290 300
TEALKLDGKN VKAFYRRAQA YKALKDYKSS LSDISSLLQI EPRNGPAQKL

RQEVNQNMN
Length:309
Mass (Da):34,278
Last modified:June 1, 2001 - v1
Checksum:i4367551F4063278A
GO
Isoform 2 (identifier: Q9CYG7-2) [UniParc]FASTAAdd to basket
Also known as: Tomm34b

The sequence of this isoform differs from the canonical sequence as follows:
     1-26: MAPKVSDSVEQLRAAGNQNFRNGQYG → MAPKLSDSVEELRAAGNQSFRNGQYA

Show »
Length:309
Mass (Da):34,280
Checksum:i3DC7545AC92EA6AC
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti59A → S in BAC57494 (PubMed:12801914).Curated1
Sequence conflicti59A → S in BAC57495 (PubMed:12801914).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0350441 – 26MAPKV…NGQYG → MAPKLSDSVEELRAAGNQSF RNGQYA in isoform 2. 1 PublicationAdd BLAST26

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087254 Genomic DNA Translation: BAC57494.1
AB087254 Genomic DNA Translation: BAC57495.1
AK011786 mRNA Translation: BAB27840.1
AK017699 mRNA Translation: BAB30882.1
AK075873 mRNA Translation: BAC36020.1
AK168329 mRNA Translation: BAE40267.1
AL591542 Genomic DNA Translation: CAM20764.1
AL591542 Genomic DNA Translation: CAM20765.1
BC018278 mRNA Translation: AAH18278.1
CCDSiCCDS17020.1 [Q9CYG7-2]
CCDS71187.1 [Q9CYG7-1]
RefSeqiNP_001278084.1, NM_001291155.1 [Q9CYG7-1]
NP_080272.1, NM_025996.5 [Q9CYG7-2]
XP_006500130.1, XM_006500067.1 [Q9CYG7-2]
UniGeneiMm.23173

Genome annotation databases

EnsembliENSMUST00000018466; ENSMUSP00000018466; ENSMUSG00000018322 [Q9CYG7-2]
ENSMUST00000109384; ENSMUSP00000105010; ENSMUSG00000018322 [Q9CYG7-1]
GeneIDi67145
KEGGimmu:67145
UCSCiuc008ntr.2 mouse [Q9CYG7-2]
uc008nts.2 mouse [Q9CYG7-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB087254 Genomic DNA Translation: BAC57494.1
AB087254 Genomic DNA Translation: BAC57495.1
AK011786 mRNA Translation: BAB27840.1
AK017699 mRNA Translation: BAB30882.1
AK075873 mRNA Translation: BAC36020.1
AK168329 mRNA Translation: BAE40267.1
AL591542 Genomic DNA Translation: CAM20764.1
AL591542 Genomic DNA Translation: CAM20765.1
BC018278 mRNA Translation: AAH18278.1
CCDSiCCDS17020.1 [Q9CYG7-2]
CCDS71187.1 [Q9CYG7-1]
RefSeqiNP_001278084.1, NM_001291155.1 [Q9CYG7-1]
NP_080272.1, NM_025996.5 [Q9CYG7-2]
XP_006500130.1, XM_006500067.1 [Q9CYG7-2]
UniGeneiMm.23173

3D structure databases

ProteinModelPortaliQ9CYG7
SMRiQ9CYG7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211975, 1 interactor
IntActiQ9CYG7, 1 interactor
STRINGi10090.ENSMUSP00000018466

PTM databases

iPTMnetiQ9CYG7
PhosphoSitePlusiQ9CYG7

Proteomic databases

EPDiQ9CYG7
PaxDbiQ9CYG7
PeptideAtlasiQ9CYG7
PRIDEiQ9CYG7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000018466; ENSMUSP00000018466; ENSMUSG00000018322 [Q9CYG7-2]
ENSMUST00000109384; ENSMUSP00000105010; ENSMUSG00000018322 [Q9CYG7-1]
GeneIDi67145
KEGGimmu:67145
UCSCiuc008ntr.2 mouse [Q9CYG7-2]
uc008nts.2 mouse [Q9CYG7-1]

Organism-specific databases

CTDi10953
MGIiMGI:1914395 Tomm34

Phylogenomic databases

eggNOGiKOG1124 Eukaryota
COG0457 LUCA
GeneTreeiENSGT00900000140844
HOVERGENiHBG105545
InParanoidiQ9CYG7
KOiK17766
OMAiQSFRNGQ
OrthoDBiEOG091G0FFR
PhylomeDBiQ9CYG7
TreeFamiTF106202

Miscellaneous databases

ChiTaRSiTomm34 mouse
PROiPR:Q9CYG7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000018322 Expressed in 283 organ(s), highest expression level in blood
CleanExiMM_TOMM34
GenevisibleiQ9CYG7 MM

Family and domain databases

Gene3Di1.25.40.10, 2 hits
InterProiView protein in InterPro
IPR013026 TPR-contain_dom
IPR011990 TPR-like_helical_dom_sf
IPR001440 TPR_1
IPR019734 TPR_repeat
PfamiView protein in Pfam
PF00515 TPR_1, 1 hit
PF13181 TPR_8, 1 hit
SMARTiView protein in SMART
SM00028 TPR, 6 hits
SUPFAMiSSF48452 SSF48452, 2 hits
PROSITEiView protein in PROSITE
PS50005 TPR, 6 hits
PS50293 TPR_REGION, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiTOM34_MOUSE
AccessioniPrimary (citable) accession number: Q9CYG7
Secondary accession number(s): A2A5N5
, A2A5N6, Q3THD0, Q812F1, Q812F2, Q9D059
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 19, 2002
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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