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Protein

m7GpppN-mRNA hydrolase

Gene

Dcp2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs (PubMed:21070968). Removes the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP (PubMed:21070968). Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay (By similarity). Plays a role in replication-dependent histone mRNA degradation (By similarity). Has higher activity towards mRNAs that lack a poly(A) tail (PubMed:21070968). Has no activity towards a cap structure lacking an RNA moiety (PubMed:21070968).By similarity1 Publication

Catalytic activityi

5'-(N7-methylguanosine 5'-triphospho)-[mRNA] + H2O = N7-methylguanosine 5'-diphosphate + 5'-phospho-[mRNA].1 Publication

Cofactori

Mn2+By similarity, Mg2+By similarityNote: Mn2+ ion is required for highest activity. Can also utilize magnesium ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144ManganeseBy similarity1
Metal bindingi148ManganeseBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, RNA-binding
Biological processNonsense-mediated mRNA decay
LigandManganese, Metal-binding

Enzyme and pathway databases

BRENDAi3.6.1.62 3474
ReactomeiR-MMU-430039 mRNA decay by 5' to 3' exoribonuclease
R-MMU-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-MMU-450604 KSRP (KHSRP) binds and destabilizes mRNA

Names & Taxonomyi

Protein namesi
Recommended name:
m7GpppN-mRNA hydrolaseCurated (EC:3.6.1.621 Publication)
Alternative name(s):
mRNA-decapping enzyme 2
Gene namesi
Name:Dcp2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1917890 Dcp2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000570521 – 422m7GpppN-mRNA hydrolaseAdd BLAST422

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei246PhosphoserineCombined sources1
Modified residuei247PhosphoserineCombined sources1
Modified residuei249PhosphoserineCombined sources1
Modified residuei276PhosphoserineBy similarity1
Modified residuei284PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9CYC6
MaxQBiQ9CYC6
PaxDbiQ9CYC6
PRIDEiQ9CYC6

PTM databases

iPTMnetiQ9CYC6
PhosphoSitePlusiQ9CYC6

Expressioni

Tissue specificityi

Strongly expressed in brain and testis. Weakly expressed in lung. Not detected in heart, liver, kidney and muscle (at protein level).1 Publication

Developmental stagei

Strongly expressed in brain, heart, liver at 14.5 and 16.5 dpc. Strongly expressed in brain at 20 dpc. Weakly expressed in heart and liver at 20 dpc (at protein level).1 Publication

Gene expression databases

BgeeiENSMUSG00000024472 Expressed in 238 organ(s), highest expression level in blastocyst
CleanExiMM_DCP2
ExpressionAtlasiQ9CYC6 baseline and differential
GenevisibleiQ9CYC6 MM

Interactioni

Subunit structurei

Found in a mRNA decay complex with LSM1, LSM3, LSM4, EXOSC2, EXOSC4, EXOSC10, PARN, XRN1, CNOT6, UPF1, UPF2 and UPF3B. Forms a complex with DCP1A, EDC3, DDX6 and EDC4/HEDLS, within this complex directly interacts with EDC4/HEDLS. Interacts with DPC1B, UPF1, UPF2 and UPF3B. Associates with polysomes. Interacts (via N-terminus and C-terminus) with TRIM21 (via N-terminus and C-terminus). Interacts with LIMD1, WTIP and AJUBA. Interacts with DDX17 in an RNA-dependent manner. Interacts with ZC3HAV1. Interacts with APOBEC3G in an RNA-dependent manner. Interacts with ZFP36L1 (via N-terminus). Interacts with NBDY.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025350

Structurei

3D structure databases

ProteinModelPortaliQ9CYC6
SMRiQ9CYC6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini95 – 226Nudix hydrolasePROSITE-ProRule annotationAdd BLAST132

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi129 – 150Nudix boxAdd BLAST22

Sequence similaritiesi

Belongs to the Nudix hydrolase family. DCP2 subfamily.Curated

Phylogenomic databases

eggNOGiKOG2937 Eukaryota
COG0494 LUCA
GeneTreeiENSGT00390000018878
HOGENOMiHOG000005974
HOVERGENiHBG051321
InParanoidiQ9CYC6
KOiK12613
OMAiVHSQPAK
OrthoDBiEOG091G05Q0
TreeFamiTF314180

Family and domain databases

Gene3Di1.10.10.1050, 1 hit
InterProiView protein in InterPro
IPR007722 DCP2_BoxA
IPR036189 DCP2_BoxA_sf
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF05026 DCP2, 1 hit
PF00293 NUDIX, 1 hit
SMARTiView protein in SMART
SM01125 DCP2, 1 hit
SUPFAMiSSF140586 SSF140586, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9CYC6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEPKRLEIPG SVLDDLCSRF ILHIPSEERD NAIRVCFQIE LAHWFYLDFY
60 70 80 90 100
MQNTPGLPQC GIRDFAKAVF SHCPFLLPQG EDVEKILDEW KEYKMGVPTY
110 120 130 140 150
GAIILDETLE NVLLVQGYLA KSGWGFPKGK VNKEEAPHDC AAREVFEETG
160 170 180 190 200
FDIKDYICKD DYIELRINDQ LARLYIIPGV PKDTKFNPKT RREIRNIEWF
210 220 230 240 250
SIEKLPCHRN DMTPKSKLGL APNKFFMAIP FIRPLRDWLS RRFGDSSDSD
260 270 280 290 300
NGFSSAGSTP ARPTVEKLSR TKFRHSQQLF PEGSPSDQWV KHRQPLQQKS
310 320 330 340 350
HSNHGEVSDL LKAKNQNMRG NGRKQYQDSP NQKKRANGVH GQPAKQQNPL
360 370 380 390 400
VKCEKKLHPR KLQDNFETDA TCDLPCSGEE PSVEHAEGHS VACNGHCKFP
410 420
FSSRAFLSFK FDQNAIMKIL DL
Length:422
Mass (Da):48,380
Last modified:July 27, 2011 - v2
Checksum:i80FE791B05E4C8FD
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0J9YU54A0A0J9YU54_MOUSE
m7GpppN-mRNA hydrolase
Dcp2
82Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti226F → Y in BAB30946 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017809 mRNA Translation: BAB30946.1
AK166560 mRNA Translation: BAE38853.1
CCDSiCCDS50275.1
RefSeqiNP_081766.1, NM_027490.1
UniGeneiMm.87629

Genome annotation databases

EnsembliENSMUST00000025350; ENSMUSP00000025350; ENSMUSG00000024472
GeneIDi70640
KEGGimmu:70640
UCSCiuc008eux.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017809 mRNA Translation: BAB30946.1
AK166560 mRNA Translation: BAE38853.1
CCDSiCCDS50275.1
RefSeqiNP_081766.1, NM_027490.1
UniGeneiMm.87629

3D structure databases

ProteinModelPortaliQ9CYC6
SMRiQ9CYC6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000025350

PTM databases

iPTMnetiQ9CYC6
PhosphoSitePlusiQ9CYC6

Proteomic databases

EPDiQ9CYC6
MaxQBiQ9CYC6
PaxDbiQ9CYC6
PRIDEiQ9CYC6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000025350; ENSMUSP00000025350; ENSMUSG00000024472
GeneIDi70640
KEGGimmu:70640
UCSCiuc008eux.2 mouse

Organism-specific databases

CTDi167227
MGIiMGI:1917890 Dcp2

Phylogenomic databases

eggNOGiKOG2937 Eukaryota
COG0494 LUCA
GeneTreeiENSGT00390000018878
HOGENOMiHOG000005974
HOVERGENiHBG051321
InParanoidiQ9CYC6
KOiK12613
OMAiVHSQPAK
OrthoDBiEOG091G05Q0
TreeFamiTF314180

Enzyme and pathway databases

BRENDAi3.6.1.62 3474
ReactomeiR-MMU-430039 mRNA decay by 5' to 3' exoribonuclease
R-MMU-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-MMU-450604 KSRP (KHSRP) binds and destabilizes mRNA

Miscellaneous databases

PROiPR:Q9CYC6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024472 Expressed in 238 organ(s), highest expression level in blastocyst
CleanExiMM_DCP2
ExpressionAtlasiQ9CYC6 baseline and differential
GenevisibleiQ9CYC6 MM

Family and domain databases

Gene3Di1.10.10.1050, 1 hit
InterProiView protein in InterPro
IPR007722 DCP2_BoxA
IPR036189 DCP2_BoxA_sf
IPR015797 NUDIX_hydrolase-like_dom_sf
IPR020084 NUDIX_hydrolase_CS
IPR000086 NUDIX_hydrolase_dom
PfamiView protein in Pfam
PF05026 DCP2, 1 hit
PF00293 NUDIX, 1 hit
SMARTiView protein in SMART
SM01125 DCP2, 1 hit
SUPFAMiSSF140586 SSF140586, 1 hit
SSF55811 SSF55811, 1 hit
PROSITEiView protein in PROSITE
PS51462 NUDIX, 1 hit
PS00893 NUDIX_BOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDCP2_MOUSE
AccessioniPrimary (citable) accession number: Q9CYC6
Secondary accession number(s): Q3TLD9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 1, 2005
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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