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Protein

Zinc finger CCHC domain-containing protein 8

Gene

Zcchc8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Scaffolding subunit of the trimeric nuclear exosome targeting (NEXT) complex, a complex that directs a subset of non-coding short-lived RNAs for exosomal degradation. The RNA exosome is fundamental for the degradation of RNA in eukaryotic nuclei. Substrate targeting is facilitated by its cofactor MTREX, which links to RNA-binding protein adapters. May be involved in pre-mRNA splicing.By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri230 – 247CCHC-typePROSITE-ProRule annotationAdd BLAST18

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processmRNA processing, mRNA splicing
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
Zinc finger CCHC domain-containing protein 8
Alternative name(s):
TRAMP-like complex RNA-binding factor ZCCHC8
Gene namesi
Name:Zcchc8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1917900 Zcchc8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus, Spliceosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001509612 – 709Zinc finger CCHC domain-containing protein 8Add BLAST708

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei474PhosphothreonineBy similarity1
Modified residuei482PhosphothreonineBy similarity1
Modified residuei488PhosphothreonineBy similarity1
Modified residuei495PhosphothreonineBy similarity1
Modified residuei601PhosphoserineBy similarity1
Modified residuei660PhosphoserineBy similarity1
Modified residuei697PhosphoserineBy similarity1

Post-translational modificationi

Phosphorylation at Thr-495 by GSK3 is triggered in cells entering mitosis.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CYA6
MaxQBiQ9CYA6
PaxDbiQ9CYA6
PRIDEiQ9CYA6

PTM databases

iPTMnetiQ9CYA6
PhosphoSitePlusiQ9CYA6

Miscellaneous databases

PMAP-CutDBiQ9CYA6

Expressioni

Gene expression databases

BgeeiENSMUSG00000029427 Expressed in 274 organ(s), highest expression level in primary oocyte
CleanExiMM_ZCCHC8
ExpressionAtlasiQ9CYA6 baseline and differential
GenevisibleiQ9CYA6 MM

Interactioni

Subunit structurei

Component of a nuclear TRAMP-like complex, an ATP-dependent exosome regulatory complex consisting of a helicase (MTREX), an oligadenylate polymerase (TENT4B or TENT4A), and a substrate specific RNA-binding factor (ZCCHC7 or ZCCHC8). Several TRAMP-like complexes exist with specific compositions and are associated with nuclear, or nucleolar RNA exosomes. Identified in the spliceosome C complex. Component of the nuclear exosome targeting (NEXT) complex composed of MTREX, ZCCHC8, and RBM7 that directs a subset of non-coding short-lived RNAs for exosomal degradation. Interacts with proteins involved in RNA processing and degradation such as MTREX and RBM7.By similarity

Protein-protein interaction databases

BioGridi214189, 6 interactors
IntActiQ9CYA6, 4 interactors
MINTiQ9CYA6
STRINGi10090.ENSMUSP00000031376

Structurei

3D structure databases

SMRiQ9CYA6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili48 – 83Sequence analysisAdd BLAST36

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi35 – 38Poly-Glu4
Compositional biasi39 – 43Poly-Asp5
Compositional biasi468 – 508Pro-richAdd BLAST41

Sequence similaritiesi

Belongs to the ZCCHC8 family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri230 – 247CCHC-typePROSITE-ProRule annotationAdd BLAST18

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2673 Eukaryota
ENOG410XPDY LUCA
GeneTreeiENSGT00390000011475
HOGENOMiHOG000155769
HOVERGENiHBG079371
InParanoidiQ9CYA6
KOiK13128
OMAiWLKEAEM
OrthoDBiEOG091G0KC3
PhylomeDBiQ9CYA6
TreeFamiTF321837

Family and domain databases

InterProiView protein in InterPro
IPR006568 PSP_pro-rich
IPR001878 Znf_CCHC
PfamiView protein in Pfam
PF04046 PSP, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00581 PSP, 1 hit
SM00343 ZnF_C2HC, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All

Q9CYA6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGVDFGDL ELFEAFDPPE ESTPKPVHTR FKDDEEEEDD DDDENGVGDA
60 70 80 90 100
ELQEQLRRCE ATIEQLRAEN QELKRKLNIL TRPSGILVSN TKIDGPLLQI
110 120 130 140 150
LFMNNAISKQ YHQEIEEFVS NLVKRFEEQQ KNDVEKTSFS LLPQPSSVML
160 170 180 190 200
EEDHKVEESC AVKNNKEAFS VVGSVLYFTN FCLDKLGQPL LNENPQLTEG
210 220 230 240 250
WEIPKYQQVF SHIVPLEGQE MQVKAKRPKP HCFNCGSEEH QMKECPMPRN
260 270 280 290 300
AARISEKRKE YMDACGEASG QSFQQRYHAE EVEERFGRFK PGVISEELQD
310 320 330 340 350
ALGVTDKSLP PFIYRMRQLG YPPGWLKEAE LENSGLALYD GNDDADGETE
360 370 380 390 400
TGEIQNKNVT YDLSKLVNYP GFNISTPRGI PDEWRMFGSI PMQACQQKDV
410 420 430 440 450
FASYLNSNIQ SPSMRSSGKR SSSQSSPNSP KKQRKEGSAA ASPADMELDS
460 470 480 490 500
DVEIPPGSQS SKAFQFQPPL PPGTPPPLPQ GTPPPLFTPP LPKGTPPLTP
510 520 530 540 550
SDSPQARPAA SAMDEDALTL EELEEQQRQI WAALQQAEGG NGDSDVPGDT
560 570 580 590 600
PLTGNSVASS PCPNEFDLPV PEGKALEKPV LAEPQEPAAS VDTAGPEPSC
610 620 630 640 650
SPAAGAAVLS QREEEAAAEG GPGDALLDNG GVLNMNMSNG SNQQPIHPDS
660 670 680 690 700
RPPMAPKTHS PVPDMSKFAT GITPFEFENM AESTGMYLRI RNLLKNSPRN

QQKNKKTCE
Length:709
Mass (Da):78,026
Last modified:May 10, 2005 - v3
Checksum:iBDBB3EEF1C6A74F4
GO

Computationally mapped potential isoform sequencesi

There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JGW3A0A0G2JGW3_MOUSE
Zinc finger CCHC domain-containing ...
Zcchc8
103Annotation score:
A0A0G2JDI6A0A0G2JDI6_MOUSE
Zinc finger CCHC domain-containing ...
Zcchc8
107Annotation score:
A0A0G2JFE4A0A0G2JFE4_MOUSE
Zinc finger CCHC domain-containing ...
Zcchc8
173Annotation score:
A0A0G2JH09A0A0G2JH09_MOUSE
Zinc finger CCHC domain-containing ...
Zcchc8
105Annotation score:
A0A0G2JDW3A0A0G2JDW3_MOUSE
Zinc finger CCHC domain-containing ...
Zcchc8
54Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti2A → G in BAC28304 (PubMed:16141072).Curated1
Sequence conflicti11E → K in BAC28304 (PubMed:16141072).Curated1
Sequence conflicti330E → G in BAC28304 (PubMed:16141072).Curated1
Sequence conflicti659H → R in BAC28304 (PubMed:16141072).Curated1
Sequence conflicti695K → E in BAB30977 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017857 mRNA Translation: BAB30977.2
AK033469 mRNA Translation: BAC28304.1
AK156770 mRNA Translation: BAE33847.1
AK167126 mRNA Translation: BAE39274.1
AK167736 mRNA Translation: BAE39776.1
BC013555 mRNA Translation: AAH13555.1
CCDSiCCDS80406.1
RefSeqiNP_081770.3, NM_027494.3
UniGeneiMm.279427

Genome annotation databases

EnsembliENSMUST00000031376; ENSMUSP00000031376; ENSMUSG00000029427
ENSMUST00000196282; ENSMUSP00000142363; ENSMUSG00000029427
GeneIDi70650
KEGGimmu:70650
UCSCiuc008zoh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK017857 mRNA Translation: BAB30977.2
AK033469 mRNA Translation: BAC28304.1
AK156770 mRNA Translation: BAE33847.1
AK167126 mRNA Translation: BAE39274.1
AK167736 mRNA Translation: BAE39776.1
BC013555 mRNA Translation: AAH13555.1
CCDSiCCDS80406.1
RefSeqiNP_081770.3, NM_027494.3
UniGeneiMm.279427

3D structure databases

SMRiQ9CYA6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi214189, 6 interactors
IntActiQ9CYA6, 4 interactors
MINTiQ9CYA6
STRINGi10090.ENSMUSP00000031376

PTM databases

iPTMnetiQ9CYA6
PhosphoSitePlusiQ9CYA6

Proteomic databases

EPDiQ9CYA6
MaxQBiQ9CYA6
PaxDbiQ9CYA6
PRIDEiQ9CYA6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000031376; ENSMUSP00000031376; ENSMUSG00000029427
ENSMUST00000196282; ENSMUSP00000142363; ENSMUSG00000029427
GeneIDi70650
KEGGimmu:70650
UCSCiuc008zoh.2 mouse

Organism-specific databases

CTDi55596
MGIiMGI:1917900 Zcchc8

Phylogenomic databases

eggNOGiKOG2673 Eukaryota
ENOG410XPDY LUCA
GeneTreeiENSGT00390000011475
HOGENOMiHOG000155769
HOVERGENiHBG079371
InParanoidiQ9CYA6
KOiK13128
OMAiWLKEAEM
OrthoDBiEOG091G0KC3
PhylomeDBiQ9CYA6
TreeFamiTF321837

Miscellaneous databases

PMAP-CutDBiQ9CYA6
PROiPR:Q9CYA6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000029427 Expressed in 274 organ(s), highest expression level in primary oocyte
CleanExiMM_ZCCHC8
ExpressionAtlasiQ9CYA6 baseline and differential
GenevisibleiQ9CYA6 MM

Family and domain databases

InterProiView protein in InterPro
IPR006568 PSP_pro-rich
IPR001878 Znf_CCHC
PfamiView protein in Pfam
PF04046 PSP, 1 hit
PF00098 zf-CCHC, 1 hit
SMARTiView protein in SMART
SM00581 PSP, 1 hit
SM00343 ZnF_C2HC, 1 hit
PROSITEiView protein in PROSITE
PS50158 ZF_CCHC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiZCHC8_MOUSE
AccessioniPrimary (citable) accession number: Q9CYA6
Secondary accession number(s): Q3TK71, Q8CCB7, Q91WQ1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: May 10, 2005
Last modified: November 7, 2018
This is version 125 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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