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Protein

Protein MIS12 homolog

Gene

Mis12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.By similarity

GO - Biological processi

Keywordsi

Biological processCell cycle, Cell division, Chromosome partition, Mitosis

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Names & Taxonomyi

Protein namesi
Recommended name:
Protein MIS12 homolog
Gene namesi
Name:Mis12Imported
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1914389 Mis12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Centromere, Chromosome, Kinetochore

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002482351 – 206Protein MIS12 homologAdd BLAST206

Proteomic databases

EPDiQ9CY25
MaxQBiQ9CY25
PaxDbiQ9CY25
PeptideAtlasiQ9CY25
PRIDEiQ9CY25

PTM databases

iPTMnetiQ9CY25
PhosphoSitePlusiQ9CY25

Expressioni

Gene expression databases

BgeeiENSMUSG00000040599 Expressed in 271 organ(s), highest expression level in cochlea
CleanExiMM_MIS12
ExpressionAtlasiQ9CY25 baseline and differential
GenevisibleiQ9CY25 MM

Interactioni

Subunit structurei

Component of the MIS12 complex composed of MIS12, DSN1, NSL1 and PMF1. Also interacts with KNL1, CBX3, CBX5, NDC80 and ZWINT.By similarity

Protein-protein interaction databases

BioGridi211970, 84 interactors
IntActiQ9CY25, 87 interactors
MINTiQ9CY25
STRINGi10090.ENSMUSP00000039500

Structurei

3D structure databases

SMRiQ9CY25
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili102 – 206Sequence analysisAdd BLAST105

Sequence similaritiesi

Belongs to the mis12 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IQVW Eukaryota
ENOG4111HUU LUCA
GeneTreeiENSGT00390000018665
HOGENOMiHOG000251597
HOVERGENiHBG055269
InParanoidiQ9CY25
KOiK11543
OMAiDKSQEMH
OrthoDBiEOG091G0O0P
PhylomeDBiQ9CY25
TreeFamiTF101136

Family and domain databases

InterProiView protein in InterPro
IPR008685 Centromere_Mis12
PANTHERiPTHR14527 PTHR14527, 1 hit
PfamiView protein in Pfam
PF05859 Mis12, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9CY25-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSVDPMAYEA QFFGFTPQTC LLRIYVAFQD HLFEVMQAVE QVILKKLEDI
60 70 80 90 100
PNCEITPVQT RKCTEKFLCF MKGRFDNLFG KMEQLILQSI LCIPPNILLP
110 120 130 140 150
EDKCQETNPF SEEKLELLQQ EIKELQEKYK VELCTEQALL AELEEQKTVK
160 170 180 190 200
AKLRETLTFF DELENIGRYQ GTSNFRESLA SLVQSCRKLQ SIRDNVEKES

RRLETQ
Length:206
Mass (Da):24,131
Last modified:June 1, 2001 - v1
Checksum:i3492940A9EC23254
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A3QK65A3QK65_MOUSE
Protein MIS12 homolog
Mis12
12Annotation score:
A3QK66A3QK66_MOUSE
Protein MIS12 homolog
Mis12
17Annotation score:
A0A0G2JDA2A0A0G2JDA2_MOUSE
Protein MIS12 homolog
Mis12
8Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti161D → N in BAE20937 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010995 mRNA Translation: BAB27314.1
AK132010 mRNA Translation: BAE20937.1
AK139957 mRNA Translation: BAE24195.1
AK154531 mRNA Translation: BAE32656.1
AL596136 Genomic DNA No translation available.
BC026790 mRNA Translation: AAH26790.1
CCDSiCCDS24973.1
RefSeqiNP_080269.1, NM_025993.2
XP_006534031.1, XM_006533968.2
XP_006534032.1, XM_006533969.1
UniGeneiMm.28227

Genome annotation databases

EnsembliENSMUST00000048807; ENSMUSP00000039500; ENSMUSG00000040599
ENSMUST00000164220; ENSMUSP00000127782; ENSMUSG00000040599
GeneIDi67139
KEGGimmu:67139
UCSCiuc007jxm.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010995 mRNA Translation: BAB27314.1
AK132010 mRNA Translation: BAE20937.1
AK139957 mRNA Translation: BAE24195.1
AK154531 mRNA Translation: BAE32656.1
AL596136 Genomic DNA No translation available.
BC026790 mRNA Translation: AAH26790.1
CCDSiCCDS24973.1
RefSeqiNP_080269.1, NM_025993.2
XP_006534031.1, XM_006533968.2
XP_006534032.1, XM_006533969.1
UniGeneiMm.28227

3D structure databases

SMRiQ9CY25
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211970, 84 interactors
IntActiQ9CY25, 87 interactors
MINTiQ9CY25
STRINGi10090.ENSMUSP00000039500

PTM databases

iPTMnetiQ9CY25
PhosphoSitePlusiQ9CY25

Proteomic databases

EPDiQ9CY25
MaxQBiQ9CY25
PaxDbiQ9CY25
PeptideAtlasiQ9CY25
PRIDEiQ9CY25

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000048807; ENSMUSP00000039500; ENSMUSG00000040599
ENSMUST00000164220; ENSMUSP00000127782; ENSMUSG00000040599
GeneIDi67139
KEGGimmu:67139
UCSCiuc007jxm.1 mouse

Organism-specific databases

CTDi79003
MGIiMGI:1914389 Mis12

Phylogenomic databases

eggNOGiENOG410IQVW Eukaryota
ENOG4111HUU LUCA
GeneTreeiENSGT00390000018665
HOGENOMiHOG000251597
HOVERGENiHBG055269
InParanoidiQ9CY25
KOiK11543
OMAiDKSQEMH
OrthoDBiEOG091G0O0P
PhylomeDBiQ9CY25
TreeFamiTF101136

Enzyme and pathway databases

ReactomeiR-MMU-141444 Amplification of signal from unattached kinetochores via a MAD2 inhibitory signal
R-MMU-2467813 Separation of Sister Chromatids
R-MMU-2500257 Resolution of Sister Chromatid Cohesion
R-MMU-5663220 RHO GTPases Activate Formins
R-MMU-68877 Mitotic Prometaphase

Miscellaneous databases

PROiPR:Q9CY25
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040599 Expressed in 271 organ(s), highest expression level in cochlea
CleanExiMM_MIS12
ExpressionAtlasiQ9CY25 baseline and differential
GenevisibleiQ9CY25 MM

Family and domain databases

InterProiView protein in InterPro
IPR008685 Centromere_Mis12
PANTHERiPTHR14527 PTHR14527, 1 hit
PfamiView protein in Pfam
PF05859 Mis12, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMIS12_MOUSE
AccessioniPrimary (citable) accession number: Q9CY25
Secondary accession number(s): Q3V261
, Q5QNU7, Q5QNU8, Q5QNU9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 5, 2006
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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