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Protein

Mediator of RNA polymerase II transcription subunit 10

Gene

Med10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

  • positive regulation of transcription by RNA polymerase II Source: MGI
  • protein ubiquitination Source: MGI
  • stem cell population maintenance Source: MGI

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 10
Alternative name(s):
Mediator complex subunit 10
Gene namesi
Name:Med10
Synonyms:D13Wsu50e
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:106331 Med10

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003031531 – 135Mediator of RNA polymerase II transcription subunit 10Add BLAST135

Proteomic databases

EPDiQ9CXU0
MaxQBiQ9CXU0
PaxDbiQ9CXU0
PeptideAtlasiQ9CXU0
PRIDEiQ9CXU0

PTM databases

iPTMnetiQ9CXU0
PhosphoSitePlusiQ9CXU0

Expressioni

Gene expression databases

BgeeiENSMUSG00000021598 Expressed in 285 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiQ9CXU0 baseline and differential
GenevisibleiQ9CXU0 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Protein-protein interaction databases

ComplexPortaliCPX-3264 Core mediator complex
IntActiQ9CXU0, 2 interactors
STRINGi10090.ENSMUSP00000022089

Structurei

3D structure databases

SMRiQ9CXU0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the Mediator complex subunit 10 family.Curated

Phylogenomic databases

eggNOGiKOG3046 Eukaryota
ENOG4111QA2 LUCA
GeneTreeiENSGT00390000014501
HOGENOMiHOG000006789
HOVERGENiHBG107408
InParanoidiQ9CXU0
KOiK15151
OMAiRNEQVKG
OrthoDBiEOG091G0Y09
PhylomeDBiQ9CXU0
TreeFamiTF315096

Family and domain databases

InterProiView protein in InterPro
IPR019145 Mediator_Med10
PfamiView protein in Pfam
PF09748 Med10, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9CXU0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAEKFDHLEE HLEKFVENIR QLGIIVSDFQ PSSQAGLSQK LNFIVTGLQD
60 70 80 90 100
IDKCRQQLHD ITVPLEVFEY IDQGRNPQLY TKECLERALA KNEQVKGKID
110 120 130
TMKKFKSLLI QELSKVFPED MAKYRSIRGE DHPPS
Length:135
Mass (Da):15,661
Last modified:June 1, 2001 - v1
Checksum:i61143396990FA91F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1Y7VMY2A0A1Y7VMY2_MOUSE
Mediator of RNA polymerase II trans...
Med10 MED10
154Annotation score:
A0A1Y7VKE4A0A1Y7VKE4_MOUSE
Mediator of RNA polymerase II trans...
Med10 MED10
100Annotation score:
A0A1Y7VKC0A0A1Y7VKC0_MOUSE
Mediator of RNA polymerase II trans...
Med10 MED10
83Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti130E → G in BAE33733 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013992 mRNA Translation: BAB29103.1
AK156498 mRNA Translation: BAE33733.1
BC048638 mRNA Translation: AAH48638.2
BC056438 mRNA Translation: AAH56438.1
CCDSiCCDS26625.1
RefSeqiNP_613062.1, NM_138596.2
UniGeneiMm.491490
Mm.52583

Genome annotation databases

EnsembliENSMUST00000022089; ENSMUSP00000022089; ENSMUSG00000021598
GeneIDi28077
KEGGimmu:28077
UCSCiuc007rcq.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK013992 mRNA Translation: BAB29103.1
AK156498 mRNA Translation: BAE33733.1
BC048638 mRNA Translation: AAH48638.2
BC056438 mRNA Translation: AAH56438.1
CCDSiCCDS26625.1
RefSeqiNP_613062.1, NM_138596.2
UniGeneiMm.491490
Mm.52583

3D structure databases

SMRiQ9CXU0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-3264 Core mediator complex
IntActiQ9CXU0, 2 interactors
STRINGi10090.ENSMUSP00000022089

PTM databases

iPTMnetiQ9CXU0
PhosphoSitePlusiQ9CXU0

Proteomic databases

EPDiQ9CXU0
MaxQBiQ9CXU0
PaxDbiQ9CXU0
PeptideAtlasiQ9CXU0
PRIDEiQ9CXU0

Protocols and materials databases

DNASUi28077
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022089; ENSMUSP00000022089; ENSMUSG00000021598
GeneIDi28077
KEGGimmu:28077
UCSCiuc007rcq.1 mouse

Organism-specific databases

CTDi84246
MGIiMGI:106331 Med10

Phylogenomic databases

eggNOGiKOG3046 Eukaryota
ENOG4111QA2 LUCA
GeneTreeiENSGT00390000014501
HOGENOMiHOG000006789
HOVERGENiHBG107408
InParanoidiQ9CXU0
KOiK15151
OMAiRNEQVKG
OrthoDBiEOG091G0Y09
PhylomeDBiQ9CXU0
TreeFamiTF315096

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Miscellaneous databases

PROiPR:Q9CXU0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021598 Expressed in 285 organ(s), highest expression level in secondary oocyte
ExpressionAtlasiQ9CXU0 baseline and differential
GenevisibleiQ9CXU0 MM

Family and domain databases

InterProiView protein in InterPro
IPR019145 Mediator_Med10
PfamiView protein in Pfam
PF09748 Med10, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED10_MOUSE
AccessioniPrimary (citable) accession number: Q9CXU0
Secondary accession number(s): Q3U0W8, Q80VV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 116 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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