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Protein

JmjC domain-containing protein 5

Gene

Kdm8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Histone demethylase required for G2/M phase cell cycle progression. Specifically demethylates dimethylated 'Lys-36' (H3K36me2) of histone H3, an epigenetic repressive mark, thereby acting as a transcription activator. Regulates expression of CCNA1 (cyclin-A1), leading to regulate cancer cell proliferation. In a complex with coregulator RCCD1, is also able to demethylate trimethylated 'Lys-36' (H3K36me3) of histone H3. Plays a role in transcriptional repression of satellite repeats, possibly by regulating H3K36 methylation levels in centromeric regions together with RCCD1. Possibly together with RCCD1, involved in proper mitotic spindle organization and chromosome segregation. Plays a role in regulating alpha-tubulin acetylation and cytoskeletal microtubule stability (By similarity). Might function as a protein hydroxylase (Potential). Under stress conditions that cause a DNA damage response, acts as a histone protease by cleaving the N-terminal tail of histone H3 at the carboxyl side of monomethyl-lysine (Kme1) residues, preferably at monomethylated 'Lys-9' (H3K9me1). The histone variant H3F3A is the major target for cleavage.By similarity

Caution

The demethylase activity of JMJD5 is controversial. Demethylase activity towards H3K36me2 was observed in vivo and in vitro. In addition, demethylase activity towards H3K36me3 when in a complex with RCCD1 has been observed. In contrast, in other studies, JMJD5 was shown not to display any demethylase activity toward methylated H3K36 nor toward other methyllysines in the N-terminal tails of H3 and H4 in vitro. It has been suggested that JMJD5 might function as a protein hydroxylase instead.By similarity

Catalytic activityi

Protein N6,N6-dimethyl-L-lysine + 2 2-oxoglutarate + 2 O2 = protein L-lysine + 2 succinate + 2 formaldehyde + 2 CO2.

Cofactori

Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei2702-oxoglutarateBy similarity1
Binding sitei3162-oxoglutarateBy similarity1
Metal bindingi319Iron; catalyticPROSITE-ProRule annotation1
Metal bindingi321Iron; catalyticPROSITE-ProRule annotation1
Binding sitei3252-oxoglutarateBy similarity1
Binding sitei3342-oxoglutarateBy similarity1
Metal bindingi398Iron; catalyticPROSITE-ProRule annotation1
Binding sitei4122-oxoglutarateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionChromatin regulator, Dioxygenase, Hydrolase, Oxidoreductase, Protease
Biological processCell cycle, Transcription, Transcription regulation
LigandIron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-3214842 HDMs demethylate histones

Names & Taxonomyi

Protein namesi
Recommended name:
JmjC domain-containing protein 5By similarity (EC:1.14.11.27, EC:3.4.-.-)
Alternative name(s):
Jumonji domain-containing protein 5
Lysine-specific demethylase 8
Gene namesi
Name:Kdm8
Synonyms:Jmjd5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1924285 Kdm8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chromosome, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002920111 – 414JmjC domain-containing protein 5Add BLAST414

Proteomic databases

MaxQBiQ9CXT6
PaxDbiQ9CXT6
PRIDEiQ9CXT6

PTM databases

iPTMnetiQ9CXT6
PhosphoSitePlusiQ9CXT6

Expressioni

Gene expression databases

BgeeiENSMUSG00000030752 Expressed in 219 organ(s), highest expression level in cumulus cell
CleanExiMM_JMJD5
ExpressionAtlasiQ9CXT6 baseline and differential
GenevisibleiQ9CXT6 MM

Interactioni

Subunit structurei

Can form homodimers (via JmjC domain). Found in a complex with RCCD1. Interacts (via N-terminus) with RCCD1 (via N-terminus); this interaction stimulates H3K36me3 and H3K36me2 demethylation. Interacts (via JmjC domain) with HIST1H3A.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033010

Structurei

3D structure databases

ProteinModelPortaliQ9CXT6
SMRiQ9CXT6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini269 – 414JmjCPROSITE-ProRule annotationAdd BLAST146

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 107Interaction with RCCD1By similarityAdd BLAST107

Phylogenomic databases

eggNOGiKOG2132 Eukaryota
ENOG410XQDR LUCA
GeneTreeiENSGT00530000062914
HOVERGENiHBG105493
InParanoidiQ9CXT6
KOiK10277
OMAiRVYAFGC
OrthoDBiEOG091G069K
PhylomeDBiQ9CXT6
TreeFamiTF315056

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9CXT6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEDTTEPLV GSSTLWKELR TLLPDKEEEL KLDLGEKVDR SVAALLRQAV
60 70 80 90 100
GLFYAGHWQG CLQASEAVLD YSWEKLNTGP WRDVDKEWRR VYSFGCLLKA
110 120 130 140 150
LCLCQAPQKA TTVVEALRVC DMGLLMGAAI LEDILLKVVA VLQTHQLPGK
160 170 180 190 200
QPARGPHQDQ PATKKAKCDA SPAPDVMLER MVPRLRCPPL QYFKQHFLVP
210 220 230 240 250
GRPVILEGVA DHWPCMKKWS LQYIQEIAGC RTVPVEVGSR YTDEDWSQTL
260 270 280 290 300
MTVDEFIQKF ILSEAKDVGY LAQHQLFDQI PELKRDISIP DYCCLGNGEE
310 320 330 340 350
EEITINAWFG PQGTISPLHQ DPQQNFLVQV LGRKYIRLYS PQESEAVYPH
360 370 380 390 400
ETHILHNTSQ VDVENPDLEK FPKFTEAPFL SCILSPGDTL FIPAKYWHYV
410
RSLDLSFSVS FWWS
Length:414
Mass (Da):47,145
Last modified:June 1, 2001 - v1
Checksum:i4C794D0F5E9EC996
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YUV8D3YUV8_MOUSE
JmjC domain-containing protein 5
Kdm8 Jmjd5
152Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014006 mRNA Translation: BAB29111.1
BC024807 mRNA Translation: AAH24807.1
CCDSiCCDS21821.1
RefSeqiNP_084118.1, NM_029842.5
UniGeneiMm.307239

Genome annotation databases

EnsembliENSMUST00000033010; ENSMUSP00000033010; ENSMUSG00000030752
GeneIDi77035
KEGGimmu:77035
UCSCiuc009jpz.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK014006 mRNA Translation: BAB29111.1
BC024807 mRNA Translation: AAH24807.1
CCDSiCCDS21821.1
RefSeqiNP_084118.1, NM_029842.5
UniGeneiMm.307239

3D structure databases

ProteinModelPortaliQ9CXT6
SMRiQ9CXT6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000033010

PTM databases

iPTMnetiQ9CXT6
PhosphoSitePlusiQ9CXT6

Proteomic databases

MaxQBiQ9CXT6
PaxDbiQ9CXT6
PRIDEiQ9CXT6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033010; ENSMUSP00000033010; ENSMUSG00000030752
GeneIDi77035
KEGGimmu:77035
UCSCiuc009jpz.2 mouse

Organism-specific databases

CTDi79831
MGIiMGI:1924285 Kdm8

Phylogenomic databases

eggNOGiKOG2132 Eukaryota
ENOG410XQDR LUCA
GeneTreeiENSGT00530000062914
HOVERGENiHBG105493
InParanoidiQ9CXT6
KOiK10277
OMAiRVYAFGC
OrthoDBiEOG091G069K
PhylomeDBiQ9CXT6
TreeFamiTF315056

Enzyme and pathway databases

ReactomeiR-MMU-3214842 HDMs demethylate histones

Miscellaneous databases

PROiPR:Q9CXT6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000030752 Expressed in 219 organ(s), highest expression level in cumulus cell
CleanExiMM_JMJD5
ExpressionAtlasiQ9CXT6 baseline and differential
GenevisibleiQ9CXT6 MM

Family and domain databases

InterProiView protein in InterPro
IPR003347 JmjC_dom
SMARTiView protein in SMART
SM00558 JmjC, 1 hit
PROSITEiView protein in PROSITE
PS51184 JMJC, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiKDM8_MOUSE
AccessioniPrimary (citable) accession number: Q9CXT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 26, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 106 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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