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Protein

E3 ubiquitin-protein ligase RNF135

Gene

Rnf135

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Acts as an E2-dependent E3 ubiquitin-protein ligase, involved in innate immune defense against viruses. Ubiquitinates DDX58 and is required for full activation of the DDX58 signaling resulting in interferon beta production.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 67RING-typePROSITE-ProRule annotationAdd BLAST47

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionReceptor, Transferase
Biological processImmunity, Innate immunity, Ubl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase RNF135 (EC:2.3.2.27)
Alternative name(s):
RING finger protein 135
RING-type E3 ubiquitin transferase RNF135Curated
Gene namesi
Name:Rnf135
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:1919206 Rnf135

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002805581 – 417E3 ubiquitin-protein ligase RNF135Add BLAST417

Proteomic databases

EPDiQ9CWS1
PaxDbiQ9CWS1
PeptideAtlasiQ9CWS1
PRIDEiQ9CWS1

PTM databases

iPTMnetiQ9CWS1
PhosphoSitePlusiQ9CWS1

Expressioni

Gene expression databases

BgeeiENSMUSG00000020707 Expressed in 262 organ(s), highest expression level in liver
CleanExiMM_RNF135
ExpressionAtlasiQ9CWS1 baseline and differential
GenevisibleiQ9CWS1 MM

Interactioni

Subunit structurei

Interacts with DDX58. Interacts with PCBP2.By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9CWS1, 1 interactor
MINTiQ9CWS1
STRINGi10090.ENSMUSP00000017839

Structurei

3D structure databases

ProteinModelPortaliQ9CWS1
SMRiQ9CWS1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini225 – 417B30.2/SPRYPROSITE-ProRule annotationAdd BLAST193

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili121 – 145Sequence analysisAdd BLAST25
Coiled coili180 – 204Sequence analysisAdd BLAST25

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri21 – 67RING-typePROSITE-ProRule annotationAdd BLAST47

Keywords - Domaini

Coiled coil, Zinc-finger

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00830000128449
HOGENOMiHOG000060145
HOVERGENiHBG056606
InParanoidiQ9CWS1
KOiK16272
OMAiNCSHWAV
OrthoDBiEOG091G062V
PhylomeDBiQ9CWS1
TreeFamiTF351089

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR003877 SPRY_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00622 SPRY, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9CWS1-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAAVCSGNAV PVWLSEDDLS CIICQGLLDQ PTTLPCGHSF CLRCLHDLWV
60 70 80 90 100
SKRGAVDGCP WACPICRKGP LTKPKLHKNP LLQDLVDKYL QAAREVEAGS
110 120 130 140 150
EPEPAPAPRS APQVTVQKST TNVIQELTDM VRQLVDDVKS LQTQRPNLGS
160 170 180 190 200
GQDNAQGTPP TDSSSEGEHS LDSPKLVTFS ISQKKIQEIL HNLEEIQEKL
210 220 230 240 250
QGSVPGRAPP RERVQEMTSS LCLLPDQRRP APRKASHLSL WAISPTFDLR
260 270 280 290 300
TLSYNLEVSN NSRRVTVSRG DLHTYHWSPQ RFSISQVFCS QALSSGQKYW
310 320 330 340 350
EVDTRNCSHW AIGVASWGMK RDGMLGRTMD SWCIEWRGPG QFSAWAKMKK
360 370 380 390 400
TDLQSDLPEV VGVWLDLESG ELAFYAVADH ERLLYECEVS SSSPLHPAFW
410
LYGLSPGNYL EIKQLNT
Length:417
Mass (Da):46,462
Last modified:June 1, 2001 - v1
Checksum:iC79AD6FA1D2CF683
GO
Isoform 2 (identifier: Q9CWS1-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     78-346: Missing.

Note: No experimental confirmation available.
Show »
Length:148
Mass (Da):16,359
Checksum:iF095E746C20B38E9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_02378778 – 346Missing in isoform 2. 1 PublicationAdd BLAST269

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002781 mRNA Translation: BAB22354.1
AK010429 mRNA Translation: BAB26931.1
AK150921 mRNA Translation: BAE29959.1
AK152096 mRNA Translation: BAE30944.1
AL591426 Genomic DNA No translation available.
BC025209 mRNA Translation: AAH25209.1
BC096385 mRNA Translation: AAH96385.1
BC132307 mRNA Translation: AAI32308.1
CCDSiCCDS25129.1 [Q9CWS1-1]
RefSeqiNP_082295.1, NM_028019.3 [Q9CWS1-1]
UniGeneiMm.22985

Genome annotation databases

EnsembliENSMUST00000017839; ENSMUSP00000017839; ENSMUSG00000020707 [Q9CWS1-1]
GeneIDi71956
KEGGimmu:71956
UCSCiuc007kln.1 mouse [Q9CWS1-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002781 mRNA Translation: BAB22354.1
AK010429 mRNA Translation: BAB26931.1
AK150921 mRNA Translation: BAE29959.1
AK152096 mRNA Translation: BAE30944.1
AL591426 Genomic DNA No translation available.
BC025209 mRNA Translation: AAH25209.1
BC096385 mRNA Translation: AAH96385.1
BC132307 mRNA Translation: AAI32308.1
CCDSiCCDS25129.1 [Q9CWS1-1]
RefSeqiNP_082295.1, NM_028019.3 [Q9CWS1-1]
UniGeneiMm.22985

3D structure databases

ProteinModelPortaliQ9CWS1
SMRiQ9CWS1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CWS1, 1 interactor
MINTiQ9CWS1
STRINGi10090.ENSMUSP00000017839

PTM databases

iPTMnetiQ9CWS1
PhosphoSitePlusiQ9CWS1

Proteomic databases

EPDiQ9CWS1
PaxDbiQ9CWS1
PeptideAtlasiQ9CWS1
PRIDEiQ9CWS1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000017839; ENSMUSP00000017839; ENSMUSG00000020707 [Q9CWS1-1]
GeneIDi71956
KEGGimmu:71956
UCSCiuc007kln.1 mouse [Q9CWS1-1]

Organism-specific databases

CTDi84282
MGIiMGI:1919206 Rnf135

Phylogenomic databases

eggNOGiKOG2177 Eukaryota
ENOG4111G04 LUCA
GeneTreeiENSGT00830000128449
HOGENOMiHOG000060145
HOVERGENiHBG056606
InParanoidiQ9CWS1
KOiK16272
OMAiNCSHWAV
OrthoDBiEOG091G062V
PhylomeDBiQ9CWS1
TreeFamiTF351089

Enzyme and pathway databases

UniPathwayi
UPA00143

Miscellaneous databases

PROiPR:Q9CWS1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020707 Expressed in 262 organ(s), highest expression level in liver
CleanExiMM_RNF135
ExpressionAtlasiQ9CWS1 baseline and differential
GenevisibleiQ9CWS1 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR001870 B30.2/SPRY
IPR003879 Butyrophylin_SPRY
IPR013320 ConA-like_dom_sf
IPR003877 SPRY_dom
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR017907 Znf_RING_CS
PfamiView protein in Pfam
PF00622 SPRY, 1 hit
PRINTSiPR01407 BUTYPHLNCDUF
SMARTiView protein in SMART
SM00184 RING, 1 hit
SM00449 SPRY, 1 hit
SUPFAMiSSF49899 SSF49899, 1 hit
PROSITEiView protein in PROSITE
PS50188 B302_SPRY, 1 hit
PS00518 ZF_RING_1, 1 hit
PS50089 ZF_RING_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRN135_MOUSE
AccessioniPrimary (citable) accession number: Q9CWS1
Secondary accession number(s): Q3UBK5, Q8R161, Q9DCH3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 20, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 136 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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