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Protein

N(G),N(G)-dimethylarginine dimethylaminohydrolase 1

Gene

Ddah1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.1 Publication

Catalytic activityi

N(omega),N(omega)-dimethyl-L-arginine + H2O = dimethylamine + L-citrulline.

Enzyme regulationi

Inhibited by zinc ions.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei30Substrate; via carbonyl oxygenBy similarity1
Binding sitei73SubstrateBy similarity1
Binding sitei78SubstrateBy similarity1
Binding sitei79SubstrateBy similarity1
Binding sitei98SubstrateBy similarity1
Binding sitei145SubstrateBy similarity1
Active sitei173Proton donorBy similarity1
Binding sitei268Substrate; via carbonyl oxygenBy similarity1
Active sitei274NucleophileBy similarity1
Metal bindingi274ZincBy similarity1

GO - Molecular functioni

  • amino acid binding Source: MGI
  • dimethylargininase activity Source: BHF-UCL
  • zinc ion binding Source: MGI

GO - Biological processi

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.3.18 3474
ReactomeiR-MMU-203615 eNOS activation

Names & Taxonomyi

Protein namesi
Recommended name:
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 (EC:3.5.3.18)
Short name:
DDAH-1
Short name:
Dimethylarginine dimethylaminohydrolase 1
Alternative name(s):
DDAHI
Dimethylargininase-1
Gene namesi
Name:Ddah1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1916469 Ddah1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Pathology & Biotechi

Disruption phenotypei

Death at embryonic stages. Heterozygous mice show no visible phenotype, but have higher than normal tissue and plasma levels of asymmetric dimethylarginine (ADMA). They have increased mean arterial blood pressure and systemic vascular resistance, and decreased cardiac output and heart rate, probably due to reduced levels of nitric oxide (NO).1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001711192 – 285N(G),N(G)-dimethylarginine dimethylaminohydrolase 1Add BLAST284

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei33PhosphoserineBy similarity1
Modified residuei222S-nitrosocysteineBy similarity1
Modified residuei274S-nitrosocysteineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein, S-nitrosylation

Proteomic databases

MaxQBiQ9CWS0
PaxDbiQ9CWS0
PeptideAtlasiQ9CWS0
PRIDEiQ9CWS0

2D gel databases

REPRODUCTION-2DPAGEiQ9CWS0

PTM databases

iPTMnetiQ9CWS0
PhosphoSitePlusiQ9CWS0

Expressioni

Tissue specificityi

Detected in skeletal muscle, lung, heart and brain (at protein level). Detected in liver, kidney and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000028194
CleanExiMM_DDAH1
ExpressionAtlasiQ9CWS0 baseline and differential
GenevisibleiQ9CWS0 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi213300, 1 interactor
IntActiQ9CWS0, 4 interactors
MINTiQ9CWS0
STRINGi10090.ENSMUSP00000029845

Structurei

3D structure databases

ProteinModelPortaliQ9CWS0
SMRiQ9CWS0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the DDAH family.Curated

Phylogenomic databases

eggNOGiENOG410IFS6 Eukaryota
COG1834 LUCA
GeneTreeiENSGT00390000009331
HOGENOMiHOG000161035
HOVERGENiHBG055937
InParanoidiQ9CWS0
KOiK01482
OMAiCNGIALI
OrthoDBiEOG091G0M5S
PhylomeDBiQ9CWS0
TreeFamiTF314737

Family and domain databases

InterProiView protein in InterPro
IPR033199 DDAH/AD
IPR033197 DDAH1
PANTHERiPTHR12737 PTHR12737, 1 hit
PTHR12737:SF17 PTHR12737:SF17, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CWS0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAGLGHPSAF GRATHAVVRA PPESLCRHAL RRSQGEEVDF ARAERQHELY
60 70 80 90 100
VGVLGSKLGL QVVQLPADES LPDCVFVEDV AVVCEETALI TRPGAPSRRK
110 120 130 140 150
EVDMMKEALE KLQLNIVEMK DENATLDGGD VLFTGREFFV GLSKRTNQRG
160 170 180 190 200
AEILADTFKD YAVSTVPVAD SLHLKSFCSM AGPNLIAIGS SESAQKALKI
210 220 230 240 250
MQQMSDHRYD KLTVPDDMAA NCIYLNIPSK GHVLLHRTPE EYPESAKVYE
260 270 280
KLKDHLLIPV SNSEMEKVDG LLTCCSVFIN KKIDS
Length:285
Mass (Da):31,381
Last modified:January 23, 2007 - v3
Checksum:i290B8DDF5AAE7928
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010430 mRNA Translation: BAB26932.1
AK143507 mRNA Translation: BAE25405.1
AK148907 mRNA Translation: BAE28696.1
BC034505 mRNA Translation: AAH34505.1
CCDSiCCDS17896.1
RefSeqiNP_081269.1, NM_026993.3
UniGeneiMm.234247

Genome annotation databases

EnsembliENSMUST00000029845; ENSMUSP00000029845; ENSMUSG00000028194
GeneIDi69219
KEGGimmu:69219
UCSCiuc008rqr.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDDAH1_MOUSE
AccessioniPrimary (citable) accession number: Q9CWS0
Secondary accession number(s): Q3UF65
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 15, 2004
Last sequence update: January 23, 2007
Last modified: June 20, 2018
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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