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Protein

Sorting nexin-2

Gene

Snx2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in several stages of intracellular trafficking. Interacts with membranes containing phosphatidylinositol 3-phosphate (PtdIns(3P)) or phosphatidylinositol 3,5-bisphosphate (PtdIns(3,5)P2). Acts in part as component of the retromer membrane-deforming SNX-BAR subcomplex. The SNX-BAR retromer mediates retrograde transport of cargo proteins from endosomes to the trans-Golgi network (TGN) and is involved in endosome-to-plasma membrane transport for cargo protein recycling. The SNX-BAR subcomplex functions to deform the donor membrane into a tubular profile called endosome-to-TGN transport carrier (ETC). Can sense membrane curvature and has in vitro vesicle-to-membrane remodeling activity. Required for retrograde endosome-to-TGN transport of TGN38. Promotes KALRN- and RHOG-dependent but retromer-independent membrane remodeling such as lamellipodium formation; the function is dependent on GEF activity of KALRN (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei183Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei185Phosphatidylinositol 3-phosphate; via amide nitrogen and carbonyl oxygenBy similarity1
Binding sitei211Phosphatidylinositol 3-phosphateBy similarity1
Binding sitei235Phosphatidylinositol 3-phosphateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processProtein transport, Transport
LigandLipid-binding

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Sorting nexin-2
Gene namesi
Name:Snx2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1915054 Snx2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell projection, Endosome, Membrane

Pathology & Biotechi

Disruption phenotypei

No visible phenotype. Mice are born at the expected Mendelian ratio and are fertile. Mice lacking both Snx1 and Snx2 die during embryonic development, around 9.5 and 11.5 dpc.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002138391 – 519Sorting nexin-2Add BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei97PhosphoserineCombined sources1
Modified residuei101PhosphothreonineCombined sources1
Modified residuei104PhosphothreonineCombined sources1
Modified residuei117PhosphoserineBy similarity1
Modified residuei119PhosphoserineCombined sources1
Modified residuei185PhosphoserineCombined sources1
Modified residuei277PhosphoserineBy similarity1
Modified residuei469N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CWK8
MaxQBiQ9CWK8
PaxDbiQ9CWK8
PeptideAtlasiQ9CWK8
PRIDEiQ9CWK8

PTM databases

iPTMnetiQ9CWK8
PhosphoSitePlusiQ9CWK8

Expressioni

Gene expression databases

BgeeiENSMUSG00000034484 Expressed in 102 organ(s), highest expression level in embryo
CleanExiMM_SNX2
GenevisibleiQ9CWK8 MM

Interactioni

Subunit structurei

Predominantly forms heterodimers with BAR domain-containing sorting nexins SNX5, SNX6 and SNX32; can self-associate to form homodimers. The heterodimers are proposed to self-assemble into helical arrays on the membrane to stabilize and expand local membrane curvature underlying endosomal tubule formation. Thought to be a component of the originally described retromer complex (also called SNX-BAR retromer) which is a pentamer containing the heterotrimeric retromer cargo-selective complex (CSC), also descibed as vacuolar protein sorting subcomplex (VPS), and a heterodimeric membrane-deforming subcomplex formed between SNX1 or SNX2 and SNX5 or SNX6 (also called SNX-BAR subcomplex); the respective CSC and SNX-BAR subcomplexes associate with low affinity. Interacts with SNX5, SNX6, SNX32, VPS26A, VPS29, VPS35, FNBP1, KALRN, RHOG (GDP-bound form) (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212452, 2 interactors
STRINGi10090.ENSMUSP00000039243

Structurei

3D structure databases

ProteinModelPortaliQ9CWK8
SMRiQ9CWK8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini140 – 269PXPROSITE-ProRule annotationAdd BLAST130
Domaini299 – 519BARBy similarityAdd BLAST221

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni260 – 519Interaction with RhoGBy similarityAdd BLAST260
Regioni278 – 295Membrane-binding amphipathic helixBy similarityAdd BLAST18

Domaini

The BAR domain is able to sense membrane curvature upon dimerization. Membrane remodeling seems to implicate insertion of a N-terminal amphipatric helix (AH) in the membrane (By similarity).By similarity

Sequence similaritiesi

Belongs to the sorting nexin family.Curated

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
GeneTreeiENSGT00930000150904
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiQ9CWK8
KOiK17917
OMAiPRMESKS
OrthoDBiEOG091G06FB
PhylomeDBiQ9CWK8
TreeFamiTF313698

Family and domain databases

CDDicd07282 PX_SNX2, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR028653 SNX2
IPR037918 SNX2_PX
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF31 PTHR10555:SF31, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CWK8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAEREPPPL GDVKPTDFEE LEDGEDLFTS TVSTLESSPS SPEPASLPAE
60 70 80 90 100
DISANSNGSK PVEVVLDDDR EDLFAEATEE VSLDSPEREL ILSSEPSPAV
110 120 130 140 150
TPVTPTTLIA PRIESKSISA PVIFDRSRDE IEEEANGDIF DIEIGVSDPE
160 170 180 190 200
KVGDGMNAYM AYRVTTKTSL SMFSKSEFSV KRRFSDFLGL HSKLASKYLH
210 220 230 240 250
VGYIVPPAPE KSIVGMTKVK VGKEDSSSTE FVEKRRAALE RYLQRTVKHP
260 270 280 290 300
TLLQDPDLRQ FLESSELPRA VNTQALSGAG ILRMVNKAAD AVNKMTIKMN
310 320 330 340 350
ESDAWFEEKQ QQFENLDQQL RKLHASVEAL VCHRKELSAN TAAFAKSAAM
360 370 380 390 400
LGNSEDHTAL SRALSQLAEV EEKIDQLHQE QAFADFYMFS ELLSDYIRLI
410 420 430 440 450
AAVKGVFDHR MKCWQKWEDA QITLLKKRET EAKMMVANKP DKIQQAKNEI
460 470 480 490 500
REWEAKVQQG ERDFEQISKT IRKEVGRFEK ERVKDFKAVI IKYLESLVQT
510
QQQLIKYWEA FLPEAKAIA
Length:519
Mass (Da):58,471
Last modified:March 27, 2002 - v2
Checksum:i55DD0BB74E82CD82
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti245R → I in BAB27035 (PubMed:16141072).Curated1
Sequence conflicti428R → P in BAB27035 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002692 mRNA Translation: BAB22287.1
AK005470 mRNA Translation: BAB24060.1
AK010572 mRNA Translation: BAB27035.1
AK075929 mRNA Translation: BAC36060.1
AK145840 mRNA Translation: BAE26688.1
CCDSiCCDS37821.1
RefSeqiNP_080662.1, NM_026386.1
UniGeneiMm.252171

Genome annotation databases

EnsembliENSMUST00000037850; ENSMUSP00000039243; ENSMUSG00000034484
GeneIDi67804
KEGGimmu:67804
UCSCiuc008exr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002692 mRNA Translation: BAB22287.1
AK005470 mRNA Translation: BAB24060.1
AK010572 mRNA Translation: BAB27035.1
AK075929 mRNA Translation: BAC36060.1
AK145840 mRNA Translation: BAE26688.1
CCDSiCCDS37821.1
RefSeqiNP_080662.1, NM_026386.1
UniGeneiMm.252171

3D structure databases

ProteinModelPortaliQ9CWK8
SMRiQ9CWK8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212452, 2 interactors
STRINGi10090.ENSMUSP00000039243

PTM databases

iPTMnetiQ9CWK8
PhosphoSitePlusiQ9CWK8

Proteomic databases

EPDiQ9CWK8
MaxQBiQ9CWK8
PaxDbiQ9CWK8
PeptideAtlasiQ9CWK8
PRIDEiQ9CWK8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000037850; ENSMUSP00000039243; ENSMUSG00000034484
GeneIDi67804
KEGGimmu:67804
UCSCiuc008exr.1 mouse

Organism-specific databases

CTDi6643
MGIiMGI:1915054 Snx2

Phylogenomic databases

eggNOGiKOG2273 Eukaryota
COG5391 LUCA
GeneTreeiENSGT00930000150904
HOGENOMiHOG000293327
HOVERGENiHBG000618
InParanoidiQ9CWK8
KOiK17917
OMAiPRMESKS
OrthoDBiEOG091G06FB
PhylomeDBiQ9CWK8
TreeFamiTF313698

Enzyme and pathway databases

ReactomeiR-MMU-432722 Golgi Associated Vesicle Biogenesis

Miscellaneous databases

ChiTaRSiSnx2 mouse
PROiPR:Q9CWK8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000034484 Expressed in 102 organ(s), highest expression level in embryo
CleanExiMM_SNX2
GenevisibleiQ9CWK8 MM

Family and domain databases

CDDicd07282 PX_SNX2, 1 hit
Gene3Di1.20.1270.60, 1 hit
3.30.1520.10, 1 hit
InterProiView protein in InterPro
IPR027267 AH/BAR_dom_sf
IPR001683 Phox
IPR036871 PX_dom_sf
IPR028653 SNX2
IPR037918 SNX2_PX
IPR039358 Sorting_nexin-like
IPR005329 Sorting_nexin_N
IPR015404 Vps5_C
PANTHERiPTHR10555 PTHR10555, 1 hit
PTHR10555:SF31 PTHR10555:SF31, 1 hit
PfamiView protein in Pfam
PF00787 PX, 1 hit
PF03700 Sorting_nexin, 1 hit
PF09325 Vps5, 1 hit
SMARTiView protein in SMART
SM00312 PX, 1 hit
SUPFAMiSSF103657 SSF103657, 1 hit
SSF64268 SSF64268, 1 hit
PROSITEiView protein in PROSITE
PS50195 PX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSNX2_MOUSE
AccessioniPrimary (citable) accession number: Q9CWK8
Secondary accession number(s): Q3UKW3, Q9CQV0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: March 27, 2002
Last modified: November 7, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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