UniProtKB - Q9CWH6 (PSMA8_MOUSE)
Proteasome subunit alpha type-8
Psma8
Functioni
Caution
GO - Molecular functioni
- endopeptidase activity Source: GO_Central
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- meiotic cell cycle Source: UniProtKB
- proteasomal protein catabolic process Source: GO_Central
- proteasomal ubiquitin-independent protein catabolic process Source: GO_Central
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: GO_Central
- regulation of meiosis I Source: UniProtKB
- spermatogenesis Source: UniProtKB-KW
Keywordsi
Biological process | Differentiation, Spermatogenesis |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Protein family/group databases
MEROPSi | T01.978 |
Names & Taxonomyi
Protein namesi | Recommended name: Proteasome subunit alpha type-81 PublicationAlternative name(s): Proteasome alpha 4 subunit1 Publication Short name: Alpha4s1 Publication Proteasome subunit alpha-type 7-like |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1920927, Psma8 |
Subcellular locationi
Nucleus
- Nucleus 1 Publication
Note: Localizes to the central region of the synaptonemal complex.1 Publication
Cytosol
- cytosol Source: Reactome
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: GO_Central
- proteasome core complex Source: GO_Central
- proteasome core complex, alpha-subunit complex Source: UniProtKB
- spermatoproteasome complex Source: UniProtKB
Keywords - Cellular componenti
Nucleus, ProteasomePathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124150 | 1 – 250 | Proteasome subunit alpha type-8Add BLAST | 250 |
Proteomic databases
jPOSTi | Q9CWH6 |
MaxQBi | Q9CWH6 |
PaxDbi | Q9CWH6 |
PeptideAtlasi | Q9CWH6 |
PRIDEi | Q9CWH6 |
PTM databases
iPTMneti | Q9CWH6 |
PhosphoSitePlusi | Q9CWH6 |
Expressioni
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000036743, Expressed in spermatocyte and 101 other tissues |
ExpressionAtlasi | Q9CWH6, baseline and differential |
Genevisiblei | Q9CWH6, MM |
Interactioni
Subunit structurei
Component of the outer alpha-ring of the 20S proteasome core which is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure (PubMed:23706739, PubMed:31358751). The catalytic chamber with the active sites is on the inside of the barrel (Probable).
Interacts with canonical subunits of the spermatoproteasome, including proteasome activators PSME4 (also called PA200) and PSME3 (also called PA28-gamma) (PubMed:31437213).
Interacts with proteasome-interacting proteins chaperones including CCT6B and CCT2, ubiquitin ligases (TRIP12, NEDD4, TRIM36 and RAD18), and ubiquitin specific proteases such as USP9X, USP34, USP5 and USP47 (PubMed:31437213).
Interacts with meiotic proteins cyclin dependent kinase CDK1 and the ATPase TRIP13 as well as proteins of the synaptonemal complex SIX6OS1 and SYCE3 (PubMed:31437213).
Curated3 PublicationsProtein-protein interaction databases
BioGRIDi | 216184, 1 interactor |
IntActi | Q9CWH6, 2 interactors |
MINTi | Q9CWH6 |
STRINGi | 10090.ENSMUSP00000042590 |
Miscellaneous databases
RNActi | Q9CWH6, protein |
Family & Domainsi
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0183, Eukaryota |
GeneTreei | ENSGT00940000160354 |
HOGENOMi | CLU_035750_4_0_1 |
InParanoidi | Q9CWH6 |
OMAi | HEVTTKC |
OrthoDBi | 1222564at2759 |
PhylomeDBi | Q9CWH6 |
TreeFami | TF106212 |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR000426, Proteasome_asu_N IPR001353, Proteasome_sua/b |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASRYDRAIT VFSPDGHLFQ VEYAQEAVKK GSTAVGIRGT NIVVLGVEKK
60 70 80 90 100
SVAKLQDERT VRKICALDDH VCMAFAGLTA DARVVISRAR VECQSHKLTV
110 120 130 140 150
EDPVTVEYIT RFIATLKQKY TQSNGRRPFG ISALIVGFDD DGIPRLYQTD
160 170 180 190 200
PSGTYHAWKA NAIGRSAKTV REFLEKNYTE DAISNDKEAI KLAIKALLEV
210 220 230 240 250
VQSGGKNIEL AIIRRDQPLK MFSAKEIELE VSEIEREKDE AEKTKSKKST
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketB7ZMS4 | B7ZMS4_MOUSE | Proteasome subunit alpha type | Psma8 | 210 | Annotation score: | ||
A0A3Q4EG42 | A0A3Q4EG42_MOUSE | Proteasome subunit alpha type-8 | Psma8 | 63 | Annotation score: | ||
A0A3Q4EHS3 | A0A3Q4EHS3_MOUSE | Proteasome subunit alpha type-8 | Psma8 | 53 | Annotation score: | ||
A0A3Q4L361 | A0A3Q4L361_MOUSE | Proteasome subunit alpha type-8 | Psma8 | 35 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK010717 mRNA Translation: BAB27139.1 BC116990 mRNA Translation: AAI16991.1 BC116992 mRNA Translation: AAI16993.1 |
CCDSi | CCDS50226.1 |
RefSeqi | NP_001157081.1, NM_001163609.1 |
Genome annotation databases
Ensembli | ENSMUST00000040860; ENSMUSP00000042590; ENSMUSG00000036743 |
GeneIDi | 73677 |
KEGGi | mmu:73677 |
UCSCi | uc008edj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK010717 mRNA Translation: BAB27139.1 BC116990 mRNA Translation: AAI16991.1 BC116992 mRNA Translation: AAI16993.1 |
CCDSi | CCDS50226.1 |
RefSeqi | NP_001157081.1, NM_001163609.1 |
3D structure databases
SMRi | Q9CWH6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 216184, 1 interactor |
IntActi | Q9CWH6, 2 interactors |
MINTi | Q9CWH6 |
STRINGi | 10090.ENSMUSP00000042590 |
Protein family/group databases
MEROPSi | T01.978 |
PTM databases
iPTMneti | Q9CWH6 |
PhosphoSitePlusi | Q9CWH6 |
Proteomic databases
jPOSTi | Q9CWH6 |
MaxQBi | Q9CWH6 |
PaxDbi | Q9CWH6 |
PeptideAtlasi | Q9CWH6 |
PRIDEi | Q9CWH6 |
Protocols and materials databases
Antibodypediai | 22107, 67 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000040860; ENSMUSP00000042590; ENSMUSG00000036743 |
GeneIDi | 73677 |
KEGGi | mmu:73677 |
UCSCi | uc008edj.2, mouse |
Organism-specific databases
CTDi | 143471 |
MGIi | MGI:1920927, Psma8 |
Phylogenomic databases
eggNOGi | KOG0183, Eukaryota |
GeneTreei | ENSGT00940000160354 |
HOGENOMi | CLU_035750_4_0_1 |
InParanoidi | Q9CWH6 |
OMAi | HEVTTKC |
OrthoDBi | 1222564at2759 |
PhylomeDBi | Q9CWH6 |
TreeFami | TF106212 |
Enzyme and pathway databases
Reactomei | R-MMU-1169091, Activation of NF-kappaB in B cells R-MMU-1234176, Oxygen-dependent proline hydroxylation of Hypoxia-inducible Factor Alpha R-MMU-1236978, Cross-presentation of soluble exogenous antigens (endosomes) R-MMU-174154, APC/C:Cdc20 mediated degradation of Securin R-MMU-174178, APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1 R-MMU-174184, Cdc20:Phospho-APC/C mediated degradation of Cyclin A R-MMU-195253, Degradation of beta-catenin by the destruction complex R-MMU-202424, Downstream TCR signaling R-MMU-2467813, Separation of Sister Chromatids R-MMU-2871837, FCERI mediated NF-kB activation R-MMU-349425, Autodegradation of the E3 ubiquitin ligase COP1 R-MMU-350562, Regulation of ornithine decarboxylase (ODC) R-MMU-382556, ABC-family proteins mediated transport R-MMU-450408, AUF1 (hnRNP D0) binds and destabilizes mRNA R-MMU-4608870, Asymmetric localization of PCP proteins R-MMU-4641257, Degradation of AXIN R-MMU-4641258, Degradation of DVL R-MMU-5358346, Hedgehog ligand biogenesis R-MMU-5607761, Dectin-1 mediated noncanonical NF-kB signaling R-MMU-5607764, CLEC7A (Dectin-1) signaling R-MMU-5610780, Degradation of GLI1 by the proteasome R-MMU-5610785, GLI3 is processed to GLI3R by the proteasome R-MMU-5632684, Hedgehog 'on' state R-MMU-5658442, Regulation of RAS by GAPs R-MMU-5668541, TNFR2 non-canonical NF-kB pathway R-MMU-5676590, NIK-->noncanonical NF-kB signaling R-MMU-5687128, MAPK6/MAPK4 signaling R-MMU-5689603, UCH proteinases R-MMU-5689880, Ub-specific processing proteases R-MMU-68827, CDT1 association with the CDC6:ORC:origin complex R-MMU-68949, Orc1 removal from chromatin R-MMU-69017, CDK-mediated phosphorylation and removal of Cdc6 R-MMU-69481, G2/M Checkpoints R-MMU-69601, Ubiquitin Mediated Degradation of Phosphorylated Cdc25A R-MMU-75815, Ubiquitin-dependent degradation of Cyclin D R-MMU-8852276, The role of GTSE1 in G2/M progression after G2 checkpoint R-MMU-8854050, FBXL7 down-regulates AURKA during mitotic entry and in early mitosis R-MMU-8939236, RUNX1 regulates transcription of genes involved in differentiation of HSCs R-MMU-8948751, Regulation of PTEN stability and activity R-MMU-8951664, Neddylation R-MMU-9020702, Interleukin-1 signaling R-MMU-983168, Antigen processing: Ubiquitination & Proteasome degradation |
Miscellaneous databases
BioGRID-ORCSi | 73677, 0 hits in 17 CRISPR screens |
ChiTaRSi | Psma8, mouse |
PROi | PR:Q9CWH6 |
RNActi | Q9CWH6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000036743, Expressed in spermatocyte and 101 other tissues |
ExpressionAtlasi | Q9CWH6, baseline and differential |
Genevisiblei | Q9CWH6, MM |
Family and domain databases
Gene3Di | 3.60.20.10, 1 hit |
InterProi | View protein in InterPro IPR029055, Ntn_hydrolases_N IPR023332, Proteasome_alpha-type IPR000426, Proteasome_asu_N IPR001353, Proteasome_sua/b |
Pfami | View protein in Pfam PF00227, Proteasome, 1 hit PF10584, Proteasome_A_N, 1 hit |
SMARTi | View protein in SMART SM00948, Proteasome_A_N, 1 hit |
SUPFAMi | SSF56235, SSF56235, 1 hit |
PROSITEi | View protein in PROSITE PS00388, PROTEASOME_ALPHA_1, 1 hit PS51475, PROTEASOME_ALPHA_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PSMA8_MOUSE | |
Accessioni | Q9CWH6Primary (citable) accession number: Q9CWH6 Secondary accession number(s): Q14A44 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families - Peptidase families
Classification of peptidase families and list of entries