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Protein

E3 ubiquitin-protein ligase SMURF1

Gene

Smurf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

E3 ubiquitin-protein ligase that acts as a negative regulator of BMP signaling pathway (By similarity). Mediates ubiquitination and degradation of SMAD1 and SMAD5, 2 receptor-regulated SMADs specific for the BMP pathway (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of TRAF family members and RHOA (By similarity). Promotes ubiquitination and subsequent proteasomal degradation of MAVS (PubMed:23087404). Plays a role in dendrite formation by melanocytes (By similarity).By similarity1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.By similarity

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei699Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processDifferentiation, Ubl conjugation pathway

Enzyme and pathway databases

BRENDAi6.3.2.19 3474
ReactomeiR-MMU-201451 Signaling by BMP
R-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-5632684 Hedgehog 'on' state
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SMURF1 (EC:2.3.2.26By similarity)
Alternative name(s):
HECT-type E3 ubiquitin transferase SMURF1
SMAD ubiquitination regulatory factor 1
SMAD-specific E3 ubiquitin-protein ligase 1
Gene namesi
Name:Smurf1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1923038 Smurf1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001203271 – 731E3 ubiquitin-protein ligase SMURF1Add BLAST731

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei200PhosphoserineBy similarity1
Cross-linki355Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Cross-linki357Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity

Post-translational modificationi

Auto-ubiquitinated in presence of NDFIP1. Ubiquitinated by the SCF(FBXL15) complex at Lys-355 and Lys-357, leading to its degradation by the proteasome. Lys-357 is the primary ubiquitination site.By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9CUN6
PaxDbiQ9CUN6
PRIDEiQ9CUN6

PTM databases

iPTMnetiQ9CUN6
PhosphoSitePlusiQ9CUN6

Expressioni

Gene expression databases

BgeeiENSMUSG00000038780 Expressed in 236 organ(s), highest expression level in submandibular gland
CleanExiMM_SMURF1
ExpressionAtlasiQ9CUN6 baseline and differential
GenevisibleiQ9CUN6 MM

Interactioni

Subunit structurei

Interacts with TRAF4 (By similarity). Interacts (via HECT domain) with FBXL15 (via LRR repeats) (By similarity). Interacts with SMAD7 and TGFBR1; SMAD7 recruits SMURF1 to TGFBR1 and regulates TGF-beta receptor degradation (By similarity). Interacts with MAVS; the interaction is mediated by NDFIP1 (PubMed:23087404).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi217743, 14 interactors
CORUMiQ9CUN6
IntActiQ9CUN6, 3 interactors
MINTiQ9CUN6
STRINGi10090.ENSMUSP00000082827

Structurei

3D structure databases

ProteinModelPortaliQ9CUN6
SMRiQ9CUN6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1 – 99C2PROSITE-ProRule annotationAdd BLAST99
Domaini234 – 267WW 1PROSITE-ProRule annotationAdd BLAST34
Domaini280 – 313WW 2PROSITE-ProRule annotationAdd BLAST34
Domaini394 – 731HECTPROSITE-ProRule annotationAdd BLAST338

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi144 – 147Poly-Gly4

Domaini

The C2 domain mediates membrane localization and substrate selection.By similarity

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0940 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00760000118966
HOGENOMiHOG000208451
HOVERGENiHBG004134
InParanoidiQ9CUN6
KOiK04678
PhylomeDBiQ9CUN6
TreeFamiTF323658

Family and domain databases

CDDicd00078 HECTc, 1 hit
cd00201 WW, 2 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR024928 E3_ub_ligase_SMURF1
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00632 HECT, 1 hit
PF00397 WW, 2 hits
PIRSFiPIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00119 HECTc, 1 hit
SM00456 WW, 2 hits
SUPFAMiSSF51045 SSF51045, 2 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50237 HECT, 1 hit
PS01159 WW_DOMAIN_1, 1 hit
PS50020 WW_DOMAIN_2, 2 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9CUN6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSNPGTRRNG SSIKIRLTVL CAKNLAKKDF FRLPDPFAKI VVDGSGQCHS
60 70 80 90 100
TDTVKNTLDP KWNQHYDLYV GKTDSITISV WNHKKIHKKQ GAGFLGCVRL
110 120 130 140 150
LSNAISRLKD TGYQRLDLCK LNPSDTDAVR GQIVVSLQTR DRIGGGGSVV
160 170 180 190 200
DCRGLLENEG TVYEDSGPGR PLSCLMEEPA PYTDGTGAAA GGGNCRFVES
210 220 230 240 250
PSQDQRLLVQ RLRNPEVRGP LQTPQNRPHG HQSPELPEGY EQRTTVQGQV
260 270 280 290 300
YFLHTQTGVS TWHDPRIPRD LNSVNCDELG PLPPGWEVRS TVSGRIYFVD
310 320 330 340 350
HNNRTTQFTD PRLHHIMNHQ CQLKEPSQPL QLPSEGSVED EELPAQRYER
360 370 380 390 400
DLVQKLKVLR HELSLQQPQA GHCRIEVSRE EIFEESYRQI MKMRPKDLKK
410 420 430 440 450
RLMVKFRGEE GLDYGGVARE WLYLLCHEML NPYYGLFQYS TDNIYTLQIN
460 470 480 490 500
PDSSINPDHL SYFHFVGRIM GLAVFHGHYI NGGFTVPFYK QLLGKPIQLS
510 520 530 540 550
DLESVDPELH KSLVWILEND ITPVLDHTFC VEHNAFGRIL QHELKPNGRN
560 570 580 590 600
VPVTEENKKE YVRLYVNWRF MRGIEAQFLA LQKGFNELIP QHLLKPFDQK
610 620 630 640 650
ELELIIGGLD KIDLNDWKSN TRLKHCVADS NIVRWFWQAV ETFDEERRAR
660 670 680 690 700
LLQFVTGSTR VPLQGFKALQ GSTGAAGPRL FTIHLIDANT DNLPKAHTCF
710 720 730
NRIDIPPYES YEKLYEKLLT AVEETCGFAV E
Length:731
Mass (Da):83,356
Last modified:January 10, 2006 - v2
Checksum:iF0A7A1B019CC9D3E
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
E9PYU8E9PYU8_MOUSE
E3 ubiquitin-protein ligase
Smurf1
757Annotation score:
E9Q4K9E9Q4K9_MOUSE
E3 ubiquitin-protein ligase
Smurf1
728Annotation score:
A0A0G2JEN5A0A0G2JEN5_MOUSE
E3 ubiquitin-protein ligase SMURF1
Smurf1
309Annotation score:

Sequence cautioni

The sequence BAB29770 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti63N → S in AAH29097 (PubMed:15489334).Curated1
Sequence conflicti204D → N in BAB29770 (PubMed:16141072).Curated1
Sequence conflicti213R → Q in BAB29770 (PubMed:16141072).Curated1
Sequence conflicti218R → Q in BAB29770 (PubMed:16141072).Curated1
Sequence conflicti278E → G in BAE32623 (PubMed:16141072).Curated1
Sequence conflicti334S → N in BAB29770 (PubMed:16141072).Curated1
Sequence conflicti446T → K in BAB29770 (PubMed:16141072).Curated1
Sequence conflicti671 – 673Missing in BAB29770 (PubMed:16141072).Curated3
Sequence conflicti671 – 673Missing in AAH29097 (PubMed:15489334).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015264 mRNA Translation: BAB29770.2 Different initiation.
AK154491 mRNA Translation: BAE32623.1
BC029097 mRNA Translation: AAH29097.1
CCDSiCCDS39380.1
RefSeqiNP_001033716.1, NM_001038627.1
NP_083714.3, NM_029438.3
UniGeneiMm.27735

Genome annotation databases

EnsembliENSMUST00000085684; ENSMUSP00000082827; ENSMUSG00000038780
GeneIDi75788
KEGGimmu:75788
UCSCiuc009alv.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK015264 mRNA Translation: BAB29770.2 Different initiation.
AK154491 mRNA Translation: BAE32623.1
BC029097 mRNA Translation: AAH29097.1
CCDSiCCDS39380.1
RefSeqiNP_001033716.1, NM_001038627.1
NP_083714.3, NM_029438.3
UniGeneiMm.27735

3D structure databases

ProteinModelPortaliQ9CUN6
SMRiQ9CUN6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217743, 14 interactors
CORUMiQ9CUN6
IntActiQ9CUN6, 3 interactors
MINTiQ9CUN6
STRINGi10090.ENSMUSP00000082827

PTM databases

iPTMnetiQ9CUN6
PhosphoSitePlusiQ9CUN6

Proteomic databases

MaxQBiQ9CUN6
PaxDbiQ9CUN6
PRIDEiQ9CUN6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000085684; ENSMUSP00000082827; ENSMUSG00000038780
GeneIDi75788
KEGGimmu:75788
UCSCiuc009alv.1 mouse

Organism-specific databases

CTDi57154
MGIiMGI:1923038 Smurf1

Phylogenomic databases

eggNOGiKOG0940 Eukaryota
COG5021 LUCA
GeneTreeiENSGT00760000118966
HOGENOMiHOG000208451
HOVERGENiHBG004134
InParanoidiQ9CUN6
KOiK04678
PhylomeDBiQ9CUN6
TreeFamiTF323658

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi6.3.2.19 3474
ReactomeiR-MMU-201451 Signaling by BMP
R-MMU-2173791 TGF-beta receptor signaling in EMT (epithelial to mesenchymal transition)
R-MMU-4608870 Asymmetric localization of PCP proteins
R-MMU-5632684 Hedgehog 'on' state
R-MMU-8941858 Regulation of RUNX3 expression and activity
R-MMU-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q9CUN6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000038780 Expressed in 236 organ(s), highest expression level in submandibular gland
CleanExiMM_SMURF1
ExpressionAtlasiQ9CUN6 baseline and differential
GenevisibleiQ9CUN6 MM

Family and domain databases

CDDicd00078 HECTc, 1 hit
cd00201 WW, 2 hits
Gene3Di2.60.40.150, 1 hit
InterProiView protein in InterPro
IPR000008 C2_dom
IPR035892 C2_domain_sf
IPR024928 E3_ub_ligase_SMURF1
IPR000569 HECT_dom
IPR035983 Hect_E3_ubiquitin_ligase
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00168 C2, 1 hit
PF00632 HECT, 1 hit
PF00397 WW, 2 hits
PIRSFiPIRSF001569 E3_ub_ligase_SMURF1, 1 hit
SMARTiView protein in SMART
SM00239 C2, 1 hit
SM00119 HECTc, 1 hit
SM00456 WW, 2 hits
SUPFAMiSSF51045 SSF51045, 2 hits
SSF56204 SSF56204, 1 hit
PROSITEiView protein in PROSITE
PS50004 C2, 1 hit
PS50237 HECT, 1 hit
PS01159 WW_DOMAIN_1, 1 hit
PS50020 WW_DOMAIN_2, 2 hits
ProtoNetiSearch...

Entry informationi

Entry nameiSMUF1_MOUSE
AccessioniPrimary (citable) accession number: Q9CUN6
Secondary accession number(s): Q3U412, Q8K300
Entry historyiIntegrated into UniProtKB/Swiss-Prot: December 13, 2001
Last sequence update: January 10, 2006
Last modified: November 7, 2018
This is version 161 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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