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UniProtKB - Q9CRA4 (MSMO1_MOUSE)
Protein
Methylsterol monooxygenase 1
Gene
Msmo1
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, which can be subsequently metabolized to cholesterol.
By similarityCatalytic activityi
- 4,4-dimethyl-5α-cholest-7-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β-methyl-5α-cholest-7-ene-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityEC:1.14.18.9By similarityThis reaction proceeds in the forwardBy similarity direction.
- 4,4-dimethyl-5α-cholesta-8,24-dien-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4β-methylzymosterol-4α-carboxylate + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4α-methylzymosterol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxyzymosterol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4α-methyl-5α-cholest-7-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-5α-cholest-7-en-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4,4-dimethyl-5α-cholest-8-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-4β-methyl-5α-cholest-8-en-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
- 4α-methyl-5α-cholest-8-en-3β-ol + 6 Fe(II)-[cytochrome b5] + 5 H+ + 3 O2 = 4α-carboxy-5α-cholest-8-ene-3β-ol + 6 Fe(III)-[cytochrome b5] + 4 H2OBy similarityThis reaction proceeds in the forwardBy similarity direction.
Cofactori
Fe cationBy similarity
: zymosterol biosynthesis Pathwayi
This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.By similarity This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.
Pathwayi: cholesterol biosynthesis
This protein is involved in the pathway cholesterol biosynthesis, which is part of Steroid biosynthesis.By similarityView all proteins of this organism that are known to be involved in the pathway cholesterol biosynthesis and in Steroid biosynthesis.
GO - Molecular functioni
- C-4 methylsterol oxidase activity Source: GO_Central
- iron ion binding Source: InterPro
- oxidoreductase activity Source: GO_Central
GO - Biological processi
- cholesterol biosynthetic process Source: UniProtKB-UniPathway
- sterol biosynthetic process Source: GO_Central
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Cholesterol biosynthesis, Cholesterol metabolism, Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism |
Ligand | Iron, NAD |
Enzyme and pathway databases
Reactomei | R-MMU-191273, Cholesterol biosynthesis |
UniPathwayi | UPA00063 UPA00770;UER00756 |
Names & Taxonomyi
Protein namesi | Recommended name: Methylsterol monooxygenase 1 (EC:1.14.18.9By similarity)Alternative name(s): C-4 methylsterol oxidase Sterol-C4-methyl oxidase |
Gene namesi | Name:Msmo1 Synonyms:Sc4mol |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913484, Msmo1 |
VEuPathDBi | HostDB:ENSMUSG00000031604 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: GO_Central
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: GO_Central
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 55 – 75 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 100 – 120 | HelicalSequence analysisAdd BLAST | 21 | |
Transmembranei | 199 – 219 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000117034 | 1 – 293 | Methylsterol monooxygenase 1Add BLAST | 293 |
Proteomic databases
EPDi | Q9CRA4 |
jPOSTi | Q9CRA4 |
MaxQBi | Q9CRA4 |
PaxDbi | Q9CRA4 |
PeptideAtlasi | Q9CRA4 |
PRIDEi | Q9CRA4 |
ProteomicsDBi | 290101 |
PTM databases
iPTMneti | Q9CRA4 |
PhosphoSitePlusi | Q9CRA4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031604, Expressed in sciatic nerve and 338 other tissues |
ExpressionAtlasi | Q9CRA4, baseline and differential |
Genevisiblei | Q9CRA4, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 211315, 3 interactors |
STRINGi | 10090.ENSMUSP00000034015 |
Miscellaneous databases
RNActi | Q9CRA4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 144 – 274 | Fatty acid hydroxylaseSequence analysisAdd BLAST | 131 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 157 – 161 | Histidine box-1 | 5 | |
Motifi | 170 – 174 | Histidine box-2 | 5 | |
Motifi | 249 – 255 | Histidine box-3 | 7 |
Domaini
The histidine box domains may contain the active site and/or be involved in metal ion binding.
Sequence similaritiesi
Belongs to the sterol desaturase family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0873, Eukaryota |
GeneTreei | ENSGT00940000158012 |
HOGENOMi | CLU_047036_5_2_1 |
InParanoidi | Q9CRA4 |
OMAi | PGFIFQF |
OrthoDBi | 1493916at2759 |
PhylomeDBi | Q9CRA4 |
TreeFami | TF354294 |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9CRA4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATNKSVGVF SSASLAVEYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA
60 70 80 90 100
TWGSLIVHEA IYFLFSLPGF LFQFIPYMRK YKIQKDKPET FEGQWKCLKK
110 120 130 140 150
ILFNHFFIQL PLICGTYYFT EFFNIPYDWE RMPRWYLTLA RCLGCAVIED
160 170 180 190 200
TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF GIEAEYAHPL ETLILGTGFF
210 220 230 240 250
IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL VPFYTGARHH
260 270 280 290
DFHHMNFIGN YASTFTWWDK LFGTDAQYHA YIEKSKKLGK KSD
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3YTN3 | D3YTN3_MOUSE | Methylsterol monooxygenase 1 | Msmo1 | 76 | Annotation score: | ||
D3Z458 | D3Z458_MOUSE | Methylsterol monooxygenase 1 | Msmo1 | 109 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK005090 mRNA Translation: BAB23811.1 AK005441 mRNA Translation: BAB24035.1 AK045059 mRNA Translation: BAC32201.1 AK146684 mRNA Translation: BAE27358.1 AK151135 mRNA Translation: BAE30143.1 AK169017 mRNA Translation: BAE40814.1 BC006802 mRNA Translation: AAH06802.1 |
CCDSi | CCDS22328.1 |
RefSeqi | NP_079712.1, NM_025436.2 |
Genome annotation databases
Ensembli | ENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604 |
GeneIDi | 66234 |
KEGGi | mmu:66234 |
UCSCi | uc009lux.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK005090 mRNA Translation: BAB23811.1 AK005441 mRNA Translation: BAB24035.1 AK045059 mRNA Translation: BAC32201.1 AK146684 mRNA Translation: BAE27358.1 AK151135 mRNA Translation: BAE30143.1 AK169017 mRNA Translation: BAE40814.1 BC006802 mRNA Translation: AAH06802.1 |
CCDSi | CCDS22328.1 |
RefSeqi | NP_079712.1, NM_025436.2 |
3D structure databases
SMRi | Q9CRA4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211315, 3 interactors |
STRINGi | 10090.ENSMUSP00000034015 |
PTM databases
iPTMneti | Q9CRA4 |
PhosphoSitePlusi | Q9CRA4 |
Proteomic databases
EPDi | Q9CRA4 |
jPOSTi | Q9CRA4 |
MaxQBi | Q9CRA4 |
PaxDbi | Q9CRA4 |
PeptideAtlasi | Q9CRA4 |
PRIDEi | Q9CRA4 |
ProteomicsDBi | 290101 |
Protocols and materials databases
Antibodypediai | 28315, 131 antibodies from 19 providers |
DNASUi | 66234 |
Genome annotation databases
Ensembli | ENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604 |
GeneIDi | 66234 |
KEGGi | mmu:66234 |
UCSCi | uc009lux.1, mouse |
Organism-specific databases
CTDi | 6307 |
MGIi | MGI:1913484, Msmo1 |
VEuPathDBi | HostDB:ENSMUSG00000031604 |
Phylogenomic databases
eggNOGi | KOG0873, Eukaryota |
GeneTreei | ENSGT00940000158012 |
HOGENOMi | CLU_047036_5_2_1 |
InParanoidi | Q9CRA4 |
OMAi | PGFIFQF |
OrthoDBi | 1493916at2759 |
PhylomeDBi | Q9CRA4 |
TreeFami | TF354294 |
Enzyme and pathway databases
UniPathwayi | UPA00063 UPA00770;UER00756 |
Reactomei | R-MMU-191273, Cholesterol biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 66234, 9 hits in 66 CRISPR screens |
ChiTaRSi | Msmo1, mouse |
PROi | PR:Q9CRA4 |
RNActi | Q9CRA4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031604, Expressed in sciatic nerve and 338 other tissues |
ExpressionAtlasi | Q9CRA4, baseline and differential |
Genevisiblei | Q9CRA4, MM |
Family and domain databases
InterProi | View protein in InterPro IPR006694, Fatty_acid_hydroxylase |
Pfami | View protein in Pfam PF04116, FA_hydroxylase, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MSMO1_MOUSE | |
Accessioni | Q9CRA4Primary (citable) accession number: Q9CRA4 Secondary accession number(s): Q543V8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 22, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families