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Protein

Methylsterol monooxygenase 1

Gene

Msmo1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.By similarity

Catalytic activityi

4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + 6 ferrocytochrome b5 + 3 O2 + 6 H+ = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + 6 ferricytochrome b5 + 4 H2O.By similarity

Cofactori

Fe cationBy similarity

Pathwayi: zymosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. Lanosterol 14-alpha demethylase (Cyp51a1)
  2. no protein annotated in this organism
  3. Methylsterol monooxygenase 1 (Msmo1)
  4. Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
  5. 3-keto-steroid reductase (Hsd17b7)
  6. no protein annotated in this organism
This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functioni

  • C-4 methylsterol oxidase activity Source: GO_Central
  • iron ion binding Source: InterPro

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
Biological processLipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
LigandIron, NAD

Enzyme and pathway databases

ReactomeiR-MMU-191273 Cholesterol biosynthesis
UniPathwayi
UPA00770;UER00756

Names & Taxonomyi

Protein namesi
Recommended name:
Methylsterol monooxygenase 1 (EC:1.14.18.9By similarity)
Alternative name(s):
C-4 methylsterol oxidase
Gene namesi
Name:Msmo1
Synonyms:Sc4mol
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 8

Organism-specific databases

MGIiMGI:1913484 Msmo1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei55 – 75HelicalSequence analysisAdd BLAST21
Transmembranei100 – 120HelicalSequence analysisAdd BLAST21
Transmembranei199 – 219HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001170341 – 293Methylsterol monooxygenase 1Add BLAST293

Proteomic databases

EPDiQ9CRA4
MaxQBiQ9CRA4
PaxDbiQ9CRA4
PeptideAtlasiQ9CRA4
PRIDEiQ9CRA4

PTM databases

iPTMnetiQ9CRA4
PhosphoSitePlusiQ9CRA4

Expressioni

Gene expression databases

BgeeiENSMUSG00000031604 Expressed in 320 organ(s), highest expression level in sciatic nerve
CleanExiMM_SC4MOL
ExpressionAtlasiQ9CRA4 baseline and differential
GenevisibleiQ9CRA4 MM

Interactioni

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034015

Structurei

3D structure databases

ProteinModelPortaliQ9CRA4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi157 – 161Histidine box-15
Motifi170 – 174Histidine box-25
Motifi249 – 255Histidine box-37

Domaini

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesi

Belongs to the sterol desaturase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0873 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00530000063017
HOGENOMiHOG000162289
HOVERGENiHBG051504
InParanoidiQ9CRA4
KOiK07750
OMAiWSGAEHH
OrthoDBiEOG091G0BM3
PhylomeDBiQ9CRA4
TreeFamiTF354294

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9CRA4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MATNKSVGVF SSASLAVEYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA
60 70 80 90 100
TWGSLIVHEA IYFLFSLPGF LFQFIPYMRK YKIQKDKPET FEGQWKCLKK
110 120 130 140 150
ILFNHFFIQL PLICGTYYFT EFFNIPYDWE RMPRWYLTLA RCLGCAVIED
160 170 180 190 200
TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF GIEAEYAHPL ETLILGTGFF
210 220 230 240 250
IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL VPFYTGARHH
260 270 280 290
DFHHMNFIGN YASTFTWWDK LFGTDAQYHA YIEKSKKLGK KSD
Length:293
Mass (Da):34,772
Last modified:June 1, 2001 - v1
Checksum:i0A275DE7B5D4DB52
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3Z458D3Z458_MOUSE
Methylsterol monooxygenase 1
Msmo1
109Annotation score:
D3YTN3D3YTN3_MOUSE
Methylsterol monooxygenase 1
Msmo1
76Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005090 mRNA Translation: BAB23811.1
AK005441 mRNA Translation: BAB24035.1
AK045059 mRNA Translation: BAC32201.1
AK146684 mRNA Translation: BAE27358.1
AK151135 mRNA Translation: BAE30143.1
AK169017 mRNA Translation: BAE40814.1
BC006802 mRNA Translation: AAH06802.1
CCDSiCCDS22328.1
RefSeqiNP_079712.1, NM_025436.2
UniGeneiMm.30119

Genome annotation databases

EnsembliENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604
GeneIDi66234
KEGGimmu:66234
UCSCiuc009lux.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK005090 mRNA Translation: BAB23811.1
AK005441 mRNA Translation: BAB24035.1
AK045059 mRNA Translation: BAC32201.1
AK146684 mRNA Translation: BAE27358.1
AK151135 mRNA Translation: BAE30143.1
AK169017 mRNA Translation: BAE40814.1
BC006802 mRNA Translation: AAH06802.1
CCDSiCCDS22328.1
RefSeqiNP_079712.1, NM_025436.2
UniGeneiMm.30119

3D structure databases

ProteinModelPortaliQ9CRA4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000034015

PTM databases

iPTMnetiQ9CRA4
PhosphoSitePlusiQ9CRA4

Proteomic databases

EPDiQ9CRA4
MaxQBiQ9CRA4
PaxDbiQ9CRA4
PeptideAtlasiQ9CRA4
PRIDEiQ9CRA4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604
GeneIDi66234
KEGGimmu:66234
UCSCiuc009lux.1 mouse

Organism-specific databases

CTDi6307
MGIiMGI:1913484 Msmo1

Phylogenomic databases

eggNOGiKOG0873 Eukaryota
COG3000 LUCA
GeneTreeiENSGT00530000063017
HOGENOMiHOG000162289
HOVERGENiHBG051504
InParanoidiQ9CRA4
KOiK07750
OMAiWSGAEHH
OrthoDBiEOG091G0BM3
PhylomeDBiQ9CRA4
TreeFamiTF354294

Enzyme and pathway databases

UniPathwayi
UPA00770;UER00756

ReactomeiR-MMU-191273 Cholesterol biosynthesis

Miscellaneous databases

ChiTaRSiMsmo1 mouse
PROiPR:Q9CRA4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031604 Expressed in 320 organ(s), highest expression level in sciatic nerve
CleanExiMM_SC4MOL
ExpressionAtlasiQ9CRA4 baseline and differential
GenevisibleiQ9CRA4 MM

Family and domain databases

InterProiView protein in InterPro
IPR006694 Fatty_acid_hydroxylase
PfamiView protein in Pfam
PF04116 FA_hydroxylase, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMSMO1_MOUSE
AccessioniPrimary (citable) accession number: Q9CRA4
Secondary accession number(s): Q543V8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 22, 2003
Last sequence update: June 1, 2001
Last modified: September 12, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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