UniProtKB - Q9CRA4 (MSMO1_MOUSE)

BLAST

>sp|Q9CRA4|MSMO1_MOUSE Methylsterol monooxygenase 1 OS=Mus musculus OX=10090 GN=Msmo1 PE=1 SV=1
MATNKSVGVFSSASLAVEYVDSLLPENPLQEPFKNAWVYMLDNYTKFQIATWGSLIVHEA
IYFLFSLPGFLFQFIPYMRKYKIQKDKPETFEGQWKCLKKILFNHFFIQLPLICGTYYFT
EFFNIPYDWERMPRWYLTLARCLGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPF
GIEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNL
VPFYTGARHHDFHHMNFIGNYASTFTWWDKLFGTDAQYHAYIEKSKKLGKKSD

Align
Format
Add to basket Added to basket
History
Entry version 124 (12 Sep 2018)
Sequence version 1 (01 Jun 2001)
Previous versions | rss
Other tutorials and videos
Help video
Feedback

Protein

Methylsterol monooxygenase 1

Gene

Msmo1

Organism

Mus musculus (Mouse)

Status

Reviewed-Annotation score: -Experimental evidence at protein levelⁱ

Functionⁱ

Catalyzes the first step in the removal of the two C-4 methyl groups of 4,4-dimethylzymosterol.By similarity

Catalytic activityⁱ

4,4-dimethyl-5-alpha-cholest-7-en-3-beta-ol + 6 ferrocytochrome b5 + 3 O₂ + 6 H⁺ = 3-beta-hydroxy-4-beta-methyl-5-alpha-cholest-7-ene-4-alpha-carboxylate + 6 ferricytochrome b5 + 4 H₂O.By similarity

Cofactorⁱ

Fe cationBy similarity

Pathwayⁱ: zymosterol biosynthesis

This protein is involved in step 3 of the subpathway that synthesizes zymosterol from lanosterol.
Proteins known to be involved in the 6 steps of the subpathway in this organism are:

Lanosterol 14-alpha demethylase (Cyp51a1)
no protein annotated in this organism
Methylsterol monooxygenase 1 (Msmo1)
Sterol-4-alpha-carboxylate 3-dehydrogenase, decarboxylating (Nsdhl)
3-keto-steroid reductase (Hsd17b7)
no protein annotated in this organism

This subpathway is part of the pathway zymosterol biosynthesis, which is itself part of Steroid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes zymosterol from lanosterol, the pathway zymosterol biosynthesis and in Steroid biosynthesis.

GO - Molecular functionⁱ

C-4 methylsterol oxidase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
iron ion binding Source: InterPro

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

sterol biosynthetic process
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis, Steroid metabolism, Sterol biosynthesis, Sterol metabolism
Ligand	Iron, NAD

Enzyme and pathway databases

Reactomeⁱ	R-MMU-191273 Cholesterol biosynthesis
UniPathwayⁱ	UPA00770;UER00756

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Methylsterol monooxygenase 1 (EC:1.14.18.9By similarity) Alternative name(s): C-4 methylsterol oxidase
Gene namesⁱ	Name:Msmo1 Synonyms:Sc4mol
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 8

Organism-specific databases

MGIⁱ	MGI:1913484 Msmo1

Topology

Feature key	Position(s)	DescriptionActions	Length
Transmembraneⁱ	55 – 75	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	100 – 120	HelicalSequence analysisAdd BLAST	21
Transmembraneⁱ	199 – 219	HelicalSequence analysisAdd BLAST	21

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000117034	1 – 293	Methylsterol monooxygenase 1Add BLAST		293

Proteomic databases

EPDⁱ	Q9CRA4
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9CRA4
PaxDbⁱ	Q9CRA4
PeptideAtlas More...PeptideAtlasⁱ	Q9CRA4
PRIDEⁱ	Q9CRA4

PTM databases

iPTMnetⁱ	Q9CRA4
PhosphoSitePlusⁱ	Q9CRA4

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000031604 Expressed in 320 organ(s), highest expression level in sciatic nerve
CleanExⁱ	MM_SC4MOL
ExpressionAtlasⁱ	Q9CRA4 baseline and differential
Genevisibleⁱ	Q9CRA4 MM

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000034015

STRINGⁱ

10090.ENSMUSP00000034015

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9CRA4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	157 – 161	Histidine box-1	5
Motifⁱ	170 – 174	Histidine box-2	5
Motifⁱ	249 – 255	Histidine box-3	7

Domainⁱ

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesⁱ

Belongs to the sterol desaturase family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG0873 Eukaryota COG3000 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000063017
HOGENOMⁱ	HOG000162289
HOVERGENⁱ	HBG051504
InParanoidⁱ	Q9CRA4
KEGG Orthology (KO) More...KOⁱ	K07750
OMAⁱ	WSGAEHH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0BM3
PhylomeDBⁱ	Q9CRA4
TreeFamⁱ	TF354294

Family and domain databases

InterProⁱ	View protein in InterPro IPR006694 Fatty_acid_hydroxylase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04116 FA_hydroxylase, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show all Align All

Q9CRA4-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MATNKSVGVF SSASLAVEYV DSLLPENPLQ EPFKNAWVYM LDNYTKFQIA 
        60         70         80         90        100
TWGSLIVHEA IYFLFSLPGF LFQFIPYMRK YKIQKDKPET FEGQWKCLKK 
       110        120        130        140        150
ILFNHFFIQL PLICGTYYFT EFFNIPYDWE RMPRWYLTLA RCLGCAVIED 
       160        170        180        190        200
TWHYFLHRLL HHKRIYKYIH KVHHEFQAPF GIEAEYAHPL ETLILGTGFF 
       210        220        230        240        250
IGIVLLCDHV ILLWAWVTIR LLETIDVHSG YDIPLNPLNL VPFYTGARHH 
       260        270        280        290 
DFHHMNFIGN YASTFTWWDK LFGTDAQYHA YIEKSKKLGK KSD

Length:293

Mass (Da):34,772

Last modified:June 1, 2001 - v1

Checksum:ⁱ0A275DE7B5D4DB52

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

Entry	Entry name	Protein names	Gene names	Length	Annotation

D3Z458	D3Z458_MOUSE	Methylsterol monooxygenase 1 Methylsterol monooxygenase 1	Msmo1	109	Annotation score:
D3YTN3	D3YTN3_MOUSE	Methylsterol monooxygenase 1 Methylsterol monooxygenase 1	Msmo1	76	Annotation score:

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK005090 mRNA Translation: BAB23811.1 AK005441 mRNA Translation: BAB24035.1 AK045059 mRNA Translation: BAC32201.1 AK146684 mRNA Translation: BAE27358.1 AK151135 mRNA Translation: BAE30143.1 AK169017 mRNA Translation: BAE40814.1 BC006802 mRNA Translation: AAH06802.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS22328.1
NCBI Reference Sequences More...RefSeqⁱ	NP_079712.1, NM_025436.2
UniGeneⁱ	Mm.30119

Genome annotation databases

Ensemblⁱ	ENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604
GeneIDⁱ	66234
KEGGⁱ	mmu:66234
UCSC genome browser More...UCSCⁱ	uc009lux.1 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9CRA4	Methylsterol monooxygenase 1 (Fragment)		MOUSE		76	UniRef100_Q9CRA4
Methylsterol monooxygenase 1 (Fragment)		MOUSE		109

Protein	Similar proteins		Species	Score	Length	Source
Q9CRA4	Methylsterol monooxygenase 1		RAT		293	UniRef90_O35532
Methylsterol monooxygenase 1 (Fragment)		MOUSE		76
C-4 methylsterol oxidase		CRIGR		292
UPI00038C2B3F		MICOH		293
UPI000A311845		MUSPA		293
+4

Protein	Similar proteins		Species	Score	Length	Source
Q9CRA4	Methylsterol monooxygenase 1		RAT		293	UniRef50_O35532
Methylsterol monooxygenase 1		DANRE		291
Methylsterol monooxygenase 1 (Fragment)		MOUSE		76
C-4 methylsterol oxidase		CRIGR		292
methylsterol monooxygenase 1-like		LEPWE		291
+121

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AK005090 mRNA Translation: BAB23811.1 AK005441 mRNA Translation: BAB24035.1 AK045059 mRNA Translation: BAC32201.1 AK146684 mRNA Translation: BAE27358.1 AK151135 mRNA Translation: BAE30143.1 AK169017 mRNA Translation: BAE40814.1 BC006802 mRNA Translation: AAH06802.1
CCDSⁱ	CCDS22328.1
RefSeqⁱ	NP_079712.1, NM_025436.2
UniGeneⁱ	Mm.30119

3D structure databases

ProteinModelPortalⁱ	Q9CRA4
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	10090.ENSMUSP00000034015

STRINGⁱ

10090.ENSMUSP00000034015

PTM databases

iPTMnetⁱ	Q9CRA4
PhosphoSitePlusⁱ	Q9CRA4

Proteomic databases

EPDⁱ	Q9CRA4
MaxQBⁱ	Q9CRA4
PaxDbⁱ	Q9CRA4
PeptideAtlasⁱ	Q9CRA4
PRIDEⁱ	Q9CRA4

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000034015; ENSMUSP00000034015; ENSMUSG00000031604
GeneIDⁱ	66234
KEGGⁱ	mmu:66234
UCSCⁱ	uc009lux.1 mouse

Organism-specific databases

CTDⁱ	6307
MGIⁱ	MGI:1913484 Msmo1

Phylogenomic databases

eggNOGⁱ	KOG0873 Eukaryota COG3000 LUCA
GeneTreeⁱ	ENSGT00530000063017
HOGENOMⁱ	HOG000162289
HOVERGENⁱ	HBG051504
InParanoidⁱ	Q9CRA4
KOⁱ	K07750
OMAⁱ	WSGAEHH
OrthoDBⁱ	EOG091G0BM3
PhylomeDBⁱ	Q9CRA4
TreeFamⁱ	TF354294

Enzyme and pathway databases

UniPathwayⁱ	UPA00770;UER00756
Reactomeⁱ	R-MMU-191273 Cholesterol biosynthesis

Miscellaneous databases

ChiTaRSⁱ	Msmo1 mouse
Protein Ontology More...PROⁱ	PR:Q9CRA4
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000031604 Expressed in 320 organ(s), highest expression level in sciatic nerve
CleanExⁱ	MM_SC4MOL
ExpressionAtlasⁱ	Q9CRA4 baseline and differential
Genevisibleⁱ	Q9CRA4 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR006694 Fatty_acid_hydroxylase
Pfamⁱ	View protein in Pfam PF04116 FA_hydroxylase, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	MSMO1_MOUSE
Accessionⁱ	Q9CRA4Primary (citable) accession number: Q9CRA4 Secondary accession number(s): Q543V8
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	February 22, 2003
	Last sequence update:	June 1, 2001
	Last modified:	September 12, 2018
	This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
PATHWAY comments
Index of metabolic and biosynthesis pathways

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9CRA4 (MSMO1_MOUSE)

Your basket is currently empty. i <p>When browsing through different UniProt proteins, you can use the ‘basket’ to save them, so that you can back to find or analyse them later.<p><a href='/help/basket' target='_top'>More...</a></p>

Methylsterol monooxygenase 1

Msmo1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: zymosterol biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

UniProt is an ELIXIR core data resource

Your basket is currently empty. ⁱ

Functionⁱ

Pathwayⁱ: zymosterol biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ