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Protein

WASH complex subunit 3

Gene

Washc3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acts at least in part as component of the WASH core complex whose assembly at the surface of endosomes seems to inhibit WASH nucleation-promoting factor (NPF) activity in recruiting and activating the Arp2/3 complex to induce actin polymerization, and which is involved in regulation of the fission of tubules that serve as transport intermediates during endosome sorting.By similarity

GO - Biological processi

  • actin filament polymerization Source: GO_Central
  • exocytosis Source: GO_Central
  • protein transport Source: UniProtKB-KW

Keywordsi

Biological processProtein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
WASH complex subunit 3Imported
Alternative name(s):
Coiled-coil domain-containing protein 53
Gene namesi
Name:Washc3Imported
Synonyms:Ccdc53
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 10

Organism-specific databases

MGIiMGI:1914532 Washc3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000762021 – 194WASH complex subunit 3Add BLAST194

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CR27
MaxQBiQ9CR27
PaxDbiQ9CR27
PeptideAtlasiQ9CR27
PRIDEiQ9CR27

PTM databases

iPTMnetiQ9CR27
PhosphoSitePlusiQ9CR27

Expressioni

Gene expression databases

BgeeiENSMUSG00000020056 Expressed in 292 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_CCDC53
ExpressionAtlasiQ9CR27 baseline and differential
GenevisibleiQ9CR27 MM

Interactioni

Subunit structurei

Component of the WASH core complex also described as WASH regulatory complex (SHRC) composed of WASHC1, WASHC2, WASHC3, WASHC4 and WASHC5. The WASH core complex associates via WASHC2 with the F-actin-capping protein dimer (formed by CAPZA1, CAPZA2 or CAPZA3 and CAPZB) in a transient or substoichiometric manner which was initially described as WASH complex.By similarity

Protein-protein interaction databases

ComplexPortaliCPX-1177 WASH complex, variant WASHC1/WASHC2
IntActiQ9CR27, 1 interactor
STRINGi10090.ENSMUSP00000020248

Structurei

3D structure databases

ProteinModelPortaliQ9CR27
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili46 – 74Sequence analysisAdd BLAST29

Sequence similaritiesi

Belongs to the CCDC53 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4496 Eukaryota
ENOG4111KKV LUCA
GeneTreeiENSGT00390000014084
HOGENOMiHOG000006526
HOVERGENiHBG009789
InParanoidiQ9CR27
KOiK18463
OrthoDBiEOG091G0Y6M
PhylomeDBiQ9CR27
TreeFamiTF318955

Family and domain databases

InterProiView protein in InterPro
IPR019309 WASHC3
PANTHERiPTHR13015 PTHR13015, 1 hit
PfamiView protein in Pfam
PF10152 CCDC53, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All

Q9CR27-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MDEDGLPLMG SGIDLTKVPA IQQKRTVAFL NQFVVHTVQF LNRFSAVCEE
60 70 80 90 100
KLADLSLRIQ QIETTLNILD AKLSSIPGLE DVTVEVSPLN VTAVTNGSHS
110 120 130 140 150
ETTSEQTQQN STQDSGAQES EAPSENVLTV AKDPRYARYL KMVQVGVPVM
160 170 180 190
AIRDKMISEG LDPELLEKPD APVPNGESER AVEESSDSDS SFSD
Length:194
Mass (Da):21,093
Last modified:June 1, 2001 - v1
Checksum:i2BC096A01AFE566E
GO

Computationally mapped potential isoform sequencesi

There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
S4R287S4R287_MOUSE
WASH complex subunit 3
Washc3 Ccdc53
193Annotation score:
G3UWG7G3UWG7_MOUSE
Coiled-coil domain containing 53, i...
Washc3 Ccdc53, mCG_16785
179Annotation score:
S4R1P7S4R1P7_MOUSE
WASH complex subunit 3
Washc3 Ccdc53
171Annotation score:
S4R1K7S4R1K7_MOUSE
WASH complex subunit 3
Washc3 Ccdc53
50Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti140L → R in AAH29256 (PubMed:15489334).Curated1
Sequence conflicti153R → G in BAB23220 (PubMed:16141072).Curated1
Sequence conflicti179E → K in AAH29256 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004206 mRNA Translation: BAB23220.1
AK009358 mRNA Translation: BAB26239.1
AK013835 mRNA Translation: BAB29011.1
AK158822 mRNA Translation: BAE34682.1
BC029256 mRNA Translation: AAH29256.1
CCDSiCCDS24108.1
RefSeqiNP_001116432.1, NM_001122960.1
NP_080346.1, NM_026070.3
NP_081763.1, NM_027487.1
UniGeneiMm.208465

Genome annotation databases

EnsembliENSMUST00000020248; ENSMUSP00000020248; ENSMUSG00000020056
GeneIDi67282
KEGGimmu:67282
UCSCiuc007grh.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004206 mRNA Translation: BAB23220.1
AK009358 mRNA Translation: BAB26239.1
AK013835 mRNA Translation: BAB29011.1
AK158822 mRNA Translation: BAE34682.1
BC029256 mRNA Translation: AAH29256.1
CCDSiCCDS24108.1
RefSeqiNP_001116432.1, NM_001122960.1
NP_080346.1, NM_026070.3
NP_081763.1, NM_027487.1
UniGeneiMm.208465

3D structure databases

ProteinModelPortaliQ9CR27
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

ComplexPortaliCPX-1177 WASH complex, variant WASHC1/WASHC2
IntActiQ9CR27, 1 interactor
STRINGi10090.ENSMUSP00000020248

PTM databases

iPTMnetiQ9CR27
PhosphoSitePlusiQ9CR27

Proteomic databases

EPDiQ9CR27
MaxQBiQ9CR27
PaxDbiQ9CR27
PeptideAtlasiQ9CR27
PRIDEiQ9CR27

Protocols and materials databases

DNASUi67282
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000020248; ENSMUSP00000020248; ENSMUSG00000020056
GeneIDi67282
KEGGimmu:67282
UCSCiuc007grh.2 mouse

Organism-specific databases

CTDi51019
MGIiMGI:1914532 Washc3

Phylogenomic databases

eggNOGiKOG4496 Eukaryota
ENOG4111KKV LUCA
GeneTreeiENSGT00390000014084
HOGENOMiHOG000006526
HOVERGENiHBG009789
InParanoidiQ9CR27
KOiK18463
OrthoDBiEOG091G0Y6M
PhylomeDBiQ9CR27
TreeFamiTF318955

Miscellaneous databases

ChiTaRSiCcdc53 mouse
PROiPR:Q9CR27
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000020056 Expressed in 292 organ(s), highest expression level in lens of camera-type eye
CleanExiMM_CCDC53
ExpressionAtlasiQ9CR27 baseline and differential
GenevisibleiQ9CR27 MM

Family and domain databases

InterProiView protein in InterPro
IPR019309 WASHC3
PANTHERiPTHR13015 PTHR13015, 1 hit
PfamiView protein in Pfam
PF10152 CCDC53, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWASC3_MOUSE
AccessioniPrimary (citable) accession number: Q9CR27
Secondary accession number(s): Q8K2X5, Q9D0Y7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 24, 2006
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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