Atg12

Functionⁱ

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.3 Publications

(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection.1 Publication

GO - Molecular functionⁱ

Atg8 ligase activity
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

autophagosome assembly
Source: UniProtKB
Inferred from mutant phenotypeⁱ
- 11266458
autophagy Source: MGI
autophagy of mitochondrion
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
C-terminal protein lipidation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Biological process	Autophagy, Ubl conjugation pathway

Biological process

Autophagy, Ubl conjugation pathway

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1632852 Macroautophagy R-MMU-5205685 Pink/Parkin Mediated Mitophagy R-MMU-8934903 Receptor Mediated Mitophagy

Reactomeⁱ

R-MMU-1632852 Macroautophagy
R-MMU-5205685 Pink/Parkin Mediated Mitophagy
R-MMU-8934903 Receptor Mediated Mitophagy

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Ubiquitin-like protein ATG12 Alternative name(s): Autophagy-related protein 12 Short name: APG12-like
Gene namesⁱ	Name:Atg12 Synonyms:Apg12, Apg12l
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 18

Organism-specific databases

MGIⁱ	MGI:1914776 Atg12

Keywords - Cellular componentⁱ

Cytoplasm, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000212472	1 – 141	Ubiquitin-like protein ATG12Add BLAST		141

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Cross-linkⁱ	141	Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)

Post-translational modificationⁱ

Acetylated by EP300.By similarity

Keywords - PTMⁱ

Acetylation, Isopeptide bond

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9CQY1
PaxDbⁱ	Q9CQY1
PeptideAtlas More...PeptideAtlasⁱ	Q9CQY1
PRIDEⁱ	Q9CQY1

PTM databases

iPTMnetⁱ	Q9CQY1
PhosphoSitePlusⁱ	Q9CQY1

Expressionⁱ

Tissue specificityⁱ

Ubiquitous.

Gene expression databases

Bgeeⁱ	ENSMUSG00000032905 Expressed in 293 organ(s), highest expression level in blood
CleanExⁱ	MM_ATG12
Genevisibleⁱ	Q9CQY1 MM

Interactionⁱ

Subunit structureⁱ

Forms a conjugate with ATG5 (PubMed:11266458, PubMed:12482611, PubMed:12890687, PubMed:12665549, PubMed:18768753, PubMed:19417210). The ATG12-ATG5 conjugate forms a complex with several units of ATG16L (PubMed:12665549). Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection. Interacts with ATG3 and ATG7 (By similarity). Interacts with ATG10 (PubMed:12482611). Interacts with TECPR1 (By similarity).By similarity6 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
Atg3	Q9CPX6	5	EBI-2911788,EBI-2911810
Atg5	Q99J83	4	EBI-2911788,EBI-2911848
Pdcd6ip	Q9WU78-1	3	EBI-2911788,EBI-15788421

Protein-protein interaction databases

BioGridⁱ	212249, 4 interactors
ComplexPortalⁱ	CPX-328 Atg12-Atg5-Atg16l1 complex CPX-355 Atg12-Atg5-Atg16l2 complex CPX-357 Atg5-Atg12 complex CPX-360 ATG5-ATG12-TECPR1 complex
CORUMⁱ	Q9CQY1
Database of interacting proteins More...DIPⁱ	DIP-57729N
IntActⁱ	Q9CQY1, 10 interactors
Molecular INTeraction database More...MINTⁱ	Q9CQY1
STRINGⁱ	10090.ENSMUSP00000038489

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9CQY1
SMRⁱ	Q9CQY1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domainⁱ

Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.1 Publication

Sequence similaritiesⁱ

Belongs to the ATG12 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG3439 Eukaryota ENOG4111ND7 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000016654
HOGENOMⁱ	HOG000234863
HOVERGENⁱ	HBG080875
InParanoidⁱ	Q9CQY1
KEGG Orthology (KO) More...KOⁱ	K08336
OMAⁱ	IHYCKSQ
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0XR8
PhylomeDBⁱ	Q9CQY1
TreeFamⁱ	TF325131

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd01612 APG12_C, 1 hit
InterProⁱ	View protein in InterPro IPR007242 Atg12 IPR029071 Ubiquitin-like_domsf
PANTHERⁱ	PTHR13385 PTHR13385, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF04110 APG12, 1 hit
SUPFAMⁱ	SSF54236 SSF54236, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9CQY1-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MSEDSEVVLQ LPSAPVGAGG ESLPELSPET ATPEPPSSAA VSPGTEEPPG 
        60         70         80         90        100
DTKKKIDILL KAVGDTPIMK TKKWAVERTR TIQGLIDFIK KFLKLVASEQ 
       110        120        130        140 
LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G

Length:141

Mass (Da):15,207

Last modified:June 1, 2001 - v1

Checksum:ⁱ440F9A625624A78B

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	44 – 48	GTEEP → ERGT in BAB25839 (PubMed:16141072).Curated		5

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB066216 mRNA Translation: BAB62092.1 AK008698 mRNA Translation: BAB25839.1 AK016474 mRNA Translation: BAB30256.1 AK005405 mRNA Translation: BAB24005.1 AK167027 mRNA Translation: BAE39200.1 BC070470 mRNA Translation: AAH70470.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS37811.1
NCBI Reference Sequences More...RefSeqⁱ	NP_080493.2, NM_026217.3
UniGeneⁱ	Mm.9852

Genome annotation databases

Ensemblⁱ	ENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905
GeneIDⁱ	67526
KEGGⁱ	mmu:67526
UCSC genome browser More...UCSCⁱ	uc008evu.1 mouse

Similar proteinsⁱ

50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9CQY1	Ubiquitin-like protein ATG12		RAT		141	UniRef50_Q9CQY1
Ubiquitin-like protein ATG12		AMAAE		143
Ubiquitin-like protein ATG12		Patagioenas fasciata monilis		142
Ubiquitin-like protein ATG12		TAEGU		143
Ubiquitin-like protein ATG12		Bengalese finch		73
+53

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB066216 mRNA Translation: BAB62092.1 AK008698 mRNA Translation: BAB25839.1 AK016474 mRNA Translation: BAB30256.1 AK005405 mRNA Translation: BAB24005.1 AK167027 mRNA Translation: BAE39200.1 BC070470 mRNA Translation: AAH70470.1
CCDSⁱ	CCDS37811.1
RefSeqⁱ	NP_080493.2, NM_026217.3
UniGeneⁱ	Mm.9852

3D structure databases

ProteinModelPortalⁱ	Q9CQY1
SMRⁱ	Q9CQY1
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	212249, 4 interactors
ComplexPortalⁱ	CPX-328 Atg12-Atg5-Atg16l1 complex CPX-355 Atg12-Atg5-Atg16l2 complex CPX-357 Atg5-Atg12 complex CPX-360 ATG5-ATG12-TECPR1 complex
CORUMⁱ	Q9CQY1
DIPⁱ	DIP-57729N
IntActⁱ	Q9CQY1, 10 interactors
MINTⁱ	Q9CQY1
STRINGⁱ	10090.ENSMUSP00000038489

PTM databases

iPTMnetⁱ	Q9CQY1
PhosphoSitePlusⁱ	Q9CQY1

Proteomic databases

MaxQBⁱ	Q9CQY1
PaxDbⁱ	Q9CQY1
PeptideAtlasⁱ	Q9CQY1
PRIDEⁱ	Q9CQY1

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905
GeneIDⁱ	67526
KEGGⁱ	mmu:67526
UCSCⁱ	uc008evu.1 mouse

Organism-specific databases

CTDⁱ	9140
MGIⁱ	MGI:1914776 Atg12

Phylogenomic databases

eggNOGⁱ	KOG3439 Eukaryota ENOG4111ND7 LUCA
GeneTreeⁱ	ENSGT00390000016654
HOGENOMⁱ	HOG000234863
HOVERGENⁱ	HBG080875
InParanoidⁱ	Q9CQY1
KOⁱ	K08336
OMAⁱ	IHYCKSQ
OrthoDBⁱ	EOG091G0XR8
PhylomeDBⁱ	Q9CQY1
TreeFamⁱ	TF325131

Enzyme and pathway databases

Reactomeⁱ	R-MMU-1632852 Macroautophagy R-MMU-5205685 Pink/Parkin Mediated Mitophagy R-MMU-8934903 Receptor Mediated Mitophagy

Reactomeⁱ

R-MMU-1632852 Macroautophagy
R-MMU-5205685 Pink/Parkin Mediated Mitophagy
R-MMU-8934903 Receptor Mediated Mitophagy

Miscellaneous databases

ChiTaRSⁱ	Atg12 mouse
Protein Ontology More...PROⁱ	PR:Q9CQY1
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000032905 Expressed in 293 organ(s), highest expression level in blood
CleanExⁱ	MM_ATG12
Genevisibleⁱ	Q9CQY1 MM

Family and domain databases

CDDⁱ	cd01612 APG12_C, 1 hit
InterProⁱ	View protein in InterPro IPR007242 Atg12 IPR029071 Ubiquitin-like_domsf
PANTHERⁱ	PTHR13385 PTHR13385, 1 hit
Pfamⁱ	View protein in Pfam PF04110 APG12, 1 hit
SUPFAMⁱ	SSF54236 SSF54236, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ATG12_MOUSE
Accessionⁱ	Q9CQY1Primary (citable) accession number: Q9CQY1 Secondary accession number(s): Q3TKE5, Q9D7Y5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 16, 2001
	Last sequence update:	June 1, 2001
	Last modified:	November 7, 2018
	This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - Q9CQY1 (ATG12_MOUSE)

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Ubiquitin-like protein ATG12

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Other locations

Cytosol

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

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Keywords - Technical termi

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ