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Protein

Ubiquitin-like protein ATG12

Gene

Atg12

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-like protein involved in autophagy vesicles formation. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate acts as an E3-like enzyme which is required for lipidation of ATG8 family proteins and their association to the vesicle membranes.3 Publications
(Microbial infection) May act as a proviral factor. In association with ATG5, negatively regulates the innate antiviral immune response by impairing the type I IFN production pathway upon vesicular stomatitis virus (VSV) infection.1 Publication

GO - Molecular functioni

  • Atg8 ligase activity Source: GO_Central

GO - Biological processi

  • autophagosome assembly Source: UniProtKB
  • autophagy Source: MGI
  • autophagy of mitochondrion Source: GO_Central
  • C-terminal protein lipidation Source: GO_Central

Keywordsi

Biological processAutophagy, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-5205685 Pink/Parkin Mediated Mitophagy
R-MMU-8934903 Receptor Mediated Mitophagy

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-like protein ATG12
Alternative name(s):
Autophagy-related protein 12
Short name:
APG12-like
Gene namesi
Name:Atg12
Synonyms:Apg12, Apg12l
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 18

Organism-specific databases

MGIiMGI:1914776 Atg12

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002124721 – 141Ubiquitin-like protein ATG12Add BLAST141

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki141Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein)

Post-translational modificationi

Acetylated by EP300.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond

Proteomic databases

MaxQBiQ9CQY1
PaxDbiQ9CQY1
PeptideAtlasiQ9CQY1
PRIDEiQ9CQY1

PTM databases

iPTMnetiQ9CQY1
PhosphoSitePlusiQ9CQY1

Expressioni

Tissue specificityi

Ubiquitous.

Gene expression databases

BgeeiENSMUSG00000032905 Expressed in 293 organ(s), highest expression level in blood
CleanExiMM_ATG12
GenevisibleiQ9CQY1 MM

Interactioni

Subunit structurei

Forms a conjugate with ATG5 (PubMed:11266458, PubMed:12482611, PubMed:12890687, PubMed:12665549, PubMed:18768753, PubMed:19417210). The ATG12-ATG5 conjugate forms a complex with several units of ATG16L (PubMed:12665549). Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection. Interacts with ATG3 and ATG7 (By similarity). Interacts with ATG10 (PubMed:12482611). Interacts with TECPR1 (By similarity).By similarity6 Publications

Binary interactionsi

Protein-protein interaction databases

BioGridi212249, 4 interactors
ComplexPortaliCPX-328 Atg12-Atg5-Atg16l1 complex
CPX-355 Atg12-Atg5-Atg16l2 complex
CPX-357 Atg5-Atg12 complex
CPX-360 ATG5-ATG12-TECPR1 complex
CORUMiQ9CQY1
DIPiDIP-57729N
IntActiQ9CQY1, 10 interactors
MINTiQ9CQY1
STRINGi10090.ENSMUSP00000038489

Structurei

3D structure databases

ProteinModelPortaliQ9CQY1
SMRiQ9CQY1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domaini

Shares weak sequence similarity with ubiquitin family, but contains an 'ubiquitin superfold' and the C-terminal Gly is required for isopeptide linkage.1 Publication

Sequence similaritiesi

Belongs to the ATG12 family.Curated

Phylogenomic databases

eggNOGiKOG3439 Eukaryota
ENOG4111ND7 LUCA
GeneTreeiENSGT00390000016654
HOGENOMiHOG000234863
HOVERGENiHBG080875
InParanoidiQ9CQY1
KOiK08336
OMAiIHYCKSQ
OrthoDBiEOG091G0XR8
PhylomeDBiQ9CQY1
TreeFamiTF325131

Family and domain databases

CDDicd01612 APG12_C, 1 hit
InterProiView protein in InterPro
IPR007242 Atg12
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR13385 PTHR13385, 1 hit
PfamiView protein in Pfam
PF04110 APG12, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CQY1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSEDSEVVLQ LPSAPVGAGG ESLPELSPET ATPEPPSSAA VSPGTEEPPG
60 70 80 90 100
DTKKKIDILL KAVGDTPIMK TKKWAVERTR TIQGLIDFIK KFLKLVASEQ
110 120 130 140
LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G
Length:141
Mass (Da):15,207
Last modified:June 1, 2001 - v1
Checksum:i440F9A625624A78B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti44 – 48GTEEP → ERGT in BAB25839 (PubMed:16141072).Curated5

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB066216 mRNA Translation: BAB62092.1
AK008698 mRNA Translation: BAB25839.1
AK016474 mRNA Translation: BAB30256.1
AK005405 mRNA Translation: BAB24005.1
AK167027 mRNA Translation: BAE39200.1
BC070470 mRNA Translation: AAH70470.1
CCDSiCCDS37811.1
RefSeqiNP_080493.2, NM_026217.3
UniGeneiMm.9852

Genome annotation databases

EnsembliENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905
GeneIDi67526
KEGGimmu:67526
UCSCiuc008evu.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB066216 mRNA Translation: BAB62092.1
AK008698 mRNA Translation: BAB25839.1
AK016474 mRNA Translation: BAB30256.1
AK005405 mRNA Translation: BAB24005.1
AK167027 mRNA Translation: BAE39200.1
BC070470 mRNA Translation: AAH70470.1
CCDSiCCDS37811.1
RefSeqiNP_080493.2, NM_026217.3
UniGeneiMm.9852

3D structure databases

ProteinModelPortaliQ9CQY1
SMRiQ9CQY1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212249, 4 interactors
ComplexPortaliCPX-328 Atg12-Atg5-Atg16l1 complex
CPX-355 Atg12-Atg5-Atg16l2 complex
CPX-357 Atg5-Atg12 complex
CPX-360 ATG5-ATG12-TECPR1 complex
CORUMiQ9CQY1
DIPiDIP-57729N
IntActiQ9CQY1, 10 interactors
MINTiQ9CQY1
STRINGi10090.ENSMUSP00000038489

PTM databases

iPTMnetiQ9CQY1
PhosphoSitePlusiQ9CQY1

Proteomic databases

MaxQBiQ9CQY1
PaxDbiQ9CQY1
PeptideAtlasiQ9CQY1
PRIDEiQ9CQY1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905
GeneIDi67526
KEGGimmu:67526
UCSCiuc008evu.1 mouse

Organism-specific databases

CTDi9140
MGIiMGI:1914776 Atg12

Phylogenomic databases

eggNOGiKOG3439 Eukaryota
ENOG4111ND7 LUCA
GeneTreeiENSGT00390000016654
HOGENOMiHOG000234863
HOVERGENiHBG080875
InParanoidiQ9CQY1
KOiK08336
OMAiIHYCKSQ
OrthoDBiEOG091G0XR8
PhylomeDBiQ9CQY1
TreeFamiTF325131

Enzyme and pathway databases

ReactomeiR-MMU-1632852 Macroautophagy
R-MMU-5205685 Pink/Parkin Mediated Mitophagy
R-MMU-8934903 Receptor Mediated Mitophagy

Miscellaneous databases

ChiTaRSiAtg12 mouse
PROiPR:Q9CQY1
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032905 Expressed in 293 organ(s), highest expression level in blood
CleanExiMM_ATG12
GenevisibleiQ9CQY1 MM

Family and domain databases

CDDicd01612 APG12_C, 1 hit
InterProiView protein in InterPro
IPR007242 Atg12
IPR029071 Ubiquitin-like_domsf
PANTHERiPTHR13385 PTHR13385, 1 hit
PfamiView protein in Pfam
PF04110 APG12, 1 hit
SUPFAMiSSF54236 SSF54236, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiATG12_MOUSE
AccessioniPrimary (citable) accession number: Q9CQY1
Secondary accession number(s): Q3TKE5, Q9D7Y5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 16, 2001
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 142 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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