UniProtKB - Q9CQY1 (ATG12_MOUSE)
Ubiquitin-like protein ATG12
Atg12
Functioni
GO - Biological processi
- autophagosome assembly Source: UniProtKB
- autophagy Source: MGI
- autophagy of mitochondrion Source: GO_Central
- autophagy of nucleus Source: GO_Central
- C-terminal protein lipidation Source: GO_Central
Keywordsi
Biological process | Autophagy, Ubl conjugation pathway |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-5205685, PINK1-PRKN Mediated Mitophagy R-MMU-8934903, Receptor Mediated Mitophagy |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin-like protein ATG12Alternative name(s): Autophagy-related protein 12 Short name: APG12-like |
Gene namesi | Name:Atg12 Synonyms:Apg12, Apg12l |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914776, Atg12 |
Subcellular locationi
Other locations
- Cytoplasm By similarity
- Preautophagosomal structure membrane By similarity; Peripheral membrane protein By similarity
Note: TECPR1 recruits the ATG12-ATG5 conjugate to the autolysosomal membrane.By similarity
Cytosol
- cytosol Source: Reactome
Other locations
- Atg12-Atg5-Atg16 complex Source: GO_Central
- autophagosome Source: MGI
- membrane Source: ParkinsonsUK-UCL
- phagophore assembly site membrane Source: UniProtKB
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000212472 | 1 – 141 | Ubiquitin-like protein ATG12Add BLAST | 141 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 141 | Glycyl lysine isopeptide (Gly-Lys) (interchain with K-? in acceptor protein) |
Post-translational modificationi
Keywords - PTMi
Acetylation, Isopeptide bondProteomic databases
MaxQBi | Q9CQY1 |
PaxDbi | Q9CQY1 |
PeptideAtlasi | Q9CQY1 |
PRIDEi | Q9CQY1 |
PTM databases
iPTMneti | Q9CQY1 |
PhosphoSitePlusi | Q9CQY1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000032905, Expressed in blood and 310 other tissues |
Genevisiblei | Q9CQY1, MM |
Interactioni
Subunit structurei
Forms a conjugate with ATG5 (PubMed:11266458, PubMed:12482611, PubMed:12890687, PubMed:12665549, PubMed:18768753, PubMed:19417210). The ATG12-ATG5 conjugate forms a complex with several units of ATG16L1 (PubMed:12665549).
Forms an 800-kDa complex composed of ATG12-ATG5 and ATG16L2 (PubMed:22082872).
Interacts with DHX58/RIG-1, IFIH1/MDA5 and MAVS/IPS-1 in monomeric form as well as in ATG12-ATG5 conjugate. The interaction with MAVS is further enhanced upon vesicular stomatitis virus (VSV) infection.
Interacts with ATG3 and ATG7 (By similarity).
Interacts with ATG10 (PubMed:12482611).
Interacts with TECPR1 (By similarity).
By similarity7 PublicationsBinary interactionsi
Hide detailsQ9CQY1
With | #Exp. | IntAct |
---|---|---|
Atg3 [Q9CPX6] | 5 | EBI-2911788,EBI-2911810 |
Atg5 [Q99J83] | 4 | EBI-2911788,EBI-2911848 |
Pdcd6ip - isoform 1 [Q9WU78-1] | 3 | EBI-2911788,EBI-15788421 |
Protein-protein interaction databases
BioGRIDi | 212249, 17 interactors |
ComplexPortali | CPX-328, Atg12-Atg5-Atg16l1 complex CPX-355, Atg12-Atg5-Atg16l2 complex CPX-357, Atg5-Atg12 complex CPX-360, ATG5-ATG12-TECPR1 complex |
CORUMi | Q9CQY1 |
DIPi | DIP-57729N |
IntActi | Q9CQY1, 10 interactors |
MINTi | Q9CQY1 |
STRINGi | 10090.ENSMUSP00000038489 |
Miscellaneous databases
RNActi | Q9CQY1, protein |
Family & Domainsi
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3439, Eukaryota |
GeneTreei | ENSGT00390000016654 |
HOGENOMi | CLU_106795_3_0_1 |
InParanoidi | Q9CQY1 |
OMAi | GNLWRCF |
OrthoDBi | 1525971at2759 |
PhylomeDBi | Q9CQY1 |
TreeFami | TF325131 |
Family and domain databases
InterProi | View protein in InterPro IPR007242, Atg12 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR13385, PTHR13385, 1 hit |
Pfami | View protein in Pfam PF04110, APG12, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MSEDSEVVLQ LPSAPVGAGG ESLPELSPET ATPEPPSSAA VSPGTEEPPG
60 70 80 90 100
DTKKKIDILL KAVGDTPIMK TKKWAVERTR TIQGLIDFIK KFLKLVASEQ
110 120 130 140
LFIYVNQSFA PSPDQEVGTL YECFGSDGKL VLHYCKSQAW G
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 44 – 48 | GTEEP → ERGT in BAB25839 (PubMed:16141072).Curated | 5 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB066216 mRNA Translation: BAB62092.1 AK008698 mRNA Translation: BAB25839.1 AK016474 mRNA Translation: BAB30256.1 AK005405 mRNA Translation: BAB24005.1 AK167027 mRNA Translation: BAE39200.1 BC070470 mRNA Translation: AAH70470.1 |
CCDSi | CCDS37811.1 |
RefSeqi | NP_080493.2, NM_026217.3 |
Genome annotation databases
Ensembli | ENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905 |
GeneIDi | 67526 |
KEGGi | mmu:67526 |
UCSCi | uc008evu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB066216 mRNA Translation: BAB62092.1 AK008698 mRNA Translation: BAB25839.1 AK016474 mRNA Translation: BAB30256.1 AK005405 mRNA Translation: BAB24005.1 AK167027 mRNA Translation: BAE39200.1 BC070470 mRNA Translation: AAH70470.1 |
CCDSi | CCDS37811.1 |
RefSeqi | NP_080493.2, NM_026217.3 |
3D structure databases
SMRi | Q9CQY1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212249, 17 interactors |
ComplexPortali | CPX-328, Atg12-Atg5-Atg16l1 complex CPX-355, Atg12-Atg5-Atg16l2 complex CPX-357, Atg5-Atg12 complex CPX-360, ATG5-ATG12-TECPR1 complex |
CORUMi | Q9CQY1 |
DIPi | DIP-57729N |
IntActi | Q9CQY1, 10 interactors |
MINTi | Q9CQY1 |
STRINGi | 10090.ENSMUSP00000038489 |
PTM databases
iPTMneti | Q9CQY1 |
PhosphoSitePlusi | Q9CQY1 |
Proteomic databases
MaxQBi | Q9CQY1 |
PaxDbi | Q9CQY1 |
PeptideAtlasi | Q9CQY1 |
PRIDEi | Q9CQY1 |
Protocols and materials databases
Antibodypediai | 4593, 768 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000035648; ENSMUSP00000038489; ENSMUSG00000032905 |
GeneIDi | 67526 |
KEGGi | mmu:67526 |
UCSCi | uc008evu.1, mouse |
Organism-specific databases
CTDi | 9140 |
MGIi | MGI:1914776, Atg12 |
Phylogenomic databases
eggNOGi | KOG3439, Eukaryota |
GeneTreei | ENSGT00390000016654 |
HOGENOMi | CLU_106795_3_0_1 |
InParanoidi | Q9CQY1 |
OMAi | GNLWRCF |
OrthoDBi | 1525971at2759 |
PhylomeDBi | Q9CQY1 |
TreeFami | TF325131 |
Enzyme and pathway databases
Reactomei | R-MMU-1632852, Macroautophagy R-MMU-5205685, PINK1-PRKN Mediated Mitophagy R-MMU-8934903, Receptor Mediated Mitophagy |
Miscellaneous databases
BioGRID-ORCSi | 67526, 7 hits in 23 CRISPR screens |
ChiTaRSi | Atg12, mouse |
PROi | PR:Q9CQY1 |
RNActi | Q9CQY1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032905, Expressed in blood and 310 other tissues |
Genevisiblei | Q9CQY1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR007242, Atg12 IPR029071, Ubiquitin-like_domsf |
PANTHERi | PTHR13385, PTHR13385, 1 hit |
Pfami | View protein in Pfam PF04110, APG12, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ATG12_MOUSE | |
Accessioni | Q9CQY1Primary (citable) accession number: Q9CQY1 Secondary accession number(s): Q3TKE5, Q9D7Y5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 16, 2001 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families