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Protein

PCNA-associated factor

Gene

Pclaf

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

PCNA-binding protein that acts as a regulator of DNA repair during DNA replication. Following DNA damage, the interaction with PCNA is disrupted, facilitating the interaction between monoubiquitinated PCNA and the translesion DNA synthesis DNA polymerase eta (POLH) at stalled replisomes, facilitating the bypass of replication-fork-blocking lesions. Also acts as a regulator of centrosome number (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Biological processDNA damage, DNA repair

Enzyme and pathway databases

ReactomeiR-MMU-5656169 Termination of translesion DNA synthesis

Names & Taxonomyi

Protein namesi
Recommended name:
PCNA-associated factorCurated
Alternative name(s):
HCV NS5A-transactivated protein 9 homolog
PCNA-associated factor of 15 kDa
Short name:
PAF15
Short name:
p15PAF
PCNA-clamp-associated factorBy similarity
Gene namesi
Name:PclafBy similarity
Synonyms:Ns5atp9, Paf
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1915276 Pclaf

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000966851 – 110PCNA-associated factorAdd BLAST110

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Cross-linki15Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei24N6-acetyllysine; alternateCombined sources1
Cross-linki24Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei28PhosphoserineBy similarity1
Modified residuei71PhosphoserineBy similarity1

Post-translational modificationi

Monoubiquitinated at Lys-15 and Lys-24 during normal S phase, promoting its association with PCNA. Also diubiquitinated at these 2 sites. Following DNA damage, monoubiquitin chains at Lys-15 and Lys-24 are probably extended, leading to disrupt the interaction with PCNA. Polyubiquitinated by the APC/C complex at the mitotic exit, leading to its degradation by the proteasome (By similarity).By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9CQX4
MaxQBiQ9CQX4
PaxDbiQ9CQX4
PeptideAtlasiQ9CQX4
PRIDEiQ9CQX4

PTM databases

iPTMnetiQ9CQX4
PhosphoSitePlusiQ9CQX4

Expressioni

Gene expression databases

BgeeiENSMUSG00000040204 Expressed in 172 organ(s), highest expression level in thymus
CleanExiMM_2810417H13RIK
GenevisibleiQ9CQX4 MM

Interactioni

Subunit structurei

Interacts (when monoubiquitinated at Lys-15 and Lys-24) with PCNA. Interacts with isoform 2/p33ING1b of ING1. Interacts with BRCA1 (By similarity).By similarity

Protein-protein interaction databases

IntActiQ9CQX4, 1 interactor
MINTiQ9CQX4
STRINGi10090.ENSMUSP00000038877

Structurei

3D structure databases

SMRiQ9CQX4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi23 – 34D-boxAdd BLAST12
Motifi61 – 71PIP-boxAdd BLAST11
Motifi77 – 79KEN box3
Motifi84 – 96Initiation motifAdd BLAST13

Domaini

The PIP-box mediates the interaction with PCNA.By similarity
The KEN box is required for the association with the APC/C complex.By similarity
The D-box (destruction box) mediates the interaction with APC/C proteins, and acts as a recognition signal for degradation via the ubiquitin-proteasome pathway.By similarity
The initiation motif is required for efficient chain initiation by the APC/C complex E2 ligase UBE2C. It determines the rate of substrate's degradation without affecting its affinity for the APC/C, a mechanism used by the APC/C to control the timing of substrate proteolysis during the cell cycle (By similarity).By similarity

Phylogenomic databases

eggNOGiENOG410J101 Eukaryota
ENOG410Y3KP LUCA
GeneTreeiENSGT00510000048252
HOGENOMiHOG000013069
HOVERGENiHBG052567
InParanoidiQ9CQX4
OMAiQPDHSDD
OrthoDBiEOG091G13N6
PhylomeDBiQ9CQX4
TreeFamiTF333199

Family and domain databases

InterProiView protein in InterPro
IPR031444 PCNA-AF
PANTHERiPTHR15679 PTHR15679, 1 hit
PfamiView protein in Pfam
PF15715 PAF, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CQX4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVRTKANYVP GAYRKAVASQ APRKVLGSST FVTNSSSSSR KAENKYAGGN
60 70 80 90 100
PVCVRPTPKW QKGIGEFFRL SPKESKKENQ APEEAGTSGL GKAKRKACPL
110
QPDHRDDENE
Length:110
Mass (Da):11,993
Last modified:June 1, 2001 - v1
Checksum:i47054C3F288B5BE3
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti40R → G in BAB28650 (PubMed:16141072).Curated1
Sequence conflicti51P → Q in AK011645 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496943 mRNA Translation: AAR90858.1
AK011090 mRNA Translation: BAB27391.1
AK011645 mRNA No translation available.
AK011835 mRNA Translation: BAB27868.1
AK013105 mRNA Translation: BAB28650.1
AK017673 mRNA Translation: BAB30866.1
BC021413 mRNA Translation: AAH21413.1
BC096393 mRNA Translation: AAH96393.1
CCDSiCCDS23299.1
RefSeqiNP_080791.2, NM_026515.2
UniGeneiMm.351273
Mm.460895

Genome annotation databases

EnsembliENSMUST00000045802; ENSMUSP00000038877; ENSMUSG00000040204
GeneIDi68026
KEGGimmu:68026
UCSCiuc009qeb.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY496943 mRNA Translation: AAR90858.1
AK011090 mRNA Translation: BAB27391.1
AK011645 mRNA No translation available.
AK011835 mRNA Translation: BAB27868.1
AK013105 mRNA Translation: BAB28650.1
AK017673 mRNA Translation: BAB30866.1
BC021413 mRNA Translation: AAH21413.1
BC096393 mRNA Translation: AAH96393.1
CCDSiCCDS23299.1
RefSeqiNP_080791.2, NM_026515.2
UniGeneiMm.351273
Mm.460895

3D structure databases

SMRiQ9CQX4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CQX4, 1 interactor
MINTiQ9CQX4
STRINGi10090.ENSMUSP00000038877

PTM databases

iPTMnetiQ9CQX4
PhosphoSitePlusiQ9CQX4

Proteomic databases

EPDiQ9CQX4
MaxQBiQ9CQX4
PaxDbiQ9CQX4
PeptideAtlasiQ9CQX4
PRIDEiQ9CQX4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000045802; ENSMUSP00000038877; ENSMUSG00000040204
GeneIDi68026
KEGGimmu:68026
UCSCiuc009qeb.1 mouse

Organism-specific databases

CTDi9768
MGIiMGI:1915276 Pclaf

Phylogenomic databases

eggNOGiENOG410J101 Eukaryota
ENOG410Y3KP LUCA
GeneTreeiENSGT00510000048252
HOGENOMiHOG000013069
HOVERGENiHBG052567
InParanoidiQ9CQX4
OMAiQPDHSDD
OrthoDBiEOG091G13N6
PhylomeDBiQ9CQX4
TreeFamiTF333199

Enzyme and pathway databases

ReactomeiR-MMU-5656169 Termination of translesion DNA synthesis

Miscellaneous databases

PROiPR:Q9CQX4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000040204 Expressed in 172 organ(s), highest expression level in thymus
CleanExiMM_2810417H13RIK
GenevisibleiQ9CQX4 MM

Family and domain databases

InterProiView protein in InterPro
IPR031444 PCNA-AF
PANTHERiPTHR15679 PTHR15679, 1 hit
PfamiView protein in Pfam
PF15715 PAF, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPAF15_MOUSE
AccessioniPrimary (citable) accession number: Q9CQX4
Secondary accession number(s): Q4VAG2, Q9CZ17, Q9D0A5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 27, 2004
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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