UniProtKB - Q9CQW9 (IFM3_MOUSE)
Protein
Interferon-induced transmembrane protein 3
Gene
Ifitm3
Organism
Mus musculus (Mouse)
Status
Functioni
IFN-induced antiviral protein which inhibits the entry of viruses to the host cell cytoplasm, permitting endocytosis, but preventing subsequent viral fusion and release of viral contents into the cytosol. Active against multiple viruses, including influenza A virus, SARS coronavirus (SARS-CoV), Marburg virus (MARV) and Ebola virus (EBOV), Dengue virus (DNV), West Nile virus (WNV) and human immunodeficiency virus type 1 (HIV-1). Can inhibit: influenza virus hemagglutinin protein-mediated viral entry, MARV and EBOV GP1,2-mediated viral entry and SARS-CoV S protein-mediated viral entry. Plays a critical role in the structural stability and function of vacuolar ATPase (v-ATPase). Establishes physical contact with the v-ATPase of endosomes which is critical for proper clathrin localization and is also required for the function of the v-ATPase to lower the pH in phagocytic endosomes thus establishing an antiviral state. Involved in initiating germ cell competence and specification, and in the demarcation of PGCs from their somatic neighbors.4 Publications
Caution
It has been previously shown that mediates migration of early primordial germ cells (PGCs) (PubMed:16326387). But according to PubMed:16326387, have no detectable effects on development of the germ line or on the generation of live young, hence, is not essential for PGC migration.1 Publication
GO - Biological processi
- defense response to virus Source: MGI
- negative regulation of cell population proliferation Source: MGI
- negative regulation of viral entry into host cell Source: UniProtKB
- negative regulation of viral genome replication Source: MGI
- negative regulation of viral transcription Source: MGI
- receptor-mediated endocytosis Source: MGI
- response to interferon-alpha Source: MGI
- response to interferon-beta Source: MGI
- response to interferon-gamma Source: MGI
- response to virus Source: UniProtKB
- type I interferon signaling pathway Source: MGI
Keywordsi
Biological process | Antiviral defense, Immunity, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Names & Taxonomyi
Protein namesi | Recommended name: Interferon-induced transmembrane protein 3Alternative name(s): Dispanin subfamily A member 2b Short name: DSPA2b Fragilis protein Interferon-inducible protein 15 Mouse ifitm-like protein 1 Short name: Mil-1 |
Gene namesi | Name:Ifitm3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913391 Ifitm3 |
Subcellular locationi
Endosome
- Late endosome membrane By similarity; Single-pass type II membrane protein By similarity
Plasma membrane
Lysosome
- Lysosome membrane By similarity; Single-pass type II membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
Endosome
- late endosome membrane Source: UniProtKB-SubCell
Lysosome
- lysosomal membrane Source: UniProtKB-SubCell
Nucleus
- nucleus Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- apical part of cell Source: MGI
- cell surface Source: MGI
- cytoplasm Source: MGI
- cytoplasmic vesicle Source: MGI
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 57 | CytoplasmicSequence analysisAdd BLAST | 57 | |
Intramembranei | 58 – 78 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 79 – 109 | CytoplasmicSequence analysisAdd BLAST | 31 | |
Transmembranei | 110 – 130 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 131 – 137 | ExtracellularSequence analysis | 7 |
Keywords - Cellular componenti
Cell membrane, Endosome, Lysosome, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000398567 | 1 – 137 | Interferon-induced transmembrane protein 3Add BLAST | 137 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Cross-linki | 24 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Modified residuei | 27 | PhosphotyrosineCombined sources | 1 | |
Lipidationi | 71 | S-palmitoyl cysteineBy similarity | 1 | |
Lipidationi | 72 | S-palmitoyl cysteineBy similarity | 1 | |
Cross-linki | 83 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 88 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Cross-linki | 104 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity | ||
Lipidationi | 105 | S-palmitoyl cysteineBy similarity | 1 |
Post-translational modificationi
Polyubiquitinated with both 'Lys-48' and 'Lys-63' linkages. Ubiquitination negatively regulates antiviral activity. Lys-24 is the most prevalent ubiquitination site (By similarity).By similarity
Keywords - PTMi
Isopeptide bond, Lipoprotein, Palmitate, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9CQW9 |
jPOSTi | Q9CQW9 |
MaxQBi | Q9CQW9 |
PaxDbi | Q9CQW9 |
PeptideAtlasi | Q9CQW9 |
PRIDEi | Q9CQW9 |
PTM databases
iPTMneti | Q9CQW9 |
PhosphoSitePlusi | Q9CQW9 |
SwissPalmi | Q9CQW9 |
Expressioni
Tissue specificityi
Expressed in acinar cell. Predominantly expressed in nascent primordial germ cells, as well as in gonadal germ cells.2 Publications
Developmental stagei
At 7.25 dpc strong expression is found at the base of the incipient allantois and weak expression in the mesodermal portion of the posterior amnion, and, importantly, the expression did not extend to the allantois. Expression persisted until the late bud stage (7.5 dpc), but gradually faded around the early head fold stage (7.75 dpc). At an earlier stage, only weak expression is seen throughout the epiblast in 6.0 dpc. But around 6.25-6.5 dpc (before gastrulation), marked expression is evident within the most proximal layer of the epiblast that is in intimate contact with the extraembryonic ectoderm. Expression is indeed induced by extraembryonic ectoderm through signaling molecules. During germ cell formation, is expressed in putative PGC ancestors in embryos at 6.5-7.5 dpc. In migrating PGCs, expression is continuous. After the beginning of gastrulation, the expression migrates to the posterior end of the developing primitive streak at the early/mid streak stage and became very intense in the position where PGCs (Primordial germ cells) differentiate from late streak stage onward.3 Publications
Inductioni
By alpha interferon. Induced in pancreas during caerulein-induced pancreatitis. Induced in pancreas under systemic-lipopolysaccharide treatment and in intestine under Salmonella infection.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000025492 Expressed in 294 organ(s), highest expression level in uterine cervix |
Genevisiblei | Q9CQW9 MM |
Interactioni
Subunit structurei
Interacts with SPP1; the interaction reduces OPN expression (By similarity). Interacts with ATP6V0B and CD81.By similarity2 Publications
Protein-protein interaction databases
BioGridi | 211244, 5 interactors |
IntActi | Q9CQW9, 3 interactors |
MINTi | Q9CQW9 |
STRINGi | 10090.ENSMUSP00000026565 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 60 – 93 | Interaction with SPP1By similarityAdd BLAST | 34 |
Sequence similaritiesi
Belongs to the CD225/Dispanin family.Curated
Keywords - Domaini
Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG410J2DU Eukaryota ENOG4112C1U LUCA |
GeneTreei | ENSGT00390000003476 |
HOGENOMi | HOG000115781 |
HOVERGENi | HBG001182 |
InParanoidi | Q9CQW9 |
KOi | K06566 |
OMAi | YGRTAKI |
OrthoDBi | 1555189at2759 |
PhylomeDBi | Q9CQW9 |
TreeFami | TF334894 |
Family and domain databases
InterProi | View protein in InterPro IPR007593 CD225/Dispanin_fam |
Pfami | View protein in Pfam PF04505 CD225, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9CQW9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNHTSQAFIT AASGGQPPNY ERIKEEYEVA EMGAPHGSAS VRTTVINMPR
60 70 80 90 100
EVSVPDHVVW SLFNTLFMNF CCLGFIAYAY SVKSRDRKMV GDVTGAQAYA
110 120 130
STAKCLNIST LVLSILMVVI TIVSVIIIVL NAQNLHT
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 130 | L → F in BAB25347 (PubMed:15184081).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY082484 mRNA Translation: AAM03316.1 AY594690 mRNA Translation: AAT06089.1 AK003407 mRNA Translation: BAB22771.1 AK007916 mRNA Translation: BAB25347.1 AK007919 mRNA Translation: BAB25350.1 AK151890 mRNA Translation: BAE30774.1 CH466531 Genomic DNA Translation: EDL17977.1 BC010291 mRNA Translation: AAH10291.1 |
CCDSi | CCDS21996.1 |
RefSeqi | NP_079654.1, NM_025378.2 |
UniGenei | Mm.141021 |
Genome annotation databases
Ensembli | ENSMUST00000026565; ENSMUSP00000026565; ENSMUSG00000025492 |
GeneIDi | 66141 |
KEGGi | mmu:66141 |
UCSCi | uc009kjc.1 mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AY082484 mRNA Translation: AAM03316.1 AY594690 mRNA Translation: AAT06089.1 AK003407 mRNA Translation: BAB22771.1 AK007916 mRNA Translation: BAB25347.1 AK007919 mRNA Translation: BAB25350.1 AK151890 mRNA Translation: BAE30774.1 CH466531 Genomic DNA Translation: EDL17977.1 BC010291 mRNA Translation: AAH10291.1 |
CCDSi | CCDS21996.1 |
RefSeqi | NP_079654.1, NM_025378.2 |
UniGenei | Mm.141021 |
3D structure databases
ModBasei | Search... |
MobiDBi | Search... |
Protein-protein interaction databases
BioGridi | 211244, 5 interactors |
IntActi | Q9CQW9, 3 interactors |
MINTi | Q9CQW9 |
STRINGi | 10090.ENSMUSP00000026565 |
PTM databases
iPTMneti | Q9CQW9 |
PhosphoSitePlusi | Q9CQW9 |
SwissPalmi | Q9CQW9 |
Proteomic databases
EPDi | Q9CQW9 |
jPOSTi | Q9CQW9 |
MaxQBi | Q9CQW9 |
PaxDbi | Q9CQW9 |
PeptideAtlasi | Q9CQW9 |
PRIDEi | Q9CQW9 |
Protocols and materials databases
Structural Biology Knowledgebase | Search... |
Genome annotation databases
Ensembli | ENSMUST00000026565; ENSMUSP00000026565; ENSMUSG00000025492 |
GeneIDi | 66141 |
KEGGi | mmu:66141 |
UCSCi | uc009kjc.1 mouse |
Organism-specific databases
CTDi | 10410 |
MGIi | MGI:1913391 Ifitm3 |
Phylogenomic databases
eggNOGi | ENOG410J2DU Eukaryota ENOG4112C1U LUCA |
GeneTreei | ENSGT00390000003476 |
HOGENOMi | HOG000115781 |
HOVERGENi | HBG001182 |
InParanoidi | Q9CQW9 |
KOi | K06566 |
OMAi | YGRTAKI |
OrthoDBi | 1555189at2759 |
PhylomeDBi | Q9CQW9 |
TreeFami | TF334894 |
Enzyme and pathway databases
Reactomei | R-MMU-198933 Immunoregulatory interactions between a Lymphoid and a non-Lymphoid cell |
Miscellaneous databases
ChiTaRSi | Ifitm3 mouse |
PROi | PR:Q9CQW9 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025492 Expressed in 294 organ(s), highest expression level in uterine cervix |
Genevisiblei | Q9CQW9 MM |
Family and domain databases
InterProi | View protein in InterPro IPR007593 CD225/Dispanin_fam |
Pfami | View protein in Pfam PF04505 CD225, 1 hit |
ProtoNeti | Search... |
Entry informationi
Entry namei | IFM3_MOUSE | |
Accessioni | Q9CQW9Primary (citable) accession number: Q9CQW9 Secondary accession number(s): Q9D8L6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 13, 2019 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot