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Protein

14-3-3 protein beta/alpha

Gene

Ywhab

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Adapter protein implicated in the regulation of a large spectrum of both general and specialized signaling pathways. Binds to a large number of partners, usually by recognition of a phosphoserine or phosphothreonine motif. Binding generally results in the modulation of the activity of the binding partner. Negative regulator of osteogenesis. Blocks the nuclear translocation of the phosphorylated form (by AKT1) of SRPK2 and antagonizes its stimulatory effect on cyclin D1 expression resulting in blockage of neuronal apoptosis elicited by SRPK2. Negative regulator of signaling cascades that mediate activation of MAP kinases via AKAP13.By similarity

GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

ReactomeiR-MMU-111447 Activation of BAD and translocation to mitochondria
R-MMU-1445148 Translocation of GLUT4 to the plasma membrane
R-MMU-165159 mTOR signalling
R-MMU-166208 mTORC1-mediated signalling
R-MMU-2028269 Signaling by Hippo
R-MMU-392517 Rap1 signalling
R-MMU-450385 Butyrate Response Factor 1 (BRF1) binds and destabilizes mRNA
R-MMU-5625740 RHO GTPases activate PKNs
R-MMU-5628897 TP53 Regulates Metabolic Genes
R-MMU-5673000 RAF activation
R-MMU-5674135 MAP2K and MAPK activation
R-MMU-5675221 Negative regulation of MAPK pathway
R-MMU-75035 Chk1/Chk2(Cds1) mediated inactivation of Cyclin B:Cdk1 complex

Names & Taxonomyi

Protein namesi
Recommended name:
14-3-3 protein beta/alpha
Alternative name(s):
Protein kinase C inhibitor protein 1
Short name:
KCIP-1
Cleaved into the following chain:
Gene namesi
Name:Ywhab
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 2

Organism-specific databases

MGIiMGI:1891917 Ywhab

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003679021 – 24614-3-3 protein beta/alphaAdd BLAST246
Initiator methionineiRemoved; alternateBy similarity
ChainiPRO_00000000052 – 24614-3-3 protein beta/alpha, N-terminally processedAdd BLAST245

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei2N-acetylthreonine; in 14-3-3 protein beta/alpha, N-terminally processedBy similarity1
Modified residuei2PhosphothreonineBy similarity1
Modified residuei5N6-acetyllysineBy similarity1
Modified residuei51N6-acetyllysine; alternateBy similarity1
Cross-linki51Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateBy similarity
Modified residuei60Phosphoserine1 Publication1
Modified residuei70N6-acetyllysineBy similarity1
Modified residuei84Nitrated tyrosineCombined sources1
Modified residuei106Nitrated tyrosineCombined sources1
Modified residuei117N6-acetyllysineBy similarity1
Modified residuei186PhosphoserineBy similarity1
Modified residuei232PhosphoserineBy similarity1
Isoform Short (identifier: Q9CQV8-2)
Modified residuei1N-acetylmethionineBy similarity1

Post-translational modificationi

Isoform alpha differs from isoform beta in being phosphorylated (By similarity). Phosphorylated on Ser-60 by protein kinase C delta type catalytic subunit in a sphingosine-dependent fashion.By similarity1 Publication
Isoform Short contains a N-acetylmethionine at position 1.By similarity

Keywords - PTMi

Acetylation, Isopeptide bond, Nitration, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiQ9CQV8
PeptideAtlasiQ9CQV8
PRIDEiQ9CQV8
TopDownProteomicsiQ9CQV8-1 [Q9CQV8-1]

2D gel databases

UCD-2DPAGEiQ9CQV8

PTM databases

iPTMnetiQ9CQV8
PhosphoSitePlusiQ9CQV8
SwissPalmiQ9CQV8

Expressioni

Gene expression databases

BgeeiENSMUSG00000018326
ExpressionAtlasiQ9CQV8 baseline and differential
GenevisibleiQ9CQV8 MM

Interactioni

Subunit structurei

Homodimer (By similarity). Interacts with SAMSN1 and PRKCE (PubMed:18604201, PubMed:20478393). Interacts with AKAP13. Interacts with SSH1 and TORC2/CRTC2. Interacts with ABL1; the interaction results in cytoplasmic location of ABL1 and inhibition of cABL-mediated apoptosis. Interacts with ROR2 (dimer); the interaction results in phosphorylation of YWHAB on tyrosine residues. Interacts with GAB2. Interacts with YAP1 (phosphorylated form). Interacts with the phosphorylated (by AKT1) form of SRPK2. Interacts with PKA-phosphorylated AANAT. Interacts with MYO1C. Interacts with SIRT2 (By similarity). Interacts with the 'Thr-369' phosphorylated form of DAPK2 (PubMed:26047703). Interacts with PI4KB, TBC1D22A and TBC1D22B. Interacts with the 'Ser-1134' and 'Ser-1161' phosphorylated form of SOS1 (By similarity). Interacts (via phosphorylated form) with YWHAB; this interaction occurs in a protein kinase AKT1-dependent manner (By similarity). Interacts with SLITRK1 (By similarity). Interacts with SYNPO2 (phosphorylated form); YWHAB competes with ACTN2 for interaction with SYNPO2 (PubMed:15883195). Interacts with RIPOR2 (via phosphorylated form); this interaction occurs in a chemokine-dependent manner and does not compete for binding of RIPOR2 with RHOA nor blocks inhibition of RIPOR2-mediated RHOA activity (By similarity).By similarity4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei58Interaction with phosphoserine on interacting proteinBy similarity1
Sitei129Interaction with phosphoserine on interacting proteinBy similarity1

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi207648, 31 interactors
IntActiQ9CQV8, 629 interactors
MINTiQ9CQV8
STRINGi10090.ENSMUSP00000018470

Structurei

Secondary structure

1246
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi5 – 17Combined sources13
Helixi21 – 32Combined sources12
Helixi40 – 68Combined sources29
Helixi77 – 105Combined sources29
Helixi107 – 110Combined sources4
Helixi114 – 134Combined sources21
Helixi137 – 161Combined sources25
Helixi167 – 182Combined sources16
Helixi187 – 203Combined sources17
Helixi205 – 207Combined sources3
Turni210 – 212Combined sources3
Helixi213 – 230Combined sources18

3D structure databases

ProteinModelPortaliQ9CQV8
SMRiQ9CQV8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the 14-3-3 family.Curated

Phylogenomic databases

eggNOGiKOG0841 Eukaryota
COG5040 LUCA
GeneTreeiENSGT00760000119116
HOGENOMiHOG000240379
HOVERGENiHBG050423
InParanoidiQ9CQV8
KOiK16197
OMAiKKQQMGR
OrthoDBiEOG091G0VKY
PhylomeDBiQ9CQV8
TreeFamiTF102003

Family and domain databases

Gene3Di1.20.190.20, 1 hit
InterProiView protein in InterPro
IPR000308 14-3-3
IPR023409 14-3-3_CS
IPR036815 14-3-3_dom_sf
IPR023410 14-3-3_domain
PANTHERiPTHR18860 PTHR18860, 1 hit
PfamiView protein in Pfam
PF00244 14-3-3, 1 hit
PIRSFiPIRSF000868 14-3-3, 1 hit
PRINTSiPR00305 1433ZETA
SMARTiView protein in SMART
SM00101 14_3_3, 1 hit
SUPFAMiSSF48445 SSF48445, 1 hit
PROSITEiView protein in PROSITE
PS00796 1433_1, 1 hit
PS00797 1433_2, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform Long (identifier: Q9CQV8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTMDKSELVQ KAKLAEQAER YDDMAAAMKA VTEQGHELSN EERNLLSVAY
60 70 80 90 100
KNVVGARRSS WRVISSIEQK TERNEKKQQM GKEYREKIEA ELQDICNDVL
110 120 130 140 150
ELLDKYLILN ATQAESKVFY LKMKGDYFRY LSEVASGENK QTTVSNSQQA
160 170 180 190 200
YQEAFEISKK EMQPTHPIRL GLALNFSVFY YEILNSPEKA CSLAKTAFDE
210 220 230 240
AIAELDTLNE ESYKDSTLIM QLLRDNLTLW TSENQGDEGD AGEGEN
Length:246
Mass (Da):28,086
Last modified:January 23, 2007 - v3
Checksum:i51C366ED85B38EED
GO
Isoform Short (identifier: Q9CQV8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-2: Missing.

Note: No experimental confirmation available.By similarity
Show »
Length:244
Mass (Da):27,854
Checksum:iF42896ED9EC6F0D5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti10Q → H in AAC14343 (Ref. 1) Curated1
Sequence conflicti74N → D in AAC14343 (Ref. 1) Curated1
Sequence conflicti126D → Y in BAE29538 (PubMed:16141072).Curated1
Sequence conflicti126D → Y in BAE30278 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0186341 – 2Missing in isoform Short. Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF058797 mRNA Translation: AAC14343.1
AK002632 mRNA Translation: BAB22246.1
AK004872 mRNA Translation: BAB23631.1
AK011389 mRNA Translation: BAB27587.1
AK083367 mRNA Translation: BAC38886.1
AK144061 mRNA Translation: BAE25678.1
AK150414 mRNA Translation: BAE29538.1
AK151294 mRNA Translation: BAE30278.1
AK158932 mRNA Translation: BAE34730.1
CCDSiCCDS17019.1 [Q9CQV8-1]
RefSeqiNP_061223.2, NM_018753.6 [Q9CQV8-1]
XP_006499972.1, XM_006499909.1 [Q9CQV8-1]
UniGeneiMm.34319
Mm.491117

Genome annotation databases

EnsembliENSMUST00000018470; ENSMUSP00000018470; ENSMUSG00000018326 [Q9CQV8-1]
GeneIDi54401
KEGGimmu:54401
UCSCiuc008ntp.1 mouse [Q9CQV8-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Entry informationi

Entry namei1433B_MOUSE
AccessioniPrimary (citable) accession number: Q9CQV8
Secondary accession number(s): O70455, Q3TY33, Q3UAN6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2001
Last sequence update: January 23, 2007
Last modified: July 18, 2018
This is version 169 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

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