UniProtKB - Q9CQV1 (TIM16_MOUSE)
Mitochondrial import inner membrane translocase subunit TIM16
Pam16
Functioni
Regulates ATP-dependent protein translocation into the mitochondrial matrix. Inhibits DNAJC19 stimulation of HSPA9/Mortalin ATPase activity.
By similarityGO - Biological processi
- DNA biosynthetic process Source: MGI
- negative regulation of apoptotic DNA fragmentation Source: MGI
- negative regulation of apoptotic process Source: MGI
- negative regulation of apoptotic signaling pathway Source: MGI
- negative regulation of ATPase activity Source: MGI
- negative regulation of release of cytochrome c from mitochondria Source: MGI
- ossification Source: UniProtKB
- protein import into mitochondrial matrix Source: MGI
Keywordsi
Biological process | Protein transport, Translocation, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Mitochondrial import inner membrane translocase subunit TIM16CuratedAlternative name(s): Mitochondria-associated granulocyte macrophage CSF-signaling molecule Presequence translocated-associated motor subunit PAM16 |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1913699, Pam16 |
VEuPathDBi | HostDB:ENSMUSG00000014301 HostDB:ENSMUSG00000045886 |
Subcellular locationi
Mitochondrion
- Mitochondrion inner membrane By similarity; Peripheral membrane protein By similarity; Matrix side By similarity
Mitochondrion
- extrinsic component of mitochondrial inner membrane Source: MGI
- mitochondrial inner membrane Source: MGI
- mitochondrial matrix Source: MGI
- mitochondrion Source: MGI
- PAM complex, Tim23 associated import motor Source: UniProtKB
- TIM23 mitochondrial import inner membrane translocase complex Source: GO_Central
Other locations
- cytoplasm Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Membrane, Mitochondrion, Mitochondrion inner membranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000214079 | 1 – 125 | Mitochondrial import inner membrane translocase subunit TIM16Add BLAST | 125 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 69 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9CQV1 |
jPOSTi | Q9CQV1 |
MaxQBi | Q9CQV1 |
PaxDbi | Q9CQV1 |
PeptideAtlasi | Q9CQV1 |
PRIDEi | Q9CQV1 |
ProteomicsDBi | 262784 |
TopDownProteomicsi | Q9CQV1 |
PTM databases
iPTMneti | Q9CQV1 |
PhosphoSitePlusi | Q9CQV1 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000014301, Expressed in muscle tissue and 72 other tissues |
ExpressionAtlasi | Q9CQV1, baseline and differential |
Genevisiblei | Q9CQV1, MM |
Interactioni
Subunit structurei
Probable component of the PAM complex at least composed of a mitochondrial HSP70 protein, GRPEL1 or GRPEL2, TIMM44, TIMM16/PAM16 and TIMM14/DNAJC19 (By similarity).
Interacts with DNAJC19. Directly interacts with DNAJC15; this interaction counteracts DNAJC15-dependent stimulation of HSPA9 ATPase activity (By similarity). Associates with the TIM23 complex (Probable).
By similarity1 PublicationProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000014445 |
Miscellaneous databases
RNActi | Q9CQV1, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 58 – 110 | J-likeAdd BLAST | 53 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3442, Eukaryota |
GeneTreei | ENSGT00390000012037 |
HOGENOMi | CLU_101461_3_0_1 |
InParanoidi | Q9CQV1 |
OMAi | AKYLIQI |
OrthoDBi | 1640138at2759 |
PhylomeDBi | Q9CQV1 |
TreeFami | TF315134 |
Family and domain databases
Gene3Di | 1.10.287.110, 1 hit |
InterProi | View protein in InterPro IPR036869, J_dom_sf IPR005341, Tim16 |
PANTHERi | PTHR12388, PTHR12388, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAKYLAQIIV MGVQVVGRAF ARALRQEFAA SQAAADARGR AGHQSAAASN
60 70 80 90 100
LSGLSLQEAQ QILNVSKLSP EEVQKNYEHL FKVNDKSVGG SFYLQSKVVR
110 120
AKERLDEELR IQAQEDREKG QKPKT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SQA2 | A0A1L1SQA2_MOUSE | Presequence translocase-associated ... | Pam16l | 97 | Annotation score: | ||
D6RI93 | D6RI93_MOUSE | Mitochondrial import inner membrane... | Pam16 | 39 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF349454 mRNA Translation: AAL57766.1 AY320045 Genomic DNA Translation: AAQ86806.1 AB073618 mRNA Translation: BAB91135.1 AK003227 mRNA Translation: BAB22656.1 AK008380 mRNA Translation: BAB25635.1 BC024346 mRNA Translation: AAH24346.1 BC096419 mRNA Translation: AAH96419.1 |
CCDSi | CCDS37241.1 |
RefSeqi | NP_079847.1, NM_025571.1 |
Genome annotation databases
Ensembli | ENSMUST00000014445; ENSMUSP00000014445; ENSMUSG00000014301 ENSMUST00000057649; ENSMUSP00000137140; ENSMUSG00000045886 |
GeneIDi | 66449 |
KEGGi | mmu:66449 |
UCSCi | uc007xzx.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF349454 mRNA Translation: AAL57766.1 AY320045 Genomic DNA Translation: AAQ86806.1 AB073618 mRNA Translation: BAB91135.1 AK003227 mRNA Translation: BAB22656.1 AK008380 mRNA Translation: BAB25635.1 BC024346 mRNA Translation: AAH24346.1 BC096419 mRNA Translation: AAH96419.1 |
CCDSi | CCDS37241.1 |
RefSeqi | NP_079847.1, NM_025571.1 |
3D structure databases
SMRi | Q9CQV1 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000014445 |
PTM databases
iPTMneti | Q9CQV1 |
PhosphoSitePlusi | Q9CQV1 |
Proteomic databases
EPDi | Q9CQV1 |
jPOSTi | Q9CQV1 |
MaxQBi | Q9CQV1 |
PaxDbi | Q9CQV1 |
PeptideAtlasi | Q9CQV1 |
PRIDEi | Q9CQV1 |
ProteomicsDBi | 262784 |
TopDownProteomicsi | Q9CQV1 |
Protocols and materials databases
DNASUi | 66449 |
Genome annotation databases
Ensembli | ENSMUST00000014445; ENSMUSP00000014445; ENSMUSG00000014301 ENSMUST00000057649; ENSMUSP00000137140; ENSMUSG00000045886 |
GeneIDi | 66449 |
KEGGi | mmu:66449 |
UCSCi | uc007xzx.1, mouse |
Organism-specific databases
CTDi | 51025 |
MGIi | MGI:1913699, Pam16 |
VEuPathDBi | HostDB:ENSMUSG00000014301 HostDB:ENSMUSG00000045886 |
Phylogenomic databases
eggNOGi | KOG3442, Eukaryota |
GeneTreei | ENSGT00390000012037 |
HOGENOMi | CLU_101461_3_0_1 |
InParanoidi | Q9CQV1 |
OMAi | AKYLIQI |
OrthoDBi | 1640138at2759 |
PhylomeDBi | Q9CQV1 |
TreeFami | TF315134 |
Miscellaneous databases
BioGRID-ORCSi | 66449, 24 hits in 61 CRISPR screens |
PROi | PR:Q9CQV1 |
RNActi | Q9CQV1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000014301, Expressed in muscle tissue and 72 other tissues |
ExpressionAtlasi | Q9CQV1, baseline and differential |
Genevisiblei | Q9CQV1, MM |
Family and domain databases
Gene3Di | 1.10.287.110, 1 hit |
InterProi | View protein in InterPro IPR036869, J_dom_sf IPR005341, Tim16 |
PANTHERi | PTHR12388, PTHR12388, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | TIM16_MOUSE | |
Accessioni | Q9CQV1Primary (citable) accession number: Q9CQV1 Secondary accession number(s): Q6EIX1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families