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Protein

Acyl-coenzyme A thioesterase 13

Gene

Acot13

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Acyl-CoA thioesterases are a group of enzymes that catalyze the hydrolysis of acyl-CoAs to the free fatty acid and coenzyme A (CoASH), providing the potential to regulate intracellular levels of acyl-CoAs, free fatty acids and CoASH. Has acyl-CoA thioesterase activity towards medium (C12) and long-chain (C18) fatty acyl-CoA substrates. Can also hydrolyze 3-hydroxyphenylacetyl-CoA (in vitro). May play a role in controlling adaptive thermogenesis.2 Publications

Kineticsi

  1. KM=47 µM for decanoyl-CoA1 Publication
  2. KM=27 µM for oleoyl-CoA1 Publication
  3. KM=10 µM for palmitoyl-CoA1 Publication
  4. KM=15 µM for myristoyl-CoA1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei50SubstrateBy similarity1

    GO - Molecular functioni

    GO - Biological processi

    • negative regulation of cold-induced thermogenesis Source: YuBioLab
    • protein homotetramerization Source: MGI

    Keywordsi

    Molecular functionHydrolase

    Enzyme and pathway databases

    ReactomeiR-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
    SABIO-RKiQ9CQR4

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Acyl-coenzyme A thioesterase 13 (EC:3.1.2.-)
    Short name:
    Acyl-CoA thioesterase 13
    Alternative name(s):
    Thioesterase superfamily member 2
    Gene namesi
    Name:Acot13
    Synonyms:Them2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 13

    Organism-specific databases

    MGIiMGI:1914084 Acot13

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Keywords - Cellular componenti

    Cytoplasm, Cytoskeleton, Mitochondrion, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    No visible phenotype until 7 weeks of age. Animals are viable and fertile. After 7 weeks, mutant mice exhibit a modest decrease in body weight and decreased adiposity, compared to wild-type animals, despite increased food consumption. They tend to show a reduced hepatic fatty acyl-CoA thioesterase activity, leading to alterations in fatty acid metabolism and improved glucose homeostasis. When fed a high-fat diet, mutant livers are protected against steatosis and increased hepatic glucose production (PubMed:22345407). Mutant mice adapt more rapidly than wild-type to short-term cold exposure by increasing physical activity, food consumption and energy expenditure. After 96-hour equilibration at cold temperature, genotype-dependent differences are abolished. Mutant brown adipose tissue show reduced lipid droplets, alterations in the ultrastructure of mitochondria and a small increase in the expression of thermogenic genes (PubMed:24072708).2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001566981 – 140Acyl-coenzyme A thioesterase 13Add BLAST140

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Modified residuei1N-acetylmethionineBy similarity1
    Modified residuei27N6-acetyllysineCombined sources1
    Modified residuei37N6-acetyllysineCombined sources1
    Modified residuei43N6-acetyllysineCombined sources1
    Modified residuei108N6-acetyllysineCombined sources1
    Modified residuei127N6-acetyllysineCombined sources1

    Keywords - PTMi

    Acetylation

    Proteomic databases

    EPDiQ9CQR4
    MaxQBiQ9CQR4
    PaxDbiQ9CQR4
    PRIDEiQ9CQR4

    2D gel databases

    REPRODUCTION-2DPAGEiQ9CQR4

    PTM databases

    iPTMnetiQ9CQR4
    PhosphoSitePlusiQ9CQR4

    Expressioni

    Tissue specificityi

    Highly expressed in the kidney and moderately in the heart, liver, brain, small and large intestine. Also expressed in brown adipose tissue.4 Publications

    Gene expression databases

    BgeeiENSMUSG00000006717 Expressed in 291 organ(s), highest expression level in heart
    CleanExiMM_THEM2
    ExpressionAtlasiQ9CQR4 baseline and differential
    GenevisibleiQ9CQR4 MM

    Interactioni

    Subunit structurei

    Homotetramer. Interacts with PCTP.2 Publications

    Protein-protein interaction databases

    BioGridi211751, 1 interactor
    IntActiQ9CQR4, 2 interactors
    MINTiQ9CQR4
    STRINGi10090.ENSMUSP00000006900

    Structurei

    Secondary structure

    1140
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    ProteinModelPortaliQ9CQR4
    SMRiQ9CQR4
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9CQR4

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the thioesterase PaaI family.Curated

    Phylogenomic databases

    eggNOGiKOG3328 Eukaryota
    COG2050 LUCA
    GeneTreeiENSGT00390000013934
    HOGENOMiHOG000170540
    InParanoidiQ9CQR4
    KOiK17362
    OMAiKMGTLHG
    OrthoDBiEOG091G125K
    PhylomeDBiQ9CQR4
    TreeFamiTF315062

    Family and domain databases

    InterProiView protein in InterPro
    IPR039298 ACOT13
    IPR029069 HotDog_dom_sf
    IPR003736 PAAI_dom
    IPR006683 Thioestr_dom
    PANTHERiPTHR21660 PTHR21660, 1 hit
    PfamiView protein in Pfam
    PF03061 4HBT, 1 hit
    SUPFAMiSSF54637 SSF54637, 1 hit
    TIGRFAMsiTIGR00369 unchar_dom_1, 1 hit

    Sequencei

    Sequence statusi: Complete.

    Q9CQR4-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MSSMTQNLRE VMKVMFKVPG FDRVLEKVTL VSAAPEKLIC EMKVEEQHTN
    60 70 80 90 100
    KLGTLHGGLT ATLVDSISTM ALMCTERGAP GVSVDMNITY MSPAKIGEEI
    110 120 130 140
    VITAHILKQG KTLAFASVDL TNKTTGKLIA QGRHTKHLGN
    Length:140
    Mass (Da):15,183
    Last modified:June 1, 2001 - v1
    Checksum:i66E47830E8643051
    GO

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK002261 mRNA Translation: BAB21973.1
    AK008624 mRNA Translation: BAB25786.1
    BC018165 mRNA Translation: AAH18165.1
    CCDSiCCDS26380.1
    RefSeqiNP_080066.1, NM_025790.2
    UniGeneiMm.2125

    Genome annotation databases

    EnsembliENSMUST00000006900; ENSMUSP00000006900; ENSMUSG00000006717
    GeneIDi66834
    KEGGimmu:66834
    UCSCiuc007pwj.1 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AK002261 mRNA Translation: BAB21973.1
    AK008624 mRNA Translation: BAB25786.1
    BC018165 mRNA Translation: AAH18165.1
    CCDSiCCDS26380.1
    RefSeqiNP_080066.1, NM_025790.2
    UniGeneiMm.2125

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    2CY9X-ray2.72A/B1-140[»]
    ProteinModelPortaliQ9CQR4
    SMRiQ9CQR4
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    BioGridi211751, 1 interactor
    IntActiQ9CQR4, 2 interactors
    MINTiQ9CQR4
    STRINGi10090.ENSMUSP00000006900

    PTM databases

    iPTMnetiQ9CQR4
    PhosphoSitePlusiQ9CQR4

    2D gel databases

    REPRODUCTION-2DPAGEiQ9CQR4

    Proteomic databases

    EPDiQ9CQR4
    MaxQBiQ9CQR4
    PaxDbiQ9CQR4
    PRIDEiQ9CQR4

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000006900; ENSMUSP00000006900; ENSMUSG00000006717
    GeneIDi66834
    KEGGimmu:66834
    UCSCiuc007pwj.1 mouse

    Organism-specific databases

    CTDi55856
    MGIiMGI:1914084 Acot13

    Phylogenomic databases

    eggNOGiKOG3328 Eukaryota
    COG2050 LUCA
    GeneTreeiENSGT00390000013934
    HOGENOMiHOG000170540
    InParanoidiQ9CQR4
    KOiK17362
    OMAiKMGTLHG
    OrthoDBiEOG091G125K
    PhylomeDBiQ9CQR4
    TreeFamiTF315062

    Enzyme and pathway databases

    ReactomeiR-MMU-77289 Mitochondrial Fatty Acid Beta-Oxidation
    SABIO-RKiQ9CQR4

    Miscellaneous databases

    EvolutionaryTraceiQ9CQR4
    PROiPR:Q9CQR4
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000006717 Expressed in 291 organ(s), highest expression level in heart
    CleanExiMM_THEM2
    ExpressionAtlasiQ9CQR4 baseline and differential
    GenevisibleiQ9CQR4 MM

    Family and domain databases

    InterProiView protein in InterPro
    IPR039298 ACOT13
    IPR029069 HotDog_dom_sf
    IPR003736 PAAI_dom
    IPR006683 Thioestr_dom
    PANTHERiPTHR21660 PTHR21660, 1 hit
    PfamiView protein in Pfam
    PF03061 4HBT, 1 hit
    SUPFAMiSSF54637 SSF54637, 1 hit
    TIGRFAMsiTIGR00369 unchar_dom_1, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiACO13_MOUSE
    AccessioniPrimary (citable) accession number: Q9CQR4
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
    Last sequence update: June 1, 2001
    Last modified: September 12, 2018
    This is version 129 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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