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Protein

Glutaredoxin-3

Gene

Glrx3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Together with BOLA2, acts as a cytosolic iron-sulfur (Fe-S) cluster assembly factor that facilitates [2Fe-2S] cluster insertion into a subset of cytosolic proteins (By similarity). Acts as a critical negative regulator of cardiac hypertrophy and a positive inotropic regulator (PubMed:16809552, PubMed:18258855, PubMed:18929570). Required for hemoglobin maturation. Does not possess any thyoredoxin activity since it lacks the conserved motif that is essential for catalytic activity (By similarity).By similarity3 Publications

Miscellaneous

Transgenic mice with cardiac-specific Glrx3 overexpression show that it is a potent inhibitor of cardiac hypertrophy induced by pressure overload (transverse aortic constriction). In addition, overexpression dramatically increases the ventricular function and cardiomyocyte contractility.3 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi161Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1
Metal bindingi263Iron-sulfur (2Fe-2S); shared with dimeric partnerBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

LigandIron, Iron-sulfur, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Glutaredoxin-3
Alternative name(s):
PKC-interacting cousin of thioredoxin
Short name:
PICOT
PKC-theta-interacting protein
Short name:
PKCq-interacting protein
Thioredoxin-like protein 2
Gene namesi
Name:Glrx3
Synonyms:Picot, Txnl2
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1353653 Glrx3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Disruption phenotypei

Homozygous null mutants die in utero sometime between E12.5 and E14.5. E12.5 embryos have a smaller body size and hemorrhage in the head. Heterozygous mutants are fertile and show no abnormalities in growth and development, cardiomyocytes exhibit significantly reduced contractility.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001200202 – 337Glutaredoxin-3Add BLAST336

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei119PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CQM9
MaxQBiQ9CQM9
PaxDbiQ9CQM9
PeptideAtlasiQ9CQM9
PRIDEiQ9CQM9

2D gel databases

REPRODUCTION-2DPAGEiIPI00315550
Q9CQM9

PTM databases

iPTMnetiQ9CQM9
PhosphoSitePlusiQ9CQM9
SwissPalmiQ9CQM9

Expressioni

Gene expression databases

BgeeiENSMUSG00000031068 Expressed in 88 organ(s), highest expression level in spleen
CleanExiMM_GLRX3
ExpressionAtlasiQ9CQM9 baseline and differential
GenevisibleiQ9CQM9 MM

Interactioni

Subunit structurei

Homodimer; the homodimer is independent of 2Fe-2S clusters. Heterotrimer; forms a heterotrimeric complex composed by two BOLA2 molecules and one GLRX3 molecule; linked by [2Fe-2S] clusters. Interacts (via N-terminus) with PRKCQ/PKC-theta (By similarity). Interacts (via C-terminus) with CSRP3 (PubMed:18258855). Interacts with CSRP2 (PubMed:18258855).By similarity1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
Ciapin1Q8WTY44EBI-4319195,EBI-2943068

GO - Molecular functioni

Protein-protein interaction databases

IntActiQ9CQM9, 5 interactors
MINTiQ9CQM9
STRINGi10090.ENSMUSP00000066621

Structurei

Secondary structure

1337
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9CQM9
SMRiQ9CQM9
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9CQM9

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini2 – 119ThioredoxinPROSITE-ProRule annotationAdd BLAST118
Domaini144 – 238Glutaredoxin 1PROSITE-ProRule annotationAdd BLAST95
Domaini239 – 337Glutaredoxin 2PROSITE-ProRule annotationAdd BLAST99

Domaini

The thioredoxin domain lacks the two redox-active cysteines. This strongly suggests that it lacks thioredoxin activity.Curated

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0911 Eukaryota
COG0278 LUCA
GeneTreeiENSGT00550000075030
HOGENOMiHOG000165751
HOVERGENiHBG054719
InParanoidiQ9CQM9
OMAiAKCGFSR
OrthoDBiEOG091G0YN0
PhylomeDBiQ9CQM9
TreeFamiTF314151

Family and domain databases

CDDicd03028 GRX_PICOT_like, 2 hits
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR033658 GRX_PICOT-like
IPR004480 Monothiol_GRX-rel
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10293 PTHR10293, 1 hit
PfamiView protein in Pfam
PF00462 Glutaredoxin, 2 hits
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 3 hits
TIGRFAMsiTIGR00365 TIGR00365, 1 hit
PROSITEiView protein in PROSITE
PS51354 GLUTAREDOXIN_2, 2 hits
PS51352 THIOREDOXIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9CQM9-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAAGAAEAGE AAVAVVEVGS AQQFEELLRL KTKSLLVVHF WAPWAPQCVQ
60 70 80 90 100
MNDVMAELAK EHPHVSFVKL EAEAVPEVSE KYEISSVPTF LFFKNSQKVD
110 120 130 140 150
RLDGAHAPEL TKKVQRHVSS GAFPPSTNEH LKEDLSLRLK KLTHAAPCML
160 170 180 190 200
FMKGTPQEPR CGFSKQMVEI LHKHNIQFSS FDIFSDEEVR QGLKTYSNWP
210 220 230 240 250
TYPQLYVSGE LIGGLDIIKE LEASEELDTI CPKAPKLEER LKVLTNKASV
260 270 280 290 300
MLFMKGNKQE AKCGFSKQIL EILNSTGVEY ETFDILEDEE VRQGLKTFSN
310 320 330
WPTYPQLYVR GDLVGGLDIV KELKDNGELL PILKGEN
Length:337
Mass (Da):37,778
Last modified:June 1, 2001 - v1
Checksum:i74A1C65621B28FA0
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A1B0GS58A0A1B0GS58_MOUSE
Glutaredoxin-3
Glrx3
374Annotation score:
A0A1B0GT04A0A1B0GT04_MOUSE
Glutaredoxin-3
Glrx3
163Annotation score:
A0A1B0GSD3A0A1B0GSD3_MOUSE
Glutaredoxin-3
Glrx3
74Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti76P → T in AAF28842 (PubMed:10636891).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118650 mRNA Translation: AAF28842.1
AK010354 mRNA Translation: BAB26874.1
AK017371 mRNA Translation: BAB30712.1
AK147158 mRNA Translation: BAE27724.1
AK152446 mRNA Translation: BAE31225.1
AK152634 mRNA Translation: BAE31376.1
AK155190 mRNA Translation: BAE33105.1
AK167672 mRNA Translation: BAE39721.1
BC033506 mRNA Translation: AAH33506.1
BC087885 mRNA Translation: AAH87885.1
CCDSiCCDS21948.1
RefSeqiNP_075629.2, NM_023140.5
UniGeneiMm.267692
Mm.388932

Genome annotation databases

EnsembliENSMUST00000064404; ENSMUSP00000066621; ENSMUSG00000031068
GeneIDi30926
KEGGimmu:30926
UCSCiuc009kew.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF118650 mRNA Translation: AAF28842.1
AK010354 mRNA Translation: BAB26874.1
AK017371 mRNA Translation: BAB30712.1
AK147158 mRNA Translation: BAE27724.1
AK152446 mRNA Translation: BAE31225.1
AK152634 mRNA Translation: BAE31376.1
AK155190 mRNA Translation: BAE33105.1
AK167672 mRNA Translation: BAE39721.1
BC033506 mRNA Translation: AAH33506.1
BC087885 mRNA Translation: AAH87885.1
CCDSiCCDS21948.1
RefSeqiNP_075629.2, NM_023140.5
UniGeneiMm.267692
Mm.388932

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1WIKNMR-A241-336[»]
ProteinModelPortaliQ9CQM9
SMRiQ9CQM9
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CQM9, 5 interactors
MINTiQ9CQM9
STRINGi10090.ENSMUSP00000066621

PTM databases

iPTMnetiQ9CQM9
PhosphoSitePlusiQ9CQM9
SwissPalmiQ9CQM9

2D gel databases

REPRODUCTION-2DPAGEiIPI00315550
Q9CQM9

Proteomic databases

EPDiQ9CQM9
MaxQBiQ9CQM9
PaxDbiQ9CQM9
PeptideAtlasiQ9CQM9
PRIDEiQ9CQM9

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000064404; ENSMUSP00000066621; ENSMUSG00000031068
GeneIDi30926
KEGGimmu:30926
UCSCiuc009kew.1 mouse

Organism-specific databases

CTDi10539
MGIiMGI:1353653 Glrx3

Phylogenomic databases

eggNOGiKOG0911 Eukaryota
COG0278 LUCA
GeneTreeiENSGT00550000075030
HOGENOMiHOG000165751
HOVERGENiHBG054719
InParanoidiQ9CQM9
OMAiAKCGFSR
OrthoDBiEOG091G0YN0
PhylomeDBiQ9CQM9
TreeFamiTF314151

Miscellaneous databases

ChiTaRSiGlrx3 mouse
EvolutionaryTraceiQ9CQM9
PROiPR:Q9CQM9
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031068 Expressed in 88 organ(s), highest expression level in spleen
CleanExiMM_GLRX3
ExpressionAtlasiQ9CQM9 baseline and differential
GenevisibleiQ9CQM9 MM

Family and domain databases

CDDicd03028 GRX_PICOT_like, 2 hits
InterProiView protein in InterPro
IPR002109 Glutaredoxin
IPR033658 GRX_PICOT-like
IPR004480 Monothiol_GRX-rel
IPR036249 Thioredoxin-like_sf
IPR013766 Thioredoxin_domain
PANTHERiPTHR10293 PTHR10293, 1 hit
PfamiView protein in Pfam
PF00462 Glutaredoxin, 2 hits
PF00085 Thioredoxin, 1 hit
SUPFAMiSSF52833 SSF52833, 3 hits
TIGRFAMsiTIGR00365 TIGR00365, 1 hit
PROSITEiView protein in PROSITE
PS51354 GLUTAREDOXIN_2, 2 hits
PS51352 THIOREDOXIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiGLRX3_MOUSE
AccessioniPrimary (citable) accession number: Q9CQM9
Secondary accession number(s): Q5I0V8, Q9JLZ2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 26, 2003
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 152 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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