Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Mediator of RNA polymerase II transcription subunit 30

Gene

Med30

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 30
Alternative name(s):
Mediator complex subunit 30
Thyroid hormone receptor-associated protein 6
Thyroid hormone receptor-associated protein complex 25 kDa component
Short name:
Trap25
Gene namesi
Name:Med30
Synonyms:Thrap6, Trap25
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1917040 Med30

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002394072 – 178Mediator of RNA polymerase II transcription subunit 30Add BLAST177

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

EPDiQ9CQI9
MaxQBiQ9CQI9
PaxDbiQ9CQI9
PeptideAtlasiQ9CQI9
PRIDEiQ9CQI9

PTM databases

PhosphoSitePlusiQ9CQI9

Expressioni

Gene expression databases

BgeeiENSMUSG00000038622
CleanExiMM_MED30
GenevisibleiQ9CQI9 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi213682, 2 interactors
ComplexPortaliCPX-3264 Core mediator complex
IntActiQ9CQI9, 14 interactors
MINTiQ9CQI9
STRINGi10090.ENSMUSP00000042204

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili134 – 173Sequence analysisAdd BLAST40

Sequence similaritiesi

Belongs to the Mediator complex subunit 30 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiENOG410IITZ Eukaryota
ENOG4110Q3G LUCA
GeneTreeiENSGT00390000010887
HOGENOMiHOG000006885
HOVERGENiHBG107279
InParanoidiQ9CQI9
KOiK15143
OMAiNCAGLDP
OrthoDBiEOG091G0NPP
PhylomeDBiQ9CQI9
TreeFamiTF324588

Family and domain databases

InterProiView protein in InterPro
IPR021019 Mediator_Med30_met
PANTHERiPTHR31705 PTHR31705, 1 hit
PfamiView protein in Pfam
PF11315 Med30, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9CQI9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTPPLAPTG MASGPFGGPQ AQQAAREVNT ATLCRIGQET VQDIVYRTME
60 70 80 90 100
IFQLLRNMQL PNGVTYHTGT YQDRLTKLQD HLRQLSILFR KLRLVYDKCN
110 120 130 140 150
ENCGGMDPIP VEQLIPYVDE DGSKNDDRAG PPRFASEERR EIVEVNKKLK
160 170
QKNQQLKQIM DQLRNLIWDI NAMLAMRN
Length:178
Mass (Da):20,358
Last modified:June 1, 2001 - v1
Checksum:i8766D3C929C1E98D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK007739 mRNA Translation: BAB25226.1
AK011104 mRNA Translation: BAB27401.1
AK146250 mRNA Translation: BAE27012.1
BC020113 mRNA Translation: AAH20113.1
CCDSiCCDS27465.1
RefSeqiNP_081488.1, NM_027212.2
UniGeneiMm.195562

Genome annotation databases

EnsembliENSMUST00000037115; ENSMUSP00000042204; ENSMUSG00000038622
GeneIDi69790
KEGGimmu:69790
UCSCiuc007vrg.1 mouse

Similar proteinsi

Entry informationi

Entry nameiMED30_MOUSE
AccessioniPrimary (citable) accession number: Q9CQI9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: June 1, 2001
Last modified: July 18, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health