UniProtKB - Q9CQD1 (RAB5A_MOUSE)
Ras-related protein Rab-5A
Rab5a
Functioni
Catalytic activityi
Activity regulationi
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 27 – 35 | GTPBy similarity | 9 | |
Nucleotide bindingi | 46 – 52 | GTPBy similarity | 7 | |
Nucleotide bindingi | 75 – 79 | GTPBy similarity | 5 | |
Nucleotide bindingi | 133 – 136 | GTPBy similarity | 4 | |
Nucleotide bindingi | 163 – 165 | GTPBy similarity | 3 |
GO - Molecular functioni
- GDP binding Source: UniProtKB
- GDP-dissociation inhibitor binding Source: MGI
- GTPase activity Source: MGI
- GTP binding Source: UniProtKB
- guanyl nucleotide binding Source: MGI
GO - Biological processi
- amyloid-beta clearance by transcytosis Source: MGI
- early endosome to late endosome transport Source: MGI
- endocytosis Source: MGI
- endosome organization Source: MGI
- intracellular protein transport Source: GO_Central
- phagocytosis Source: UniProtKB-KW
- positive regulation of exocytosis Source: MGI
- positive regulation of smooth muscle cell migration Source: MGI
- positive regulation of smooth muscle cell proliferation Source: MGI
- receptor internalization involved in canonical Wnt signaling pathway Source: MGI
- regulation of endocytosis Source: GO_Central
- regulation of endosome size Source: MGI
- regulation of filopodium assembly Source: MGI
- regulation of long-term neuronal synaptic plasticity Source: MGI
- regulation of synaptic vesicle exocytosis Source: ParkinsonsUK-UCL
- synaptic vesicle recycling Source: MGI
- viral RNA genome replication Source: MGI
Keywordsi
Molecular function | Hydrolase |
Biological process | Endocytosis, Phagocytosis, Protein transport, Transport |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-1660499, Synthesis of PIPs at the plasma membrane R-MMU-8856828, Clathrin-mediated endocytosis R-MMU-8873719, RAB geranylgeranylation R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Rab5a Synonyms:nnyRab5a |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:105926, Rab5a |
Subcellular locationi
Endosome
- Early endosome membrane By similarity; Lipid-anchor By similarity
- Endosome membrane By similarity
Cytosol
- cytosol By similarity
Plasma membrane
- Cell membrane By similarity; Lipid-anchor By similarity; Cytoplasmic side By similarity
Other locations
- Melanosome 1 Publication
- Cytoplasmic vesicle By similarity
- ruffle By similarity
- Membrane By similarity
- phagosome membrane 1 Publication
Note: Enriched in stage I melanosomes. Alternates between membrane-bound and cytosolic forms.By similarity
Cytoskeleton
- actin cytoskeleton Source: MGI
Cytosol
- cytosol Source: MGI
Endosome
- cytoplasmic side of early endosome membrane Source: MGI
- early endosome Source: MGI
- endosome Source: UniProtKB
- endosome membrane Source: MGI
- postsynaptic early endosome Source: MGI
- recycling endosome Source: MGI
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- anchored component of synaptic vesicle membrane Source: MGI
- axon Source: ParkinsonsUK-UCL
- axon terminus Source: ParkinsonsUK-UCL
- cytoplasm Source: MGI
- dendrite Source: ParkinsonsUK-UCL
- early phagosome Source: UniProtKB
- endocytic vesicle Source: MGI
- endomembrane system Source: GO_Central
- melanosome Source: UniProtKB-SubCell
- membrane raft Source: MGI
- neuron projection Source: MGI
- neuronal cell body Source: ParkinsonsUK-UCL
- perinuclear region of cytoplasm Source: MGI
- phagocytic vesicle Source: UniProtKB
- phagocytic vesicle membrane Source: UniProtKB-SubCell
- protein-containing complex Source: MGI
- ruffle Source: MGI
- somatodendritic compartment Source: ParkinsonsUK-UCL
- synaptic vesicle Source: ParkinsonsUK-UCL
- terminal bouton Source: ParkinsonsUK-UCL
- zymogen granule membrane Source: MGI
Keywords - Cellular componenti
Cell membrane, Cell projection, Cytoplasm, Cytoplasmic vesicle, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 34 | S → N: Inhibits endosome localization. Disrupts interaction of RAB5A and OCRL with INPP5F. Prevents its localization to apoptotic cell corpse-containing early phagosomes. Maturation of apoptotic cell-containing phagosome into acidic phagosomes is decreased. Interacts with PIK3C3. 2 Publications | 1 | |
Mutagenesisi | 79 | Q → L: Induces the formation of larger endosomes. No effect on interaction with INPP5F. Prevents interaction with DYN2 without affecting the interaction with PIK3C3. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000121106 | 1 – 215 | Ras-related protein Rab-5AAdd BLAST | 215 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 84 | PhosphoserineBy similarity | 1 | |
Lipidationi | 212 | S-geranylgeranyl cysteineBy similarity | 1 | |
Lipidationi | 213 | S-geranylgeranyl cysteineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Phosphoprotein, PrenylationProteomic databases
EPDi | Q9CQD1 |
jPOSTi | Q9CQD1 |
MaxQBi | Q9CQD1 |
PaxDbi | Q9CQD1 |
PeptideAtlasi | Q9CQD1 |
PRIDEi | Q9CQD1 |
PTM databases
iPTMneti | Q9CQD1 |
PhosphoSitePlusi | Q9CQD1 |
SwissPalmi | Q9CQD1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000017831, Expressed in morula and 303 other tissues |
Genevisiblei | Q9CQD1, MM |
Interactioni
Subunit structurei
Interacts with GDI1; this promotes dissociation from membranes; phosphorylation at Ser-84 disrupts this interaction (By similarity).
Interacts with GDI2; phosphorylation at Ser-84 disrupts the interaction (By similarity).
Interacts with EEA1.
Interacts with RIN1 and GAPVD1, which regulate its pathway, probably by acting as a GEF.
Interacts with RINL.
Interacts with ALS2CL, SUN2, ZFYVE20 and RUFY1.
Interacts with RABEP1; one RABEP1 homodimer binds two RAB5A chains, but at opposite sides of the dimer (By similarity).
Interacts with SGSM1 and SGSM3 (PubMed:17509819).
Interacts with PIK3CB (PubMed:21059846).
Interacts with OCRL and INPP5F (PubMed:25869668). May be a component of a complex composed of RAB5A, DYN2 and PIK3C3 (PubMed:18425118). Does not interact with BLOC-3 complex (heterodimer of HPS1 and HPS4).
Interacts with CLN5 (By similarity).
Interacts with APPL2 (PubMed:25568335).
Interacts with F8A1/F8A2/F8A3 (By similarity).
Found in a complex with F8A1/F8A2/F8A3, HTT and RAB5A; mediates the recruitment of HTT by RAB5A onto early endosomes (By similarity).
By similarity1 Publication4 PublicationsGO - Molecular functioni
- GDP-dissociation inhibitor binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 234840, 27 interactors |
IntActi | Q9CQD1, 11 interactors |
MINTi | Q9CQD1 |
STRINGi | 10090.ENSMUSP00000017975 |
Miscellaneous databases
RNActi | Q9CQD1, protein |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 49 – 57 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
GeneTreei | ENSGT00940000154337 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | Q9CQD1 |
OMAi | GKDACNC |
OrthoDBi | 1340129at2759 |
PhylomeDBi | Q9CQD1 |
TreeFami | TF300199 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MANRGATRPN GPNTGNKICQ FKLVLLGESA VGKSSLVLRF VKGQFHEFQE
60 70 80 90 100
STIGAAFLTQ TVCLDDTTVK FEIWDTAGQE RYHSLAPMYY RGAQAAIVVY
110 120 130 140 150
DITNEESFAR AKNWVKELQR QASPNIVIAL SGNKADLANK RAVDFQEAQS
160 170 180 190 200
YADDNSLLFM ETSAKTSMNV NEIFMAIAKK LPKNEPQNPG ANSARGRGVD
210
LTEPAQPARS QCCSN
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 13 | N → D in BAE29485 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 119 | Q → R in BAB22245 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB232593 mRNA Translation: BAF02855.1 AK002631 mRNA Translation: BAB22245.1 AK008198 mRNA Translation: BAB25527.1 AK010495 mRNA Translation: BAB26985.1 AK081999 mRNA Translation: BAC38391.1 AK150296 mRNA Translation: BAE29448.1 AK150346 mRNA Translation: BAE29485.1 AK150405 mRNA Translation: BAE29531.1 AK151066 mRNA Translation: BAE30082.1 AK153131 mRNA Translation: BAE31744.1 AK159706 mRNA Translation: BAE35305.1 CH466559 Genomic DNA Translation: EDL23663.1 BC004842 mRNA Translation: AAH04842.1 BC034370 mRNA Translation: AAH34370.1 BC096481 mRNA Translation: AAH96481.1 |
CCDSi | CCDS28879.1 |
RefSeqi | NP_080163.1, NM_025887.4 |
Genome annotation databases
Ensembli | ENSMUST00000017975; ENSMUSP00000017975; ENSMUSG00000017831 |
GeneIDi | 271457 |
KEGGi | mmu:271457 |
UCSCi | uc008czn.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB232593 mRNA Translation: BAF02855.1 AK002631 mRNA Translation: BAB22245.1 AK008198 mRNA Translation: BAB25527.1 AK010495 mRNA Translation: BAB26985.1 AK081999 mRNA Translation: BAC38391.1 AK150296 mRNA Translation: BAE29448.1 AK150346 mRNA Translation: BAE29485.1 AK150405 mRNA Translation: BAE29531.1 AK151066 mRNA Translation: BAE30082.1 AK153131 mRNA Translation: BAE31744.1 AK159706 mRNA Translation: BAE35305.1 CH466559 Genomic DNA Translation: EDL23663.1 BC004842 mRNA Translation: AAH04842.1 BC034370 mRNA Translation: AAH34370.1 BC096481 mRNA Translation: AAH96481.1 |
CCDSi | CCDS28879.1 |
RefSeqi | NP_080163.1, NM_025887.4 |
3D structure databases
SMRi | Q9CQD1 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 234840, 27 interactors |
IntActi | Q9CQD1, 11 interactors |
MINTi | Q9CQD1 |
STRINGi | 10090.ENSMUSP00000017975 |
PTM databases
iPTMneti | Q9CQD1 |
PhosphoSitePlusi | Q9CQD1 |
SwissPalmi | Q9CQD1 |
Proteomic databases
EPDi | Q9CQD1 |
jPOSTi | Q9CQD1 |
MaxQBi | Q9CQD1 |
PaxDbi | Q9CQD1 |
PeptideAtlasi | Q9CQD1 |
PRIDEi | Q9CQD1 |
Protocols and materials databases
Antibodypediai | 3882, 725 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000017975; ENSMUSP00000017975; ENSMUSG00000017831 |
GeneIDi | 271457 |
KEGGi | mmu:271457 |
UCSCi | uc008czn.1, mouse |
Organism-specific databases
CTDi | 5868 |
MGIi | MGI:105926, Rab5a |
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
GeneTreei | ENSGT00940000154337 |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | Q9CQD1 |
OMAi | GKDACNC |
OrthoDBi | 1340129at2759 |
PhylomeDBi | Q9CQD1 |
TreeFami | TF300199 |
Enzyme and pathway databases
Reactomei | R-MMU-1660499, Synthesis of PIPs at the plasma membrane R-MMU-8856828, Clathrin-mediated endocytosis R-MMU-8873719, RAB geranylgeranylation R-MMU-8876198, RAB GEFs exchange GTP for GDP on RABs R-MMU-983231, Factors involved in megakaryocyte development and platelet production |
Miscellaneous databases
BioGRID-ORCSi | 271457, 1 hit in 19 CRISPR screens |
ChiTaRSi | Rab5a, mouse |
PROi | PR:Q9CQD1 |
RNActi | Q9CQD1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000017831, Expressed in morula and 303 other tissues |
Genevisiblei | Q9CQD1, MM |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RAB5A_MOUSE | |
Accessioni | Q9CQD1Primary (citable) accession number: Q9CQD1 Secondary accession number(s): Q3UCX7, Q4VAA1, Q9DCN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 173 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families