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Protein

Mediator of RNA polymerase II transcription subunit 4

Gene

Med4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActivator
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Mediator of RNA polymerase II transcription subunit 4
Alternative name(s):
Mediator complex subunit 4
Gene namesi
Name:Med4
Synonyms:Vdrip
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 14

Organism-specific databases

MGIiMGI:1914631 Med4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000963842 – 270Mediator of RNA polymerase II transcription subunit 4Add BLAST269

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei32PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9CQA5
PaxDbiQ9CQA5
PeptideAtlasiQ9CQA5
PRIDEiQ9CQA5

PTM databases

iPTMnetiQ9CQA5
PhosphoSitePlusiQ9CQA5

Expressioni

Gene expression databases

BgeeiENSMUSG00000022109 Expressed in 269 organ(s), highest expression level in dorsal pancreas
GenevisibleiQ9CQA5 MM

Interactioni

Subunit structurei

Component of the Mediator complex, which is composed of MED1, MED4, MED6, MED7, MED8, MED9, MED10, MED11, MED12, MED13, MED13L, MED14, MED15, MED16, MED17, MED18, MED19, MED20, MED21, MED22, MED23, MED24, MED25, MED26, MED27, MED29, MED30, MED31, CCNC, CDK8 and CDC2L6/CDK11. The MED12, MED13, CCNC and CDK8 subunits form a distinct module termed the CDK8 module. Mediator containing the CDK8 module is less active than Mediator lacking this module in supporting transcriptional activation. Individual preparations of the Mediator complex lacking one or more distinct subunits have been variously termed ARC, CRSP, DRIP, PC2, SMCC and TRAP (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi212147, 2 interactors
ComplexPortaliCPX-3264 Core mediator complex
CORUMiQ9CQA5
IntActiQ9CQA5, 5 interactors
MINTiQ9CQA5
STRINGi10090.ENSMUSP00000022705

Structurei

3D structure databases

SMRiQ9CQA5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili26 – 131Sequence analysisAdd BLAST106

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi262 – 269Poly-Ser8

Sequence similaritiesi

Belongs to the Mediator complex subunit 4 family.Curated

Keywords - Domaini

Coiled coil

Phylogenomic databases

eggNOGiKOG4552 Eukaryota
ENOG410XQ30 LUCA
GeneTreeiENSGT00390000012063
HOGENOMiHOG000043883
HOVERGENiHBG052450
InParanoidiQ9CQA5
KOiK15146
OMAiPPHHGND
OrthoDBiEOG091G0M8S
PhylomeDBiQ9CQA5
TreeFamiTF324421

Family and domain databases

InterProiView protein in InterPro
IPR019258 Mediator_Med4
PANTHERiPTHR13208 PTHR13208, 1 hit
PfamiView protein in Pfam
PF10018 Med4, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9CQA5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MAASSSGEKE KERMGGVSGM AGLGSTRERL LSALEDLEVL SRELIEMLAI
60 70 80 90 100
SRNQKLLQLE EENQVLELLI HRDGDFQELM KLALNQGKVH HEMQALEKEV
110 120 130 140 150
EKRDSDIQQL QKQLKEAEQI LATAVYQAKE KLKSIEKARK GAISSEEIIK
160 170 180 190 200
YAHRISASNA VCAPLTWVPG DPRRPYPTDL EMRSGLLGQM NNPSTSGVNG
210 220 230 240 250
HLPGDALAAG RLPDVLAPQY PWQSNDMSVN MLPPNHSSDF LLEPPGHNKE
260 270
NEDDVEVMST DSSSSSSDSD
Length:270
Mass (Da):29,781
Last modified:June 1, 2001 - v1
Checksum:iA827D43766AAAD52
GO
Isoform 2 (identifier: Q9CQA5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     171-177: DPRRPYP → NRASYIR
     178-270: Missing.

Note: No experimental confirmation available.
Show »
Length:177
Mass (Da):19,868
Checksum:iBD12B395306DDDB9
GO

Sequence cautioni

The sequence BAE26554 differs from that shown. Reason: Erroneous initiation.Curated

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027918171 – 177DPRRPYP → NRASYIR in isoform 2. 1 Publication7
Alternative sequenceiVSP_027919178 – 270Missing in isoform 2. 1 PublicationAdd BLAST93

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010270 mRNA Translation: BAB26809.1
AK010686 mRNA Translation: BAB27118.1
AK078288 mRNA Translation: BAC37206.1
AK145636 mRNA Translation: BAE26554.1 Different initiation.
BC024950 mRNA Translation: AAH24950.1
CCDSiCCDS49538.1 [Q9CQA5-1]
RefSeqiNP_080395.1, NM_026119.3 [Q9CQA5-1]
UniGeneiMm.282888

Genome annotation databases

EnsembliENSMUST00000022705; ENSMUSP00000022705; ENSMUSG00000022109 [Q9CQA5-1]
GeneIDi67381
KEGGimmu:67381
UCSCiuc007upu.1 mouse [Q9CQA5-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK010270 mRNA Translation: BAB26809.1
AK010686 mRNA Translation: BAB27118.1
AK078288 mRNA Translation: BAC37206.1
AK145636 mRNA Translation: BAE26554.1 Different initiation.
BC024950 mRNA Translation: AAH24950.1
CCDSiCCDS49538.1 [Q9CQA5-1]
RefSeqiNP_080395.1, NM_026119.3 [Q9CQA5-1]
UniGeneiMm.282888

3D structure databases

SMRiQ9CQA5
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi212147, 2 interactors
ComplexPortaliCPX-3264 Core mediator complex
CORUMiQ9CQA5
IntActiQ9CQA5, 5 interactors
MINTiQ9CQA5
STRINGi10090.ENSMUSP00000022705

PTM databases

iPTMnetiQ9CQA5
PhosphoSitePlusiQ9CQA5

Proteomic databases

EPDiQ9CQA5
PaxDbiQ9CQA5
PeptideAtlasiQ9CQA5
PRIDEiQ9CQA5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000022705; ENSMUSP00000022705; ENSMUSG00000022109 [Q9CQA5-1]
GeneIDi67381
KEGGimmu:67381
UCSCiuc007upu.1 mouse [Q9CQA5-1]

Organism-specific databases

CTDi29079
MGIiMGI:1914631 Med4

Phylogenomic databases

eggNOGiKOG4552 Eukaryota
ENOG410XQ30 LUCA
GeneTreeiENSGT00390000012063
HOGENOMiHOG000043883
HOVERGENiHBG052450
InParanoidiQ9CQA5
KOiK15146
OMAiPPHHGND
OrthoDBiEOG091G0M8S
PhylomeDBiQ9CQA5
TreeFamiTF324421

Enzyme and pathway databases

ReactomeiR-MMU-212436 Generic Transcription Pathway

Miscellaneous databases

PROiPR:Q9CQA5
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022109 Expressed in 269 organ(s), highest expression level in dorsal pancreas
GenevisibleiQ9CQA5 MM

Family and domain databases

InterProiView protein in InterPro
IPR019258 Mediator_Med4
PANTHERiPTHR13208 PTHR13208, 1 hit
PfamiView protein in Pfam
PF10018 Med4, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiMED4_MOUSE
AccessioniPrimary (citable) accession number: Q9CQA5
Secondary accession number(s): Q3UL96, Q8BVG6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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