UniProtKB - Q9CQ65 (MTAP_MOUSE)
Protein
S-methyl-5'-thioadenosine phosphorylase
Gene
Mtap
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the reversible phosphorylation of S-methyl-5'-thioadenosine (MTA) to adenine and 5-methylthioribose-1-phosphate. Involved in the breakdown of MTA, a major by-product of polyamine biosynthesis. Responsible for the first step in the methionine salvage pathway after MTA has been generated from S-adenosylmethionine. Has broad substrate specificity with 6-aminopurine nucleosides as preferred substrates.UniRule annotation
Catalytic activityi
- phosphate + S-methyl-5'-thioadenosine = adenine + S-methyl-5-thio-α-D-ribose 1-phosphateUniRule annotationEC:2.4.2.28UniRule annotation
: L-methionine biosynthesis via salvage pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route).UniRule annotationProteins known to be involved in this subpathway in this organism are:
- S-methyl-5'-thioadenosine phosphorylase (Mtap)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes S-methyl-5-thio-alpha-D-ribose 1-phosphate from S-methyl-5'-thioadenosine (phosphorylase route), the pathway L-methionine biosynthesis via salvage pathway and in Amino-acid biosynthesis.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 18 | PhosphateUniRule annotation | 1 | |
Sitei | 178 | Important for substrate specificityUniRule annotation | 1 | |
Binding sitei | 196 | Substrate; via amide nitrogenUniRule annotation | 1 | |
Binding sitei | 197 | PhosphateUniRule annotation | 1 | |
Sitei | 233 | Important for substrate specificityUniRule annotation | 1 |
GO - Molecular functioni
- purine-nucleoside phosphorylase activity Source: GO_Central
- S-methyl-5-thioadenosine phosphorylase activity Source: GO_Central
GO - Biological processi
- L-methionine salvage from methylthioadenosine Source: GO_Central
- methylation Source: MGI
- purine ribonucleoside salvage Source: UniProtKB-KW
Keywordsi
Molecular function | Glycosyltransferase, Transferase |
Biological process | Purine salvage |
Enzyme and pathway databases
BRENDAi | 2.4.2.28, 3474 |
Reactomei | R-MMU-1237112, Methionine salvage pathway |
UniPathwayi | UPA00904;UER00873 |
Names & Taxonomyi
Protein namesi | Recommended name: S-methyl-5'-thioadenosine phosphorylaseUniRule annotation (EC:2.4.2.28UniRule annotation)Alternative name(s): 5'-methylthioadenosine phosphorylaseUniRule annotation Short name: MTA phosphorylaseUniRule annotation Short name: MTAPUniRule annotation Short name: MTAPaseUniRule annotation |
Gene namesi | Name:Mtap |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914152, Mtap |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000184546 | 1 – 283 | S-methyl-5'-thioadenosine phosphorylaseAdd BLAST | 283 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 51 | N6-acetyllysineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
EPDi | Q9CQ65 |
jPOSTi | Q9CQ65 |
MaxQBi | Q9CQ65 |
PaxDbi | Q9CQ65 |
PeptideAtlasi | Q9CQ65 |
PRIDEi | Q9CQ65 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9CQ65 |
PTM databases
iPTMneti | Q9CQ65 |
PhosphoSitePlusi | Q9CQ65 |
SwissPalmi | Q9CQ65 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000062937, Expressed in metanephric ureteric bud and 322 other tissues |
Genevisiblei | Q9CQ65, MM |
Interactioni
Subunit structurei
Homotrimer.
UniRule annotationProtein-protein interaction databases
BioGRIDi | 211799, 18 interactors |
IntActi | Q9CQ65, 2 interactors |
MINTi | Q9CQ65 |
STRINGi | 10090.ENSMUSP00000061092 |
Chemistry databases
BindingDBi | Q9CQ65 |
Miscellaneous databases
RNActi | Q9CQ65, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 60 – 61 | Phosphate bindingUniRule annotation | 2 | |
Regioni | 93 – 94 | Phosphate bindingUniRule annotation | 2 | |
Regioni | 220 – 222 | Substrate bindingUniRule annotation | 3 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3985, Eukaryota |
GeneTreei | ENSGT00950000182991 |
HOGENOMi | CLU_054456_0_0_1 |
InParanoidi | Q9CQ65 |
OMAi | DYDVWAE |
OrthoDBi | 1616485at2759 |
PhylomeDBi | Q9CQ65 |
TreeFami | TF312883 |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
HAMAPi | MF_01963, MTAP, 1 hit |
InterProi | View protein in InterPro IPR010044, MTAP IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR018099, Purine_phosphorylase-2_CS |
PANTHERi | PTHR42679, PTHR42679, 1 hit |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01694, MTAP, 1 hit |
PROSITEi | View protein in PROSITE PS01240, PNP_MTAP_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9CQ65-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MASGSACTAV KIGIIGGTGL DDPEILEGRT EKYVDTPFGK PSDALILGKI
60 70 80 90 100
KNVDCVLLAR HGRQHTIMPS KVNYQANIWA LKEEGCTHVI VTTACGSLRE
110 120 130 140 150
EIQPGDMVII DQFIDRTSLR PQTFYDGSHC SARGVCHIPM AEPFCPKTRE
160 170 180 190 200
VLIETAKKLG LRCHSKGTIV TIEGPRFSSR AESLIFRTWG ADVVNMTTVP
210 220 230 240 250
EVVLAKEAGI CYASIAMATD YDCWKEHEEA VSVDGVLKTM KENANKAKSL
260 270 280
LLTTIPQIGS MEWSETLRNL KNMAQFSVLP PRH
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB056100 mRNA Translation: BAB32865.1 AK005064 mRNA Translation: BAB23788.1 AK011421 mRNA Translation: BAB27609.1 AK167319 mRNA Translation: BAE39421.1 BC003858 mRNA Translation: AAH03858.1 |
CCDSi | CCDS18349.1 |
RefSeqi | NP_077753.1, NM_024433.2 |
Genome annotation databases
Ensembli | ENSMUST00000058030; ENSMUSP00000061092; ENSMUSG00000062937 |
GeneIDi | 66902 |
KEGGi | mmu:66902 |
UCSCi | uc008tof.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB056100 mRNA Translation: BAB32865.1 AK005064 mRNA Translation: BAB23788.1 AK011421 mRNA Translation: BAB27609.1 AK167319 mRNA Translation: BAE39421.1 BC003858 mRNA Translation: AAH03858.1 |
CCDSi | CCDS18349.1 |
RefSeqi | NP_077753.1, NM_024433.2 |
3D structure databases
SMRi | Q9CQ65 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211799, 18 interactors |
IntActi | Q9CQ65, 2 interactors |
MINTi | Q9CQ65 |
STRINGi | 10090.ENSMUSP00000061092 |
Chemistry databases
BindingDBi | Q9CQ65 |
ChEMBLi | CHEMBL2663 |
PTM databases
iPTMneti | Q9CQ65 |
PhosphoSitePlusi | Q9CQ65 |
SwissPalmi | Q9CQ65 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9CQ65 |
Proteomic databases
EPDi | Q9CQ65 |
jPOSTi | Q9CQ65 |
MaxQBi | Q9CQ65 |
PaxDbi | Q9CQ65 |
PeptideAtlasi | Q9CQ65 |
PRIDEi | Q9CQ65 |
Protocols and materials databases
Antibodypediai | 35188, 255 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000058030; ENSMUSP00000061092; ENSMUSG00000062937 |
GeneIDi | 66902 |
KEGGi | mmu:66902 |
UCSCi | uc008tof.2, mouse |
Organism-specific databases
CTDi | 4507 |
MGIi | MGI:1914152, Mtap |
Phylogenomic databases
eggNOGi | KOG3985, Eukaryota |
GeneTreei | ENSGT00950000182991 |
HOGENOMi | CLU_054456_0_0_1 |
InParanoidi | Q9CQ65 |
OMAi | DYDVWAE |
OrthoDBi | 1616485at2759 |
PhylomeDBi | Q9CQ65 |
TreeFami | TF312883 |
Enzyme and pathway databases
UniPathwayi | UPA00904;UER00873 |
BRENDAi | 2.4.2.28, 3474 |
Reactomei | R-MMU-1237112, Methionine salvage pathway |
Miscellaneous databases
BioGRID-ORCSi | 66902, 0 hits in 17 CRISPR screens |
ChiTaRSi | Mtap, mouse |
PROi | PR:Q9CQ65 |
RNActi | Q9CQ65, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000062937, Expressed in metanephric ureteric bud and 322 other tissues |
Genevisiblei | Q9CQ65, MM |
Family and domain databases
Gene3Di | 3.40.50.1580, 1 hit |
HAMAPi | MF_01963, MTAP, 1 hit |
InterProi | View protein in InterPro IPR010044, MTAP IPR000845, Nucleoside_phosphorylase_d IPR035994, Nucleoside_phosphorylase_sf IPR018099, Purine_phosphorylase-2_CS |
PANTHERi | PTHR42679, PTHR42679, 1 hit |
Pfami | View protein in Pfam PF01048, PNP_UDP_1, 1 hit |
SUPFAMi | SSF53167, SSF53167, 1 hit |
TIGRFAMsi | TIGR01694, MTAP, 1 hit |
PROSITEi | View protein in PROSITE PS01240, PNP_MTAP_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | MTAP_MOUSE | |
Accessioni | Q9CQ65Primary (citable) accession number: Q9CQ65 Secondary accession number(s): Q3TJS4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 24, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 149 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families