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Protein

Cytosol aminopeptidase

Gene

Lap3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Presumably involved in the processing and regular turnover of intracellular proteins. Catalyzes the removal of unsubstituted N-terminal amino acids from various peptides (By similarity).By similarity

Catalytic activityi

Release of an N-terminal amino acid, Xaa-|-Yaa-, in which Xaa is preferably Leu, but may be other amino acids including Pro although not Arg or Lys, and Yaa may be Pro. Amino acid amides and methyl esters are also readily hydrolyzed, but rates on arylamides are exceedingly low.
Release of N-terminal proline from a peptide.

Cofactori

Note: Binds 2 zinc ions per subunit. One zinc ion is tightly bound and essential for enzyme activity, while the second metal coordination site can be occupied by zinc, magnesium or manganese to give enzymes of different activities.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi282Zinc 2By similarity1
Metal bindingi287Zinc 1By similarity1
Metal bindingi287Zinc 2By similarity1
Active sitei294By similarity1
Metal bindingi305Zinc 2By similarity1
Metal bindingi364Zinc 1By similarity1
Metal bindingi366Zinc 1By similarity1
Metal bindingi366Zinc 2By similarity1
Active sitei368By similarity1

GO - Molecular functioni

Keywordsi

Molecular functionAminopeptidase, Hydrolase, Protease
LigandMagnesium, Manganese, Metal-binding, Zinc

Protein family/group databases

MEROPSiM17.001

Names & Taxonomyi

Protein namesi
Recommended name:
Cytosol aminopeptidase (EC:3.4.11.1)
Alternative name(s):
Leucine aminopeptidase 3
Short name:
LAP-3
Leucyl aminopeptidase
Proline aminopeptidase (EC:3.4.11.5)
Prolyl aminopeptidase
Gene namesi
Name:Lap3
Synonyms:Lapep
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1914238 Lap3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001658261 – 519Cytosol aminopeptidaseAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei45N6-succinyllysineCombined sources1
Modified residuei54PhosphoserineBy similarity1
Modified residuei61N6-succinyllysineCombined sources1
Modified residuei103N6-succinyllysineCombined sources1
Modified residuei180PhosphoserineCombined sources1
Modified residuei194PhosphoserineBy similarity1
Modified residuei221N6-acetyllysine; alternateCombined sources1
Modified residuei221N6-succinyllysine; alternateCombined sources1
Modified residuei238PhosphoserineCombined sources1
Modified residuei455N6-acetyllysine; alternateCombined sources1
Modified residuei455N6-succinyllysine; alternateCombined sources1
Modified residuei476N6-succinyllysineCombined sources1
Modified residuei489N6-acetyllysine; alternateCombined sources1
Modified residuei489N6-succinyllysine; alternateCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9CPY7
PeptideAtlasiQ9CPY7
PRIDEiQ9CPY7

2D gel databases

REPRODUCTION-2DPAGEiIPI00828469

PTM databases

iPTMnetiQ9CPY7
PhosphoSitePlusiQ9CPY7
SwissPalmiQ9CPY7

Expressioni

Gene expression databases

BgeeiENSMUSG00000039682 Expressed in 311 organ(s), highest expression level in white adipose tissue
CleanExiMM_LAP3
ExpressionAtlasiQ9CPY7 baseline and differential
GenevisibleiQ9CPY7 MM

Interactioni

Subunit structurei

Homohexamer.By similarity

Protein-protein interaction databases

IntActiQ9CPY7, 5 interactors
MINTiQ9CPY7
STRINGi10090.ENSMUSP00000040222

Structurei

3D structure databases

ProteinModelPortaliQ9CPY7
SMRiQ9CPY7
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase M17 family.Curated

Phylogenomic databases

eggNOGiKOG2597 Eukaryota
COG0260 LUCA
GeneTreeiENSGT00530000063255
HOGENOMiHOG000243129
HOVERGENiHBG003320
InParanoidiQ9CPY7
KOiK11142
OMAiMKNTGPR
OrthoDBiEOG091G0ERB
PhylomeDBiQ9CPY7
TreeFamiTF314954

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9CPY7-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MYLLPLPAAA RVALRRLGVR GLWDRGLSTA DMTKGLVLGI YAKDKDDDLP
60 70 80 90 100
QFTSAGESFN KLVSGKLREM LNISGPPLKA GKTRTFYGLH QDFPSVVVVG
110 120 130 140 150
LGKRSAGVDD QENWHEGKEN IRAAVAAGCR QVQDLELPSV EVDPCGDAQA
160 170 180 190 200
AAEGAVLGLY EYDDLKQKKK VAVSAKLHGS GDLEAWEKGV LFASGQNLAR
210 220 230 240 250
HLMESPANEM TPTRFAEIIE KNLKSASSKT KVHIRPKSWI EEQEMGSFLS
260 270 280 290 300
VAKGSEEPPV FLEIHYMGSP NATEAPLVFV GKGITFDSGG ISIKASANMD
310 320 330 340 350
LMRADMGGAA TICSAIVSAA KLNLPINIIG LAPLCENMPS GKANKPGDVV
360 370 380 390 400
RARNGKTIQV DNTDAEGRLI LADALCYAHT FNPKVIINAA TLTGAMDVAL
410 420 430 440 450
GSGATGVFTN SSWLWNKLFE ASVETGDRVW RMPLFEHYTR QVIDCQLADV
460 470 480 490 500
NNLGKYRSAG ACTAAAFLRE FVTHTKWAHL DIAGVMTNKD EIPYLRKGMS
510
GRPTRTLIEF LLRFSKDSS
Length:519
Mass (Da):56,141
Last modified:January 23, 2007 - v3
Checksum:i46729A9CE4C060A5
GO
Isoform 2 (identifier: Q9CPY7-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-31: Missing.

Note: No experimental confirmation available.
Show »
Length:488
Mass (Da):52,750
Checksum:iE6800977EC37A2AC
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A0G2JEM7A0A0G2JEM7_MOUSE
Cytosol aminopeptidase
Lap3
107Annotation score:

Sequence cautioni

The sequence BAB23958 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB25769 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAB27726 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE39141 differs from that shown. Reason: Erroneous initiation.Curated
The sequence BAE40823 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti46D → G in CAJ18509 (Ref. 2) Curated1
Sequence conflicti93F → L in CAJ18445 (Ref. 2) Curated1
Sequence conflicti123A → D in BAB26987 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0226321 – 31Missing in isoform 2. CuratedAdd BLAST31

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334160 mRNA Translation: AAK13495.1
CT010237 mRNA Translation: CAJ18445.1
CT010301 mRNA Translation: CAJ18509.1
AK002819 mRNA No translation available.
AK005334 mRNA Translation: BAB23958.1 Different initiation.
AK008600 mRNA Translation: BAB25769.1 Different initiation.
AK010502 mRNA Translation: BAB26987.1
AK011604 mRNA Translation: BAB27726.1 Different initiation.
AK166958 mRNA Translation: BAE39141.1 Different initiation.
AK169032 mRNA Translation: BAE40823.1 Different initiation.
BC016536 mRNA Translation: AAH16536.1
CCDSiCCDS19274.1 [Q9CPY7-1]
RefSeqiNP_077754.3, NM_024434.6 [Q9CPY7-1]
UniGeneiMm.286830

Genome annotation databases

EnsembliENSMUST00000046122; ENSMUSP00000040222; ENSMUSG00000039682 [Q9CPY7-1]
GeneIDi66988
KEGGimmu:66988
UCSCiuc008xiy.2 mouse [Q9CPY7-1]

Keywords - Coding sequence diversityi

Alternative initiation

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF334160 mRNA Translation: AAK13495.1
CT010237 mRNA Translation: CAJ18445.1
CT010301 mRNA Translation: CAJ18509.1
AK002819 mRNA No translation available.
AK005334 mRNA Translation: BAB23958.1 Different initiation.
AK008600 mRNA Translation: BAB25769.1 Different initiation.
AK010502 mRNA Translation: BAB26987.1
AK011604 mRNA Translation: BAB27726.1 Different initiation.
AK166958 mRNA Translation: BAE39141.1 Different initiation.
AK169032 mRNA Translation: BAE40823.1 Different initiation.
BC016536 mRNA Translation: AAH16536.1
CCDSiCCDS19274.1 [Q9CPY7-1]
RefSeqiNP_077754.3, NM_024434.6 [Q9CPY7-1]
UniGeneiMm.286830

3D structure databases

ProteinModelPortaliQ9CPY7
SMRiQ9CPY7
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9CPY7, 5 interactors
MINTiQ9CPY7
STRINGi10090.ENSMUSP00000040222

Protein family/group databases

MEROPSiM17.001

PTM databases

iPTMnetiQ9CPY7
PhosphoSitePlusiQ9CPY7
SwissPalmiQ9CPY7

2D gel databases

REPRODUCTION-2DPAGEiIPI00828469

Proteomic databases

PaxDbiQ9CPY7
PeptideAtlasiQ9CPY7
PRIDEiQ9CPY7

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000046122; ENSMUSP00000040222; ENSMUSG00000039682 [Q9CPY7-1]
GeneIDi66988
KEGGimmu:66988
UCSCiuc008xiy.2 mouse [Q9CPY7-1]

Organism-specific databases

CTDi51056
MGIiMGI:1914238 Lap3

Phylogenomic databases

eggNOGiKOG2597 Eukaryota
COG0260 LUCA
GeneTreeiENSGT00530000063255
HOGENOMiHOG000243129
HOVERGENiHBG003320
InParanoidiQ9CPY7
KOiK11142
OMAiMKNTGPR
OrthoDBiEOG091G0ERB
PhylomeDBiQ9CPY7
TreeFamiTF314954

Miscellaneous databases

ChiTaRSiLap3 mouse
PROiPR:Q9CPY7
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000039682 Expressed in 311 organ(s), highest expression level in white adipose tissue
CleanExiMM_LAP3
ExpressionAtlasiQ9CPY7 baseline and differential
GenevisibleiQ9CPY7 MM

Family and domain databases

CDDicd00433 Peptidase_M17, 1 hit
HAMAPiMF_00181 Cytosol_peptidase_M17, 1 hit
InterProiView protein in InterPro
IPR011356 Leucine_aapep/pepB
IPR000819 Peptidase_M17_C
IPR023042 Peptidase_M17_leu_NH2_pept
IPR008283 Peptidase_M17_N
PANTHERiPTHR11963 PTHR11963, 1 hit
PfamiView protein in Pfam
PF00883 Peptidase_M17, 1 hit
PF02789 Peptidase_M17_N, 1 hit
PRINTSiPR00481 LAMNOPPTDASE
PROSITEiView protein in PROSITE
PS00631 CYTOSOL_AP, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiAMPL_MOUSE
AccessioniPrimary (citable) accession number: Q9CPY7
Secondary accession number(s): Q3TFS5
, Q4FJV1, Q4FK15, Q99P44, Q9CWN8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: January 23, 2007
Last modified: September 12, 2018
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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