UniProtKB - Q9CPW4 (ARPC5_MOUSE)
Protein
Actin-related protein 2/3 complex subunit 5
Gene
Arpc5
Organism
Mus musculus (Mouse)
Status
Functioni
Component of the Arp2/3 complex, a multiprotein complex that mediates actin polymerization upon stimulation by nucleation-promoting factor (NPF). The Arp2/3 complex mediates the formation of branched actin networks in the cytoplasm, providing the force for cell motility. In addition to its role in the cytoplasmic cytoskeleton, the Arp2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA. The Arp2/3 complex promotes homologous recombination (HR) repair in response to DNA damage by promoting nuclear actin polymerization, leading to drive motility of double-strand breaks (DSBs).By similarity
GO - Molecular functioni
- actin filament binding Source: GO_Central
- structural constituent of cytoskeleton Source: MGI
GO - Biological processi
- actin filament network formation Source: MGI
- Arp2/3 complex-mediated actin nucleation Source: MGI
- cell migration Source: MGI
- lamellipodium organization Source: MGI
- maintenance of cell polarity Source: MGI
- microtubule organizing center localization Source: MGI
- orbitofrontal cortex development Source: Ensembl
- smooth muscle cell migration Source: MGI
Keywordsi
Molecular function | Actin-binding |
Enzyme and pathway databases
Reactomei | R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-3928662, EPHB-mediated forward signaling R-MMU-5663213, RHO GTPases Activate WASPs and WAVEs R-MMU-6798695, Neutrophil degranulation R-MMU-8856828, Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: Actin-related protein 2/3 complex subunit 5Alternative name(s): Arp2/3 complex 16 kDa subunit Short name: p16-ARC |
Gene namesi | Name:Arpc5 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1915021, Arpc5 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Cytoskeleton
- cytoskeleton By similarity
Other locations
- Cell projection By similarity
Cytoskeleton
- Arp2/3 protein complex Source: UniProtKB
Endosome
- endosome Source: MGI
Nucleus
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: GO_Central
- growth cone Source: MGI
- lamellipodium Source: MGI
- neuron projection Source: MGI
- site of double-strand break Source: UniProtKB
Keywords - Cellular componenti
Cell projection, Cytoplasm, Cytoskeleton, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000124055 | 2 – 151 | Actin-related protein 2/3 complex subunit 5Add BLAST | 150 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 |
Post-translational modificationi
Polyubiquitinated by RNF128 with 'Lys-63'-linked chains, leading to proteasomal degradation.By similarity
Keywords - PTMi
Acetylation, Ubl conjugationProteomic databases
EPDi | Q9CPW4 |
jPOSTi | Q9CPW4 |
MaxQBi | Q9CPW4 |
PaxDbi | Q9CPW4 |
PRIDEi | Q9CPW4 |
TopDownProteomicsi | Q9CPW4 |
PTM databases
iPTMneti | Q9CPW4 |
PhosphoSitePlusi | Q9CPW4 |
SwissPalmi | Q9CPW4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000008475, Expressed in granulocyte and 290 other tissues |
Genevisiblei | Q9CPW4, MM |
Interactioni
Subunit structurei
Component of the Arp2/3 complex composed of ACTR2/ARP2, ACTR3/ARP3, ARPC1B/p41-ARC, ARPC2/p34-ARC, ARPC3/p21-ARC, ARPC4/p20-ARC and ARPC5/p16-ARC.
By similarityGO - Molecular functioni
- actin filament binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 212431, 15 interactors |
DIPi | DIP-60589N |
IntActi | Q9CPW4, 3 interactors |
STRINGi | 10090.ENSMUSP00000076933 |
Miscellaneous databases
RNActi | Q9CPW4, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the ARPC5 family.Curated
Phylogenomic databases
eggNOGi | KOG3380, Eukaryota |
GeneTreei | ENSGT00940000154654 |
HOGENOMi | CLU_101888_1_1_1 |
InParanoidi | Q9CPW4 |
OMAi | GMALPGW |
OrthoDBi | 1565115at2759 |
PhylomeDBi | Q9CPW4 |
TreeFami | TF319716 |
Family and domain databases
Gene3Di | 1.25.40.190, 1 hit |
InterProi | View protein in InterPro IPR006789, ARPC5 IPR036743, ARPC5_sf |
PANTHERi | PTHR12644, PTHR12644, 1 hit |
Pfami | View protein in Pfam PF04699, P16-Arc, 1 hit |
PIRSFi | PIRSF039096, p16-ARC, 1 hit |
SUPFAMi | SSF69103, SSF69103, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9CPW4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSKNTVSSAR FRKVDVDEYD ENKFVDEEDG GDGQAGPDEG EVDSCLRQGN
60 70 80 90 100
MTAALQAALK NPPINTKSQA VKDRAGSIVL KVLISFKAND IEKAVQSLDK
110 120 130 140 150
NGVDLLMKYI YKGFESPSDN SSAVLLQWHE KALAAGGVGS IVRVLTARKT
V
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ3UA72 | Q3UA72_MOUSE | Actin-related protein 2/3 complex s... | Arpc5 | 92 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 59 | L → Q in BAB25457 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008097 mRNA Translation: BAB25457.1 AK011348 mRNA Translation: BAB27558.1 AK011911 mRNA Translation: BAB27911.1 AK017669 mRNA Translation: BAB30864.1 AK019149 mRNA Translation: BAB31570.1 AK087427 mRNA Translation: BAC39870.1 AK149756 mRNA Translation: BAE29064.1 AK151654 mRNA Translation: BAE30583.1 AK151656 mRNA Translation: BAE30585.1 AK152281 mRNA Translation: BAE31095.1 AK153262 mRNA Translation: BAE31852.1 AK159407 mRNA Translation: BAE35058.1 AK159438 mRNA Translation: BAE35084.1 AK159738 mRNA Translation: BAE35332.1 AK166284 mRNA Translation: BAE38681.1 AK166335 mRNA Translation: BAE38714.1 AK166725 mRNA Translation: BAE38972.1 AK167916 mRNA Translation: BAE39923.1 AK168264 mRNA Translation: BAE40212.1 AK168986 mRNA Translation: BAE40786.1 BC060143 mRNA Translation: AAH60143.1 |
CCDSi | CCDS35739.1 |
RefSeqi | NP_080645.2, NM_026369.2 |
Genome annotation databases
Ensembli | ENSMUST00000077755; ENSMUSP00000076933; ENSMUSG00000008475 |
GeneIDi | 67771 |
KEGGi | mmu:67771 |
UCSCi | uc007czj.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK008097 mRNA Translation: BAB25457.1 AK011348 mRNA Translation: BAB27558.1 AK011911 mRNA Translation: BAB27911.1 AK017669 mRNA Translation: BAB30864.1 AK019149 mRNA Translation: BAB31570.1 AK087427 mRNA Translation: BAC39870.1 AK149756 mRNA Translation: BAE29064.1 AK151654 mRNA Translation: BAE30583.1 AK151656 mRNA Translation: BAE30585.1 AK152281 mRNA Translation: BAE31095.1 AK153262 mRNA Translation: BAE31852.1 AK159407 mRNA Translation: BAE35058.1 AK159438 mRNA Translation: BAE35084.1 AK159738 mRNA Translation: BAE35332.1 AK166284 mRNA Translation: BAE38681.1 AK166335 mRNA Translation: BAE38714.1 AK166725 mRNA Translation: BAE38972.1 AK167916 mRNA Translation: BAE39923.1 AK168264 mRNA Translation: BAE40212.1 AK168986 mRNA Translation: BAE40786.1 BC060143 mRNA Translation: AAH60143.1 |
CCDSi | CCDS35739.1 |
RefSeqi | NP_080645.2, NM_026369.2 |
3D structure databases
SMRi | Q9CPW4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 212431, 15 interactors |
DIPi | DIP-60589N |
IntActi | Q9CPW4, 3 interactors |
STRINGi | 10090.ENSMUSP00000076933 |
PTM databases
iPTMneti | Q9CPW4 |
PhosphoSitePlusi | Q9CPW4 |
SwissPalmi | Q9CPW4 |
Proteomic databases
EPDi | Q9CPW4 |
jPOSTi | Q9CPW4 |
MaxQBi | Q9CPW4 |
PaxDbi | Q9CPW4 |
PRIDEi | Q9CPW4 |
TopDownProteomicsi | Q9CPW4 |
Protocols and materials databases
Antibodypediai | 34448, 177 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000077755; ENSMUSP00000076933; ENSMUSG00000008475 |
GeneIDi | 67771 |
KEGGi | mmu:67771 |
UCSCi | uc007czj.2, mouse |
Organism-specific databases
CTDi | 10092 |
MGIi | MGI:1915021, Arpc5 |
Phylogenomic databases
eggNOGi | KOG3380, Eukaryota |
GeneTreei | ENSGT00940000154654 |
HOGENOMi | CLU_101888_1_1_1 |
InParanoidi | Q9CPW4 |
OMAi | GMALPGW |
OrthoDBi | 1565115at2759 |
PhylomeDBi | Q9CPW4 |
TreeFami | TF319716 |
Enzyme and pathway databases
Reactomei | R-MMU-2029482, Regulation of actin dynamics for phagocytic cup formation R-MMU-3928662, EPHB-mediated forward signaling R-MMU-5663213, RHO GTPases Activate WASPs and WAVEs R-MMU-6798695, Neutrophil degranulation R-MMU-8856828, Clathrin-mediated endocytosis |
Miscellaneous databases
BioGRID-ORCSi | 67771, 0 hits in 17 CRISPR screens |
ChiTaRSi | Arpc5, mouse |
PROi | PR:Q9CPW4 |
RNActi | Q9CPW4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000008475, Expressed in granulocyte and 290 other tissues |
Genevisiblei | Q9CPW4, MM |
Family and domain databases
Gene3Di | 1.25.40.190, 1 hit |
InterProi | View protein in InterPro IPR006789, ARPC5 IPR036743, ARPC5_sf |
PANTHERi | PTHR12644, PTHR12644, 1 hit |
Pfami | View protein in Pfam PF04699, P16-Arc, 1 hit |
PIRSFi | PIRSF039096, p16-ARC, 1 hit |
SUPFAMi | SSF69103, SSF69103, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARPC5_MOUSE | |
Accessioni | Q9CPW4Primary (citable) accession number: Q9CPW4 Secondary accession number(s): Q3U9T3, Q9D8E7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 12, 2005 |
Last sequence update: | January 23, 2007 | |
Last modified: | February 10, 2021 | |
This is version 142 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families