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UniProtKB - Q9CIV6 (DHAM_LACLA)
Protein
PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit DhaM
Gene
dhaM
Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Functioni
Component of the dihydroxyacetone kinase complex, which is responsible for the phosphoenolpyruvate (PEP)-dependent phosphorylation of dihydroxyacetone. DhaM serves as the phosphoryl donor. Is phosphorylated by phosphoenolpyruvate in an EI- and HPr-dependent reaction, and a phosphorelay system on histidine residues finally leads to phosphoryl transfer to DhaL and dihydroxyacetone.
1 PublicationMiscellaneous
Unlike the carbohydrate-specific transporters of the PTS, the complex DhaKML has no transport activity.1 Publication
Catalytic activityi
- EC:2.7.1.121By similarity
: glycerol degradation Pathwayi
This protein is involved in the pathway glycerol degradation, which is part of Polyol metabolism.CuratedView all proteins of this organism that are known to be involved in the pathway glycerol degradation and in Polyol metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 10 | Tele-phosphohistidine intermediate; for EIIA activityPROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- phosphoenolpyruvate-glycerone phosphotransferase activity Source: UniProtKB
GO - Biological processi
- glycerol catabolic process Source: UniProtKB-UniPathway
- phosphoenolpyruvate-dependent sugar phosphotransferase system Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Glycerol metabolism, Phosphotransferase system |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
UniPathwayi | UPA00616 |
Names & Taxonomyi
Protein namesi | Recommended name: PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit DhaM1 Publication (EC:2.7.1.121By similarity)Alternative name(s): PTS system EIIA component1 Publication Phosphotransferase enzyme IIA component1 Publication |
Gene namesi | Name:dhaM1 Publication Ordered Locus Names:LL0250 ORF Names:L47257 |
Organismi | Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) |
Taxonomic identifieri | 272623 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › |
Proteomesi |
|
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000270537 | 1 – 123 | PTS-dependent dihydroxyacetone kinase, phosphotransferase subunit DhaMAdd BLAST | 123 |
Proteomic databases
PaxDbi | Q9CIV6 |
Expressioni
Inductioni
Induced by dihydroxyacetone via the DhaQ-DhaS complex.1 Publication
Interactioni
Subunit structurei
Homodimer. The dihydroxyacetone kinase complex is composed of a homodimer of DhaM, a homodimer of DhaK and the subunit DhaL.
1 PublicationProtein-protein interaction databases
STRINGi | 272623.L47257 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9CIV6 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9CIV6 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 2 – 123 | PTS EIIA type-4PROSITE-ProRule annotation1 PublicationAdd BLAST | 122 |
Domaini
The EIIA type-4 domain is phosphorylated by phospho-HPr on a histidyl residue. Then, it transfers the phosphoryl group to the EIIB type-4 domain.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | COG3412, Bacteria |
HOGENOMi | CLU_045361_1_0_9 |
OMAi | KMNLEMV |
Family and domain databases
Gene3Di | 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit |
SUPFAMi | SSF53062, SSF53062, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9CIV6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTYGIVIVSH SPEIASGLKK LIREVAKNIS LTAIGGLENG EIGTSFDRVM
60 70 80 90 100
NAIEENEADN LLTFFDLGSA RMNLDLVSEM TDKELTIFNV PLIEGAYTAS
110 120
ALLEAGATFE AIKEQLEKML IEK
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005176 Genomic DNA Translation: AAK04348.1 |
PIRi | B86656 |
RefSeqi | NP_266406.1, NC_002662.1 WP_010905237.1, NC_002662.1 |
Genome annotation databases
EnsemblBacteriai | AAK04348; AAK04348; L47257 |
GeneIDi | 66441211 |
KEGGi | lla:L47257 |
PATRICi | fig|272623.7.peg.275 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005176 Genomic DNA Translation: AAK04348.1 |
PIRi | B86656 |
RefSeqi | NP_266406.1, NC_002662.1 WP_010905237.1, NC_002662.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3CR3 | X-ray | 2.10 | C/D | 3-123 | [»] | |
3CT6 | X-ray | 1.10 | A/B | 1-123 | [»] | |
SMRi | Q9CIV6 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 272623.L47257 |
Proteomic databases
PaxDbi | Q9CIV6 |
Genome annotation databases
EnsemblBacteriai | AAK04348; AAK04348; L47257 |
GeneIDi | 66441211 |
KEGGi | lla:L47257 |
PATRICi | fig|272623.7.peg.275 |
Phylogenomic databases
eggNOGi | COG3412, Bacteria |
HOGENOMi | CLU_045361_1_0_9 |
OMAi | KMNLEMV |
Enzyme and pathway databases
UniPathwayi | UPA00616 |
Miscellaneous databases
EvolutionaryTracei | Q9CIV6 |
Family and domain databases
Gene3Di | 3.40.50.510, 1 hit |
InterProi | View protein in InterPro IPR039643, DhaM IPR012844, DhaM_N IPR004701, PTS_EIIA_man-typ IPR036662, PTS_EIIA_man-typ_sf |
PANTHERi | PTHR38594, PTHR38594, 1 hit |
Pfami | View protein in Pfam PF03610, EIIA-man, 1 hit |
SUPFAMi | SSF53062, SSF53062, 1 hit |
TIGRFAMsi | TIGR02364, dha_pts, 1 hit |
PROSITEi | View protein in PROSITE PS51096, PTS_EIIA_TYPE_4, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | DHAM_LACLA | |
Accessioni | Q9CIV6Primary (citable) accession number: Q9CIV6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 9, 2007 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 99 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references