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UniProtKB - Q9CF42 (RBSK_LACLA)
Protein
Ribokinase
Gene
rbsK
Organism
Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis)
Status
Functioni
Catalyzes the phosphorylation of ribose at O-5 in a reaction requiring ATP and magnesium. The resulting D-ribose-5-phosphate can then be used either for sythesis of nucleotides, histidine, and tryptophan, or as a component of the pentose phosphate pathway.
UniRule annotationCatalytic activityi
- EC:2.7.1.15UniRule annotation
Cofactori
Mg2+UniRule annotationNote: Requires a divalent cation, most likely magnesium in vivo, as an electrophilic catalyst to aid phosphoryl group transfer. It is the chelate of the metal and the nucleotide that is the actual substrate.UniRule annotation
Activity regulationi
Activated by a monovalent cation that binds near, but not in, the active site. The most likely occupant of the site in vivo is potassium. Ion binding induces a conformational change that may alter substrate affinity.UniRule annotation
: D-ribose degradation Pathwayi
This protein is involved in step 2 of the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose.UniRule annotation This subpathway is part of the pathway D-ribose degradation, which is itself part of Carbohydrate metabolism.View all proteins of this organism that are known to be involved in the subpathway that synthesizes D-ribose 5-phosphate from beta-D-ribopyranose, the pathway D-ribose degradation and in Carbohydrate metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 139 | SubstrateUniRule annotation | 1 | |
Binding sitei | 183 | ATPUniRule annotation | 1 | |
Metal bindingi | 236 | PotassiumUniRule annotation | 1 | |
Metal bindingi | 238 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Active sitei | 242 | Proton acceptorUniRule annotation | 1 | |
Binding sitei | 242 | SubstrateUniRule annotation | 1 | |
Metal bindingi | 272 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 275 | Potassium; via carbonyl oxygenUniRule annotation | 1 | |
Metal bindingi | 277 | Potassium; via carbonyl oxygenUniRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 210 – 215 | ATPUniRule annotation | 6 | |
Nucleotide bindingi | 241 – 242 | ATPUniRule annotation | 2 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-UniRule
- metal ion binding Source: UniProtKB-KW
- ribokinase activity Source: UniProtKB-UniRule
GO - Biological processi
- D-ribose catabolic process Source: UniProtKB-UniRule
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Carbohydrate metabolism |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding, Potassium |
Enzyme and pathway databases
UniPathwayi | UPA00916;UER00889 |
Names & Taxonomyi
Protein namesi | Recommended name: RibokinaseUniRule annotation (EC:2.7.1.15UniRule annotation)Short name: RKUniRule annotation |
Gene namesi | Name:rbsKUniRule annotation Ordered Locus Names:LL1639 ORF Names:L86157 |
Organismi | Lactococcus lactis subsp. lactis (strain IL1403) (Streptococcus lactis) |
Taxonomic identifieri | 272623 [NCBI] |
Taxonomic lineagei | Bacteria › Firmicutes › Bacilli › Lactobacillales › Streptococcaceae › Lactococcus › |
Proteomesi |
|
Subcellular locationi
Cytoplasm and Cytosol
- Cytoplasm UniRule annotation
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080100 | 1 – 300 | RibokinaseAdd BLAST | 300 |
Proteomic databases
PaxDbi | Q9CF42 |
Interactioni
Subunit structurei
Homodimer.
UniRule annotationProtein-protein interaction databases
STRINGi | 272623.L86157 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 11 – 13 | Substrate bindingUniRule annotation | 3 | |
Regioni | 39 – 43 | Substrate bindingUniRule annotation | 5 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | COG0524, Bacteria |
HOGENOMi | CLU_027634_2_2_9 |
OMAi | DIVLIQQ |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR02152, D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9CF42-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MTKVSVIGSI SMDLVTRTNR VPNAGETVFG EDFAMVPGGK GANQAVAFAR
60 70 80 90 100
LSPNEVSMIG AVGKDAFGES ILQNFKENAV LFENVGTVPQ TTGIAQITLY
110 120 130 140 150
DDDNRIIIIP GANNEVLPSY LADLWEKIKE SQLVILQNEI PHETNLAIAK
160 170 180 190 200
FCKENAIKVL YNPAPARKTD LEMIDFVDYI TPNEHECKEL FPNLALEEIL
210 220 230 240 250
KKYSNRLIVT LGSEGVIFHD GETLQKIPAI KAKVVDTTGA GDTFNGAFAF
260 270 280 290 300
GLTENLSISD SIRLAVVASH LSIQKFGAQG GMPKLSEVKA KLKELEINLY
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005176 Genomic DNA Translation: AAK05737.1 |
PIRi | G86829 |
RefSeqi | NP_267795.1, NC_002662.1 WP_010906072.1, NC_002662.1 |
Genome annotation databases
EnsemblBacteriai | AAK05737; AAK05737; L86157 |
GeneIDi | 60355721 |
KEGGi | lla:L86157 |
PATRICi | fig|272623.7.peg.1761 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AE005176 Genomic DNA Translation: AAK05737.1 |
PIRi | G86829 |
RefSeqi | NP_267795.1, NC_002662.1 WP_010906072.1, NC_002662.1 |
3D structure databases
SMRi | Q9CF42 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 272623.L86157 |
Proteomic databases
PaxDbi | Q9CF42 |
Genome annotation databases
EnsemblBacteriai | AAK05737; AAK05737; L86157 |
GeneIDi | 60355721 |
KEGGi | lla:L86157 |
PATRICi | fig|272623.7.peg.1761 |
Phylogenomic databases
eggNOGi | COG0524, Bacteria |
HOGENOMi | CLU_027634_2_2_9 |
OMAi | DIVLIQQ |
Enzyme and pathway databases
UniPathwayi | UPA00916;UER00889 |
Family and domain databases
CDDi | cd01174, ribokinase, 1 hit |
Gene3Di | 3.40.1190.20, 1 hit |
HAMAPi | MF_01987, Ribokinase, 1 hit |
InterProi | View protein in InterPro IPR002173, Carboh/pur_kinase_PfkB_CS IPR011877, D_ribokin IPR011611, PfkB_dom IPR002139, Ribo/fructo_kinase IPR029056, Ribokinase-like |
Pfami | View protein in Pfam PF00294, PfkB, 1 hit |
PRINTSi | PR00990, RIBOKINASE |
SUPFAMi | SSF53613, SSF53613, 1 hit |
TIGRFAMsi | TIGR02152, D_ribokin_bact, 1 hit |
PROSITEi | View protein in PROSITE PS00584, PFKB_KINASES_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | RBSK_LACLA | |
Accessioni | Q9CF42Primary (citable) accession number: Q9CF42 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 109 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Prokaryotic Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families