Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

4-diphosphocytidyl-2-C-methyl-D-erythritol kinase

Gene

ispE

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol.UniRule annotation

Catalytic activityi

ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 3 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase (ispF)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (flavodoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei13UniRule annotation1
Active sitei140UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi98 – 108ATPUniRule annotationAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase
Biological processIsoprene biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-274-MONOMER
UniPathwayi
UPA00056;UER00094

Names & Taxonomyi

Protein namesi
Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinaseUniRule annotation (EC:2.7.1.148UniRule annotation)
Short name:
CMKUniRule annotation
Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinaseUniRule annotation
Gene namesi
Name:ispEUniRule annotation
Ordered Locus Names:ML0242
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0242

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001892331 – 3114-diphosphocytidyl-2-C-methyl-D-erythritol kinaseAdd BLAST311

Interactioni

Protein-protein interaction databases

STRINGi272631.ML0242

Structurei

3D structure databases

ProteinModelPortaliQ9CD51
SMRiQ9CD51
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the GHMP kinase family. IspE subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CTR Bacteria
COG1947 LUCA
HOGENOMiHOG000019600
KOiK00919
OMAiVACDALW
OrthoDBiPOG091H00XD

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF010376 IspE, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CD51-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MPTGSVTVQV PGKINLYLAV GDCCDNGYHE LVTVFHAVSL VDQVTVRNAD
60 70 80 90 100
VLSLGLVGEG ANHVPTDEHN IAWRAAELMA EHVGRAPDVS IMIDKSIPVA
110 120 130 140 150
GGMAGGSADA AAVLVAMNSL WELSLPRRDL CMLAAKLGSD VPFALHGGTA
160 170 180 190 200
LGTGRGEELA TVLSRATFHW VLAFADSSLL TPAVYTEFDR LRDVGNPPRL
210 220 230 240 250
AEPGPVLAAL VAADPEQLAP LLGNELQAAA VSLDPALRCA LRAGMEAGAL
260 270 280 290 300
AGIVSGSGPT CAFLCASATS AIDVGAQLAG AGVCRTVRVA TGPVPGARVV
310
HAPMSRGLND M
Length:311
Mass (Da):31,794
Last modified:June 1, 2001 - v1
Checksum:iDF9F572BACD81C22
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583917 Genomic DNA Translation: CAC29750.1
PIRiB86939
RefSeqiNP_301301.1, NC_002677.1
WP_010907625.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC29750; CAC29750; CAC29750
GeneIDi908715
KEGGimle:ML0242
PATRICifig|272631.5.peg.376

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AL583917 Genomic DNA Translation: CAC29750.1
PIRiB86939
RefSeqiNP_301301.1, NC_002677.1
WP_010907625.1, NC_002677.1

3D structure databases

ProteinModelPortaliQ9CD51
SMRiQ9CD51
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi272631.ML0242

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAC29750; CAC29750; CAC29750
GeneIDi908715
KEGGimle:ML0242
PATRICifig|272631.5.peg.376

Organism-specific databases

LepromaiML0242

Phylogenomic databases

eggNOGiENOG4105CTR Bacteria
COG1947 LUCA
HOGENOMiHOG000019600
KOiK00919
OMAiVACDALW
OrthoDBiPOG091H00XD

Enzyme and pathway databases

UniPathwayi
UPA00056;UER00094

BioCyciMLEP272631:G1GT5-274-MONOMER

Family and domain databases

Gene3Di3.30.230.10, 1 hit
3.30.70.890, 1 hit
HAMAPiMF_00061 IspE, 1 hit
InterProiView protein in InterPro
IPR013750 GHMP_kinase_C_dom
IPR036554 GHMP_kinase_C_sf
IPR006204 GHMP_kinase_N_dom
IPR004424 IspE
IPR020568 Ribosomal_S5_D2-typ_fold
IPR014721 Ribosomal_S5_D2-typ_fold_subgr
PfamiView protein in Pfam
PF08544 GHMP_kinases_C, 1 hit
PF00288 GHMP_kinases_N, 1 hit
PIRSFiPIRSF010376 IspE, 1 hit
SUPFAMiSSF54211 SSF54211, 1 hit
SSF55060 SSF55060, 1 hit
TIGRFAMsiTIGR00154 ispE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiISPE_MYCLE
AccessioniPrimary (citable) accession number: Q9CD51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: June 1, 2001
Last modified: October 10, 2018
This is version 89 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again