Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Pyridoxal 5'-phosphate synthase subunit PdxT

Gene

pdxT

Organism
Mycobacterium leprae (strain TN)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the hydrolysis of glutamine to glutamate and ammonia as part of the biosynthesis of pyridoxal 5'-phosphate. The resulting ammonia molecule is channeled to the active site of PdxS.UniRule annotation

Catalytic activityi

D-ribose 5-phosphate + D-glyceraldehyde 3-phosphate + L-glutamine = pyridoxal 5'-phosphate + L-glutamate + 3 H2O + phosphate.UniRule annotation
L-glutamine + H2O = L-glutamate + NH3.UniRule annotation

Pathwayi: pyridoxal 5'-phosphate biosynthesis

This protein is involved in the pathway pyridoxal 5'-phosphate biosynthesis, which is part of Cofactor biosynthesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway pyridoxal 5'-phosphate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei81NucleophileUniRule annotation1
Binding sitei113L-glutamineUniRule annotation1
Active sitei177Charge relay systemUniRule annotation1
Active sitei179Charge relay systemUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Lyase
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMLEP272631:G1GT5-525-MONOMER
UniPathwayiUPA00245

Protein family/group databases

MEROPSiC26.A32

Names & Taxonomyi

Protein namesi
Recommended name:
Pyridoxal 5'-phosphate synthase subunit PdxTUniRule annotation (EC:4.3.3.6UniRule annotation)
Alternative name(s):
Pdx2UniRule annotation
Pyridoxal 5'-phosphate synthase glutaminase subunitUniRule annotation (EC:3.5.1.2UniRule annotation)
Gene namesi
Name:pdxTUniRule annotation
Ordered Locus Names:ML0474
OrganismiMycobacterium leprae (strain TN)
Taxonomic identifieri272631 [NCBI]
Taxonomic lineageiBacteriaActinobacteriaCorynebacterialesMycobacteriaceaeMycobacterium
Proteomesi
  • UP000000806 Componenti: Chromosome

Organism-specific databases

LepromaiML0474

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001356471 – 198Pyridoxal 5'-phosphate synthase subunit PdxTAdd BLAST198

Interactioni

Subunit structurei

In the presence of PdxS, forms a dodecamer of heterodimers. Only shows activity in the heterodimer.UniRule annotation

Protein-protein interaction databases

STRINGi272631.ML0474

Structurei

3D structure databases

ProteinModelPortaliQ9CCT5
SMRiQ9CCT5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni49 – 51L-glutamine bindingUniRule annotation3
Regioni141 – 142L-glutamine bindingUniRule annotation2

Sequence similaritiesi

Belongs to the glutaminase PdxT/SNO family.UniRule annotation

Keywords - Domaini

Glutamine amidotransferase

Phylogenomic databases

eggNOGiENOG4108UHX Bacteria
COG0311 LUCA
HOGENOMiHOG000039949
KOiK08681
OrthoDBiPOG091H084H

Family and domain databases

CDDicd01749 GATase1_PB, 1 hit
Gene3Di3.40.50.880, 1 hit
HAMAPiMF_01615 PdxT, 1 hit
InterProiView protein in InterPro
IPR029062 Class_I_gatase-like
IPR002161 PdxT/SNO
IPR021196 PdxT/SNO_CS
PANTHERiPTHR31559 PTHR31559, 1 hit
PfamiView protein in Pfam
PF01174 SNO, 1 hit
PIRSFiPIRSF005639 Glut_amidoT_SNO, 1 hit
SUPFAMiSSF52317 SSF52317, 1 hit
TIGRFAMsiTIGR03800 PLP_synth_Pdx2, 1 hit
PROSITEiView protein in PROSITE
PS01236 PDXT_SNO_1, 1 hit
PS51130 PDXT_SNO_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q9CCT5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFPRVGVLA LQGDTREHLT ALREAGADSM PVRRRGELDE VDALVIPGGE
60 70 80 90 100
STTISHLLLD CELLEPLRAR LADGLPAYGA CTGMILLASE ILDAGVCGRE
110 120 130 140 150
ALPLGAIDIT VRRNAFGRQV DSFEGDIGFA GLVDPVRAVF IRAPWVERAG
160 170 180 190
DGVQVLAQAA GHAVAVRQGS MLATAFHPEM TSDRRIHQLF VDIVNGIA
Length:198
Mass (Da):21,075
Last modified:July 5, 2005 - v2
Checksum:iECF84E45826802CD
GO

Sequence cautioni

The sequence AAA17085 differs from that shown. Reason: Erroneous initiation.Curated
The sequence CAC29982 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00011 Genomic DNA Translation: AAA17085.1 Different initiation.
AL583918 Genomic DNA Translation: CAC29982.1 Different initiation.
PIRiB86968
S72721
RefSeqiNP_301419.2, NC_002677.1
WP_010907743.1, NC_002677.1

Genome annotation databases

EnsemblBacteriaiCAC29982; CAC29982; CAC29982
GeneIDi909221
KEGGimle:ML0474
PATRICifig|272631.5.peg.835

Similar proteinsi

Entry informationi

Entry nameiPDXT_MYCLE
AccessioniPrimary (citable) accession number: Q9CCT5
Secondary accession number(s): Q49637
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 5, 2005
Last sequence update: July 5, 2005
Last modified: February 28, 2018
This is version 80 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health