UniProtKB - Q9CB01 (RABF1_ARATH)
Protein
Ras-related protein RABF1
Gene
RABF1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Endosomal protein probably involved in endocytosis. Probably not involved in vacuolar trafficking.2 Publications
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 40 – 47 | GTPBy similarity | 8 | |
Nucleotide bindingi | 89 – 93 | GTPBy similarity | 5 | |
Nucleotide bindingi | 147 – 150 | GTPBy similarity | 4 | |
Nucleotide bindingi | 177 – 178 | GTPBy similarity | 2 |
GO - Molecular functioni
- GTPase activity Source: TAIR
- GTP binding Source: UniProtKB-KW
GO - Biological processi
- early endosome to late endosome transport Source: TAIR
- endocytosis Source: UniProtKB-KW
- intracellular protein transport Source: GO_Central
Keywordsi
Biological process | Endocytosis |
Ligand | GTP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT3G54840-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Ras-related protein RABF11 PublicationShort name: AtRABF11 Publication Alternative name(s): Ras-related protein Ara-61 Publication Ras-related protein Rab5C1 Publication Short name: AtRab5C1 Publication |
Gene namesi | Name:RABF11 Publication Synonyms:ARA-61 Publication, RAB5C1 Publication Ordered Locus Names:At3g54840Imported ORF Names:F28P10.180Imported |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT3G54840 |
TAIRi | locus:2082672, AT3G54840 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Plasma membrane
- Cell membrane 1 Publication; Lipid-anchor 1 Publication
Endosome
- Early endosome membrane 1 Publication
- Endosome membrane 1 Publication
Cytosol
- cytosol Source: TAIR
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Endosome
- cytoplasmic side of endosome membrane Source: TAIR
- early endosome Source: TAIR
- early endosome membrane Source: UniProtKB-SubCell
- endosome Source: TAIR
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- endomembrane system Source: GO_Central
Keywords - Cellular componenti
Cell membrane, Endoplasmic reticulum, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 2 | G → A: Loss of myristoylation. Loss of targeting to membrane. 1 Publication | 1 | |
Mutagenesisi | 3 | C → S: Loss of palmitoylation. 1 Publication | 1 | |
Mutagenesisi | 47 | S → N: Dominant negative (GDP-bound form); no effect on vacuolar trafficking. No effect on the interaction with VPS9A. 2 Publications | 1 | |
Mutagenesisi | 93 | Q → L: Constitutively active (GTP-bound form); no effect on targeting to plasma membrane. Loss of interaction with VPS9A. 2 Publications | 1 | |
Mutagenesisi | 147 | N → I: Blocks nucleotide binding; loss of targeting to endosomal membrane. No effect on the interaction with VPS9A. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed | |||
ChainiPRO_0000406606 | 2 – 202 | Ras-related protein RABF1Add BLAST | 201 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Lipidationi | 2 | N-myristoyl glycine1 Publication | 1 | |
Lipidationi | 3 | S-palmitoyl cysteine1 Publication | 1 |
Post-translational modificationi
Although this sequence lacks the C-terminal cysteine motifs subject to isoprenylation in other Rab proteins, it does have N-terminal N-myristoylation and S-palmitoylation.2 Publications
Keywords - PTMi
Lipoprotein, Myristate, PalmitateProteomic databases
PaxDbi | Q9CB01 |
PRIDEi | Q9CB01 |
ProteomicsDBi | 236764 [Q9CB01-1] |
PTM databases
iPTMneti | Q9CB01 |
SwissPalmi | Q9CB01 |
Expressioni
Tissue specificityi
Widely expressed.1 Publication
Inductioni
Activated by VPS9A.1 Publication
Gene expression databases
ExpressionAtlasi | Q9CB01, baseline and differential |
Genevisiblei | Q9CB01, AT |
Interactioni
Subunit structurei
Protein-protein interaction databases
BioGRIDi | 9965, 4 interactors |
IntActi | Q9CB01, 6 interactors |
STRINGi | 3702.AT3G54840.1 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 62 – 70 | Effector regionBy similarity | 9 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | Q9CB01 |
PhylomeDBi | Q9CB01 |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9CB01-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MGCASSLPDR NSGTLSGLSN SENAVPADAK NLRVKLVLLG DSGVGKSCIV
60 70 80 90 100
LRFVRGQFDA TSKVTVGASF LSQTIALQDS TTVKFEIWDT AGQERYSALA
110 120 130 140 150
PLYYRGAGVA VIVYDITSPE SFKKAQYWVK ELQKHGSPDI VMALVGNKAD
160 170 180 190 200
LHEKREVPTE DGMELAEKNG MFFIETSAKT ADNINQLFEE IGKRLPRPAP
SS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A1I9LT70 | A0A1I9LT70_ARATH | Ras-related small GTP-binding famil... | ARA6 ARA-6, AtARA6, ATRAB5C, ATRABF1, RABF1 | 168 | Annotation score: |
Sequence cautioni
The sequence CAB41100 differs from that shown. Reason: Erroneous gene model prediction.Curated
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_040836 | 190 – 202 | EIGKR…PAPSS → CTHV in isoform 2. 1 PublicationAdd BLAST | 13 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB007766 mRNA Translation: BAB32953.1 BT002860 mRNA Translation: AAO22677.1 AL049655 Genomic DNA Translation: CAB41100.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79298.1 CP002686 Genomic DNA Translation: AEE79299.1 BT004392 mRNA Translation: AAO42386.1 AK316975 mRNA Translation: BAH19672.1 |
PIRi | T06736 |
RefSeqi | NP_001078287.1, NM_001084818.1 [Q9CB01-2] NP_567008.1, NM_115341.3 [Q9CB01-1] |
Genome annotation databases
EnsemblPlantsi | AT3G54840.1; AT3G54840.1; AT3G54840 [Q9CB01-1] AT3G54840.2; AT3G54840.2; AT3G54840 [Q9CB01-2] |
GeneIDi | 824649 |
Gramenei | AT3G54840.1; AT3G54840.1; AT3G54840 [Q9CB01-1] AT3G54840.2; AT3G54840.2; AT3G54840 [Q9CB01-2] |
KEGGi | ath:AT3G54840 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB007766 mRNA Translation: BAB32953.1 BT002860 mRNA Translation: AAO22677.1 AL049655 Genomic DNA Translation: CAB41100.1 Sequence problems. CP002686 Genomic DNA Translation: AEE79298.1 CP002686 Genomic DNA Translation: AEE79299.1 BT004392 mRNA Translation: AAO42386.1 AK316975 mRNA Translation: BAH19672.1 |
PIRi | T06736 |
RefSeqi | NP_001078287.1, NM_001084818.1 [Q9CB01-2] NP_567008.1, NM_115341.3 [Q9CB01-1] |
3D structure databases
SMRi | Q9CB01 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 9965, 4 interactors |
IntActi | Q9CB01, 6 interactors |
STRINGi | 3702.AT3G54840.1 |
PTM databases
iPTMneti | Q9CB01 |
SwissPalmi | Q9CB01 |
Proteomic databases
PaxDbi | Q9CB01 |
PRIDEi | Q9CB01 |
ProteomicsDBi | 236764 [Q9CB01-1] |
Genome annotation databases
EnsemblPlantsi | AT3G54840.1; AT3G54840.1; AT3G54840 [Q9CB01-1] AT3G54840.2; AT3G54840.2; AT3G54840 [Q9CB01-2] |
GeneIDi | 824649 |
Gramenei | AT3G54840.1; AT3G54840.1; AT3G54840 [Q9CB01-1] AT3G54840.2; AT3G54840.2; AT3G54840 [Q9CB01-2] |
KEGGi | ath:AT3G54840 |
Organism-specific databases
Araporti | AT3G54840 |
TAIRi | locus:2082672, AT3G54840 |
Phylogenomic databases
eggNOGi | KOG0092, Eukaryota |
HOGENOMi | CLU_041217_10_2_1 |
InParanoidi | Q9CB01 |
PhylomeDBi | Q9CB01 |
Enzyme and pathway databases
BioCyci | ARA:AT3G54840-MONOMER |
Miscellaneous databases
PROi | PR:Q9CB01 |
Gene expression databases
ExpressionAtlasi | Q9CB01, baseline and differential |
Genevisiblei | Q9CB01, AT |
Family and domain databases
InterProi | View protein in InterPro IPR027417, P-loop_NTPase IPR005225, Small_GTP-bd_dom IPR001806, Small_GTPase |
Pfami | View protein in Pfam PF00071, Ras, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
TIGRFAMsi | TIGR00231, small_GTP, 1 hit |
PROSITEi | View protein in PROSITE PS51419, RAB, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RABF1_ARATH | |
Accessioni | Q9CB01Primary (citable) accession number: Q9CB01 Secondary accession number(s): A8MRL2, Q9SV33 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 5, 2011 |
Last sequence update: | June 1, 2001 | |
Last modified: | October 7, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families