ADO3

Functionⁱ

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. Forms a complex with 'GIGANTEA' (GI) to regulate 'CONSTANS' (CO) expression. Promotes CO expression during the light period of long days by decreasing the stability of CDF1 and CDF2 and by interacting directly with the CO protein and stabilizing it. ADO3 function is mainly GI dependent. Does not act as a regulator of CDF1 transcription. The interactions of ADO1/ZTL and ADO2 with ADO3 prevent its interaction with CDF1.8 Publications

Miscellaneous

'Adagio' means slowly in Italian.

Pathwayⁱ: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functionⁱ

photoreceptor activity Source: UniProtKB-KW

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

circadian rhythm
Source: TAIR
Inferred from direct assayⁱ
- 17872410
flower development Source: UniProtKB-KW
positive regulation of flower development
Source: TAIR
Inferred from mutant phenotypeⁱ
- 10847687
protein-chromophore linkage Source: UniProtKB-KW
protein ubiquitination Source: UniProtKB-UniPathway
regulation of gene expression
Source: TAIR
Inferred from mutant phenotypeⁱ
- 22628657
regulation of transcription, DNA-templated
Source: TAIR
Inferred from direct assayⁱ
- 17872410
response to blue light
Source: TAIR
Inferred from direct assayⁱ
- 17872410

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Photoreceptor protein, Receptor
Biological process	Biological rhythms, Flowering, Sensory transduction, Ubl conjugation pathway
Ligand	Chromophore, Flavoprotein, FMN

Enzyme and pathway databases

UniPathwayⁱ	UPA00143

UniPathwayⁱ

UPA00143

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Adagio protein 3 Alternative name(s): F-box only protein 2a Short name: FBX2a Flavin-binding kelch repeat F-box protein 1
Gene namesⁱ	Name:ADO3 Synonyms:FKF1 Ordered Locus Names:At1g68050 ORF Names:T23K23.10
Organismⁱ	Arabidopsis thaliana (Mouse-ear cress)
Taxonomic identifierⁱ	3702 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › Mesangiospermae › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis
Proteomesⁱ	UP000006548 Componentⁱ: Chromosome 1

Organism-specific databases

Arabidopsis Information Portal More...Araportⁱ	AT1G68050
TAIRⁱ	locus:2200176 AT1G68050

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

Pathology & Biotechⁱ

Disruption phenotypeⁱ

Late flowering.1 Publication

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	91	C → A: No FMN binding and decreased interaction with GI. 2 Publications	1
Mutagenesisⁱ	92	R → D: Decreased interaction with GI. 1 Publication	1
Mutagenesisⁱ	163	Q → L: Decreased interaction with GI. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000119958	1 – 619	Adagio protein 3Add BLAST		619

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Modified residueⁱ	91	S-4a-FMN cysteine		1

Post-translational modificationⁱ

FMN binds covalently to cysteine after exposure to blue light and is reversed in the dark.

Proteomic databases

PaxDbⁱ	Q9C9W9
PRIDEⁱ	Q9C9W9

PTM databases

iPTMnetⁱ	Q9C9W9

iPTMnetⁱ

Q9C9W9

Expressionⁱ

Tissue specificityⁱ

Highly expressed in stomata and leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments.2 Publications

Inductionⁱ

Circadian-regulation. Expression is higher during the late light phase than during the dark phase.3 Publications

Gene expression databases

ExpressionAtlasⁱ	Q9C9W9 baseline and differential
Genevisibleⁱ	Q9C9W9 AT

Interactionⁱ

Subunit structureⁱ

Interacts with ADO1 (via Kelch repeats), ADO2 (via Kelch repeats), SKP1A/ASK1, SKP1B/ASK2, ASK3, SKP1K/ASK11, ASK12, ASK13 and SKP1N/ASK14. Interacts (via Kelch repeats) with CDF1, CDF2 and CDF3. Interacts (via N-terminus) with CO and GI (via N-terminus) in a blue-light-dependent manner.7 Publications

Binary interactionsⁱ

With	Entry	#Exp.	IntAct
CDF1	Q8W1E3	2	EBI-401228,EBI-1536051
CDF2	Q93ZL5	2	EBI-401228,EBI-1536103
CDF3	Q8LFV3	2	EBI-401228,EBI-1536119
GI	Q9SQI2	11	EBI-401228,EBI-446380
SKP1B	Q9FHW7	5	EBI-401228,EBI-604076
SRK2G	P43292	3	EBI-401228,EBI-401174

Protein-protein interaction databases

BioGridⁱ	28354, 24 interactors
Database of interacting proteins More...DIPⁱ	DIP-31333N
IntActⁱ	Q9C9W9, 16 interactors
STRINGⁱ	3702.AT1G68050.1

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9C9W9
SMRⁱ	Q9C9W9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	44 – 123	PASPROSITE-ProRule annotationAdd BLAST	80
Domainⁱ	127 – 168	PACAdd BLAST	42
Domainⁱ	211 – 257	F-boxAdd BLAST	47
Repeatⁱ	304 – 354	Kelch 1Add BLAST	51
Repeatⁱ	357 – 404	Kelch 2Add BLAST	48
Repeatⁱ	409 – 457	Kelch 3Add BLAST	49
Repeatⁱ	462 – 513	Kelch 4Add BLAST	52
Repeatⁱ	523 – 571	Kelch 5Add BLAST	49

Sequence similaritiesⁱ

Belongs to the ADAGIO family.Curated

Keywords - Domainⁱ

Kelch repeat, Repeat

Phylogenomic databases

eggNOGⁱ	ENOG410IKJW Eukaryota ENOG410XRGE LUCA
HOGENOMⁱ	HOG000238789
InParanoidⁱ	Q9C9W9
KEGG Orthology (KO) More...KOⁱ	K12116
OMAⁱ	AYTMDAG
Database of Orthologous Groups More...OrthoDBⁱ	EOG093604YP
PhylomeDBⁱ	Q9C9W9

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00130 PAS, 1 hit
Gene3Dⁱ	2.120.10.80, 2 hits
InterProⁱ	View protein in InterPro IPR036047 F-box-like_dom_sf IPR015915 Kelch-typ_b-propeller IPR011498 Kelch_2 IPR001610 PAC IPR000014 PAS IPR035965 PAS-like_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF07646 Kelch_2, 1 hit PF13426 PAS_9, 1 hit
SMARTⁱ	View protein in SMART SM00086 PAC, 1 hit
SUPFAMⁱ	SSF117281 SSF117281, 2 hits SSF55785 SSF55785, 1 hit SSF81383 SSF81383, 1 hit
TIGRFAMsⁱ	TIGR00229 sensory_box, 1 hit
PROSITEⁱ	View protein in PROSITE PS50112 PAS, 1 hit

Sequenceⁱ

Sequence statusⁱ: Complete.

Q9C9W9-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MAREHAIGEA TGKRKKRGRV EEAEEYCNDG IEEQVEDEKL PLEVGMFYYP 
        60         70         80         90        100
MTPPSFIVSD ALEPDFPLIY VNRVFEVFTG YRADEVLGRN CRFLQYRDPR 
       110        120        130        140        150
AQRRHPLVDP VVVSEIRRCL EEGIEFQGEL LNFRKDGTPL VNRLRLAPIR 
       160        170        180        190        200
DDDGTITHVI GIQVFSETTI DLDRVSYPVF KHKQQLDQTS ECLFPSGSPR 
       210        220        230        240        250
FKEHHEDFCG ILQLSDEVLA HNILSRLTPR DVASIGSACR RLRQLTKNES 
       260        270        280        290        300
VRKMVCQNAW GKEITGTLEI MTKKLRWGRL ARELTTLEAV CWRKFTVGGI 
       310        320        330        340        350
VQPSRCNFSA CAVGNRLVLF GGEGVNMQPL DDTFVLNLDA ECPEWQRVRV 
       360        370        380        390        400
TSSPPGRWGH TLSCLNGSWL VVFGGCGRQG LLNDVFVLDL DAKHPTWKEV 
       410        420        430        440        450
AGGTPPLPRS WHSSCTIEGS KLVVSGGCTD AGVLLSDTFL LDLTTDKPTW 
       460        470        480        490        500
KEIPTSWAPP SRLGHSLSVF GRTKILMFGG LANSGHLKLR SGEAYTIDLE 
       510        520        530        540        550
DEEPRWRELE CSAFPGVVVP PPRLDHVAVS MPCGRVIIFG GSIAGLHSPS 
       560        570        580        590        600
QLFLIDPAEE KPSWRILNVP GKPPKLAWGH STCVVGGTRV LVLGGHTGEE 
       610 
WILNELHELC LASRQDSDL

Length:619

Mass (Da):69,063

Last modified:June 1, 2001 - v1

Checksum:ⁱAC90C0641149A3D8

GO

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	484	S → I in AAF32298 (PubMed:10847687).Curated	1
Sequence conflictⁱ	484	S → I in AAK27435 (PubMed:11260718).Curated	1
Sequence conflictⁱ	581	S → N in AAF32298 (PubMed:10847687).Curated	1
Sequence conflictⁱ	581	S → N in AAK27435 (PubMed:11260718).Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF216523 mRNA Translation: AAF32298.2 AF252296 mRNA Translation: AAK27435.1 AC012563 Genomic DNA Translation: AAG51994.1 CP002684 Genomic DNA Translation: AEE34741.1 AY064999 mRNA Translation: AAL57647.1 AY113029 mRNA Translation: AAM47337.1
PIRⁱ	F96703
NCBI Reference Sequences More...RefSeqⁱ	NP_564919.1, NM_105475.4
UniGeneⁱ	At.20568

Genome annotation databases

EnsemblPlantsⁱ	AT1G68050.1; AT1G68050.1; AT1G68050
GeneIDⁱ	843133
Grameneⁱ	AT1G68050.1; AT1G68050.1; AT1G68050
KEGGⁱ	ath:AT1G68050

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9C9W9	FKF1		ARATH		619	UniRef100_Q9C9W9

Protein	Similar proteins		Species	Score	Length	Source
Q9C9W9	FKF1		ARATH		619	UniRef90_Q9C9W9
Uncharacterized protein		Capsella rubella		624
Flavin-binding kelch domain F box protein		ARALP		623
Flavin-binding kelch domain F box protein		ARALL		623
Uncharacterized protein		EUTSA		623
+50

Protein	Similar proteins		Species	Score	Length	Source
Q9C9W9	Adagio protein 1	)	ARATH		609	UniRef50_Q94BT6
Putative adagio-like protein 2		ORYSJ		635
F-box protein ZEITLUPE (Fragment)		BRARP		157
Putative LOV domain-containing protein		Draba oligosperma		612
Putative LOV domain-containing protein		ARAAL		609
+1306

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF216523 mRNA Translation: AAF32298.2 AF252296 mRNA Translation: AAK27435.1 AC012563 Genomic DNA Translation: AAG51994.1 CP002684 Genomic DNA Translation: AEE34741.1 AY064999 mRNA Translation: AAL57647.1 AY113029 mRNA Translation: AAM47337.1
PIRⁱ	F96703
RefSeqⁱ	NP_564919.1, NM_105475.4
UniGeneⁱ	At.20568

3D structure databases

ProteinModelPortalⁱ	Q9C9W9
SMRⁱ	Q9C9W9
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	28354, 24 interactors
DIPⁱ	DIP-31333N
IntActⁱ	Q9C9W9, 16 interactors
STRINGⁱ	3702.AT1G68050.1

PTM databases

iPTMnetⁱ	Q9C9W9

iPTMnetⁱ

Q9C9W9

Proteomic databases

PaxDbⁱ	Q9C9W9
PRIDEⁱ	Q9C9W9

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

EnsemblPlantsⁱ	AT1G68050.1; AT1G68050.1; AT1G68050
GeneIDⁱ	843133
Grameneⁱ	AT1G68050.1; AT1G68050.1; AT1G68050
KEGGⁱ	ath:AT1G68050

Organism-specific databases

Araportⁱ	AT1G68050
TAIRⁱ	locus:2200176 AT1G68050

Phylogenomic databases

eggNOGⁱ	ENOG410IKJW Eukaryota ENOG410XRGE LUCA
HOGENOMⁱ	HOG000238789
InParanoidⁱ	Q9C9W9
KOⁱ	K12116
OMAⁱ	AYTMDAG
OrthoDBⁱ	EOG093604YP
PhylomeDBⁱ	Q9C9W9

Enzyme and pathway databases

UniPathwayⁱ	UPA00143

UniPathwayⁱ

UPA00143

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9C9W9

PROⁱ

PR:Q9C9W9

Gene expression databases

ExpressionAtlasⁱ	Q9C9W9 baseline and differential
Genevisibleⁱ	Q9C9W9 AT

Family and domain databases

CDDⁱ	cd00130 PAS, 1 hit
Gene3Dⁱ	2.120.10.80, 2 hits
InterProⁱ	View protein in InterPro IPR036047 F-box-like_dom_sf IPR015915 Kelch-typ_b-propeller IPR011498 Kelch_2 IPR001610 PAC IPR000014 PAS IPR035965 PAS-like_dom_sf
Pfamⁱ	View protein in Pfam PF07646 Kelch_2, 1 hit PF13426 PAS_9, 1 hit
SMARTⁱ	View protein in SMART SM00086 PAC, 1 hit
SUPFAMⁱ	SSF117281 SSF117281, 2 hits SSF55785 SSF55785, 1 hit SSF81383 SSF81383, 1 hit
TIGRFAMsⁱ	TIGR00229 sensory_box, 1 hit
PROSITEⁱ	View protein in PROSITE PS50112 PAS, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	ADO3_ARATH
Accessionⁱ	Q9C9W9Primary (citable) accession number: Q9C9W9 Secondary accession number(s): Q9M648
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 8, 2005
	Last sequence update:	June 1, 2001
	Last modified:	May 23, 2018
	This is version 130 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Plant Protein Annotation Program

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UniProtKB - Q9C9W9 (ADO3_ARATH)

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Adagio protein 3

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Miscellaneous

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: protein ubiquitination

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: protein ubiquitination

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ