UniProtKB - Q9C9W9 (ADO3_ARATH)
Adagio protein 3
ADO3
Functioni
Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. Forms a complex with 'GIGANTEA' (GI) to regulate 'CONSTANS' (CO) expression. Promotes CO expression during the light period of long days by decreasing the stability of CDF1 and CDF2 and by interacting directly with the CO protein and stabilizing it. ADO3 function is mainly GI dependent. Does not act as a regulator of CDF1 transcription. The interactions of ADO1/ZTL and ADO2 with ADO3 prevent its interaction with CDF1.
8 PublicationsMiscellaneous
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- photoreceptor activity Source: UniProtKB-KW
GO - Biological processi
- circadian rhythm Source: TAIR
- flower development Source: UniProtKB-KW
- negative regulation of cellulose biosynthetic process Source: TAIR
- positive regulation of flower development Source: TAIR
- protein-chromophore linkage Source: UniProtKB-KW
- protein ubiquitination Source: UniProtKB-UniPathway
- regulation of gene expression Source: TAIR
- regulation of transcription, DNA-templated Source: TAIR
- response to blue light Source: TAIR
Keywordsi
Molecular function | Photoreceptor protein, Receptor |
Biological process | Biological rhythms, Flowering, Sensory transduction, Ubl conjugation pathway |
Ligand | Chromophore, Flavoprotein, FMN |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Adagio protein 3Alternative name(s): F-box only protein 2a Short name: FBX2a Flavin-binding kelch repeat F-box protein 1 |
Gene namesi | Name:ADO3 Synonyms:FKF1 Ordered Locus Names:At1g68050 ORF Names:T23K23.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G68050 |
TAIRi | locus:2200176, AT1G68050 |
Subcellular locationi
Cytosol
- cytosol Source: GO_Central
Nucleus
- nucleus Source: GO_Central
Other locations
- SCF ubiquitin ligase complex Source: GO_Central
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 91 | C → A: No FMN binding and decreased interaction with GI. 2 Publications | 1 | |
Mutagenesisi | 92 | R → D: Decreased interaction with GI. 1 Publication | 1 | |
Mutagenesisi | 163 | Q → L: Decreased interaction with GI. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119958 | 1 – 619 | Adagio protein 3Add BLAST | 619 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 91 | S-4a-FMN cysteine | 1 |
Post-translational modificationi
Proteomic databases
PaxDbi | Q9C9W9 |
ProteomicsDBi | 244875 |
PTM databases
iPTMneti | Q9C9W9 |
Expressioni
Tissue specificityi
Inductioni
Gene expression databases
ExpressionAtlasi | Q9C9W9, baseline and differential |
Genevisiblei | Q9C9W9, AT |
Interactioni
Subunit structurei
Interacts with ADO1 (via Kelch repeats), ADO2 (via Kelch repeats), SKP1A/ASK1, SKP1B/ASK2, ASK3, SKP1K/ASK11, ASK12, ASK13 and SKP1N/ASK14.
Interacts (via Kelch repeats) with CDF1, CDF2 and CDF3.
Interacts (via N-terminus) with CO and GI (via N-terminus) in a blue-light-dependent manner.
7 PublicationsBinary interactionsi
Q9C9W9
Protein-protein interaction databases
BioGRIDi | 28354, 24 interactors |
DIPi | DIP-31333N |
IntActi | Q9C9W9, 16 interactors |
STRINGi | 3702.AT1G68050.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 44 – 123 | PASPROSITE-ProRule annotationAdd BLAST | 80 | |
Domaini | 127 – 168 | PACAdd BLAST | 42 | |
Domaini | 211 – 257 | F-boxAdd BLAST | 47 | |
Repeati | 304 – 354 | Kelch 1Add BLAST | 51 | |
Repeati | 357 – 404 | Kelch 2Add BLAST | 48 | |
Repeati | 409 – 457 | Kelch 3Add BLAST | 49 | |
Repeati | 462 – 513 | Kelch 4Add BLAST | 52 | |
Repeati | 523 – 571 | Kelch 5Add BLAST | 49 |
Sequence similaritiesi
Keywords - Domaini
Kelch repeat, RepeatPhylogenomic databases
eggNOGi | ENOG502QWBU, Eukaryota |
HOGENOMi | CLU_033494_1_0_1 |
InParanoidi | Q9C9W9 |
OMAi | AVCWRKF |
OrthoDBi | 466221at2759 |
PhylomeDBi | Q9C9W9 |
Family and domain databases
CDDi | cd00130, PAS, 1 hit |
Gene3Di | 2.120.10.80, 2 hits |
InterProi | View protein in InterPro IPR036047, F-box-like_dom_sf IPR015915, Kelch-typ_b-propeller IPR011498, Kelch_2 IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf |
Pfami | View protein in Pfam PF07646, Kelch_2, 1 hit PF13426, PAS_9, 1 hit |
SMARTi | View protein in SMART SM00086, PAC, 1 hit |
SUPFAMi | SSF117281, SSF117281, 2 hits SSF55785, SSF55785, 1 hit SSF81383, SSF81383, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50112, PAS, 1 hit |
i Sequence
Sequence statusi: Complete.
10 20 30 40 50
MAREHAIGEA TGKRKKRGRV EEAEEYCNDG IEEQVEDEKL PLEVGMFYYP
60 70 80 90 100
MTPPSFIVSD ALEPDFPLIY VNRVFEVFTG YRADEVLGRN CRFLQYRDPR
110 120 130 140 150
AQRRHPLVDP VVVSEIRRCL EEGIEFQGEL LNFRKDGTPL VNRLRLAPIR
160 170 180 190 200
DDDGTITHVI GIQVFSETTI DLDRVSYPVF KHKQQLDQTS ECLFPSGSPR
210 220 230 240 250
FKEHHEDFCG ILQLSDEVLA HNILSRLTPR DVASIGSACR RLRQLTKNES
260 270 280 290 300
VRKMVCQNAW GKEITGTLEI MTKKLRWGRL ARELTTLEAV CWRKFTVGGI
310 320 330 340 350
VQPSRCNFSA CAVGNRLVLF GGEGVNMQPL DDTFVLNLDA ECPEWQRVRV
360 370 380 390 400
TSSPPGRWGH TLSCLNGSWL VVFGGCGRQG LLNDVFVLDL DAKHPTWKEV
410 420 430 440 450
AGGTPPLPRS WHSSCTIEGS KLVVSGGCTD AGVLLSDTFL LDLTTDKPTW
460 470 480 490 500
KEIPTSWAPP SRLGHSLSVF GRTKILMFGG LANSGHLKLR SGEAYTIDLE
510 520 530 540 550
DEEPRWRELE CSAFPGVVVP PPRLDHVAVS MPCGRVIIFG GSIAGLHSPS
560 570 580 590 600
QLFLIDPAEE KPSWRILNVP GKPPKLAWGH STCVVGGTRV LVLGGHTGEE
610
WILNELHELC LASRQDSDL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 484 | S → I in AAF32298 (PubMed:10847687).Curated | 1 | |
Sequence conflicti | 484 | S → I in AAK27435 (PubMed:11260718).Curated | 1 | |
Sequence conflicti | 581 | S → N in AAF32298 (PubMed:10847687).Curated | 1 | |
Sequence conflicti | 581 | S → N in AAK27435 (PubMed:11260718).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF216523 mRNA Translation: AAF32298.2 AF252296 mRNA Translation: AAK27435.1 AC012563 Genomic DNA Translation: AAG51994.1 CP002684 Genomic DNA Translation: AEE34741.1 AY064999 mRNA Translation: AAL57647.1 AY113029 mRNA Translation: AAM47337.1 |
PIRi | F96703 |
RefSeqi | NP_564919.1, NM_105475.4 |
Genome annotation databases
EnsemblPlantsi | AT1G68050.1; AT1G68050.1; AT1G68050 |
GeneIDi | 843133 |
Gramenei | AT1G68050.1; AT1G68050.1; AT1G68050 |
KEGGi | ath:AT1G68050 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF216523 mRNA Translation: AAF32298.2 AF252296 mRNA Translation: AAK27435.1 AC012563 Genomic DNA Translation: AAG51994.1 CP002684 Genomic DNA Translation: AEE34741.1 AY064999 mRNA Translation: AAL57647.1 AY113029 mRNA Translation: AAM47337.1 |
PIRi | F96703 |
RefSeqi | NP_564919.1, NM_105475.4 |
3D structure databases
AlphaFoldDBi | Q9C9W9 |
SMRi | Q9C9W9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 28354, 24 interactors |
DIPi | DIP-31333N |
IntActi | Q9C9W9, 16 interactors |
STRINGi | 3702.AT1G68050.1 |
PTM databases
iPTMneti | Q9C9W9 |
Proteomic databases
PaxDbi | Q9C9W9 |
ProteomicsDBi | 244875 |
Genome annotation databases
EnsemblPlantsi | AT1G68050.1; AT1G68050.1; AT1G68050 |
GeneIDi | 843133 |
Gramenei | AT1G68050.1; AT1G68050.1; AT1G68050 |
KEGGi | ath:AT1G68050 |
Organism-specific databases
Araporti | AT1G68050 |
TAIRi | locus:2200176, AT1G68050 |
Phylogenomic databases
eggNOGi | ENOG502QWBU, Eukaryota |
HOGENOMi | CLU_033494_1_0_1 |
InParanoidi | Q9C9W9 |
OMAi | AVCWRKF |
OrthoDBi | 466221at2759 |
PhylomeDBi | Q9C9W9 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
Miscellaneous databases
PROi | PR:Q9C9W9 |
Gene expression databases
ExpressionAtlasi | Q9C9W9, baseline and differential |
Genevisiblei | Q9C9W9, AT |
Family and domain databases
CDDi | cd00130, PAS, 1 hit |
Gene3Di | 2.120.10.80, 2 hits |
InterProi | View protein in InterPro IPR036047, F-box-like_dom_sf IPR015915, Kelch-typ_b-propeller IPR011498, Kelch_2 IPR001610, PAC IPR000014, PAS IPR035965, PAS-like_dom_sf |
Pfami | View protein in Pfam PF07646, Kelch_2, 1 hit PF13426, PAS_9, 1 hit |
SMARTi | View protein in SMART SM00086, PAC, 1 hit |
SUPFAMi | SSF117281, SSF117281, 2 hits SSF55785, SSF55785, 1 hit SSF81383, SSF81383, 1 hit |
TIGRFAMsi | TIGR00229, sensory_box, 1 hit |
PROSITEi | View protein in PROSITE PS50112, PAS, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ADO3_ARATH | |
Accessioni | Q9C9W9Primary (citable) accession number: Q9C9W9 Secondary accession number(s): Q9M648 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 8, 2005 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 143 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families