UniProtKB - Q9C9P4 (KASC2_ARATH)
3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic
KAS2
Functioni
Essential protein that catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-16 and C-16 to unsaturated C-18 fatty acids. Confers resistance to low temperatures by maintaining chloroplast membranes integrity. Involved in the regulation of fatty acids ratios during seed metabolism. Required for embryo development, especially at the transition from the globular to the heart stage.
6 PublicationsCatalytic activityi
- EC:2.3.1.411 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 292 | PROSITE-ProRule annotation | 1 |
GO - Molecular functioni
- 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: TAIR
GO - Biological processi
- cold acclimation Source: UniProtKB
- embryo development ending in seed dormancy Source: UniProtKB
- fatty acid biosynthetic process Source: TAIR
- unsaturated fatty acid biosynthetic process Source: TAIR
Keywordsi
Molecular function | Acyltransferase, Developmental protein, Transferase |
Biological process | Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism |
Enzyme and pathway databases
BioCyci | ARA:MONOMER-14118 |
BRENDAi | 2.3.1.179, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: 3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplastic (EC:2.3.1.41)Alternative name(s): Beta-ketoacyl-acyl-carrier-protein synthase II Short name: AtKAS2 Short name: Beta-ketoacyl-ACP synthetase 2 Protein FATTY ACID BIOSYNTHESIS 1 |
Gene namesi | Name:KAS2 Synonyms:FAB1 Ordered Locus Names:At1g74960 ORF Names:F25A4.7, F9E10.19 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G74960 |
TAIRi | locus:2027252, AT1G74960 |
Subcellular locationi
Chloroplast
- chloroplast stroma 1 Publication
Chloroplast
- chloroplast Source: TAIR
- chloroplast stroma Source: TAIR
Cytosol
- cytosol Source: TAIR
Other locations
- plastid Source: TAIR
Keywords - Cellular componenti
Chloroplast, PlastidPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 337 | L → F in fab1; partial activity deficiency due to structural instability and reduced substrate binding affinity, resulting in increased levels of saturated 16:0 but reduced levels of 18:0 fatty acids, particularly in chloroplasts, and associated with damage and death at continuous low temperature (accompanied by chloroplast degenerescence), but not after transient chilling or freezing. 5 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 103 | ChloroplastSequence analysisAdd BLAST | 103 | |
ChainiPRO_0000406094 | 104 – 541 | 3-oxoacyl-[acyl-carrier-protein] synthase II, chloroplasticAdd BLAST | 438 |
Proteomic databases
PaxDbi | Q9C9P4 |
PRIDEi | Q9C9P4 |
ProteomicsDBi | 250617 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
ExpressionAtlasi | Q9C9P4, baseline and differential |
Genevisiblei | Q9C9P4, AT |
Interactioni
Subunit structurei
Homodimer.
By similarityProtein-protein interaction databases
BioGRIDi | 29054, 1 interactor |
STRINGi | 3702.AT1G74960.2 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1394, Eukaryota |
HOGENOMi | CLU_000022_69_1_1 |
InParanoidi | Q9C9P4 |
OMAi | PKFSRRM |
OrthoDBi | 1014523at2759 |
PhylomeDBi | Q9C9P4 |
Family and domain databases
CDDi | cd00834, KAS_I_II, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR017568, 3-oxoacyl-ACP_synth-2 IPR000794, Beta-ketoacyl_synthase IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR020841, PKS_Beta-ketoAc_synthase_dom IPR016039, Thiolase-like |
PANTHERi | PTHR11712, PTHR11712, 1 hit |
Pfami | View protein in Pfam PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit |
SMARTi | View protein in SMART SM00825, PKS_KS, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR03150, fabF, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
10 20 30 40 50
MVGASSSYAS PLCTWFVAAC MSVSHGGGDS RQAVALQSGG RSRRRRQLSK
60 70 80 90 100
CSVASGSASI QALVTSCLDF GPCTHYNNNN ALSSLFGSNS VSLNRNQRRL
110 120 130 140 150
NRAASSGGAM AVMEMEKEAA VNKKPPTEQR RVVVTGMGVE TSLGHDPHTF
160 170 180 190 200
YENLLQGNSG ISQIENFDCS EFPTRIAGEI KSFSTEGWVA PKLSKRMDKF
210 220 230 240 250
MLYLLTAGKK ALADGGVTDE VMAEFDKTKC GVLIGSAMGG MKVFYDAIEA
260 270 280 290 300
LRISYKKMNP FCVPFATTNM GSAMLAMDLG WMGPNYSIST ACATSNFCIL
310 320 330 340 350
NSANHIIKGE ADVMLCGGSD AVIIPIGLGG FVACRALSQR NNDPTKASRP
360 370 380 390 400
WDTNRDGFVM GEGAGVLLLE ELEHAKKRGA TIYAEFLGGS FTCDAYHMTE
410 420 430 440 450
PHPDGAGVIL CIERALASAG ISKEQINYIN AHATSTHAGD IKEYQALAHC
460 470 480 490 500
FGQNPELKVN STKSMIGHLL GAAGAVEAVA TVQAIRTGWV HPNINLENPD
510 520 530 540
SGVDTKLLVG PKKERLDIKA ALSNSFGFGG HNSSIIFAPY K
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 472 | A → V in AAL91174 (PubMed:14593172).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF318307 mRNA Translation: AAK69603.1 AC008263 Genomic DNA Translation: AAD55280.1 Sequence problems. AC013258 Genomic DNA Translation: AAG51920.1 CP002684 Genomic DNA Translation: AEE35655.1 CP002684 Genomic DNA Translation: AEE35656.1 CP002684 Genomic DNA Translation: AEE35657.1 AF412045 mRNA Translation: AAL06498.1 Frameshift. AF419598 mRNA Translation: AAL31930.1 AY054196 mRNA Translation: AAL06857.1 AY081285 mRNA Translation: AAL91174.1 AY097344 mRNA Translation: AAM19860.1 |
PIRi | D96779 |
RefSeqi | NP_001185400.1, NM_001198471.1 NP_565097.1, NM_106154.4 NP_849888.1, NM_179557.4 |
Genome annotation databases
EnsemblPlantsi | AT1G74960.1; AT1G74960.1; AT1G74960 AT1G74960.2; AT1G74960.2; AT1G74960 AT1G74960.3; AT1G74960.3; AT1G74960 |
GeneIDi | 843835 |
Gramenei | AT1G74960.1; AT1G74960.1; AT1G74960 AT1G74960.2; AT1G74960.2; AT1G74960 AT1G74960.3; AT1G74960.3; AT1G74960 |
KEGGi | ath:AT1G74960 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF318307 mRNA Translation: AAK69603.1 AC008263 Genomic DNA Translation: AAD55280.1 Sequence problems. AC013258 Genomic DNA Translation: AAG51920.1 CP002684 Genomic DNA Translation: AEE35655.1 CP002684 Genomic DNA Translation: AEE35656.1 CP002684 Genomic DNA Translation: AEE35657.1 AF412045 mRNA Translation: AAL06498.1 Frameshift. AF419598 mRNA Translation: AAL31930.1 AY054196 mRNA Translation: AAL06857.1 AY081285 mRNA Translation: AAL91174.1 AY097344 mRNA Translation: AAM19860.1 |
PIRi | D96779 |
RefSeqi | NP_001185400.1, NM_001198471.1 NP_565097.1, NM_106154.4 NP_849888.1, NM_179557.4 |
3D structure databases
SMRi | Q9C9P4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 29054, 1 interactor |
STRINGi | 3702.AT1G74960.2 |
Proteomic databases
PaxDbi | Q9C9P4 |
PRIDEi | Q9C9P4 |
ProteomicsDBi | 250617 |
Genome annotation databases
EnsemblPlantsi | AT1G74960.1; AT1G74960.1; AT1G74960 AT1G74960.2; AT1G74960.2; AT1G74960 AT1G74960.3; AT1G74960.3; AT1G74960 |
GeneIDi | 843835 |
Gramenei | AT1G74960.1; AT1G74960.1; AT1G74960 AT1G74960.2; AT1G74960.2; AT1G74960 AT1G74960.3; AT1G74960.3; AT1G74960 |
KEGGi | ath:AT1G74960 |
Organism-specific databases
Araporti | AT1G74960 |
TAIRi | locus:2027252, AT1G74960 |
Phylogenomic databases
eggNOGi | KOG1394, Eukaryota |
HOGENOMi | CLU_000022_69_1_1 |
InParanoidi | Q9C9P4 |
OMAi | PKFSRRM |
OrthoDBi | 1014523at2759 |
PhylomeDBi | Q9C9P4 |
Enzyme and pathway databases
BioCyci | ARA:MONOMER-14118 |
BRENDAi | 2.3.1.179, 399 |
Miscellaneous databases
PROi | PR:Q9C9P4 |
Gene expression databases
ExpressionAtlasi | Q9C9P4, baseline and differential |
Genevisiblei | Q9C9P4, AT |
Family and domain databases
CDDi | cd00834, KAS_I_II, 1 hit |
Gene3Di | 3.40.47.10, 1 hit |
InterProi | View protein in InterPro IPR017568, 3-oxoacyl-ACP_synth-2 IPR000794, Beta-ketoacyl_synthase IPR018201, Ketoacyl_synth_AS IPR014031, Ketoacyl_synth_C IPR014030, Ketoacyl_synth_N IPR020841, PKS_Beta-ketoAc_synthase_dom IPR016039, Thiolase-like |
PANTHERi | PTHR11712, PTHR11712, 1 hit |
Pfami | View protein in Pfam PF00109, ketoacyl-synt, 1 hit PF02801, Ketoacyl-synt_C, 1 hit |
SMARTi | View protein in SMART SM00825, PKS_KS, 1 hit |
SUPFAMi | SSF53901, SSF53901, 2 hits |
TIGRFAMsi | TIGR03150, fabF, 1 hit |
PROSITEi | View protein in PROSITE PS00606, B_KETOACYL_SYNTHASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KASC2_ARATH | |
Accessioni | Q9C9P4Primary (citable) accession number: Q9C9P4 Secondary accession number(s): Q8RXF5, Q945N5, Q9SSG8 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 8, 2011 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 134 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families