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Protein

E3 ubiquitin-protein ligase SINA-like 4

Gene

At1g66650

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi144Zinc 1By similarity1
Metal bindingi151Zinc 1By similarity1
Metal bindingi164Zinc 1By similarity1
Metal bindingi168Zinc 1By similarity1
Metal bindingi175Zinc 2By similarity1
Metal bindingi180Zinc 2By similarity1
Metal bindingi192Zinc 2By similarity1
Metal bindingi197Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri86 – 122RING-type; degenerateAdd BLAST37
Zinc fingeri139 – 198SIAH-typePROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SINA-like 4 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase SINA-like 4Curated
Seven in absentia-like protein 4
Gene namesi
Ordered Locus Names:At1g66650
ORF Names:F4N21_21, T12I7.10
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G66650
TAIRilocus:2033369 AT1G66650

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002991931 – 329E3 ubiquitin-protein ligase SINA-like 4Add BLAST329

Proteomic databases

PaxDbiQ9C9M0
PRIDEiQ9C9M0

Expressioni

Gene expression databases

ExpressionAtlasiQ9C9M0 baseline and differential
GenevisibleiQ9C9M0 AT

Interactioni

Protein-protein interaction databases

BioGridi28204, 4 interactors
IntActiQ9C9M0, 1 interactor
STRINGi3702.AT1G66650.1

Structurei

3D structure databases

ProteinModelPortaliQ9C9M0
SMRiQ9C9M0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni136 – 325SBDBy similarityAdd BLAST190

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri86 – 122RING-type; degenerateAdd BLAST37
Zinc fingeri139 – 198SIAH-typePROSITE-ProRule annotationAdd BLAST60

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000154324
InParanoidiQ9C9M0
KOiK04506
OMAiYGCKQST
OrthoDBiEOG09360IVP
PhylomeDBiQ9C9M0

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS51081 ZF_SIAH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9C9M0-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MTKLGRRNDG GGKSHRSSTK RQRRTSVSVD DPSPGEEEEK TLVVLTDDSD
60 70 80 90 100
SEEDDKPLGD VLRTCRKRRV SSPKSVTLPN SNVLECPNCF DPLKKPIFQC
110 120 130 140 150
NNGHLACFLC CIKLKKRCSF CKLPIGDVRC RAMEKVIKAG LVSCSNAIYG
160 170 180 190 200
CKQSTTYGNQ LQSHEKVCVF APCSCPIKDC NYIGFYKDLI NHFRATHKVS
210 220 230 240 250
PGDINSFVFD RPVIFGLDLD SSDKMVIFVE EKQGNLFVVQ GFIGSHGVYA
260 270 280 290 300
TVSHIAPMVP EVRKFSCSLA RLRPYSTLRL GLEVKNIQKL RSQEEQPQED
310 320
FLLIPSYMVN GDHMKMEISI GDKNDYTHI
Length:329
Mass (Da):36,938
Last modified:June 1, 2001 - v1
Checksum:i6E08A5C15C92A746
GO

Sequence cautioni

The sequence AAG51169 differs from that shown. Reason: Erroneous gene model prediction.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013288 Genomic DNA Translation: AAG60080.1
AC079285 Genomic DNA Translation: AAG51169.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34540.1
RefSeqiNP_849853.1, NM_179522.2
UniGeneiAt.35744

Genome annotation databases

EnsemblPlantsiAT1G66650.1; AT1G66650.1; AT1G66650
GeneIDi842983
GrameneiAT1G66650.1; AT1G66650.1; AT1G66650
KEGGiath:AT1G66650

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC013288 Genomic DNA Translation: AAG60080.1
AC079285 Genomic DNA Translation: AAG51169.1 Sequence problems.
CP002684 Genomic DNA Translation: AEE34540.1
RefSeqiNP_849853.1, NM_179522.2
UniGeneiAt.35744

3D structure databases

ProteinModelPortaliQ9C9M0
SMRiQ9C9M0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi28204, 4 interactors
IntActiQ9C9M0, 1 interactor
STRINGi3702.AT1G66650.1

Proteomic databases

PaxDbiQ9C9M0
PRIDEiQ9C9M0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66650.1; AT1G66650.1; AT1G66650
GeneIDi842983
GrameneiAT1G66650.1; AT1G66650.1; AT1G66650
KEGGiath:AT1G66650

Organism-specific databases

AraportiAT1G66650
TAIRilocus:2033369 AT1G66650

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000154324
InParanoidiQ9C9M0
KOiK04506
OMAiYGCKQST
OrthoDBiEOG09360IVP
PhylomeDBiQ9C9M0

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q9C9M0

Gene expression databases

ExpressionAtlasiQ9C9M0 baseline and differential
GenevisibleiQ9C9M0 AT

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSINL4_ARATH
AccessioniPrimary (citable) accession number: Q9C9M0
Secondary accession number(s): Q9C6H0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 109 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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