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Protein

3-deoxy-manno-octulosonate cytidylyltransferase, mitochondrial

Gene

KDSB

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the production of the sugar nucleotide CMP-3-deoxy-D-manno-octulosonate (CMP-KDO). CTP is the preferred nucleotide donor, but it can partially be replaced with UTP. Activates KDO during the biosynthesis of rhamnogalacturonan II (RG-II), a structurally complex pectic polysaccharide of the primary cell wall. RG-II is essential for the cell wall integrity of rapidly growing tissues and pollen tube growth and elongation.2 Publications

Miscellaneous

Rhamnogalacturonan II (RG-II) RG-II is a structurally complex pectic polysaccharide present in the primary cell wall. RG-II consists of a linear 1,4-linked a-Dgalacturonic acid backbone with four distinct side chains, two of which contain apiosyl residues. Boric acid forms a diester with two apiosyl residues from separate RG-II molecules, thereby covalently cross-linking two RG-II molecules as a dimer.

Catalytic activityi

CTP + 3-deoxy-D-manno-octulosonate = diphosphate + CMP-3-deoxy-D-manno-octulosonate.1 Publication

Cofactori

Mg2+1 Publication

Enzyme regulationi

Inhibited by 2beta-deoxy-Kdo.1 Publication

Kineticsi

kcat is 8.77 sec(-1) for 3-deoxy-D-manno-octulosonate.1 Publication
  1. KM=88.59 µM for 3-deoxy-D-manno-octulosonate1 Publication
  1. Vmax=14.62 µmol/min/µg enzyme1 Publication

pH dependencei

Optimum pH is 9.5.1 Publication

Pathwayi: CMP-3-deoxy-D-manno-octulosonate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP.Curated
Proteins known to be involved in this subpathway in this organism are:
  1. 3-deoxy-manno-octulosonate cytidylyltransferase, mitochondrial (KDSB)
This subpathway is part of the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis, which is itself part of Nucleotide-sugar biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes CMP-3-deoxy-D-manno-octulosonate from 3-deoxy-D-manno-octulosonate and CTP, the pathway CMP-3-deoxy-D-manno-octulosonate biosynthesis and in Nucleotide-sugar biosynthesis.

GO - Molecular functioni

  • 3-deoxy-manno-octulosonate cytidylyltransferase activity Source: TAIR

GO - Biological processi

  • cell wall organization Source: UniProtKB-KW
  • CMP-keto-3-deoxy-D-manno-octulosonic acid biosynthetic process Source: TAIR
  • pollen development Source: TAIR
  • pollen tube growth Source: TAIR

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processCell wall biogenesis/degradation
LigandMagnesium

Enzyme and pathway databases

BRENDAi2.7.7.38 399
UniPathwayiUPA00358; UER00476

Names & Taxonomyi

Protein namesi
Recommended name:
3-deoxy-manno-octulosonate cytidylyltransferase, mitochondrial1 Publication (EC:2.7.7.381 Publication)
Alternative name(s):
CMP-2-keto-3-deoxyoctulosonic acid synthase1 Publication
Short name:
AtCKS1 Publication
Short name:
CMP-KDO synthase1 Publication
Gene namesi
Name:KDSBCurated
Synonyms:CKS1 Publication, KDO11 Publication
Ordered Locus Names:At1g53000Imported
ORF Names:F14G24.28Imported
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G53000
TAIRilocus:2037015 AT1G53000

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion outer membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50MitochondrionSequence analysisAdd BLAST50
ChainiPRO_000042146451 – 2903-deoxy-manno-octulosonate cytidylyltransferase, mitochondrialAdd BLAST240

Proteomic databases

PaxDbiQ9C920
PRIDEiQ9C920

PTM databases

SwissPalmiQ9C920

Expressioni

Tissue specificityi

Expressed in roots, leaves, stems and siliques.1 Publication

Gene expression databases

ExpressionAtlasiQ9C920 baseline and differential
GenevisibleiQ9C920 AT

Interactioni

Protein-protein interaction databases

BioGridi26958, 4 interactors
STRINGi3702.AT1G53000.1

Structurei

3D structure databases

ProteinModelPortaliQ9C920
SMRiQ9C920
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the KdsB family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiENOG410IHX0 Eukaryota
COG1212 LUCA
HOGENOMiHOG000007602
InParanoidiQ9C920
KOiK00979
OMAiNSGTERC
OrthoDBiEOG09360IZ8
PhylomeDBiQ9C920

Family and domain databases

CDDicd02517 CMP-KDO-Synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_00057 KdsB, 1 hit
InterProiView protein in InterPro
IPR003329 Cytidylyl_trans
IPR004528 KdsB
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF02348 CTP_transf_3, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR00466 kdsB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9C920-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSVCSSSSSS QKTWIVNGIL AGTAIAAAIG ARAYLGRSKK FRSRVVGIIP
60 70 80 90 100
ARYASSRFEG KPLVQILGKP MIQRTWERSK LATTLDHIVV ATDDERIAEC
110 120 130 140 150
CRGFGADVIM TSESCRNGTE RCNEALEKLE KKYDVVVNIQ GDEPLIEPEI
160 170 180 190 200
IDGVVKALQV TPDAVFSTAV TSLKPEDGLD PNRVKCVVDN RGYAIYFSRG
210 220 230 240 250
LIPYNKSGKV NPDFPYMLHL GIQSFDSKFL KVYSELQPTP LQQEEDLEQL
260 270 280 290
KVLENGYKMK VIKVDHEAHG VDTPDDVEKI ESLMRERNMS
Length:290
Mass (Da):32,245
Last modified:June 1, 2001 - v1
Checksum:i7A6AE112A3D51E62
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ505021 mRNA Translation: CAD43603.1
AC019018 Genomic DNA Translation: AAG52266.1
CP002684 Genomic DNA Translation: AEE32875.1
AK317553 mRNA Translation: BAH20217.1
RefSeqiNP_175708.2, NM_104178.5
UniGeneiAt.18072

Genome annotation databases

EnsemblPlantsiAT1G53000.1; AT1G53000.1; AT1G53000
GeneIDi841733
GrameneiAT1G53000.1; AT1G53000.1; AT1G53000
KEGGiath:AT1G53000

Similar proteinsi

Entry informationi

Entry nameiKDSB_ARATH
AccessioniPrimary (citable) accession number: Q9C920
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2013
Last sequence update: June 1, 2001
Last modified: May 23, 2018
This is version 108 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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