UniProtKB - Q9C8L4 (ETHE1_ARATH)
Protein
Persulfide dioxygenase ETHE1 homolog, mitochondrial
Gene
GLY3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Sulfur dioxygenase that plays an essential role in hydrogen sulfide catabolism in the mitochondrial matrix. Hydrogen sulfide (H2S) gives rise to cysteine persulfide residues. ETHE1 consumes molecular oxygen to catalyze the oxidation of the persulfide, once it has been transferred to a thiophilic acceptor, such as glutathione (R-SSH). Plays an important role in metabolic homeostasis in mitochondria by metabolizing hydrogen sulfide and preventing the accumulation of supraphysiological H2S levels that have toxic effects, due to the inhibition of cytochrome c oxidase. Required for normal endosperm development in seed, and thereby also required for normal embryo development.1 Publication
Caution
Was initially thought to be a glyoxalase II isozyme (PubMed:9349270), but has been shown to lack glyoxalase activity (PubMed:22786886).2 Publications
Catalytic activityi
- EC:1.13.11.181 Publication
Cofactori
Fe2+1 PublicationNote: Binds 1 Fe2+ ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 110 | Iron; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 166 | Iron; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 191 | Iron; catalyticCombined sources1 Publication | 1 |
GO - Molecular functioni
- hydrolase activity, acting on ester bonds Source: TAIR
- metal ion binding Source: UniProtKB-KW
- sulfur dioxygenase activity Source: TAIR
GO - Biological processi
- embryo development ending in seed dormancy Source: TAIR
- endosperm development Source: TAIR
- glutathione metabolic process Source: GO_Central
- hydrogen sulfide metabolic process Source: GO_Central
- seed development Source: TAIR
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BRENDAi | 1.13.11.18, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Persulfide dioxygenase ETHE1 homolog, mitochondrial (EC:1.13.11.181 Publication)Alternative name(s): Glyoxalase II Short name: Glx II Sulfur dioxygenase ETHE1 |
Gene namesi | Name:GLY3 Ordered Locus Names:At1g53580 ORF Names:F22G10.9, T3F20.11 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G53580 |
TAIRi | locus:2024922, AT1G53580 |
Subcellular locationi
Mitochondrion
- Mitochondrion 1 Publication
Mitochondrion
- mitochondrion Source: TAIR
Nucleus
- nucleus Source: TAIR
Keywords - Cellular componenti
MitochondrionPathology & Biotechi
Disruption phenotypei
Embryonic lethality at the early heart stage, due to defects in endosperm development.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 39 | MitochondrionCombined sourcesAdd BLAST | 39 | |
ChainiPRO_0000012288 | 40 – 294 | Persulfide dioxygenase ETHE1 homolog, mitochondrialAdd BLAST | 255 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 40 | N-acetylglycineCombined sources | 1 |
Keywords - PTMi
AcetylationProteomic databases
PaxDbi | Q9C8L4 |
PRIDEi | Q9C8L4 |
ProteomicsDBi | 222296 [Q9C8L4-1] |
PTM databases
iPTMneti | Q9C8L4 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9C8L4, baseline and differential |
Genevisiblei | Q9C8L4, AT |
Interactioni
Subunit structurei
Homodimer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 27018, 1 interactor |
STRINGi | 3702.AT1G53580.1 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9C8L4 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9C8L4 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG0814, Eukaryota |
HOGENOMi | CLU_030571_7_0_1 |
InParanoidi | Q9C8L4 |
OMAi | CGIQTPP |
OrthoDBi | 1203911at2759 |
Family and domain databases
Gene3Di | 3.60.15.10, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF00753, Lactamase_B, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 1 produced by isoform ialternative splicing. AlignAdd to basketNote: A number of isoforms are produced. According to EST sequences.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9C8L4-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MVMTHFSRLR QLLLLQPKFL SSQPRPLRSP PPTFLRSVMG SSSSFSSSSS
60 70 80 90 100
KLLFRQLFEN ESSTFTYLLA DVSHPDKPAL LIDPVDKTVD RDLKLIDELG
110 120 130 140 150
LKLIYAMNTH VHADHVTGTG LLKTKLPGVK SVISKASGSK ADLFLEPGDK
160 170 180 190 200
VSIGDIYLEV RATPGHTAGC VTYVTGEGAD QPQPRMAFTG DAVLIRGCGR
210 220 230 240 250
TDFQEGSSDQ LYESVHSQIF TLPKDTLIYP AHDYKGFEVS TVGEEMQHNP
260 270 280 290
RLTKDKETFK TIMSNLNLSY PKMIDVAVPA NMVCGLQDVP SQAN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF4HRK0 | F4HRK0_ARATH | Glyoxalase II 3 | GLY3 ETHE1, ETHE1-LIKE, GLX2-3, GLYOXALASE 2-3, glyoxalase II 3 | 294 | Annotation score: |
Sequence cautioni
The sequence AAB17995 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAF78432 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence AAG51989 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAK96491 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
The sequence AAL25570 differs from that shown. Reason: Erroneous initiation. Truncated N-terminus.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 38 | V → L in AAB17995 (Ref. 4) Curated | 1 | |
Sequence conflicti | 205 | E → G in AAB17995 (Ref. 4) Curated | 1 | |
Sequence conflicti | 221 | T → S in AAB17995 (Ref. 4) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC018748 Genomic DNA Translation: AAF78432.1 Sequence problems. AC024260 Genomic DNA Translation: AAG51989.1 Different initiation. CP002684 Genomic DNA Translation: AEE32965.1 AY058154 mRNA Translation: AAL25570.1 Different initiation. AY052298 mRNA Translation: AAK96491.1 Different initiation. AY139809 mRNA Translation: AAM98115.1 U74610 mRNA Translation: AAB17995.1 Different initiation. |
PIRi | H96575 |
RefSeqi | NP_564636.2, NM_104236.5 [Q9C8L4-1] |
Genome annotation databases
EnsemblPlantsi | AT1G53580.1; AT1G53580.1; AT1G53580 [Q9C8L4-1] |
GeneIDi | 841793 |
Gramenei | AT1G53580.1; AT1G53580.1; AT1G53580 [Q9C8L4-1] |
KEGGi | ath:AT1G53580 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC018748 Genomic DNA Translation: AAF78432.1 Sequence problems. AC024260 Genomic DNA Translation: AAG51989.1 Different initiation. CP002684 Genomic DNA Translation: AEE32965.1 AY058154 mRNA Translation: AAL25570.1 Different initiation. AY052298 mRNA Translation: AAK96491.1 Different initiation. AY139809 mRNA Translation: AAM98115.1 U74610 mRNA Translation: AAB17995.1 Different initiation. |
PIRi | H96575 |
RefSeqi | NP_564636.2, NM_104236.5 [Q9C8L4-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2GCU | X-ray | 1.48 | A/B/C/D | 51-293 | [»] | |
SMRi | Q9C8L4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 27018, 1 interactor |
STRINGi | 3702.AT1G53580.1 |
PTM databases
iPTMneti | Q9C8L4 |
Proteomic databases
PaxDbi | Q9C8L4 |
PRIDEi | Q9C8L4 |
ProteomicsDBi | 222296 [Q9C8L4-1] |
Genome annotation databases
EnsemblPlantsi | AT1G53580.1; AT1G53580.1; AT1G53580 [Q9C8L4-1] |
GeneIDi | 841793 |
Gramenei | AT1G53580.1; AT1G53580.1; AT1G53580 [Q9C8L4-1] |
KEGGi | ath:AT1G53580 |
Organism-specific databases
Araporti | AT1G53580 |
TAIRi | locus:2024922, AT1G53580 |
Phylogenomic databases
eggNOGi | KOG0814, Eukaryota |
HOGENOMi | CLU_030571_7_0_1 |
InParanoidi | Q9C8L4 |
OMAi | CGIQTPP |
OrthoDBi | 1203911at2759 |
Enzyme and pathway databases
BRENDAi | 1.13.11.18, 399 |
Miscellaneous databases
EvolutionaryTracei | Q9C8L4 |
PROi | PR:Q9C8L4 |
Gene expression databases
ExpressionAtlasi | Q9C8L4, baseline and differential |
Genevisiblei | Q9C8L4, AT |
Family and domain databases
Gene3Di | 3.60.15.10, 1 hit |
InterProi | View protein in InterPro IPR001279, Metallo-B-lactamas IPR036866, RibonucZ/Hydroxyglut_hydro |
Pfami | View protein in Pfam PF00753, Lactamase_B, 1 hit |
SMARTi | View protein in SMART SM00849, Lactamase_B, 1 hit |
SUPFAMi | SSF56281, SSF56281, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ETHE1_ARATH | |
Accessioni | Q9C8L4Primary (citable) accession number: Q9C8L4 Secondary accession number(s): Q93VR2, Q96332, Q9LPH3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 16, 2005 |
Last sequence update: | May 31, 2011 | |
Last modified: | April 7, 2021 | |
This is version 127 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families