UniProtKB - Q9C6Z1 (NCED5_ARATH)
Protein
Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic
Gene
NCED5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Has a 11,12(11',12') 9-cis epoxycarotenoid cleavage activity. Catalyzes the first step of abscisic-acid biosynthesis from carotenoids (By similarity).By similarity
Catalytic activityi
- EC:1.13.11.51
- 9-cis-violaxanthin + O2 = (3S,5R,6S)-5,6-epoxy-3-hydroxy-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinEC:1.13.11.51
- 9'-cis-neoxanthin + O2 = (3S,5R,6R)-3,5-dihydroxy-6,7-didehydro-5,6-dihydro-12'-apo-β-caroten-12'-al + 2-cis,4-trans-xanthoxinEC:1.13.11.51
Cofactori
Fe2+By similarityNote: Binds 1 Fe2+ ion per subunit.By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 287 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 336 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 401 | Iron; via tele nitrogenBy similarity | 1 | |
Metal bindingi | 576 | Iron; via tele nitrogenBy similarity | 1 |
GO - Molecular functioni
- 9-cis-epoxycarotenoid dioxygenase activity Source: TAIR
- carotenoid dioxygenase activity Source: GO_Central
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- abscisic acid biosynthetic process Source: TAIR
- carotene catabolic process Source: GO_Central
- response to water deprivation Source: TAIR
- seed dormancy process Source: TAIR
Keywordsi
Molecular function | Dioxygenase, Oxidoreductase |
Biological process | Abscisic acid biosynthesis |
Ligand | Iron, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G30100-MONOMER |
BRENDAi | 1.13.11.51, 399 |
Names & Taxonomyi
Protein namesi | Recommended name: Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic (EC:1.13.11.51)Short name: AtNCED5 |
Gene namesi | Name:NCED5 Ordered Locus Names:At1g30100 ORF Names:T2H7.10 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G30100 |
TAIRi | locus:2202492, AT1G30100 |
Subcellular locationi
Chloroplast
- chloroplast thylakoid membrane 2 Publications
Chloroplast
- chloroplast Source: GO_Central
- chloroplast stroma Source: GO_Central
- chloroplast thylakoid membrane Source: UniProtKB-SubCell
Other locations
- thylakoid Source: TAIR
Keywords - Cellular componenti
Chloroplast, Membrane, Plastid, ThylakoidPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transit peptidei | 1 – 45 | ChloroplastSequence analysisAdd BLAST | 45 | |
ChainiPRO_0000285994 | 46 – 589 | Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplasticAdd BLAST | 544 |
Proteomic databases
PaxDbi | Q9C6Z1 |
PRIDEi | Q9C6Z1 |
ProteomicsDBi | 250996 |
Expressioni
Tissue specificityi
Detected only in seeds.2 Publications
Developmental stagei
Expressed at later stages of seed maturation.1 Publication
Inductioni
Low induction by drought stress.2 Publications
Gene expression databases
ExpressionAtlasi | Q9C6Z1, baseline and differential |
Genevisiblei | Q9C6Z1, AT |
Interactioni
Subunit structurei
Interacts in vitro with VAR3.
1 PublicationProtein-protein interaction databases
IntActi | Q9C6Z1, 1 interactor |
STRINGi | 3702.AT1G30100.1 |
Family & Domainsi
Sequence similaritiesi
Belongs to the carotenoid oxygenase family.Curated
Keywords - Domaini
Transit peptidePhylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q9C6Z1 |
OMAi | FIWPFEA |
OrthoDBi | 524712at2759 |
PhylomeDBi | Q9C6Z1 |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9C6Z1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MACSYILTPN PTKLNLSFAP SDLDAPSPSS SVSFTNTKPR RRKLSANSVS
60 70 80 90 100
DTPNLLNFPN YPSPNPIIPE KDTSRWNPLQ RAASAALDFA ETALLRRERS
110 120 130 140 150
KPLPKTVDPR HQISGNYAPV PEQSVKSSLS VDGKIPDCID GVYLRNGANP
160 170 180 190 200
LFEPVSGHHL FDGDGMVHAV KITNGDASYS CRFTETERLV QEKQLGSPIF
210 220 230 240 250
PKAIGELHGH SGIARLMLFY ARGLFGLLNH KNGTGVANAG LVYFHDRLLA
260 270 280 290 300
MSEDDLPYQV RVTDNGDLET IGRFDFDGQL SSAMIAHPKI DPVTKELFAL
310 320 330 340 350
SYDVVKKPYL KYFKFSPEGE KSPDVEIPLA SPTMMHDFAI TENFVVIPDQ
360 370 380 390 400
QVVFKLSDMF LGKSPVKYDG EKISRFGILP RNAKDASEMV WVESPETFCF
410 420 430 440 450
HLWNAWESPE TDEVVVIGSC MTPADSIFNE CDEQLNSVLS EIRLNLKTGK
460 470 480 490 500
STRRTIIPGS VQMNLEAGMV NRNLLGRKTR YAYLAIAEPW PKVSGFAKVD
510 520 530 540 550
LSTGEVKNHF YGGKKYGGEP FFLPRGLESD GEDDGYIMSF VHDEESWESE
560 570 580
LHIVNAVTLE LEATVKLPSR VPYGFHGTFV NSADMLNQA
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC074176 Genomic DNA Translation: AAG50855.1 CP002684 Genomic DNA Translation: AEE31178.1 AK226774 mRNA Translation: BAE98872.1 |
PIRi | A86425 |
RefSeqi | NP_174302.1, NM_102749.3 |
Genome annotation databases
EnsemblPlantsi | AT1G30100.1; AT1G30100.1; AT1G30100 |
GeneIDi | 839889 |
Gramenei | AT1G30100.1; AT1G30100.1; AT1G30100 |
KEGGi | ath:AT1G30100 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC074176 Genomic DNA Translation: AAG50855.1 CP002684 Genomic DNA Translation: AEE31178.1 AK226774 mRNA Translation: BAE98872.1 |
PIRi | A86425 |
RefSeqi | NP_174302.1, NM_102749.3 |
3D structure databases
SMRi | Q9C6Z1 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9C6Z1, 1 interactor |
STRINGi | 3702.AT1G30100.1 |
Proteomic databases
PaxDbi | Q9C6Z1 |
PRIDEi | Q9C6Z1 |
ProteomicsDBi | 250996 |
Genome annotation databases
EnsemblPlantsi | AT1G30100.1; AT1G30100.1; AT1G30100 |
GeneIDi | 839889 |
Gramenei | AT1G30100.1; AT1G30100.1; AT1G30100 |
KEGGi | ath:AT1G30100 |
Organism-specific databases
Araporti | AT1G30100 |
TAIRi | locus:2202492, AT1G30100 |
Phylogenomic databases
eggNOGi | KOG1285, Eukaryota |
HOGENOMi | CLU_016472_0_0_1 |
InParanoidi | Q9C6Z1 |
OMAi | FIWPFEA |
OrthoDBi | 524712at2759 |
PhylomeDBi | Q9C6Z1 |
Enzyme and pathway databases
BioCyci | ARA:AT1G30100-MONOMER |
BRENDAi | 1.13.11.51, 399 |
Miscellaneous databases
PROi | PR:Q9C6Z1 |
Gene expression databases
ExpressionAtlasi | Q9C6Z1, baseline and differential |
Genevisiblei | Q9C6Z1, AT |
Family and domain databases
InterProi | View protein in InterPro IPR004294, Carotenoid_Oase |
PANTHERi | PTHR10543, PTHR10543, 1 hit |
Pfami | View protein in Pfam PF03055, RPE65, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NCED5_ARATH | |
Accessioni | Q9C6Z1Primary (citable) accession number: Q9C6Z1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2007 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 110 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families