Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

E3 ubiquitin-protein ligase SINA-like 1

Gene

At1g66610

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi114Zinc 1By similarity1
Metal bindingi186Zinc 1By similarity1
Metal bindingi198Zinc 1By similarity1
Metal bindingi202Zinc 1By similarity1
Metal bindingi209Zinc 2By similarity1
Metal bindingi214Zinc 2By similarity1
Metal bindingi226Zinc 2By similarity1
Metal bindingi231Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 92RING-type; degenerateAdd BLAST37
Zinc fingeri109 – 232SIAH-type; degeneratePROSITE-ProRule annotationAdd BLAST124

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SINA-like 1 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase SINA-like 1Curated
Seven in absentia-like protein 1
Gene namesi
Ordered Locus Names:At1g66610
ORF Names:T12I7.6
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G66610
TAIRilocus:2195170 AT1G66610

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002991901 – 366E3 ubiquitin-protein ligase SINA-like 1Add BLAST366

Proteomic databases

PaxDbiQ9C6H4
PRIDEiQ9C6H4

PTM databases

iPTMnetiQ9C6H4

Expressioni

Gene expression databases

ExpressionAtlasiQ9C6H4 differential
GenevisibleiQ9C6H4 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G66610.1

Structurei

3D structure databases

ProteinModelPortaliQ9C6H4
SMRiQ9C6H4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni106 – 354SBDBy similarityAdd BLAST249

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi355 – 362Poly-Glu8

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri56 – 92RING-type; degenerateAdd BLAST37
Zinc fingeri109 – 232SIAH-type; degeneratePROSITE-ProRule annotationAdd BLAST124

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000154324
InParanoidiQ9C6H4
OMAiNCITPCA
OrthoDBiEOG09360EP3
PhylomeDBiQ9C6H4

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 3 hits
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS51081 ZF_SIAH, 1 hit

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9C6H4-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MVKGTNAEQA LAREEASSSR PKRQRVPSIV EEEGENGGGD VVVRSGTLFE
60 70 80 90 100
LDLLDCPICC NALTIPIFQC DKGHIACSSC CTNVSNKCPY CSLAIGNYRS
110 120 130 140 150
RIMERVVEAF IVRCPIVAGE ASSSRQKRLR VPSIDEENGE NGGRDVVVPS
160 170 180 190 200
GTLSQLDLLD CPVCSKALKI SIFQQSLFLA KRQNGCTETF SYGNELVHEK
210 220 230 240 250
KCSFALCYCP APNCNYAGVY KDLYSHYAAN HKKLWTRFSC GYSMHVCMDF
260 270 280 290 300
ESKSLVLQQY SDGPLVVLQC FKEPPQGLFW TVNCIAPSAP GVGKFSYELS
310 320 330 340 350
YSTAGNTLTF RSSEMNRIQK VSFQTPEKDF MFIPEYILCP MGLYKGTYIC
360
IRSLEEEEEE EEDNED
Length:366
Mass (Da):40,788
Last modified:June 1, 2001 - v1
Checksum:i93AD2D38EF0B3A5C
GO
Isoform 2 (identifier: Q9C6H4-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     175-237: QSLFLAKRQN...AANHKKLWTR → CDNGHVACSS...DILLWQRVSP
     238-366: Missing.

Note: No experimental confirmation available.
Show »
Length:237
Mass (Da):25,965
Checksum:iC4BE133CD7C3E62D
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_027589175 – 237QSLFL…KLWTR → CDNGHVACSSCCIELRYKCP SCSLPIGNYRCIIMEKVVKA IIVPCQTPKWLHRDILLWQR VSP in isoform 2. 1 PublicationAdd BLAST63
Alternative sequenceiVSP_027590238 – 366Missing in isoform 2. 1 PublicationAdd BLAST129

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079285 Genomic DNA Translation: AAG51177.1
CP002684 Genomic DNA Translation: AEE34535.1
CP002684 Genomic DNA Translation: AEE34536.1
DQ446403 mRNA Translation: ABE65747.1
PIRiA96692
RefSeqiNP_001185331.1, NM_001198402.1 [Q9C6H4-2]
NP_176834.1, NM_105332.1 [Q9C6H4-1]
UniGeneiAt.52404

Genome annotation databases

EnsemblPlantsiAT1G66610.1; AT1G66610.1; AT1G66610 [Q9C6H4-1]
AT1G66610.2; AT1G66610.2; AT1G66610 [Q9C6H4-2]
GeneIDi842979
GrameneiAT1G66610.1; AT1G66610.1; AT1G66610 [Q9C6H4-1]
AT1G66610.2; AT1G66610.2; AT1G66610 [Q9C6H4-2]
KEGGiath:AT1G66610

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079285 Genomic DNA Translation: AAG51177.1
CP002684 Genomic DNA Translation: AEE34535.1
CP002684 Genomic DNA Translation: AEE34536.1
DQ446403 mRNA Translation: ABE65747.1
PIRiA96692
RefSeqiNP_001185331.1, NM_001198402.1 [Q9C6H4-2]
NP_176834.1, NM_105332.1 [Q9C6H4-1]
UniGeneiAt.52404

3D structure databases

ProteinModelPortaliQ9C6H4
SMRiQ9C6H4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G66610.1

PTM databases

iPTMnetiQ9C6H4

Proteomic databases

PaxDbiQ9C6H4
PRIDEiQ9C6H4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66610.1; AT1G66610.1; AT1G66610 [Q9C6H4-1]
AT1G66610.2; AT1G66610.2; AT1G66610 [Q9C6H4-2]
GeneIDi842979
GrameneiAT1G66610.1; AT1G66610.1; AT1G66610 [Q9C6H4-1]
AT1G66610.2; AT1G66610.2; AT1G66610 [Q9C6H4-2]
KEGGiath:AT1G66610

Organism-specific databases

AraportiAT1G66610
TAIRilocus:2195170 AT1G66610

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000154324
InParanoidiQ9C6H4
OMAiNCITPCA
OrthoDBiEOG09360EP3
PhylomeDBiQ9C6H4

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q9C6H4

Gene expression databases

ExpressionAtlasiQ9C6H4 differential
GenevisibleiQ9C6H4 AT

Family and domain databases

Gene3Di3.30.40.10, 2 hits
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 3 hits
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSINL1_ARATH
AccessioniPrimary (citable) accession number: Q9C6H4
Secondary accession number(s): Q1PFG0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again