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Protein

E3 ubiquitin-protein ligase SINA-like 3

Gene

At1g66630

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins. E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. It probably triggers the ubiquitin-mediated degradation of different substrates.By similarity

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi107Zinc 1By similarity1
Metal bindingi114Zinc 1By similarity1
Metal bindingi128Zinc 1By similarity1
Metal bindingi132Zinc 1By similarity1
Metal bindingi139Zinc 2By similarity1
Metal bindingi144Zinc 2By similarity1
Metal bindingi156Zinc 2By similarity1
Metal bindingi161Zinc 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 85RING-typePROSITE-ProRule annotationAdd BLAST37
Zinc fingeri102 – 162SIAH-typePROSITE-ProRule annotationAdd BLAST61

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway
LigandMetal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
E3 ubiquitin-protein ligase SINA-like 3 (EC:2.3.2.27)
Alternative name(s):
RING-type E3 ubiquitin transferase SINA-like 3Curated
Seven in absentia-like protein 3
Gene namesi
Ordered Locus Names:At1g66630
ORF Names:T12I7.8
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G66630
TAIRilocus:2195180 AT1G66630

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002991921 – 303E3 ubiquitin-protein ligase SINA-like 3Add BLAST303

Proteomic databases

PaxDbiQ9C6H2

Expressioni

Gene expression databases

ExpressionAtlasiQ9C6H2 baseline and differential
GenevisibleiQ9C6H2 AT

Interactioni

Protein-protein interaction databases

STRINGi3702.AT1G66630.1

Structurei

3D structure databases

ProteinModelPortaliQ9C6H2
SMRiQ9C6H2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni99 – 286SBDBy similarityAdd BLAST188

Domaini

The RING-type zinc finger domain is essential for ubiquitin ligase activity.By similarity
The SBD domain (substrate-binding domain) mediates the homodimerization and the interaction with substrate proteins. It is related to the TRAF family.By similarity

Sequence similaritiesi

Belongs to the SINA (Seven in absentia) family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri49 – 85RING-typePROSITE-ProRule annotationAdd BLAST37
Zinc fingeri102 – 162SIAH-typePROSITE-ProRule annotationAdd BLAST61

Keywords - Domaini

Zinc-finger

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000154324
InParanoidiQ9C6H2
KOiK04506
OMAiPENHVAN
OrthoDBiEOG09360EP3
PhylomeDBiQ9C6H2

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9C6H2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MENITNNSER SLDRPKRQRP VSMENVGGTA SGSEVARSAT LLELDLLDCP
60 70 80 90 100
ICYHKLGAPI YQCDNGHIAC SSCCKKVKYK CPYCSLRIGF FRSRILEKIV
110 120 130 140 150
EAVVVSCPNA KYGCTEKIPY DNESESAHER VCEFTLCYCP EPECKYTGVY
160 170 180 190 200
TDLYRHYHAE HKTDHSWFKC GEYNNAWLHV TGEKLSFLVL QEYEDGPLVV
210 220 230 240 250
VQCSMESHGI CVTVNCIAPC APGVGEFSCH LIYRNGSEKI TFESKKMNKI
260 270 280 290 300
QKVSPENHVA NYKPIPYYLR GEASNFMSIP YYLLDEASIL KMQICIRRSG

EEV
Length:303
Mass (Da):34,385
Last modified:June 1, 2001 - v1
Checksum:iE3B2A1F685FA29DC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079285 Genomic DNA Translation: AAG51179.1
CP002684 Genomic DNA Translation: AEE34538.1
PIRiC96692
RefSeqiNP_176836.1, NM_105334.2
UniGeneiAt.17506

Genome annotation databases

EnsemblPlantsiAT1G66630.1; AT1G66630.1; AT1G66630
GeneIDi842981
GrameneiAT1G66630.1; AT1G66630.1; AT1G66630
KEGGiath:AT1G66630

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AC079285 Genomic DNA Translation: AAG51179.1
CP002684 Genomic DNA Translation: AEE34538.1
PIRiC96692
RefSeqiNP_176836.1, NM_105334.2
UniGeneiAt.17506

3D structure databases

ProteinModelPortaliQ9C6H2
SMRiQ9C6H2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT1G66630.1

Proteomic databases

PaxDbiQ9C6H2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G66630.1; AT1G66630.1; AT1G66630
GeneIDi842981
GrameneiAT1G66630.1; AT1G66630.1; AT1G66630
KEGGiath:AT1G66630

Organism-specific databases

AraportiAT1G66630
TAIRilocus:2195180 AT1G66630

Phylogenomic databases

eggNOGiKOG3002 Eukaryota
ENOG410XVP0 LUCA
HOGENOMiHOG000154324
InParanoidiQ9C6H2
KOiK04506
OMAiPENHVAN
OrthoDBiEOG09360EP3
PhylomeDBiQ9C6H2

Enzyme and pathway databases

UniPathwayi
UPA00143

ReactomeiR-ATH-5689880 Ub-specific processing proteases
R-ATH-983168 Antigen processing: Ubiquitination & Proteasome degradation

Miscellaneous databases

PROiPR:Q9C6H2

Gene expression databases

ExpressionAtlasiQ9C6H2 baseline and differential
GenevisibleiQ9C6H2 AT

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR018121 7-in-absentia-prot_TRAF-dom
IPR004162 SINA-like
IPR008974 TRAF-like
IPR001841 Znf_RING
IPR013083 Znf_RING/FYVE/PHD
IPR013010 Znf_SIAH
PANTHERiPTHR10315 PTHR10315, 1 hit
PfamiView protein in Pfam
PF03145 Sina, 1 hit
SUPFAMiSSF49599 SSF49599, 1 hit
PROSITEiView protein in PROSITE
PS50089 ZF_RING_2, 1 hit
PS51081 ZF_SIAH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSINL3_ARATH
AccessioniPrimary (citable) accession number: Q9C6H2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 11, 2007
Last sequence update: June 1, 2001
Last modified: November 7, 2018
This is version 105 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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