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UniProtKB - Q9C5X8 (MOCOS_ARATH)
Protein
Molybdenum cofactor sulfurase
Gene
ABA3
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Sulfurates the molybdenum cofactor. Sulfation of molybdenum is essential for xanthine dehydrogenase (XDH) and aldehyde oxidase (ADO) enzymes in which molybdenum cofactor is liganded by 1 oxygen and 1 sulfur atom in active form. Modulates cold stress- and osmotic stress-responsive gene expression by acting as key regulator of abscisic acid (ABA) biosynthesis.
UniRule annotation5 PublicationsCatalytic activityi
- AH2 + L-cysteine + Mo-molybdopterin = A + H2O + L-alanine + thio-Mo-molybdopterinUniRule annotation2 PublicationsEC:2.8.1.9UniRule annotation2 Publications
Cofactori
pyridoxal 5'-phosphateUniRule annotation1 Publication
Kineticsi
- KM=50 µM for L-cysteine1 Publication
- KM=200 µM for L-selenocysteine1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 430 | 1 |
GO - Molecular functioni
- molybdenum cofactor sulfurtransferase activity Source: UniProtKB-EC
- molybdenum ion binding Source: UniProtKB-UniRule
- Mo-molybdopterin cofactor sulfurase activity Source: TAIR
- pyridoxal phosphate binding Source: UniProtKB-UniRule
- selenocysteine lyase activity Source: TAIR
GO - Biological processi
- abscisic acid biosynthetic process Source: TAIR
- auxin-activated signaling pathway Source: TAIR
- defense response to bacterium Source: TAIR
- molybdenum incorporation into molybdenum-molybdopterin complex Source: TAIR
- Mo-molybdopterin cofactor biosynthetic process Source: UniProtKB-UniRule
- protein import into chloroplast stroma Source: TAIR
- response to cold Source: TAIR
- response to glucose Source: TAIR
- response to heat Source: TAIR
- response to osmotic stress Source: TAIR
- response to salt stress Source: TAIR
- stomatal movement Source: TAIR
- sugar mediated signaling pathway Source: TAIR
Keywordsi
Molecular function | Transferase |
Biological process | Molybdenum cofactor biosynthesis |
Ligand | Pyridoxal phosphate |
Enzyme and pathway databases
BRENDAi | 2.8.1.9, 399 |
SABIO-RKi | Q9C5X8 |
Names & Taxonomyi
Protein namesi | Recommended name: Molybdenum cofactor sulfuraseUniRule annotation (EC:2.8.1.9UniRule annotation2 Publications)Short name: MCSUniRule annotation Short name: MOSUniRule annotation Short name: MoCo sulfuraseUniRule annotation Alternative name(s): Abscisic acid protein 3 Low expression of osmotically expressive genes protein 5 Molybdenum cofactor sulfurtransferaseUniRule annotation |
Gene namesi | Name:ABA3 Synonyms:LOS5 Ordered Locus Names:At1g16540 ORF Names:F19K19.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G16540 |
TAIRi | locus:2017943, AT1G16540 |
Pathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 106 | G → E in los5-1; induces a deficiency in stress-induced ABA accumulation. 1 Publication | 1 | |
Mutagenesisi | 271 | K → S: Loss of function. 1 Publication | 1 | |
Mutagenesisi | 430 | C → A: Induces a strong reduction in enzyme activity. 1 Publication | 1 | |
Mutagenesisi | 469 | G → E in aba3-1; induces a reduced ABA biosynthesis. 2 Publications | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000249958 | 1 – 819 | Molybdenum cofactor sulfuraseAdd BLAST | 819 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 271 | N6-(pyridoxal phosphate)lysine | 1 |
Proteomic databases
PaxDbi | Q9C5X8 |
PRIDEi | Q9C5X8 |
ProteomicsDBi | 238297 |
PTM databases
iPTMneti | Q9C5X8 |
Expressioni
Tissue specificityi
Ubiquitously expressed.1 Publication
Inductioni
Up-regulated in response to drought, salt or ABA treatment.1 Publication
Gene expression databases
ExpressionAtlasi | Q9C5X8, baseline and differential |
Genevisiblei | Q9C5X8, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 650 – 817 | MOSCUniRule annotationAdd BLAST | 168 |
Sequence similaritiesi
Belongs to the class-V pyridoxal-phosphate-dependent aminotransferase family. MOCOS subfamily.UniRule annotation
Phylogenomic databases
eggNOGi | KOG2142, Eukaryota |
HOGENOMi | CLU_010913_0_1_1 |
InParanoidi | Q9C5X8 |
PhylomeDBi | Q9C5X8 |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_03050, MOCOS, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR005302, MoCF_Sase_C IPR028886, MoCo_sulfurase IPR005303, MOSC_N IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small IPR011037, Pyrv_Knase-like_insert_dom_sf |
Pfami | View protein in Pfam PF00266, Aminotran_5, 2 hits PF03473, MOSC, 1 hit PF03476, MOSC_N, 1 hit |
SUPFAMi | SSF50800, SSF50800, 1 hit SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS51340, MOSC, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9C5X8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEAFLKEFGD YYGYPDGPKN IQEIRDTEFK RLDKGVVYLD HAGSTLYSEL
60 70 80 90 100
QMEYIFKDFT SNVFGNPHSQ SDISSATSDL IADARHQVLE YFNASPEDYS
110 120 130 140 150
CLFTSGATAA LKLVGETFPW TQDSNFLYTM ENHNSVLGIR EYALAQGASA
160 170 180 190 200
CAVDIEEAAN QPGQLTNSGP SIKVKHRAVQ MRNTSKLQKE ESRGNAYNLF
210 220 230 240 250
AFPSECNFSG LRFNLDLVKL MKENTETVLQ GSPFSKSKRW MVLIDAAKGC
260 270 280 290 300
ATLPPDLSEY PADFVVLSFY KLFGYPTGLG ALLVRNDAAK LLKKTYFSGG
310 320 330 340 350
TVAASIADID FVKRRERVEE FFEDGSASFL SIAAIRHGFK LLKSLTPSAI
360 370 380 390 400
WMHTTSLSIY VKKKLQALRH GNGAAVCVLY GSENLELSSH KSGPTVTFNL
410 420 430 440 450
KRPDGSWFGY LEVEKLASLS GIQLRTGCFC NPGACAKYLE LSHSELRSNV
460 470 480 490 500
EAGHICWDDN DVINGKPTGA VRVSFGYMST FEDAKKFIDF IISSFASPPK
510 520 530 540 550
KTGNGTVVSG RFPQLPSEDL ESKESFPSHY LKSITVYPIK SCAGFSVIRW
560 570 580 590 600
PLCRTGLLHD REWMVQGLTG EILTQKKVPE MSLIKTFIDL EEGLLSVESS
610 620 630 640 650
RCEDKLHIRI KSDSYNPRND EFDSHANILE NRNEETRINR WFTNAIGRQC
660 670 680 690 700
KLLRYSSSTS KDCLNRNKSP GLCRDLESNI NFANEAQFLL ISEESVADLN
710 720 730 740 750
RRLEAKDEDY KRAHEKLNPH RFRPNLVISG GEPYGEDKWK TVKIGDNHFT
760 770 780 790 800
SLGGCNRCQM INISNEAGLV KKSNEPLTTL ASYRRVKGKI LFGTLLRYEI
810
DEKRQCWIGV GEEVNPDIE
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1P8AVG9 | A0A1P8AVG9_ARATH | Molybdenum cofactor sulfurase | ABA3 ABA DEFICIENT 3, ACI2, ALTERED CHLOROPLAST IMPORT 2, ATABA3, AtLOS5 | 842 | Annotation score: | ||
A0A1P8AVE2 | A0A1P8AVE2_ARATH | Molybdenum cofactor sulfurase | ABA3 ABA DEFICIENT 3, ACI2, ALTERED CHLOROPLAST IMPORT 2, ATABA3, AtLOS5 | 724 | Annotation score: |
Sequence cautioni
The sequence AAG10824 differs from that shown. Reason: Erroneous gene model prediction.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF325457 mRNA Translation: AAK12939.1 AY034895 mRNA Translation: AAK58888.1 AC011808 Genomic DNA Translation: AAG10824.1 Sequence problems. CP002684 Genomic DNA Translation: AEE29467.1 |
PIRi | G86300 |
RefSeqi | NP_564001.1, NM_101519.3 |
Genome annotation databases
EnsemblPlantsi | AT1G16540.1; AT1G16540.1; AT1G16540 |
GeneIDi | 838224 |
Gramenei | AT1G16540.1; AT1G16540.1; AT1G16540 |
KEGGi | ath:AT1G16540 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF325457 mRNA Translation: AAK12939.1 AY034895 mRNA Translation: AAK58888.1 AC011808 Genomic DNA Translation: AAG10824.1 Sequence problems. CP002684 Genomic DNA Translation: AEE29467.1 |
PIRi | G86300 |
RefSeqi | NP_564001.1, NM_101519.3 |
3D structure databases
SMRi | Q9C5X8 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT1G16540.1 |
PTM databases
iPTMneti | Q9C5X8 |
Proteomic databases
PaxDbi | Q9C5X8 |
PRIDEi | Q9C5X8 |
ProteomicsDBi | 238297 |
Genome annotation databases
EnsemblPlantsi | AT1G16540.1; AT1G16540.1; AT1G16540 |
GeneIDi | 838224 |
Gramenei | AT1G16540.1; AT1G16540.1; AT1G16540 |
KEGGi | ath:AT1G16540 |
Organism-specific databases
Araporti | AT1G16540 |
TAIRi | locus:2017943, AT1G16540 |
Phylogenomic databases
eggNOGi | KOG2142, Eukaryota |
HOGENOMi | CLU_010913_0_1_1 |
InParanoidi | Q9C5X8 |
PhylomeDBi | Q9C5X8 |
Enzyme and pathway databases
BRENDAi | 2.8.1.9, 399 |
SABIO-RKi | Q9C5X8 |
Miscellaneous databases
PROi | PR:Q9C5X8 |
Gene expression databases
ExpressionAtlasi | Q9C5X8, baseline and differential |
Genevisiblei | Q9C5X8, AT |
Family and domain databases
Gene3Di | 3.40.640.10, 1 hit 3.90.1150.10, 1 hit |
HAMAPi | MF_03050, MOCOS, 1 hit |
InterProi | View protein in InterPro IPR000192, Aminotrans_V_dom IPR005302, MoCF_Sase_C IPR028886, MoCo_sulfurase IPR005303, MOSC_N IPR015424, PyrdxlP-dep_Trfase IPR015421, PyrdxlP-dep_Trfase_major IPR015422, PyrdxlP-dep_Trfase_small IPR011037, Pyrv_Knase-like_insert_dom_sf |
Pfami | View protein in Pfam PF00266, Aminotran_5, 2 hits PF03473, MOSC, 1 hit PF03476, MOSC_N, 1 hit |
SUPFAMi | SSF50800, SSF50800, 1 hit SSF53383, SSF53383, 1 hit |
PROSITEi | View protein in PROSITE PS51340, MOSC, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MOCOS_ARATH | |
Accessioni | Q9C5X8Primary (citable) accession number: Q9C5X8 Secondary accession number(s): Q9FX72 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2006 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families