UniProtKB - Q9C5Q8 (HEN1_ARATH)
Protein
Small RNA 2'-O-methyltransferase
Gene
HEN1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Methyltransferase that adds a methyl group to the ribose of the last nucleotide of small RNAs (sRNAs). This protects the 3'-end of sRNAs from uridylation activity and subsequent degradation. Can methylate 3'-end of microRNAs (miRNAs), small interfering RNAs (siRNas) and trans-acting small interfering RNAs (ta-siRNAs). Involved in plant development through its role in small RNAs processing. Required for the specification of reproductive organ identities and the probable repression of A class genes. May control floral determinacy possibly by regulating the expression of the C class floral homeotic gene AGAMOUS (AG).4 Publications
Catalytic activityi
- S-adenosyl-L-methionine + small RNA 3'-end nucleotide = H+ + S-adenosyl-L-homocysteine + small RNA 3'-end 2'-O-methylnucleotideEC:2.1.1.n8
Cofactori
Mg2+1 PublicationNote: Binds 1 Mg2+ ion per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 726 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 745 | S-adenosyl-L-methionineCombined sources1 Publication | 1 | |
Binding sitei | 795 | S-adenosyl-L-methionine; via carbonyl oxygenCombined sources1 Publication | 1 | |
Metal bindingi | 796 | MagnesiumCombined sources1 Publication | 1 | |
Metal bindingi | 799 | MagnesiumCombined sources1 Publication | 1 | |
Metal bindingi | 800 | Magnesium; via tele nitrogenCombined sources1 Publication | 1 | |
Metal bindingi | 860 | Magnesium; via tele nitrogenCombined sources1 Publication | 1 |
GO - Molecular functioni
- metal ion binding Source: UniProtKB-KW
- O-methyltransferase activity Source: GO_Central
- RNA binding Source: UniProtKB-KW
- RNA methyltransferase activity Source: TAIR
GO - Biological processi
- leaf proximal/distal pattern formation Source: TAIR
- leaf vascular tissue pattern formation Source: TAIR
- miRNA 2'-O-methylation Source: CACAO
- mRNA cleavage involved in gene silencing by miRNA Source: TAIR
- piRNA metabolic process Source: GO_Central
- production of miRNAs involved in gene silencing by miRNA Source: TAIR
- production of siRNA involved in RNA interference Source: GO_Central
- regulation of flower development Source: TAIR
- RNAi-mediated antiviral immune response Source: TAIR
- specification of floral organ identity Source: TAIR
Keywordsi
Molecular function | Methyltransferase, RNA-binding, Transferase |
Biological process | RNA-mediated gene silencing |
Ligand | Magnesium, Metal-binding, S-adenosyl-L-methionine |
Names & Taxonomyi
Protein namesi | Recommended name: Small RNA 2'-O-methyltransferase (EC:2.1.1.n8)Alternative name(s): Protein CORYMBOSA 2 Protein HUA ENHANCER 1 S-adenosylmethionine-dependent RNA methyltransferase HEN1 |
Gene namesi | Name:HEN1 Synonyms:CRM2 Ordered Locus Names:At4g20910 ORF Names:T13K14.70 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT4G20910 |
TAIRi | locus:2133114, AT4G20910 |
Pathology & Biotechi
Disruption phenotypei
Reduced organ size, altered rosette leaf shape and increased number of coflorescences. Urydilation of miRNA 3'-ends.3 Publications
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 68 | Missing in crm2-1; compact inflorescence with several flower buds at the tip. 1 Publication | 1 | |
Mutagenesisi | 109 | Y → A: No effect on RNA binding and transferase activity. 1 Publication | 1 | |
Mutagenesisi | 333 | W → A: Loss of RNA binding and transferase activity. 1 Publication | 1 | |
Mutagenesisi | 604 | L → P: No effect on transferase activity. 1 Publication | 1 | |
Mutagenesisi | 701 | R → A: Reduces transferase activity. 1 Publication | 1 | |
Mutagenesisi | 719 | D → N in hen1-2; alters organ development and floral determinacy. 1 Publication | 1 | |
Mutagenesisi | 796 | E → A: Loss of transferase activity. 1 Publication | 1 | |
Mutagenesisi | 799 | E → A: Loss of transferase activity; when associated with A-800. 1 Publication | 1 | |
Mutagenesisi | 800 | H → A: Loss of transferase activity; when associated with A-799. 1 Publication | 1 | |
Mutagenesisi | 800 | H → Q: Strongly decreases transferase activity. 1 Publication | 1 | |
Mutagenesisi | 856 | R → A: Reduces transferase activity. 1 Publication | 1 | |
Mutagenesisi | 860 | H → A or Q: Loss of transferase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000404658 | 1 – 942 | Small RNA 2'-O-methyltransferaseAdd BLAST | 942 |
Proteomic databases
PaxDbi | Q9C5Q8 |
PRIDEi | Q9C5Q8 |
ProteomicsDBi | 230384 |
PTM databases
iPTMneti | Q9C5Q8 |
Expressioni
Tissue specificityi
Expressed in stems, leaves and inflorescences.2 Publications
Gene expression databases
ExpressionAtlasi | Q9C5Q8, baseline and differential |
Genevisiblei | Q9C5Q8, AT |
Interactioni
Subunit structurei
Binds small RNA duplexes as monomer.
1 PublicationProtein-protein interaction databases
BioGRIDi | 13130, 3 interactors |
STRINGi | 3702.AT4G20910.2 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9C5Q8 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9C5Q8 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 87 | DRBMAdd BLAST | 69 | |
Domaini | 93 – 204 | HTH La-type RNA-bindingPROSITE-ProRule annotationAdd BLAST | 112 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 778 – 779 | S-adenosyl-L-methionine bindingCombined sources1 Publication | 2 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1045, Eukaryota |
HOGENOMi | CLU_001342_0_0_1 |
OMAi | ACYSVEE |
OrthoDBi | 189384at2759 |
PhylomeDBi | Q9C5Q8 |
Family and domain databases
InterProi | View protein in InterPro IPR040870, HEN1_dsRBD2 IPR040813, Hen1_Lam_C IPR006630, La_HTH IPR025714, Methyltranfer_dom IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF17842, dsRBD2, 1 hit PF18441, Hen1_Lam_C, 1 hit PF13847, Methyltransf_31, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS50961, HTH_LA, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9C5Q8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAGGGKHTPT PKAIIHQKFG AKASYTVEEV HDSSQSGCLG LAIPQKGPCL
60 70 80 90 100
YRCHLQLPEF SVVSNVFKKK KDSEQSAAEL ALDKLGIRPQ NDDLTVDEAR
110 120 130 140 150
DEIVGRIKYI FSDEFLSAEH PLGAHLRAAL RRDGERCGSV PVSVIATVDA
160 170 180 190 200
KINSRCKIIN PSVESDPFLA ISYVMKAAAK LADYIVASPH GLRRKNAYPS
210 220 230 240 250
EIVEALATHV SDSLHSREVA AVYIPCIDEE VVELDTLYIS SNRHYLDSIA
260 270 280 290 300
ERLGLKDGNQ VMISRMFGKA SCGSECRLYS EIPKKYLDNS SDASGTSNED
310 320 330 340 350
SSHIVKSRNA RASYICGQDI HGDAILASVG YRWKSDDLDY DDVTVNSFYR
360 370 380 390 400
ICCGMSPNGI YKISRQAVIA AQLPFAFTTK SNWRGPLPRE ILGLFCHQHR
410 420 430 440 450
LAEPILSSST APVKSLSDIF RSHKKLKVSG VDDANENLSR QKEDTPGLGH
460 470 480 490 500
GFRCEVKIFT KSQDLVLECS PRKFYEKEND AIQNASLKAL LWFSKFFADL
510 520 530 540 550
DVDGEQSCDT DDDQDTKSSS PNVFAAPPIL QKEHSSESKN TNVLSAEKRV
560 570 580 590 600
QSITNGSVVS ICYSLSLAVD PEYSSDGESP REDNESNEEM ESEYSANCES
610 620 630 640 650
SVELIESNEE IEFEVGTGSM NPHIESEVTQ MTVGEYASFR MTPPDAAEAL
660 670 680 690 700
ILAVGSDTVR IRSLLSERPC LNYNILLLGV KGPSEERMEA AFFKPPLSKQ
710 720 730 740 750
RVEYALKHIR ESSASTLVDF GCGSGSLLDS LLDYPTSLQT IIGVDISPKG
760 770 780 790 800
LARAAKMLHV KLNKEACNVK SATLYDGSIL EFDSRLHDVD IGTCLEVIEH
810 820 830 840 850
MEEDQACEFG EKVLSLFHPK LLIVSTPNYE FNTILQRSTP ETQEENNSEP
860 870 880 890 900
QLPKFRNHDH KFEWTREQFN QWASKLGKRH NYSVEFSGVG GSGEVEPGFA
910 920 930 940
SQIAIFRREA SSVENVAESS MQPYKVIWEW KKEDVEKKKT DL
Sequence cautioni
The sequence CAB45886 differs from that shown. Reason: Erroneous gene model prediction.Curated
The sequence CAB79091 differs from that shown. Reason: Erroneous gene model prediction.Curated
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 39 | L → P in AAC97105 (Ref. 1) Curated | 1 | |
Sequence conflicti | 39 | L → P in AAL05056 (PubMed:11874905).Curated | 1 | |
Sequence conflicti | 552 | S → Y in AAC97105 (Ref. 1) Curated | 1 | |
Sequence conflicti | 660 | R → K in AAC97105 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060248 Genomic DNA Translation: AAC97105.1 AF411383 mRNA Translation: AAL05056.1 AF327068 mRNA Translation: AAK16435.1 AL080282 Genomic DNA Translation: CAB45886.1 Sequence problems. AL161553 Genomic DNA Translation: CAB79091.1 Sequence problems. CP002687 Genomic DNA Translation: AEE84374.1 CP002687 Genomic DNA Translation: AEE84375.1 |
PIRi | T10633 |
RefSeqi | NP_001190782.1, NM_001203853.2 NP_567616.1, NM_118209.3 |
Genome annotation databases
EnsemblPlantsi | AT4G20910.1; AT4G20910.1; AT4G20910 AT4G20910.2; AT4G20910.2; AT4G20910 |
GeneIDi | 827839 |
Gramenei | AT4G20910.1; AT4G20910.1; AT4G20910 AT4G20910.2; AT4G20910.2; AT4G20910 |
KEGGi | ath:AT4G20910 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF060248 Genomic DNA Translation: AAC97105.1 AF411383 mRNA Translation: AAL05056.1 AF327068 mRNA Translation: AAK16435.1 AL080282 Genomic DNA Translation: CAB45886.1 Sequence problems. AL161553 Genomic DNA Translation: CAB79091.1 Sequence problems. CP002687 Genomic DNA Translation: AEE84374.1 CP002687 Genomic DNA Translation: AEE84375.1 |
PIRi | T10633 |
RefSeqi | NP_001190782.1, NM_001203853.2 NP_567616.1, NM_118209.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
3HTX | X-ray | 3.10 | A/D | 1-942 | [»] | |
SMRi | Q9C5Q8 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 13130, 3 interactors |
STRINGi | 3702.AT4G20910.2 |
PTM databases
iPTMneti | Q9C5Q8 |
Proteomic databases
PaxDbi | Q9C5Q8 |
PRIDEi | Q9C5Q8 |
ProteomicsDBi | 230384 |
Genome annotation databases
EnsemblPlantsi | AT4G20910.1; AT4G20910.1; AT4G20910 AT4G20910.2; AT4G20910.2; AT4G20910 |
GeneIDi | 827839 |
Gramenei | AT4G20910.1; AT4G20910.1; AT4G20910 AT4G20910.2; AT4G20910.2; AT4G20910 |
KEGGi | ath:AT4G20910 |
Organism-specific databases
Araporti | AT4G20910 |
TAIRi | locus:2133114, AT4G20910 |
Phylogenomic databases
eggNOGi | KOG1045, Eukaryota |
HOGENOMi | CLU_001342_0_0_1 |
OMAi | ACYSVEE |
OrthoDBi | 189384at2759 |
PhylomeDBi | Q9C5Q8 |
Miscellaneous databases
EvolutionaryTracei | Q9C5Q8 |
PROi | PR:Q9C5Q8 |
Gene expression databases
ExpressionAtlasi | Q9C5Q8, baseline and differential |
Genevisiblei | Q9C5Q8, AT |
Family and domain databases
InterProi | View protein in InterPro IPR040870, HEN1_dsRBD2 IPR040813, Hen1_Lam_C IPR006630, La_HTH IPR025714, Methyltranfer_dom IPR029063, SAM-dependent_MTases |
Pfami | View protein in Pfam PF17842, dsRBD2, 1 hit PF18441, Hen1_Lam_C, 1 hit PF13847, Methyltransf_31, 1 hit |
SUPFAMi | SSF53335, SSF53335, 1 hit |
PROSITEi | View protein in PROSITE PS50961, HTH_LA, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | HEN1_ARATH | |
Accessioni | Q9C5Q8Primary (citable) accession number: Q9C5Q8 Secondary accession number(s): O65309, Q945R3, Q9SUC2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 8, 2011 |
Last sequence update: | June 1, 2001 | |
Last modified: | April 7, 2021 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families