UniProtKB - Q9C5P0 (SUVH8_ARATH)
Protein
Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH8
Gene
SUVH8
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Histone methyltransferase. Methylates 'Lys-9' of histone H3. H3 'Lys-9' methylation represents a specific tag for epigenetic transcriptional repression.
Catalytic activityi
- L-lysyl9-[histone H3] + S-adenosyl-L-methionine = H+ + N6-methyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteinePROSITE-ProRule annotationCurated
- N6-methyl-L-lysyl9-[histone H3] + S-adenosyl-L-methionine = H+ + N6,N6-dimethyl-L-lysyl9-[histone H3] + S-adenosyl-L-homocysteinePROSITE-ProRule annotationCurated
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 624 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 626 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Binding sitei | 676 | S-adenosyl-L-methioninePROSITE-ProRule annotation | 1 | |
Metal bindingi | 682 | ZincBy similarity | 1 | |
Metal bindingi | 743 | ZincBy similarity | 1 | |
Metal bindingi | 745 | ZincBy similarity | 1 | |
Metal bindingi | 750 | ZincBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 174 – 186 | A.T hookAdd BLAST | 13 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- histone-lysine N-methyltransferase activity Source: InterPro
- histone methyltransferase activity Source: TAIR
- zinc ion binding Source: InterPro
GO - Biological processi
- leaf development Source: TAIR
- regulation of cell size Source: TAIR
- regulation of gene expression, epigenetic Source: TAIR
Keywordsi
Molecular function | Chromatin regulator, DNA-binding, Methyltransferase, Transferase |
Ligand | Metal-binding, S-adenosyl-L-methionine, Zinc |
Names & Taxonomyi
Protein namesi | Recommended name: Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH8 (EC:2.1.1.-Curated)Alternative name(s): Histone H3-K9 methyltransferase 8 Short name: H3-K9-HMTase 8 Protein SET DOMAIN GROUP 21 Suppressor of variegation 3-9 homolog protein 8 Short name: Su(var)3-9 homolog protein 8 |
Gene namesi | Name:SUVH8 Synonyms:SDG21, SET21 Ordered Locus Names:At2g24740 ORF Names:F27A10.5 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G24740 |
TAIRi | locus:2047266, AT2G24740 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Other locations
- centromere By similarity
Note: Associates with centromeric constitutive heterochromatin.By similarity
Nucleus
- nucleus Source: UniProtKB-SubCell
Other locations
- chromosome, centromeric region Source: UniProtKB-SubCell
Keywords - Cellular componenti
Centromere, Chromosome, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000186079 | 1 – 755 | Histone-lysine N-methyltransferase, H3 lysine-9 specific SUVH8Add BLAST | 755 |
Proteomic databases
PaxDbi | Q9C5P0 |
PRIDEi | Q9C5P0 |
ProteomicsDBi | 226524 |
PTM databases
iPTMneti | Q9C5P0 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9C5P0, baseline and differential |
Genevisiblei | Q9C5P0, AT |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 310 – 448 | YDGPROSITE-ProRule annotationAdd BLAST | 139 | |
Domaini | 528 – 578 | Pre-SETAdd BLAST | 51 | |
Domaini | 581 – 723 | SETPROSITE-ProRule annotationAdd BLAST | 143 | |
Domaini | 739 – 755 | Post-SETPROSITE-ProRule annotationAdd BLAST | 17 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 591 – 593 | S-adenosyl-L-methionine bindingBy similarity | 3 | |
Regioni | 679 – 680 | S-adenosyl-L-methionine bindingBy similarity | 2 |
Domaini
Although the SET domain contains the active site of enzymatic activity, both pre-SET and post-SET domains are required for methyltransferase activity.
Cys residues in the pre-SET domain normally bind 3 zinc ions that are arranged in a triangular cluster, but here these Cys residues are only partially conserved, suggesting that the pre-Set domain may not bind a zinc cluster.
Sequence similaritiesi
Belongs to the class V-like SAM-binding methyltransferase superfamily. Histone-lysine methyltransferase family. Suvar3-9 subfamily.PROSITE-ProRule annotation
Phylogenomic databases
eggNOGi | KOG1082, Eukaryota |
HOGENOMi | CLU_004556_2_1_1 |
InParanoidi | Q9C5P0 |
OMAi | MTRRIGP |
OrthoDBi | 197626at2759 |
PhylomeDBi | Q9C5P0 |
Family and domain databases
Gene3Di | 2.30.280.10, 1 hit |
InterProi | View protein in InterPro IPR025794, Hist-Lys_N-MeTrfase_plant IPR003616, Post-SET_dom IPR007728, Pre-SET_dom IPR015947, PUA-like_sf IPR001214, SET_dom IPR036987, SRA-YDG_sf IPR003105, SRA_YDG |
Pfami | View protein in Pfam PF05033, Pre-SET, 1 hit PF02182, SAD_SRA, 1 hit PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM00468, PreSET, 1 hit SM00317, SET, 1 hit SM00466, SRA, 1 hit |
SUPFAMi | SSF88697, SSF88697, 1 hit |
PROSITEi | View protein in PROSITE PS50868, POST_SET, 1 hit PS51575, SAM_MT43_SUVAR39_2, 1 hit PS50280, SET, 1 hit PS51015, YDG, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9C5P0-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MVSTPPTLLM LFDDGDAGPS TGLVHREKSD AVNEEAHATS VPPHAPPQTL
60 70 80 90 100
WLLDNFNIED SYDRDAGPST GPVHRERSDA VNEEAHATSI PPHAPPQTLW
110 120 130 140 150
LLDNFNIEDS YDRDAGPSTS PIDREASHEV NEDAHATSAP PHVMVSPLQN
160 170 180 190 200
RRPFDQFNNQ PYDASAGPST GPGKRGRGRP KGSKNGSRKP KKPKAYDNNS
210 220 230 240 250
TDASAGPSSG LGKRRCGRPK GLKNRSRKPK KPKADDPNSK MVISCPDFDS
260 270 280 290 300
RITEAERESG NQEIVDSILM RFDAVRRRLC QLNYRKDKIL TASTNCMNLG
310 320 330 340 350
VRTNMTRRIG PIPGVQVGDI FYYWCEMCLV GLHRNTAGGI DSLLAKESGV
360 370 380 390 400
DGPAATSVVT SGKYDNETED LETLIYSGHG GKPCDQVLQR GNRALEASVR
410 420 430 440 450
RRNEVRVIRG ELYNNEKVYI YDGLYLVSDC WQVTGKSGFK EYRFKLLRKP
460 470 480 490 500
GQPPGYAIWK LVENLRNHEL IDPRQGFILG DLSFGEEGLR VPLVNEVDEE
510 520 530 540 550
DKTIPDDFDY IRSQCYSGMT NDVNVDSQSL VQSYIHQNCT CILKNCGQLP
560 570 580 590 600
YHDNILVCRK PLIYECGGSC PTRMVETGLK LHLEVFKTSN CGWGLRSWDP
610 620 630 640 650
IRAGTFICEF TGVSKTKEEV EEDDDYLFDT SRIYHSFRWN YEPELLCEDA
660 670 680 690 700
CEQVSEDANL PTQVLISAKE KGNVGRFMNH NCWPNVFWQP IEYDDNNGHI
710 720 730 740 750
YVRIGLFAMK HIPPMTELTY DYGISCVEKT GEDEVIYKGK KICLCGSVKC
RGSFG
Sequence cautioni
The sequence AAD26896 differs from that shown. Reason: Erroneous initiation.Curated
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF344451 Genomic DNA Translation: AAK28973.1 AC007266 Genomic DNA Translation: AAD26896.1 Different initiation. CP002685 Genomic DNA Translation: AEC07623.1 |
PIRi | C84640 |
RefSeqi | NP_180049.2, NM_128034.2 |
Genome annotation databases
EnsemblPlantsi | AT2G24740.1; AT2G24740.1; AT2G24740 |
GeneIDi | 817010 |
Gramenei | AT2G24740.1; AT2G24740.1; AT2G24740 |
KEGGi | ath:AT2G24740 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF344451 Genomic DNA Translation: AAK28973.1 AC007266 Genomic DNA Translation: AAD26896.1 Different initiation. CP002685 Genomic DNA Translation: AEC07623.1 |
PIRi | C84640 |
RefSeqi | NP_180049.2, NM_128034.2 |
3D structure databases
SMRi | Q9C5P0 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 3702.AT2G24740.1 |
PTM databases
iPTMneti | Q9C5P0 |
Proteomic databases
PaxDbi | Q9C5P0 |
PRIDEi | Q9C5P0 |
ProteomicsDBi | 226524 |
Genome annotation databases
EnsemblPlantsi | AT2G24740.1; AT2G24740.1; AT2G24740 |
GeneIDi | 817010 |
Gramenei | AT2G24740.1; AT2G24740.1; AT2G24740 |
KEGGi | ath:AT2G24740 |
Organism-specific databases
Araporti | AT2G24740 |
TAIRi | locus:2047266, AT2G24740 |
Phylogenomic databases
eggNOGi | KOG1082, Eukaryota |
HOGENOMi | CLU_004556_2_1_1 |
InParanoidi | Q9C5P0 |
OMAi | MTRRIGP |
OrthoDBi | 197626at2759 |
PhylomeDBi | Q9C5P0 |
Miscellaneous databases
PROi | PR:Q9C5P0 |
Gene expression databases
ExpressionAtlasi | Q9C5P0, baseline and differential |
Genevisiblei | Q9C5P0, AT |
Family and domain databases
Gene3Di | 2.30.280.10, 1 hit |
InterProi | View protein in InterPro IPR025794, Hist-Lys_N-MeTrfase_plant IPR003616, Post-SET_dom IPR007728, Pre-SET_dom IPR015947, PUA-like_sf IPR001214, SET_dom IPR036987, SRA-YDG_sf IPR003105, SRA_YDG |
Pfami | View protein in Pfam PF05033, Pre-SET, 1 hit PF02182, SAD_SRA, 1 hit PF00856, SET, 1 hit |
SMARTi | View protein in SMART SM00468, PreSET, 1 hit SM00317, SET, 1 hit SM00466, SRA, 1 hit |
SUPFAMi | SSF88697, SSF88697, 1 hit |
PROSITEi | View protein in PROSITE PS50868, POST_SET, 1 hit PS51575, SAM_MT43_SUVAR39_2, 1 hit PS50280, SET, 1 hit PS51015, YDG, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | SUVH8_ARATH | |
Accessioni | Q9C5P0Primary (citable) accession number: Q9C5P0 Secondary accession number(s): Q9TP24 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 9, 2003 |
Last sequence update: | June 1, 2001 | |
Last modified: | October 7, 2020 | |
This is version 139 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families