UniProtKB - Q9C5E5 (DGK5_ARATH)
Protein
Diacylglycerol kinase 5
Gene
DGK5
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Phosphorylates the second messenger diacylglycerol (DAG) to generate phosphatidic acid (PA), another important signaling molecule. PA is required for plant development and responses to abiotic stress and pathogen attack. May be involved in the accumulation of PA during cold stress (By similarity).By similarity
Catalytic activityi
- EC:2.7.1.107
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- diacylglycerol kinase activity Source: UniProtKB-EC
- NAD+ kinase activity Source: InterPro
GO - Biological processi
- defense response Source: UniProtKB-KW
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: InterPro
Keywordsi
Molecular function | Kinase, Transferase |
Biological process | Plant defense, Stress response |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BioCyci | ARA:AT2G20900-MONOMER |
Names & Taxonomyi
Protein namesi | Recommended name: Diacylglycerol kinase 5 (EC:2.7.1.107)Short name: AtDGK5 Short name: DAG kinase 5 Alternative name(s): Diglyceride kinase 5 Short name: DGK 5 |
Gene namesi | Name:DGK5 Ordered Locus Names:At2g20900 ORF Names:F5H14.13 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT2G20900 |
TAIRi | locus:2051343, AT2G20900 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000422113 | 1 – 509 | Diacylglycerol kinase 5Add BLAST | 509 |
Proteomic databases
PaxDbi | Q9C5E5 |
PRIDEi | Q9C5E5 |
ProteomicsDBi | 224215 [Q9C5E5-1] |
PTM databases
iPTMneti | Q9C5E5 |
Expressioni
Gene expression databases
ExpressionAtlasi | Q9C5E5, baseline and differential |
Genevisiblei | Q9C5E5, AT |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
BioGRIDi | 1977, 1 interactor |
IntActi | Q9C5E5, 1 interactor |
STRINGi | 3702.AT2G20900.1 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 36 – 187 | DAGKcPROSITE-ProRule annotationAdd BLAST | 152 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 24 – 32 | Poly-Glu | 9 |
Sequence similaritiesi
Belongs to the eukaryotic diacylglycerol kinase family.Curated
Phylogenomic databases
eggNOGi | KOG1169, Eukaryota |
InParanoidi | Q9C5E5 |
OMAi | IDSWHIM |
OrthoDBi | 633642at2759 |
PhylomeDBi | Q9C5E5 |
Family and domain databases
Gene3Di | 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR037607, DGK IPR000756, Diacylglycerol_kin_accessory IPR001206, Diacylglycerol_kinase_cat_dom IPR016961, Diacylglycerol_kinase_pln IPR016064, NAD/diacylglycerol_kinase_sf |
PANTHERi | PTHR11255, PTHR11255, 1 hit |
Pfami | View protein in Pfam PF00609, DAGK_acc, 1 hit PF00781, DAGK_cat, 1 hit |
PIRSFi | PIRSF030829, Diacylglycerol_kinase_pln, 1 hit |
SMARTi | View protein in SMART SM00045, DAGKa, 1 hit SM00046, DAGKc, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9C5E5-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MEKYNSLSDF LKEFYIPTYV LSAETEEEEE EESRPTPASP VLVFINSKSG
60 70 80 90 100
GQLGGELILT YRSLLNHNQV FDLDQETPDK VLRRIYLNLE RLKDDDFARQ
110 120 130 140 150
IREKLKIIVA GGDGTAGWLL GVVCDLKLSH PPPIATVPLG TGNNLPFAFG
160 170 180 190 200
WGKKNPGTDR TAVESFLEQV LKAKVMKIDN WHILMRMKTP KEGGSCDPVA
210 220 230 240 250
PLELPHSLHA FHRVSPTDEL NKEGCHTFRG GFWNYFSLGM DAQISYAFHS
260 270 280 290 300
ERKLHPEKFK NQLVNQSTYV KLGCTQGWFC ASLFHPASRN IAQLAKVKIA
310 320 330 340 350
TRNGQWQDLH IPHSIRSIVC LNLPSFSGGL NPWGTPNPRK QRDRGLTPPF
360 370 380 390 400
VDDGLIEVVG FRNAWHGLVL LAPNGHGTRL AQANRIRFEF HKGATDHTFM
410 420 430 440 450
RMDGEPWKQP LPLDDETVMV EISHLGQVNM LATHDCRSRS VFDPSTPRHQ
460 470 480 490 500
DGAEDYDDNE DDSVAEGEEF RKFGAADTFK IPDEGEHSNK KGRASRRRNS
NVHGWSHVL
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 31 | E → Q in AAM62810 (Ref. 5) Curated | 1 | |
Sequence conflicti | 97 | F → S in AAM62810 (Ref. 5) Curated | 1 | |
Sequence conflicti | 102 | R → G in AAM62810 (Ref. 5) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_046410 | 485 – 491 | GEHSNKK → VDISQLS in isoform 2. 2 Publications | 7 | |
Alternative sequenceiVSP_046411 | 492 – 509 | Missing in isoform 2. 2 PublicationsAdd BLAST | 18 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC006234 Genomic DNA Translation: AAD20931.2 CP002685 Genomic DNA Translation: AEC07093.1 CP002685 Genomic DNA Translation: AEC07094.1 CP002685 Genomic DNA Translation: AEC07095.1 CP002685 Genomic DNA Translation: AEC07096.1 AF360300 mRNA Translation: AAK26010.1 BT000983 mRNA Translation: AAN41383.1 AK316877 mRNA Translation: BAH19585.1 AK317470 mRNA Translation: BAH20135.1 AY085589 mRNA Translation: AAM62810.1 |
PIRi | G84594 |
RefSeqi | NP_001031381.1, NM_001036304.1 [Q9C5E5-1] NP_565492.1, NM_127660.4 [Q9C5E5-2] NP_850007.1, NM_179676.3 [Q9C5E5-1] NP_973498.1, NM_201769.3 [Q9C5E5-2] |
Genome annotation databases
EnsemblPlantsi | AT2G20900.1; AT2G20900.1; AT2G20900 [Q9C5E5-1] AT2G20900.2; AT2G20900.2; AT2G20900 [Q9C5E5-2] AT2G20900.3; AT2G20900.3; AT2G20900 [Q9C5E5-2] AT2G20900.4; AT2G20900.4; AT2G20900 [Q9C5E5-1] |
GeneIDi | 816624 |
Gramenei | AT2G20900.1; AT2G20900.1; AT2G20900 [Q9C5E5-1] AT2G20900.2; AT2G20900.2; AT2G20900 [Q9C5E5-2] AT2G20900.3; AT2G20900.3; AT2G20900 [Q9C5E5-2] AT2G20900.4; AT2G20900.4; AT2G20900 [Q9C5E5-1] |
KEGGi | ath:AT2G20900 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC006234 Genomic DNA Translation: AAD20931.2 CP002685 Genomic DNA Translation: AEC07093.1 CP002685 Genomic DNA Translation: AEC07094.1 CP002685 Genomic DNA Translation: AEC07095.1 CP002685 Genomic DNA Translation: AEC07096.1 AF360300 mRNA Translation: AAK26010.1 BT000983 mRNA Translation: AAN41383.1 AK316877 mRNA Translation: BAH19585.1 AK317470 mRNA Translation: BAH20135.1 AY085589 mRNA Translation: AAM62810.1 |
PIRi | G84594 |
RefSeqi | NP_001031381.1, NM_001036304.1 [Q9C5E5-1] NP_565492.1, NM_127660.4 [Q9C5E5-2] NP_850007.1, NM_179676.3 [Q9C5E5-1] NP_973498.1, NM_201769.3 [Q9C5E5-2] |
3D structure databases
SMRi | Q9C5E5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 1977, 1 interactor |
IntActi | Q9C5E5, 1 interactor |
STRINGi | 3702.AT2G20900.1 |
PTM databases
iPTMneti | Q9C5E5 |
Proteomic databases
PaxDbi | Q9C5E5 |
PRIDEi | Q9C5E5 |
ProteomicsDBi | 224215 [Q9C5E5-1] |
Protocols and materials databases
DNASUi | 816624 |
Genome annotation databases
EnsemblPlantsi | AT2G20900.1; AT2G20900.1; AT2G20900 [Q9C5E5-1] AT2G20900.2; AT2G20900.2; AT2G20900 [Q9C5E5-2] AT2G20900.3; AT2G20900.3; AT2G20900 [Q9C5E5-2] AT2G20900.4; AT2G20900.4; AT2G20900 [Q9C5E5-1] |
GeneIDi | 816624 |
Gramenei | AT2G20900.1; AT2G20900.1; AT2G20900 [Q9C5E5-1] AT2G20900.2; AT2G20900.2; AT2G20900 [Q9C5E5-2] AT2G20900.3; AT2G20900.3; AT2G20900 [Q9C5E5-2] AT2G20900.4; AT2G20900.4; AT2G20900 [Q9C5E5-1] |
KEGGi | ath:AT2G20900 |
Organism-specific databases
Araporti | AT2G20900 |
TAIRi | locus:2051343, AT2G20900 |
Phylogenomic databases
eggNOGi | KOG1169, Eukaryota |
InParanoidi | Q9C5E5 |
OMAi | IDSWHIM |
OrthoDBi | 633642at2759 |
PhylomeDBi | Q9C5E5 |
Enzyme and pathway databases
BioCyci | ARA:AT2G20900-MONOMER |
Miscellaneous databases
PROi | PR:Q9C5E5 |
Gene expression databases
ExpressionAtlasi | Q9C5E5, baseline and differential |
Genevisiblei | Q9C5E5, AT |
Family and domain databases
Gene3Di | 3.40.50.10330, 1 hit |
InterProi | View protein in InterPro IPR017438, ATP-NAD_kinase_N IPR037607, DGK IPR000756, Diacylglycerol_kin_accessory IPR001206, Diacylglycerol_kinase_cat_dom IPR016961, Diacylglycerol_kinase_pln IPR016064, NAD/diacylglycerol_kinase_sf |
PANTHERi | PTHR11255, PTHR11255, 1 hit |
Pfami | View protein in Pfam PF00609, DAGK_acc, 1 hit PF00781, DAGK_cat, 1 hit |
PIRSFi | PIRSF030829, Diacylglycerol_kinase_pln, 1 hit |
SMARTi | View protein in SMART SM00045, DAGKa, 1 hit SM00046, DAGKc, 1 hit |
SUPFAMi | SSF111331, SSF111331, 1 hit |
PROSITEi | View protein in PROSITE PS50146, DAGK, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DGK5_ARATH | |
Accessioni | Q9C5E5Primary (citable) accession number: Q9C5E5 Secondary accession number(s): Q8LE70, Q9SKS9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 1, 2013 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 117 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - SIMILARITY comments
Index of protein domains and families