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UniProtKB - Q9C512 (MNS1_ARATH)
Protein
Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1
Gene
MNS1
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Functioni
Class I alpha-mannosidase essential for early N-glycan processing. Progressively trims alpha-1,2-linked mannose residues. Produces Man5GlcNAc2 from Man8GlcNAc2, but only Man6GlcNAc2 from Man9GlcNAc2. Has difficulty acting on the terminal mannose of the b-branch. Involved in root development and cell wall biosynthesis.
2 PublicationsCatalytic activityi
- 4 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = 4 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)1 PublicationEC:3.2.1.1131 Publication
- 3 H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3) = 3 β-D-mannose + N4-(α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-β-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 5A1,2)1 PublicationEC:3.2.1.1131 Publication
- H2O + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 9A1,2,3B1,2,3) = β-D-mannose + N4-(α-D-Man-(1→2)-α-D-Man-(1→2)-α-D-Man-(1→3)-[α-D-Man-(1→3)-[α-D-Man-(1→2)-α-D-Man-(1→6)]-α-D-Man-(1→6)]-β-D-Man-(1→4)-β-D-GlcNAc-(1→4)-α-D-GlcNAc)-L-asparaginyl-[protein] (N-glucan mannose isomer 8A1,2,3B1,3)1 PublicationEC:3.2.1.2091 Publication
Cofactori
Ca2+1 Publication, Mn2+1 Publication, Mg2+1 PublicationNote: Ca2+ or Mn2+. Mg2+ can be used to a lesser extent.1 Publication
Activity regulationi
Inhibited by kifunensine and 1-deoxymannojirimycin, but not by swainsonine.2 Publications
pH dependencei
Optimum pH is 6.0. Stable from pH 4.5 to 6.5.1 Publication
Temperature dependencei
Optimum temperature is 25 degrees Celsius.1 Publication
: protein glycosylation Pathwayi
This protein is involved in the pathway protein glycosylation, which is part of Protein modification.CuratedView all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 179 | Proton donorBy similarity | 1 | |
Active sitei | 312 | By similarity | 1 | |
Active sitei | 423 | Proton donorBy similarity | 1 | |
Active sitei | 445 | By similarity | 1 | |
Metal bindingi | 529 | CalciumBy similarity | 1 |
GO - Molecular functioni
- alpha-mannosidase activity Source: TAIR
- calcium ion binding Source: InterPro
- mannosyl-oligosaccharide 1,2-alpha-mannosidase activity Source: GO_Central
GO - Biological processi
- carbohydrate metabolic process Source: InterPro
- N-glycan processing Source: TAIR
- protein glycosylation Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Hydrolase |
Ligand | Calcium, Magnesium, Manganese, Metal-binding |
Enzyme and pathway databases
BioCyci | ARA:AT1G51590-MONOMER |
BRENDAi | 3.2.1.113, 399 |
UniPathwayi | UPA00378 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:MNS1 Synonyms:MANIB Ordered Locus Names:At1g51590 ORF Names:F19C24.18, F5D21.1 |
Organismi | Arabidopsis thaliana (Mouse-ear cress) |
Taxonomic identifieri | 3702 [NCBI] |
Taxonomic lineagei | Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliopsida › eudicotyledons › Gunneridae › Pentapetalae › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis |
Proteomesi |
|
Organism-specific databases
Araporti | AT1G51590 |
TAIRi | locus:2017597, AT1G51590 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane 2 Publications; Single-pass type II membrane protein 2 Publications
Endoplasmic reticulum
- endoplasmic reticulum Source: GO_Central
Endosome
- endosome Source: TAIR
Golgi apparatus
- Golgi apparatus Source: TAIR
- Golgi cis cisterna Source: TAIR
- Golgi membrane Source: GO_Central
- trans-Golgi network Source: TAIR
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 27 | CytoplasmicSequence analysisAdd BLAST | 27 | |
Transmembranei | 28 – 47 | Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST | 20 | |
Topological domaini | 48 – 560 | LumenalSequence analysisAdd BLAST | 513 |
Keywords - Cellular componenti
Golgi apparatus, MembranePathology & Biotechi
Disruption phenotypei
No visible phenotype; due the redundancy with MNS2. Lack of complex N-glycans, shorter roots and increased lateral root formation in mns1 and mns2 double mutants.2 Publications
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000397933 | 1 – 560 | Mannosyl-oligosaccharide 1,2-alpha-mannosidase MNS1Add BLAST | 560 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 326 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 377 ↔ 409 | By similarity | ||
Glycosylationi | 459 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9C512 |
PRIDEi | Q9C512 |
ProteomicsDBi | 251344 [Q9C512-1] |
Expressioni
Tissue specificityi
Expressed in flowers, siliques, stems, leaves, roots, pollen grains, shoot apical meristems, hypocotyls and upper region of the root.2 Publications
Gene expression databases
ExpressionAtlasi | Q9C512, baseline and differential |
Genevisiblei | Q9C512, AT |
Interactioni
Protein-protein interaction databases
BioGRIDi | 26809, 2 interactors |
STRINGi | 3702.AT1G51590.1 |
Family & Domainsi
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 47 – 80 | Sequence analysisAdd BLAST | 34 |
Sequence similaritiesi
Belongs to the glycosyl hydrolase 47 family.Curated
Keywords - Domaini
Coiled coil, Signal-anchor, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2204, Eukaryota |
InParanoidi | Q9C512 |
OMAi | QKVMNIR |
PhylomeDBi | Q9C512 |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketIsoform 1 (identifier: Q9C512-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MARSRSISGY GIWKYLNPAY YLRRPRRLAL LFIVFVSVSM LVWDRINLAR
60 70 80 90 100
EHEVEVFKLN EEVSRLEQML EELNGGVGNK PLKTLKDAPE DPVDKQRRQK
110 120 130 140 150
VKEAMIHAWS SYEKYAWGKD ELQPRTKDGT DSFGGLGATM VDSLDTLYIM
160 170 180 190 200
GLDEQFQKAR EWVASSLDFD KDYDASMFET TIRVVGGLLS AYDLSGDKMF
210 220 230 240 250
LEKAKDIADR LLPAWNTPTG IPYNIINLRN GNAHNPSWAA GGDSILADSG
260 270 280 290 300
TEQLEFIALS QRTGDPKYQQ KVEKVITELN KNFPADGLLP IYINPDNANP
310 320 330 340 350
SYSTTTFGAM GDSFYEYLLK VWVQGNKTSA VKPYRDMWEK SMKGLLSLVK
360 370 380 390 400
KSTPSSFTYI CEKNGNNLID KMDELACFAP GMLALGASGY GPDEEKKFLS
410 420 430 440 450
LAGELAWTCY NFYQSTPTKL AGENYFFTAG QDMSVGTSWN ILRPETVESL
460 470 480 490 500
FYLWRLTGNK TYQEWGWNIF QAFEKNSRVE SGYVGLKDVN TGAKDNKMQS
510 520 530 540 550
FFLAETLKYL YLLFSPSSVI SLDEWVFNTE AHPLKIVARN DPRKPTIALR
560
QRKFGHQINV
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039724 | 1 – 104 | Missing in isoform 2. CuratedAdd BLAST | 104 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC024261 Genomic DNA Translation: AAG52623.1 AC025294 Genomic DNA Translation: AAG50876.1 CP002684 Genomic DNA Translation: AEE32686.1 CP002684 Genomic DNA Translation: AEE32687.1 AY081353 mRNA Translation: AAL91242.1 AY128845 mRNA Translation: AAM91245.1 |
PIRi | E96554 |
RefSeqi | NP_001031171.1, NM_001036094.1 [Q9C512-2] NP_175570.1, NM_104037.4 [Q9C512-1] |
Genome annotation databases
EnsemblPlantsi | AT1G51590.1; AT1G51590.1; AT1G51590 [Q9C512-1] AT1G51590.2; AT1G51590.2; AT1G51590 [Q9C512-2] |
GeneIDi | 841584 |
Gramenei | AT1G51590.1; AT1G51590.1; AT1G51590 [Q9C512-1] AT1G51590.2; AT1G51590.2; AT1G51590 [Q9C512-2] |
KEGGi | ath:AT1G51590 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AC024261 Genomic DNA Translation: AAG52623.1 AC025294 Genomic DNA Translation: AAG50876.1 CP002684 Genomic DNA Translation: AEE32686.1 CP002684 Genomic DNA Translation: AEE32687.1 AY081353 mRNA Translation: AAL91242.1 AY128845 mRNA Translation: AAM91245.1 |
PIRi | E96554 |
RefSeqi | NP_001031171.1, NM_001036094.1 [Q9C512-2] NP_175570.1, NM_104037.4 [Q9C512-1] |
3D structure databases
SMRi | Q9C512 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 26809, 2 interactors |
STRINGi | 3702.AT1G51590.1 |
Protein family/group databases
CAZyi | GH47, Glycoside Hydrolase Family 47 |
Proteomic databases
PaxDbi | Q9C512 |
PRIDEi | Q9C512 |
ProteomicsDBi | 251344 [Q9C512-1] |
Genome annotation databases
EnsemblPlantsi | AT1G51590.1; AT1G51590.1; AT1G51590 [Q9C512-1] AT1G51590.2; AT1G51590.2; AT1G51590 [Q9C512-2] |
GeneIDi | 841584 |
Gramenei | AT1G51590.1; AT1G51590.1; AT1G51590 [Q9C512-1] AT1G51590.2; AT1G51590.2; AT1G51590 [Q9C512-2] |
KEGGi | ath:AT1G51590 |
Organism-specific databases
Araporti | AT1G51590 |
TAIRi | locus:2017597, AT1G51590 |
Phylogenomic databases
eggNOGi | KOG2204, Eukaryota |
InParanoidi | Q9C512 |
OMAi | QKVMNIR |
PhylomeDBi | Q9C512 |
Enzyme and pathway databases
UniPathwayi | UPA00378 |
BioCyci | ARA:AT1G51590-MONOMER |
BRENDAi | 3.2.1.113, 399 |
Miscellaneous databases
PROi | PR:Q9C512 |
Gene expression databases
ExpressionAtlasi | Q9C512, baseline and differential |
Genevisiblei | Q9C512, AT |
Family and domain databases
Gene3Di | 1.50.10.10, 1 hit |
InterProi | View protein in InterPro IPR012341, 6hp_glycosidase-like_sf IPR001382, Glyco_hydro_47 IPR036026, Seven-hairpin_glycosidases |
Pfami | View protein in Pfam PF01532, Glyco_hydro_47, 1 hit |
PRINTSi | PR00747, GLYHDRLASE47 |
SUPFAMi | SSF48225, SSF48225, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | MNS1_ARATH | |
Accessioni | Q9C512Primary (citable) accession number: Q9C512 Secondary accession number(s): Q2V4H4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 5, 2010 |
Last sequence update: | June 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 130 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Plant Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Glycosyl hydrolases
Classification of glycosyl hydrolase families and list of entries - Arabidopsis thaliana
Arabidopsis thaliana: entries and gene names - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families