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Protein

pre-mRNA 3' end processing protein WDR33

Gene

WDR33

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Essential for both cleavage and polyadenylation of pre-mRNA 3' ends.1 Publication

GO - Molecular functioni

GO - Biological processi

  • mRNA 3'-end processing Source: Reactome
  • mRNA export from nucleus Source: Reactome
  • mRNA polyadenylation Source: GO_Central
  • mRNA splicing, via spliceosome Source: Reactome
  • postreplication repair Source: UniProtKB
  • spermatogenesis Source: UniProtKB
  • termination of RNA polymerase II transcription Source: Reactome

Keywordsi

Biological processmRNA processing

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
R-HSA-77595 Processing of Intronless Pre-mRNAs
SignaLinkiQ9C0J8

Names & Taxonomyi

Protein namesi
Recommended name:
pre-mRNA 3' end processing protein WDR33
Alternative name(s):
WD repeat-containing protein 33
WD repeat-containing protein of 146 kDa1 Publication
Gene namesi
Name:WDR33
Synonyms:WDC1461 Publication
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 2

Organism-specific databases

EuPathDBiHostDB:ENSG00000136709.11
HGNCiHGNC:25651 WDR33
MIMi618082 gene
neXtProtiNX_Q9C0J8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000136709
PharmGKBiPA134943440

Polymorphism and mutation databases

BioMutaiWDR33
DMDMi209572695

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources
ChainiPRO_00000513822 – 1336pre-mRNA 3' end processing protein WDR33Add BLAST1335

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1
Modified residuei7PhosphoserineCombined sources1
Modified residuei46N6-acetyllysineCombined sources1
Cross-linki526Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki530Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Cross-linki560Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei782Omega-N-methylarginineCombined sources1
Modified residuei915Asymmetric dimethylarginineCombined sources1
Modified residuei987Omega-N-methylarginineBy similarity1
Modified residuei1035Omega-N-methylarginineBy similarity1
Modified residuei1210PhosphoserineCombined sources1
Modified residuei1262Omega-N-methylarginineBy similarity1
Modified residuei1315Asymmetric dimethylarginine; alternateCombined sources1
Modified residuei1315Omega-N-methylarginine; alternateCombined sources1

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9C0J8
MaxQBiQ9C0J8
PaxDbiQ9C0J8
PeptideAtlasiQ9C0J8
PRIDEiQ9C0J8
ProteomicsDBi80062
80063 [Q9C0J8-2]
80064 [Q9C0J8-3]

PTM databases

iPTMnetiQ9C0J8
PhosphoSitePlusiQ9C0J8
SwissPalmiQ9C0J8

Expressioni

Tissue specificityi

Most highly expressed in testis.1 Publication

Gene expression databases

BgeeiENSG00000136709 Expressed in 203 organ(s), highest expression level in cerebral cortex
CleanExiHS_WDR33
ExpressionAtlasiQ9C0J8 baseline and differential
GenevisibleiQ9C0J8 HS

Organism-specific databases

HPAiHPA026897
HPA046527

Interactioni

Subunit structurei

Component of the cleavage and polyadenylation specificity factor (CPSF) module of the pre-mRNA 3'-end processing complex. Interacts with CPSF3/CPSF73.1 Publication

Protein-protein interaction databases

BioGridi120620, 67 interactors
IntActiQ9C0J8, 32 interactors
MINTiQ9C0J8
STRINGi9606.ENSP00000325377

Structurei

Secondary structure

11336
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9C0J8
SMRiQ9C0J8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati117 – 156WD 1Add BLAST40
Repeati159 – 198WD 2Add BLAST40
Repeati200 – 239WD 3Add BLAST40
Repeati242 – 283WD 4Add BLAST42
Repeati286 – 325WD 5Add BLAST40
Repeati329 – 369WD 6Add BLAST41
Repeati373 – 412WD 7Add BLAST40
Domaini618 – 770Collagen-likeAdd BLAST153

Keywords - Domaini

Collagen, Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0284 Eukaryota
COG2319 LUCA
GeneTreeiENSGT00730000111130
HOGENOMiHOG000148601
HOVERGENiHBG062379
KOiK15542
OMAiNQEGQSA
OrthoDBiEOG091G048U
PhylomeDBiQ9C0J8
TreeFamiTF317659

Family and domain databases

Gene3Di2.130.10.10, 3 hits
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 5 hits
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit

Sequences (3+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 3 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9C0J8-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MATEIGSPPR FFHMPRFQHQ APRQLFYKRP DFAQQQAMQQ LTFDGKRMRK
60 70 80 90 100
AVNRKTIDYN PSVIKYLENR IWQRDQRDMR AIQPDAGYYN DLVPPIGMLN
110 120 130 140 150
NPMNAVTTKF VRTSTNKVKC PVFVVRWTPE GRRLVTGASS GEFTLWNGLT
160 170 180 190 200
FNFETILQAH DSPVRAMTWS HNDMWMLTAD HGGYVKYWQS NMNNVKMFQA
210 220 230 240 250
HKEAIREASF SPTDNKFATC SDDGTVRIWD FLRCHEERIL RGHGADVKCV
260 270 280 290 300
DWHPTKGLVV SGSKDSQQPI KFWDPKTGQS LATLHAHKNT VMEVKLNLNG
310 320 330 340 350
NWLLTASRDH LCKLFDIRNL KEELQVFRGH KKEATAVAWH PVHEGLFASG
360 370 380 390 400
GSDGSLLFWH VGVEKEVGGM EMAHEGMIWS LAWHPLGHIL CSGSNDHTSK
410 420 430 440 450
FWTRNRPGDK MRDRYNLNLL PGMSEDGVEY DDLEPNSLAV IPGMGIPEQL
460 470 480 490 500
KLAMEQEQMG KDESNEIEMT IPGLDWGMEE VMQKDQKKVP QKKVPYAKPI
510 520 530 540 550
PAQFQQAWMQ NKVPIPAPNE VLNDRKEDIK LEEKKKTQAE IEQEMATLQY
560 570 580 590 600
TNPQLLEQLK IERLAQKQVE QIQPPPSSGT PLLGPQPFPG QGPMSQIPQG
610 620 630 640 650
FQQPHPSQQM PMNMAQMGPP GPQGQFRPPG PQGQMGPQGP PLHQGGGGPQ
660 670 680 690 700
GFMGPQGPQG PPQGLPRPQD MHGPQGMQRH PGPHGPLGPQ GPPGPQGSSG
710 720 730 740 750
PQGHMGPQGP PGPQGHIGPQ GPPGPQGHLG PQGPPGTQGM QGPPGPRGMQ
760 770 780 790 800
GPPHPHGIQG GPGSQGIQGP VSQGPLMGLN PRGMQGPPGP RENQGPAPQG
810 820 830 840 850
MIMGHPPQEM RGPHPPGGLL GHGPQEMRGP QEIRGMQGPP PQGSMLGPPQ
860 870 880 890 900
ELRGPPGSQS QQGPPQGSLG PPPQGGMQGP PGPQGQQNPA RGPHPSQGPI
910 920 930 940 950
PFQQQKTPLL GDGPRAPFNQ EGQSTGPPPL IPGLGQQGAQ GRIPPLNPGQ
960 970 980 990 1000
GPGPNKGDSR GPPNHHMGPM SERRHEQSGG PEHGPERGPF RGGQDCRGPP
1010 1020 1030 1040 1050
DRRGPHPDFP DDFSRPDDFH PDKRFGHRLR EFEGRGGPLP QEEKWRRGGP
1060 1070 1080 1090 1100
GPPFPPDHRE FSEGDGRGAA RGPPGAWEGR RPGDERFPRD PEDPRFRGRR
1110 1120 1130 1140 1150
EESFRRGAPP RHEGRAPPRG RDGFPGPEDF GPEENFDASE EAARGRDLRG
1160 1170 1180 1190 1200
RGRGTPRGGR KGLLPTPDEF PRFEGGRKPD SWDGNREPGP GHEHFRDTPR
1210 1220 1230 1240 1250
PDHPPHDGHS PASRERSSSL QGMDMASLPP RKRPWHDGPG TSEHREMEAP
1260 1270 1280 1290 1300
GGPSEDRGGK GRGGPGPAQR VPKSGRSSSL DGEHHDGYHR DEPFGGPPGS
1310 1320 1330
GTPSRGGRSG SNWGRGSNMN SGPPRRGASR GGGRGR
Length:1,336
Mass (Da):145,891
Last modified:October 14, 2008 - v2
Checksum:iDE45F510F93BB783
GO
Isoform 2 (identifier: Q9C0J8-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     209-326: SFSPTDNKFA...IRNLKEELQV → RFIHNIPFSV...YFIPNKEFSL
     327-1336: Missing.

Show »
Length:326
Mass (Da):38,294
Checksum:i1F1F704B877F7904
GO
Isoform 3 (identifier: Q9C0J8-3) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     242-257: GHGADVKCVDWHPTKG → DTCFHHCRCYFLSVKR
     258-326: Missing.
     327-1336: Missing.

Note: No experimental confirmation available.
Show »
Length:257
Mass (Da):30,288
Checksum:i2235A458B412633E
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
C9J8B4C9J8B4_HUMAN
pre-mRNA 3' end-processing protein ...
WDR33
205Annotation score:
B9A053B9A053_HUMAN
pre-mRNA 3' end-processing protein ...
WDR33
118Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti113T → A in BAD97039 (Ref. 3) Curated1
Sequence conflicti715G → S in BAB32435 (PubMed:11162572).Curated1
Isoform 2 (identifier: Q9C0J8-2)
Sequence conflicti274R → Q in BAD97039 (Ref. 3) Curated1
Sequence conflicti306F → S in AAH05401 (PubMed:15489334).Curated1

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_04671733A → S. Corresponds to variant dbSNP:rs11557686Ensembl.1
Natural variantiVAR_053427711P → R. Corresponds to variant dbSNP:rs12615078Ensembl.1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_041333209 – 326SFSPT…EELQV → RFIHNIPFSVVPIVMVKLFS KCILGAEMHGLCQFLGNFLH PINTIFFFVFTHSPFCWHLS EVVLSRYQPLQYVRDVLSAA FCTGFLFSFMINNVYTLFLF IIYCVRQEYFIPNKEFSL in isoform 2. 3 PublicationsAdd BLAST118
Alternative sequenceiVSP_042684242 – 257GHGAD…HPTKG → DTCFHHCRCYFLSVKR in isoform 3. 1 PublicationAdd BLAST16
Alternative sequenceiVSP_042685258 – 326Missing in isoform 3. 1 PublicationAdd BLAST69
Alternative sequenceiVSP_041334327 – 1336Missing in isoform 2 and isoform 3. 3 PublicationsAdd BLAST1010

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044749 mRNA Translation: BAB32435.1
AK002156 mRNA Translation: BAA92113.1
AK223319 mRNA Translation: BAD97039.1
AC006011 Genomic DNA Translation: AAX82033.1
CH471103 Genomic DNA Translation: EAW95342.1
CH471103 Genomic DNA Translation: EAW95343.1
BC005401 mRNA Translation: AAH05401.1 Different termination.
BC013990 mRNA Translation: AAH13990.1
BC068484 mRNA Translation: AAH68484.1
AL834365 mRNA Translation: CAH10688.1
CCDSiCCDS2150.1 [Q9C0J8-1]
CCDS42746.1 [Q9C0J8-3]
CCDS46407.1 [Q9C0J8-2]
RefSeqiNP_001006623.1, NM_001006622.2 [Q9C0J8-2]
NP_001006624.1, NM_001006623.2 [Q9C0J8-3]
NP_060853.3, NM_018383.4 [Q9C0J8-1]
XP_011509738.1, XM_011511436.1 [Q9C0J8-1]
UniGeneiHs.554831

Genome annotation databases

EnsembliENST00000322313; ENSP00000325377; ENSG00000136709 [Q9C0J8-1]
ENST00000393006; ENSP00000376730; ENSG00000136709 [Q9C0J8-3]
ENST00000409658; ENSP00000387186; ENSG00000136709 [Q9C0J8-2]
GeneIDi55339
KEGGihsa:55339
UCSCiuc002tpg.3 human [Q9C0J8-1]

Keywords - Coding sequence diversityi

Alternative splicing, Polymorphism

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB044749 mRNA Translation: BAB32435.1
AK002156 mRNA Translation: BAA92113.1
AK223319 mRNA Translation: BAD97039.1
AC006011 Genomic DNA Translation: AAX82033.1
CH471103 Genomic DNA Translation: EAW95342.1
CH471103 Genomic DNA Translation: EAW95343.1
BC005401 mRNA Translation: AAH05401.1 Different termination.
BC013990 mRNA Translation: AAH13990.1
BC068484 mRNA Translation: AAH68484.1
AL834365 mRNA Translation: CAH10688.1
CCDSiCCDS2150.1 [Q9C0J8-1]
CCDS42746.1 [Q9C0J8-3]
CCDS46407.1 [Q9C0J8-2]
RefSeqiNP_001006623.1, NM_001006622.2 [Q9C0J8-2]
NP_001006624.1, NM_001006623.2 [Q9C0J8-3]
NP_060853.3, NM_018383.4 [Q9C0J8-1]
XP_011509738.1, XM_011511436.1 [Q9C0J8-1]
UniGeneiHs.554831

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
6BLYelectron microscopy3.36B1-572[»]
6BM0electron microscopy3.80B1-572[»]
6DNHelectron microscopy3.40B1-572[»]
6F9NX-ray2.50B35-410[»]
6FBSelectron microscopy3.07B1-410[»]
6FUWelectron microscopy3.07B1-410[»]
ProteinModelPortaliQ9C0J8
SMRiQ9C0J8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi120620, 67 interactors
IntActiQ9C0J8, 32 interactors
MINTiQ9C0J8
STRINGi9606.ENSP00000325377

PTM databases

iPTMnetiQ9C0J8
PhosphoSitePlusiQ9C0J8
SwissPalmiQ9C0J8

Polymorphism and mutation databases

BioMutaiWDR33
DMDMi209572695

Proteomic databases

EPDiQ9C0J8
MaxQBiQ9C0J8
PaxDbiQ9C0J8
PeptideAtlasiQ9C0J8
PRIDEiQ9C0J8
ProteomicsDBi80062
80063 [Q9C0J8-2]
80064 [Q9C0J8-3]

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000322313; ENSP00000325377; ENSG00000136709 [Q9C0J8-1]
ENST00000393006; ENSP00000376730; ENSG00000136709 [Q9C0J8-3]
ENST00000409658; ENSP00000387186; ENSG00000136709 [Q9C0J8-2]
GeneIDi55339
KEGGihsa:55339
UCSCiuc002tpg.3 human [Q9C0J8-1]

Organism-specific databases

CTDi55339
EuPathDBiHostDB:ENSG00000136709.11
GeneCardsiWDR33
HGNCiHGNC:25651 WDR33
HPAiHPA026897
HPA046527
MIMi618082 gene
neXtProtiNX_Q9C0J8
OpenTargetsiENSG00000136709
PharmGKBiPA134943440
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG0284 Eukaryota
COG2319 LUCA
GeneTreeiENSGT00730000111130
HOGENOMiHOG000148601
HOVERGENiHBG062379
KOiK15542
OMAiNQEGQSA
OrthoDBiEOG091G048U
PhylomeDBiQ9C0J8
TreeFamiTF317659

Enzyme and pathway databases

ReactomeiR-HSA-109688 Cleavage of Growing Transcript in the Termination Region
R-HSA-159231 Transport of Mature mRNA Derived from an Intronless Transcript
R-HSA-72163 mRNA Splicing - Major Pathway
R-HSA-72187 mRNA 3'-end processing
R-HSA-77595 Processing of Intronless Pre-mRNAs
SignaLinkiQ9C0J8

Miscellaneous databases

ChiTaRSiWDR33 human
GeneWikiiWDR33
GenomeRNAii55339
PROiPR:Q9C0J8
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000136709 Expressed in 203 organ(s), highest expression level in cerebral cortex
CleanExiHS_WDR33
ExpressionAtlasiQ9C0J8 baseline and differential
GenevisibleiQ9C0J8 HS

Family and domain databases

Gene3Di2.130.10.10, 3 hits
InterProiView protein in InterPro
IPR015943 WD40/YVTN_repeat-like_dom_sf
IPR001680 WD40_repeat
IPR017986 WD40_repeat_dom
IPR036322 WD40_repeat_dom_sf
PfamiView protein in Pfam
PF00400 WD40, 5 hits
SMARTiView protein in SMART
SM00320 WD40, 7 hits
SUPFAMiSSF50978 SSF50978, 1 hit
PROSITEiView protein in PROSITE
PS50082 WD_REPEATS_2, 6 hits
PS50294 WD_REPEATS_REGION, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiWDR33_HUMAN
AccessioniPrimary (citable) accession number: Q9C0J8
Secondary accession number(s): Q05DP8
, Q53FG9, Q587J1, Q69YF7, Q6NUQ0, Q9NUL1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 3, 2002
Last sequence update: October 14, 2008
Last modified: November 7, 2018
This is version 159 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 2
    Human chromosome 2: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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